Restriction map for
pFN21AB4325
(Circular) (Six-base) MAPSORT of: pFN21AB4325.seq Check: 5821 from: 1 to: 6013
pFN21AB4325 6013 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2615 2615
Size: 6013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 3356 1937
Size: 340 1079 4594
Fragments arranged by size:
4594 1079 340
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2268 2711 2942 3137 4076 5460 5728 1632
Size: 636 443 231 195 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 636 443 268 231 195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 2819 3128 1166
Size: 1653 309 4051
Fragments arranged by size:
4051 1653 309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 5981
AlwNI CAG_nnn'CTG
Cuts at: 2345 3160 3756 5328 2345
Size: 815 596 1572 3030
Fragments arranged by size:
3030 1572 815 596
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2287 2902 2952 3322 3548 3700 5809 1493
Size: 794 615 50 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 794 615 370 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 3338 1870
Size: 73 1395 4545
Fragments arranged by size:
4545 1395 73
BamHI G'GATC_C
Cuts at: 2709 3343 3710 2709
Size: 634 367 5012
Fragments arranged by size:
5012 634 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2575 3254 3334 4789
Size: 325 217 627 762 26 679 80 1455
Cuts at: 4789 618
Size: 1842
Fragments arranged by size:
1842 1455 762 679 627 325 217 80
26
BanII G_rGCy'C
Cuts at: 1391 2129 2246 3090 3332 1391
Size: 738 117 844 242 4072
Fragments arranged by size:
4072 844 738 242 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2953 928
Size: 971 1054 3988
Fragments arranged by size:
3988 1054 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2806 2840 4204 4238 1903
Size: 34 869 34 1364 34 3678
Fragments arranged by size:
3678 1364 869 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3182 4624
Size: 107 122 71 631 605 337 1173 1442
Cuts at: 4624 136
Size: 1525
Fragments arranged by size:
1525 1442 1173 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2746 6008 2746
Size: 3262 2751
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 3259 4074 5230 1790
Size: 1469 815 1156 2573
Fragments arranged by size:
2573 1469 1156 815
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4673 1239
Size: 3434 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 4138 5003 5301 2414
Size: 1724 865 298 3126
Fragments arranged by size:
3126 1724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2981 4676 882
Size: 617 1482 1695 2219
Fragments arranged by size:
2219 1695 1482 617
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2582 2796 3820 4202
Size: 53 83 186 936 1049 214 1024 382
Cuts at: 4202 4837 275
Size: 635 1451
Fragments arranged by size:
1451 1049 1024 936 635 382 214 186
83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3096 3126 3287 3317 1178
Size: 30 165 30 1693 30 161 30 3874
Fragments arranged by size:
3874 1693 165 161 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3257 1186
Size: 2071 3942
BseYI C'CCAG_C
Cuts at: 2523 2721 5216 5712 2523
Size: 198 2495 496 2824
Fragments arranged by size:
2824 2495 496 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 3209 1163
Size: 573 801 672 3967
Fragments arranged by size:
3967 801 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2655 3463 4224 5252 1540
Size: 275 186 654 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 654 275 186
BsiHKAI G_wGCw'C
Cuts at: 3090 4074 4159 5230 3090
Size: 984 85 1071 3873
Fragments arranged by size:
3873 1071 984 85
BsmI GAATG_Cn'
Cuts at: 2382 3524 3617 2382
Size: 1142 93 4778
Fragments arranged by size:
4778 1142 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2246 3090 3259 3332 4074 4159
Size: 399 339 117 844 169 73 742 85
Cuts at: 4159 5230 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 844 742 399 339 169 117
85 73
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3198 3902 5632 3198
Size: 704 1730 3579
Fragments arranged by size:
3579 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2615 3184 3301 3368 844
Size: 1771 569 117 67 3489
Fragments arranged by size:
3489 1771 569 117 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2442 4508 4682 65
Size: 1183 1194 2066 174 1396
Fragments arranged by size:
2066 1396 1194 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2578 3021 4657 4844 1700
Size: 110 768 443 1636 187 2869
Fragments arranged by size:
2869 1636 768 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2056 794
Size: 1262 4751
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2089 2335 2709 2746 3343 3710 4095 4112
Size: 117 246 374 37 597 367 385 17
Cuts at: 4112 5564 5650 5662 6008 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 597 385 374 367 346 246
117 86 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2562 2562
Size: 6013
BtgI C'CryG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 3916
Fragments arranged by size:
3916 1545 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 4323 4343 3529
Size: 794 20 5199
Fragments arranged by size:
5199 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3023 3460 4349
Size: 54 1425 50 275 18 1193 437 889
Cuts at: 4349 5978 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1193 889 437 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2843 3943 1353
Size: 1490 1100 3423
Fragments arranged by size:
3423 1490 1100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3088 3088
Size: 6013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2268 2711 2942 3137 4076 4673 5460
Size: 393 636 443 231 195 939 597 787
Cuts at: 5460 5728 1239
Size: 268 1524
Fragments arranged by size:
1524 939 787 636 597 443 393 268
231 195
EcoRI G'AATT_C
Cuts at: 2952 3322 3700 2952
Size: 370 378 5265
Fragments arranged by size:
5265 378 370
FspI TGC'GCA
Cuts at: 2381 4519 2381
Size: 2138 3875
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 2878 2910 3096
Size: 32 135 32 1190 32 84 32 186
Cuts at: 3096 3128 3209 3241 4654 4686 4728 4760 1373
Size: 32 81 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 1190 186 135 84 81 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 2923 3357 3603 669
Size: 988 281 472 513 434 246 3079
Fragments arranged by size:
3079 988 513 472 434 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 2410 3603 2410
Size: 1193 4820
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2949 3078 5127 5311 1305
Size: 1644 129 2049 184 2007
Fragments arranged by size:
2049 2007 1644 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 2819 3128 3140 3971 4330 4489
Size: 648 1600 53 309 12 831 359 159
Cuts at: 4489 518
Size: 2042
Fragments arranged by size:
2042 1600 831 648 359 309 159 53
12
MspA1I CmG'CkG
Cuts at: 1606 3279 3429 4106 5254 5499 1606
Size: 1673 150 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 677 245 150
NaeI GCC'GGC
Cuts at: 2580 2580
Size: 6013
NcoI C'CATG_G
Cuts at: 513 1065 2610 513
Size: 552 1545 3916
Fragments arranged by size:
3916 1545 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2578 2578
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2813 3371 4916 1132
Size: 97 1584 558 1545 2229
Fragments arranged by size:
2229 1584 1545 558 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 4082 4114 5595 5627 3287
Size: 32 763 32 1481 32 3673
Fragments arranged by size:
3673 1481 763 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PstI C_TGCA'G
Cuts at: 830 2435 2595 3312 3365 830
Size: 1605 160 717 53 3478
Fragments arranged by size:
3478 1605 717 160 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SacI G_AGCT'C
Cuts at: 3090 3090
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SapI GCTCTTCn'nnn_
Cuts at: 2843 2843
Size: 6013
SbfI CC_TGCA'GG
Cuts at: 3312 3365 3312
Size: 53 5960
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2431 2591 3308 3361 4496 5177 5368
Size: 219 1386 160 717 53 1135 681 191
Cuts at: 5368 826
Size: 1471
Fragments arranged by size:
1471 1386 1135 717 681 219 191 160
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 4117 5018 5280 820
Size: 197 926 450 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 2813 3371 1132
Size: 1681 558 3774
Fragments arranged by size:
3774 1681 558
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2610 2665 3452 513
Size: 552 1545 55 787 3074
Fragments arranged by size:
3074 1545 787 552 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4040 4057 4210 4395 4840 325
Size: 1255 2460 17 153 185 445 1498
Fragments arranged by size:
2460 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 5973 6005 2559
Size: 32 3382 32 2567
Fragments arranged by size:
3382 2567 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2689 1316
Size: 1373 4640
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2617 2617
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2321 4142 2321
Size: 1821 4192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI