Restriction map for
pFN21AB4334
(Circular) (Six-base) MAPSORT of: pFN21AB4334.seq Check: 4217 from: 1 to: 6124
pFN21AB4334 6124 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 3423 4251 4624 1563
Size: 1860 828 373 3063
Fragments arranged by size:
3063 1860 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 4187 5571 5839 1632
Size: 1100 1455 1384 268 1917
Fragments arranged by size:
1917 1455 1384 1100 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2103 2925 5023 6000 1203
Size: 900 822 2098 977 1327
Fragments arranged by size:
2098 1327 977 900 822
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 2386 3355 4853 2386
Size: 969 1498 3657
Fragments arranged by size:
3657 1498 969
AleI CACnn'nnGTG
Cuts at: 1166 2849 1166
Size: 1683 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 3867 5439 3867
Size: 1572 4552
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 3172 3433 3659 3811 5920 1493
Size: 1679 261 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 261 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2662 2837 3228 3449 1870
Size: 73 153 566 175 391 221 4545
Fragments arranged by size:
4545 566 391 221 175 153 73
BamHI G'GATC_C
Cuts at: 2307 3454 3821 2307
Size: 1147 367 4610
Fragments arranged by size:
4610 1147 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 2687 3445 4900 618
Size: 325 217 627 569 331 758 1455 1842
Fragments arranged by size:
1842 1455 758 627 569 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2463 2628 3443 1391
Size: 1072 165 815 4072
Fragments arranged by size:
4072 1072 815 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2423 2668 3284 3291 928
Size: 971 524 245 616 7 3761
Fragments arranged by size:
3761 971 616 524 245 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3323 3357 4315 4349 1903
Size: 34 1386 34 958 34 3678
Fragments arranged by size:
3678 1386 958 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2729 4016 5226 2729
Size: 1287 1210 3627
Fragments arranged by size:
3627 1287 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BfrBI ATG'CAT
Cuts at: 2487 2816 2487
Size: 329 5795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 2361 4185 5341 1790
Size: 571 1824 1156 2573
Fragments arranged by size:
2573 1824 1156 571
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3855 4813 442
Size: 416 53 83 399 24 2438 958 1753
Fragments arranged by size:
2438 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 3243 2852
Size: 391 5733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2990 4784 1239
Size: 1751 1794 2579
Fragments arranged by size:
2579 1794 1751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5114 5412 4249
Size: 865 298 4961
Fragments arranged by size:
4961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2665 2882 3151 4787 882
Size: 617 1166 217 269 1636 2219
Fragments arranged by size:
2219 1636 1166 617 269 217
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 2201 6118 2201
Size: 3917 2207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2606 4557 4955 5229 5376 5787 2606
Size: 1951 398 274 147 411 2943
Fragments arranged by size:
2943 1951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2394 2424 1178
Size: 30 165 30 991 30 4878
Fragments arranged by size:
4878 991 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3307 1186
Size: 2121 4003
BseYI C'CCAG_C
Cuts at: 5327 5823 5327
Size: 496 5628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 1163
Size: 573 776 4775
Fragments arranged by size:
4775 776 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 2652 4185 4270 5341 2628
Size: 24 1533 85 1071 3411
Fragments arranged by size:
3411 1533 1071 85 24
BsmI GAATG_Cn'
Cuts at: 3346 3635 3728 3346
Size: 289 93 5742
Fragments arranged by size:
5742 289 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2114 2114
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2463 2628 2652 3443 4185 4270
Size: 399 571 102 165 24 791 742 85
Cuts at: 4270 5341 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 791 742 571 399 165 102
85 24
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3479 844
Size: 2635 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2822 794
Size: 2028 4096
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 2307 3454 3821 4206 4223 5675 5761 5773
Size: 335 1147 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1147 385 367 346 335 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2498 2498
Size: 6124
BtgI C'CryG_G
Cuts at: 513 1065 2559 513
Size: 552 1494 4078
Fragments arranged by size:
4078 1494 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2194 3099 487
Size: 683 56 968 905 3512
Fragments arranged by size:
3512 968 905 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4434 4454 3640
Size: 794 20 5310
Fragments arranged by size:
5310 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089
Size: 54 1425 50 275 18 1741 889 1629
Cuts at: 6089 8
Size: 43
Fragments arranged by size:
1741 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2717 2998 4054 1353
Size: 1364 281 1056 3423
Fragments arranged by size:
3423 1364 1056 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 2626
Size: 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2732 2990 4187 4784 5571 5839 1239
Size: 393 1100 258 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 1100 787 597 393 268 258
EcoNI CCTnn'n_nnAGG
Cuts at: 3231 3231
Size: 6124
EcoO109I rG'GnC_Cy
Cuts at: 2011 2177 3008 2011
Size: 166 831 5127
Fragments arranged by size:
5127 831 166
EcoRI G'AATT_C
Cuts at: 3172 3433 3811 3172
Size: 261 378 5485
Fragments arranged by size:
5485 378 261
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 5271 1678
Size: 3593 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 3341 3373 4765
Size: 32 135 32 800 32 937 32 1392
Cuts at: 4765 4797 4839 4871 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1392 937 800 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2986 3111 3421 3468 3714
Size: 988 281 301 747 125 310 47 246
Cuts at: 3714 669
Size: 3079
Fragments arranged by size:
3079 988 747 310 301 281 246 125
47
HindIII A'AGCT_T
Cuts at: 748 3026 748
Size: 2278 3846
HpaI GTT'AAC
Cuts at: 3421 3714 3421
Size: 293 5831
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4302
Fragments arranged by size:
4302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2439 2849 2924 4082 4441 4600
Size: 648 1056 217 410 75 1158 359 159
Cuts at: 4600 518
Size: 2042
Fragments arranged by size:
2042 1158 1056 648 410 359 217 159
75
MspA1I CmG'CkG
Cuts at: 1606 3540 4217 5365 5610 1606
Size: 1934 677 1148 245 2120
Fragments arranged by size:
2120 1934 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NsiI A_TGCA'T
Cuts at: 2489 2818 2489
Size: 329 5795
NspI r_CATG'y
Cuts at: 1132 1229 2107 2487 2929 3226 3482 5027 1132
Size: 97 878 380 442 297 256 1545 2229
Fragments arranged by size:
2229 1545 878 442 380 297 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 2103 2925 5023 2103
Size: 822 2098 3204
Fragments arranged by size:
3204 2098 822
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 3359 3359
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2177 2177
Size: 6124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 6092
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SacI G_AGCT'C
Cuts at: 2628 2628
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SanDI GG'GwC_CC
Cuts at: 2177 2177
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 3472 4607 5288 5479 826
Size: 219 2427 1135 681 191 1471
Fragments arranged by size:
2427 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 2098 3451 1872
Size: 226 1353 4545
Fragments arranged by size:
4545 1353 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2662 3228 4228 5129 5391 820
Size: 197 926 719 566 1000 901 262 1553
Fragments arranged by size:
1553 1000 926 901 719 566 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 2487 3226 3482 1132
Size: 1355 739 256 3774
Fragments arranged by size:
3774 1355 739 256
SspI AAT'ATT
Cuts at: 5 52 2276 3103 4048 5
Size: 47 2224 827 945 2081
Fragments arranged by size:
2224 2081 945 827 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 3045 3563 513
Size: 552 1980 518 3074
Fragments arranged by size:
3074 1980 552 518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2779 2891 4151 4168 4321 4506
Size: 1255 764 435 112 1260 17 153 185
Cuts at: 4506 4951 325
Size: 445 1498
Fragments arranged by size:
1498 1260 1255 764 445 435 185 153
112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3207 3411 4370
Size: 274 80 33 51 1443 1230 204 959
Cuts at: 4370 6082 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1230 959 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 6084 6116 2394
Size: 32 3658 32 2402
Fragments arranged by size:
3658 2402 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2221
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 2662 3228 1943
Size: 719 566 4839
Fragments arranged by size:
4839 719 566
XmaI C'CCGG_G
Cuts at: 1870 2096 3449 1870
Size: 226 1353 4545
Fragments arranged by size:
4545 1353 226
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PvuII RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI