Restriction map for
pFN21AB4351
(Circular) (Six-base) MAPSORT of: pFN21AB4351.seq Check: 474 from: 1 to: 5785
pFN21AB4351 5785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 2044
Size: 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 2436 3912 4285 1563
Size: 873 1476 373 3063
Fragments arranged by size:
3063 1476 873 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 3848 5232 5500 1632
Size: 589 1627 1384 268 1917
Fragments arranged by size:
1917 1627 1384 589 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 2573 4684 5661 1203
Size: 1370 2111 977 1327
Fragments arranged by size:
2111 1370 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2926 3528 5100 2926
Size: 602 1572 3611
Fragments arranged by size:
3611 1572 602
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5785
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2605 2773 3094 3320 3472 5581 1493
Size: 1112 168 321 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 321 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2959 3110 1870
Size: 73 1016 151 4545
Fragments arranged by size:
4545 1016 151 73
BamHI G'GATC_C
Cuts at: 2738 3115 3482 2738
Size: 377 367 5041
Fragments arranged by size:
5041 377 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2821 3106 4561 618
Size: 325 217 627 318 716 285 1455 1842
Fragments arranged by size:
1842 1455 716 627 325 318 285 217
BanII G_rGCy'C
Cuts at: 1391 2036 2590 2973 3104 1391
Size: 645 554 383 131 4072
Fragments arranged by size:
4072 645 554 383 131
BbeI G_GCGC'C
Cuts at: 2109 2109
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 2760 928
Size: 971 461 400 3953
Fragments arranged by size:
3953 971 461 400
BbvCI CC'TCA_GC
Cuts at: 2366 2366
Size: 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3068 3677 4887 3068
Size: 609 1210 3966
Fragments arranged by size:
3966 1210 609
BclI T'GATC_A
Cuts at: 1548 2539 1548
Size: 991 4794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4396 136
Size: 107 122 71 631 605 337 2387 1525
Fragments arranged by size:
2387 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2335 2496 3080 5780 2335
Size: 161 584 2700 2340
Fragments arranged by size:
2700 2340 584 161
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3846 5002 1790
Size: 246 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 246
BmgBI CAC'GTC
Cuts at: 1318 2065 1318
Size: 747 5038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2574 2963 4445 1239
Size: 1335 389 1482 2579
Fragments arranged by size:
2579 1482 1335 389
Bpu10I CC'TnA_GC
Cuts at: 2366 2366
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2477 3910 4775 5073 2438
Size: 39 1433 865 298 3150
Fragments arranged by size:
3150 1433 865 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 2222 2743 3079 5779 2222
Size: 521 336 2700 2228
Fragments arranged by size:
2700 2228 521 336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 3592 3974 4609
Size: 53 83 186 936 573 1486 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1486 1451 936 635 573 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 1163
Size: 573 690 4522
Fragments arranged by size:
4522 690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2187 2281 3846 3931 5002 2126
Size: 61 94 1565 85 1071 2909
Fragments arranged by size:
2909 1565 1071 94 85 61
BsmI GAATG_Cn'
Cuts at: 2652 3296 3389 2652
Size: 644 93 5048
Fragments arranged by size:
5048 644 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2126 2187 2281 2590 2973 3104
Size: 399 246 90 61 94 309 383 131
Cuts at: 3104 3846 3931 5002 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 309 246 131
94 90 85 61
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 2475 3140 844
Size: 1200 431 665 3489
Fragments arranged by size:
3489 1200 665 431
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2377 4280 4454 65
Size: 1183 1129 1903 174 1396
Fragments arranged by size:
1903 1396 1183 1129 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2121 794
Size: 1327 4458
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2863 2863
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2335 2400 2496 2527 2738 3080 3115 3482
Size: 363 65 96 31 211 342 35 367
Cuts at: 3482 3867 3884 5336 5422 5434 5780 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 363 346 342 211
96 86 65 35 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 2210 3021 2210
Size: 811 4974
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 2825 513
Size: 552 939 43 778 3473
Fragments arranged by size:
3473 939 778 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2959 487
Size: 683 56 1733 3313
Fragments arranged by size:
3313 1733 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 4095 4115 3301
Size: 794 20 4971
Fragments arranged by size:
4971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 2778 3089 2778
Size: 311 5474
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 3232 4121
Size: 54 1425 50 275 18 738 664 889
Cuts at: 4121 5750 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 738 664 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2808 3045 3715 1353
Size: 1455 237 670 3423
Fragments arranged by size:
3423 1455 670 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2574 2963 3848 4445 5232 5500
Size: 393 589 353 389 885 597 787 268
Cuts at: 5500 1239
Size: 1524
Fragments arranged by size:
1524 885 787 597 589 393 389 353
268
EcoO109I rG'GnC_Cy
Cuts at: 2320 2320
Size: 5785
EcoRI G'AATT_C
Cuts at: 2605 3094 3472 2605
Size: 489 378 4918
Fragments arranged by size:
4918 489 378
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2109 4932 1678
Size: 431 2823 2531
Fragments arranged by size:
2823 2531 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2145 2177 2624 2656 4426
Size: 32 135 32 573 32 447 32 1770
Cuts at: 4426 4458 4500 4532 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1770 573 447 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2886 3129 3375 669
Size: 988 281 205 743 243 246 3079
Fragments arranged by size:
3079 988 743 281 246 243 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2105 2105
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 4899 5083 1305
Size: 1182 2412 184 2007
Fragments arranged by size:
2412 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 3225
Fragments arranged by size:
3225 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4102 4261 518
Size: 648 2577 359 159 2042
Fragments arranged by size:
2577 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2713 3201 3878 5026 5271 1606
Size: 443 664 488 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 488 443 245
NarI GG'CG_CC
Cuts at: 2106 2106
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 2825 513
Size: 552 939 821 3473
Fragments arranged by size:
3473 939 821 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2577 2725 1108
Size: 1469 148 4168
Fragments arranged by size:
4168 1469 148
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2320
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2713 2713
Size: 5785
SacII CC_GC'GG
Cuts at: 2050 2050
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2643 3133 4268 4949 5140 826
Size: 219 1598 490 1135 681 191 1471
Fragments arranged by size:
1598 1471 1135 681 490 219 191
SfoI GGC'GCC
Cuts at: 2107 2107
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2492 3889 4790 5052 820
Size: 197 926 510 39 1397 901 262 1553
Fragments arranged by size:
1553 1397 926 901 510 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 2825 3224 513
Size: 552 939 606 215 399 3074
Fragments arranged by size:
3074 939 606 552 399 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2949 3812 3829 3982 4167 4612 325
Size: 1255 1369 863 17 153 185 445 1498
Fragments arranged by size:
1498 1369 1255 863 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2314 4031 5743
Size: 274 80 33 51 1443 337 1717 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1717 1712 1443 337 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI