Restriction map for
pFN21AB4329
(Circular) (Six-base) MAPSORT of: pFN21AB4329.seq Check: 3396 from: 1 to: 7516
pFN21AB4329 7516 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3800 4060 3800
Size: 260 7256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5326 278
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 4794 4859 1937
Size: 412 2445 65 4594
Fragments arranged by size:
4594 2445 412 65
Acc65I G'GTAC_C
Cuts at: 4837 4837
Size: 7516
AclI AA'CG_TT
Cuts at: 1563 4815 5643 6016 1563
Size: 3252 828 373 3063
Fragments arranged by size:
3252 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 4435 4538 4697 4772 4788 5579 6963
Size: 1235 1568 103 159 75 16 791 1384
Cuts at: 6963 7231 1632
Size: 268 1917
Fragments arranged by size:
1917 1568 1384 1235 791 268 159 103
75 16
AfeI AGC'GCT
Cuts at: 3173 3173
Size: 7516
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7319
AflIII A'CryG_T
Cuts at: 1203 6415 7392 1203
Size: 5212 977 1327
Fragments arranged by size:
5212 1327 977
AgeI A'CCGG_T
Cuts at: 6347 6347
Size: 7516
AhdI GACnn_n'nnGTC
Cuts at: 2680 6245 2680
Size: 3565 3951
AleI CACnn'nnGTG
Cuts at: 1166 3440 4753 1166
Size: 2274 1313 3929
Fragments arranged by size:
3929 2274 1313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2449 3458 1184
Size: 1265 1009 5242
Fragments arranged by size:
5242 1265 1009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4701 4733 4701
Size: 32 7484
AlwNI CAG_nnn'CTG
Cuts at: 3067 5259 6831 3067
Size: 2192 1572 3752
Fragments arranged by size:
3752 2192 1572
ApaI G_GGCC'C
Cuts at: 4382 4382
Size: 7516
ApaLI G'TGCA_C
Cuts at: 5573 6729 5573
Size: 1156 6360
ApoI r'AATT_y
Cuts at: 1493 3765 4825 5051 5203 7312 1493
Size: 2272 1060 226 152 2109 1697
Fragments arranged by size:
2272 2109 1697 1060 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2616 6070 160
Size: 872 1584 3454 1606
Fragments arranged by size:
3454 1606 1584 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 3336 4841 1870
Size: 73 1393 1505 4545
Fragments arranged by size:
4545 1505 1393 73
AvrII C'CTAG_G
Cuts at: 3970 3970
Size: 7516
BamHI G'GATC_C
Cuts at: 2404 4846 5213 2404
Size: 2442 367 4707
Fragments arranged by size:
4707 2442 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3585 4111 4837 6292 618
Size: 325 217 627 1798 526 726 1455 1842
Fragments arranged by size:
1842 1798 1455 726 627 526 325 217
BanII G_rGCy'C
Cuts at: 1391 4382 4765 4835 1391
Size: 2991 383 70 4072
Fragments arranged by size:
4072 2991 383 70
BbeI G_GCGC'C
Cuts at: 3589 3589
Size: 7516
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2965 4435 4504 928
Size: 971 1066 1470 69 3940
Fragments arranged by size:
3940 1470 1066 971 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5707 5741 1903
Size: 34 3770 34 3678
Fragments arranged by size:
3770 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4727 5408 6618 4727
Size: 681 1210 5625
Fragments arranged by size:
5625 1210 681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 2549 3825
Size: 107 122 71 631 605 589 288 1276
Cuts at: 3825 6127 136
Size: 2302 1525
Fragments arranged by size:
2302 1525 1276 631 605 589 288 122
107 71
BglII A'GATC_T
Cuts at: 7511 7511
Size: 7516
BlpI GC'TnA_GC
Cuts at: 4933 4933
Size: 7516
Bme1580I G_kGCm'C
Cuts at: 1790 4209 4382 5577 6733 1790
Size: 2419 173 1195 1156 2573
Fragments arranged by size:
2573 2419 1195 1156 173
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7516
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3914 4376 5247
Size: 416 53 83 399 24 2497 462 871
Cuts at: 5247 6205 442
Size: 958 1753
Fragments arranged by size:
2497 1753 958 871 462 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3434 4397 6176 1239
Size: 2195 963 1779 2579
Fragments arranged by size:
2579 2195 1779 963
Bpu10I CC'TnA_GC
Cuts at: 3060 3060
Size: 7516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3030 3467 3544 3858 5641 6506 6804 3030
Size: 437 77 314 1783 865 298 3742
Fragments arranged by size:
3742 1783 865 437 314 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3558 6179 882
Size: 617 2059 2621 2219
Fragments arranged by size:
2621 2219 2059 617
BsaAI yAC'GTr
Cuts at: 493 1204 4715 5307 493
Size: 711 3511 592 2702
Fragments arranged by size:
3511 2702 711 592
BsaBI GATnn'nnATC
Cuts at: 2298 2403 2520 2666 7510 2298
Size: 105 117 146 4844 2304
Fragments arranged by size:
4844 2304 146 117 105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 5323 5705 6340
Size: 53 83 186 936 2053 1737 382 635
Cuts at: 6340 275
Size: 1451
Fragments arranged by size:
2053 1737 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4181 5949 6347 6621 6768 7179 4181
Size: 1768 398 274 147 411 4518
Fragments arranged by size:
4518 1768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4021 4051 1178
Size: 30 165 30 2618 30 4643
Fragments arranged by size:
4643 2618 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3668 3990 4299 1186
Size: 2482 322 309 4403
Fragments arranged by size:
4403 2482 322 309
BseYI C'CCAG_C
Cuts at: 4301 6719 7215 4301
Size: 2418 496 4602
Fragments arranged by size:
4602 2418 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2918 3464 1163
Size: 573 1182 546 5215
Fragments arranged by size:
5215 1182 573 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4966 5727 6755 1540
Size: 275 186 2965 761 1028 2301
Fragments arranged by size:
2965 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3803 4765 5577 5662 6733 3803
Size: 962 812 85 1071 4586
Fragments arranged by size:
4586 1071 962 812 85
BsmI GAATG_Cn'
Cuts at: 2648 5027 5120 2648
Size: 2379 93 5044
Fragments arranged by size:
5044 2379 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3650 3650
Size: 7516
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3803 4209 4382 4765 4835 5577 5662
Size: 399 2013 406 173 383 70 742 85
Cuts at: 5662 6733 1391
Size: 1071 2174
Fragments arranged by size:
2174 2013 1071 742 406 399 383 173
85 70
BspEI T'CCGG_A
Cuts at: 4181 7179 4181
Size: 2998 4518
BspHI T'CATG_A
Cuts at: 3027 4386 5405 7135 3027
Size: 1359 1019 1730 3408
Fragments arranged by size:
3408 1730 1359 1019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3508 3800 4060 4159 4871 844
Size: 2664 292 260 99 712 3489
Fragments arranged by size:
3489 2664 712 292 260 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2380 2731 5403 1394
Size: 429 557 351 2672 3507
Fragments arranged by size:
3507 2672 557 429 351
BsrDI GCAATG_nn'
Cuts at: 65 1248 3054 3185 6011 6185 65
Size: 1183 1806 131 2826 174 1396
Fragments arranged by size:
2826 1806 1396 1183 174 131
BsrFI r'CCGG_y
Cuts at: 1700 1810 6160 6347 1700
Size: 110 4350 187 2869
Fragments arranged by size:
4350 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7516
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7516
BssSI C'ACGA_G
Cuts at: 5576 6588 5576
Size: 1012 6504
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4034 4660 794
Size: 3240 626 3650
Fragments arranged by size:
3650 3240 626
BstBI TT'CG_AA
Cuts at: 3115 6320 3115
Size: 3205 4311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7516
BstXI CCAn_nnnn'nTGG
Cuts at: 3154 3952 3154
Size: 798 6718
BstYI r'GATC_y
Cuts at: 1972 2293 2404 2503 3892 3964 4846 5213 5598
Size: 321 111 99 1389 72 882 367 385
Cuts at: 5598 5615 7067 7153 7165 7511 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1389 882 385 367 346 321
111 99 86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2817 2912 3858 4134 2817
Size: 95 946 276 6199
Fragments arranged by size:
6199 946 276 95
BtgI C'CryG_G
Cuts at: 513 1065 2130 2331 513
Size: 552 1065 201 5698
Fragments arranged by size:
5698 1065 552 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3274 487
Size: 683 56 2048 4729
Fragments arranged by size:
4729 2048 683 56
BtsI GCAGTG_nn'
Cuts at: 2772 3834 3966 4072 5032 5826 5846 2772
Size: 1062 132 106 960 794 20 4442
Fragments arranged by size:
4442 1062 960 794 132 106 20
ClaI AT'CG_AT
Cuts at: 1554 4437 1554
Size: 2883 4633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2514 2549 529
Size: 35 1950 35 5496
Fragments arranged by size:
5496 1950 35 35
DraI TTT'AAA
Cuts at: 3900 4581 4820 3900
Size: 681 239 6596
Fragments arranged by size:
6596 681 239
DraIII CAC_nnn'GTG
Cuts at: 3300 4118 3300
Size: 818 6698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3987 6523 809
Size: 507 2671 2536 1802
Fragments arranged by size:
2671 2536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 4314 4704
Size: 54 1425 50 275 18 423 2061 390
Cuts at: 4704 4963 5852 7481 8
Size: 259 889 1629 43
Fragments arranged by size:
2061 1629 1425 889 423 390 275 259
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4963 1537
Size: 275 3151 4090
Fragments arranged by size:
4090 3151 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5446 1353
Size: 4093 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6103 6477 6623 387
Size: 1064 138 4514 374 146 1280
Fragments arranged by size:
4514 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4763 4763
Size: 7516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2867 3434 4397 4435 4538 4697 4772
Size: 393 1235 567 963 38 103 159 75
Cuts at: 4772 4788 5579 6176 6963 7231 1239
Size: 16 791 597 787 268 1524
Fragments arranged by size:
1524 1235 963 791 787 597 567 393
268 159 103 75 38 16
EcoRI G'AATT_C
Cuts at: 3765 4825 5203 3765
Size: 1060 378 6078
Fragments arranged by size:
6078 1060 378
EcoRV GAT'ATC
Cuts at: 2455 2455
Size: 7516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3899 3899
Size: 7516
FspI TGC'GCA
Cuts at: 6022 6022
Size: 7516
HaeII r_GCGC'y
Cuts at: 1678 3175 3589 4507 6663 1678
Size: 1497 414 918 2156 2531
Fragments arranged by size:
2531 2156 1497 918 414
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 2666 2698 4384
Size: 32 135 32 601 32 461 32 1686
Cuts at: 4384 4416 6157 6189 6231 6263 1373
Size: 32 1741 32 42 32 2626
Fragments arranged by size:
2626 1741 1686 601 461 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 4860 5106 669
Size: 988 281 1168 1754 246 3079
Fragments arranged by size:
3079 1754 1168 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2204 3406 748
Size: 1456 1202 4858
Fragments arranged by size:
4858 1456 1202
HpaI GTT'AAC
Cuts at: 5106 5106
Size: 7516
KasI G'GCGC_C
Cuts at: 3585 3585
Size: 7516
KpnI G_GTAC'C
Cuts at: 4841 4841
Size: 7516
MfeI C'AATT_G
Cuts at: 3234 5115 3234
Size: 1881 5635
MluI A'CGCG_T
Cuts at: 7392 7392
Size: 7516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3000 6630 6814 1305
Size: 1695 3630 184 2007
Fragments arranged by size:
3630 2007 1695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 4316 10
Size: 54 1425 343 423 2061 3210
Fragments arranged by size:
3210 2061 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3152 3440 3950 4214 4391 4581 4753
Size: 648 1986 288 510 264 177 190 172
Cuts at: 4753 5474 5833 5992 518
Size: 721 359 159 2042
Fragments arranged by size:
2042 1986 721 648 510 359 288 264
190 177 172 159
MspA1I CmG'CkG
Cuts at: 1606 2780 4455 4932 5609 6757 7002 1606
Size: 1174 1675 477 677 1148 245 2120
Fragments arranged by size:
2120 1675 1174 1148 677 477 245
NarI GG'CG_CC
Cuts at: 3586 3586
Size: 7516
NcoI C'CATG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 5698
Fragments arranged by size:
5698 1266 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7516
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NspI r_CATG'y
Cuts at: 1132 1229 4663 4874 6419 1132
Size: 97 3434 211 1545 2229
Fragments arranged by size:
3434 2229 1545 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7516
PciI A'CATG_T
Cuts at: 6415 6415
Size: 7516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 1108
Size: 1362 6154
PfoI T'CCnGG_A
Cuts at: 2670 3411 2670
Size: 741 6775
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4177 4209 5585 5617 7098 7130 3299
Size: 32 846 32 1376 32 1481 32 3685
Fragments arranged by size:
3685 1481 1376 846 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3912 1613
Size: 2299 5217
PsiI TTA'TAA
Cuts at: 5086 5086
Size: 7516
PspOMI G'GGCC_C
Cuts at: 4378 4378
Size: 7516
PspXI vC'TCGA_Gb
Cuts at: 3336 3336
Size: 7516
PstI C_TGCA'G
Cuts at: 830 3169 4156 4248 4868 830
Size: 2339 987 92 620 3478
Fragments arranged by size:
3478 2339 987 620 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 4455 4455
Size: 7516
SacI G_AGCT'C
Cuts at: 4765 4765
Size: 7516
SalI G'TCGA_C
Cuts at: 1936 4858 1936
Size: 2922 4594
SbfI CC_TGCA'GG
Cuts at: 4868 4868
Size: 7516
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SexAI A'CCwGG_T
Cuts at: 4114 4114
Size: 7516
SfcI C'TryA_G
Cuts at: 826 1045 3090 3165 4152 4244 4864 5999 6680
Size: 219 2045 75 987 92 620 1135 681
Cuts at: 6680 6871 826
Size: 191 1471
Fragments arranged by size:
2045 1471 1135 987 681 620 219 191
92 75
SfoI GGC'GCC
Cuts at: 3587 3587
Size: 7516
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7516
SmaI CCC'GGG
Cuts at: 1872 4843 1872
Size: 2971 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3045 3336 3482 3523 3873 5620
Size: 197 926 1102 291 146 41 350 1747
Cuts at: 5620 6521 6783 820
Size: 901 262 1553
Fragments arranged by size:
1747 1553 1102 926 901 350 291 262
197 146 41
SnaBI TAC'GTA
Cuts at: 493 4715 5307 493
Size: 4222 592 2702
Fragments arranged by size:
4222 2702 592
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7516
SphI G_CATG'C
Cuts at: 1132 4874 1132
Size: 3742 3774
SspI AAT'ATT
Cuts at: 5 52 2049 5440 5
Size: 47 1997 3391 2081
Fragments arranged by size:
3391 2081 1997 47
StuI AGG'CCT
Cuts at: 1517 2944 1517
Size: 1427 6089
StyI C'CwwG_G
Cuts at: 513 1065 2316 2331 3970 4201 4374 4955 513
Size: 552 1251 15 1639 231 173 581 3074
Fragments arranged by size:
3074 1639 1251 581 552 231 173 15
SwaI ATTT'AAAT
Cuts at: 4581 4581
Size: 7516
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2592 3452 3902 4009 5543 5560 5713
Size: 1255 1012 860 450 107 1534 17 153
Cuts at: 5713 5898 6343 325
Size: 185 445 1498
Fragments arranged by size:
1534 1498 1255 1012 860 450 445 185
153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 5762 7474
Size: 274 80 33 51 1443 1008 2777 1712
Cuts at: 7474 96
Size: 138
Fragments arranged by size:
2777 1712 1443 1008 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7476 7508 7476
Size: 32 7484
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7516
XbaI T'CTAG_A
Cuts at: 2803 4852 2803
Size: 2049 5467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2764 3745 4309 4642 2764
Size: 981 564 333 5638
Fragments arranged by size:
5638 981 564 333
XhoI C'TCGA_G
Cuts at: 1943 3336 1943
Size: 1393 6123
XmaI C'CCGG_G
Cuts at: 1870 4841 1870
Size: 2971 4545
XmnI GAAnn'nnTTC
Cuts at: 5645 5645
Size: 7516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5324 276
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoNI
EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PsrI RsrII SacII SanDI SapI SfiI
SrfI