Restriction map for
pFN21AB4321
(Circular) (Six-base) MAPSORT of: pFN21AB4321.seq Check: 1193 from: 1 to: 7717
pFN21AB4321 7717 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3200 4046 3200
Size: 846 6871
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5527 278
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2688 4952 5060 1937
Size: 751 2264 108 4594
Fragments arranged by size:
4594 2264 751 108
Acc65I G'GTAC_C
Cuts at: 3243 5038 3243
Size: 1795 5922
AclI AA'CG_TT
Cuts at: 1563 5844 6217 1563
Size: 4281 373 3063
Fragments arranged by size:
4281 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3588 3640 4853 5780 7164 7432 1632
Size: 1956 52 1213 927 1384 268 1917
Fragments arranged by size:
1956 1917 1384 1213 927 268 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7520
AflIII A'CryG_T
Cuts at: 1203 3168 4797 6616 7593 1203
Size: 1965 1629 1819 977 1327
Fragments arranged by size:
1965 1819 1629 1327 977
AgeI A'CCGG_T
Cuts at: 6548 6548
Size: 7717
AhdI GACnn_n'nnGTC
Cuts at: 6446 6446
Size: 7717
AleI CACnn'nnGTG
Cuts at: 1166 3425 3686 1166
Size: 2259 261 5197
Fragments arranged by size:
5197 2259 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7717
AlwNI CAG_nnn'CTG
Cuts at: 5460 7032 5460
Size: 1572 6145
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 7717
ApaLI G'TGCA_C
Cuts at: 5774 6930 5774
Size: 1156 6561
ApoI r'AATT_y
Cuts at: 1493 2188 2396 3621 4001 4061 4524 5026 5252
Size: 695 208 1225 380 60 463 502 226
Cuts at: 5252 5404 7513 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1225 695 502 463 380 226
208 152 60
AseI AT'TA_AT
Cuts at: 160 1032 6271 160
Size: 872 5239 1606
Fragments arranged by size:
5239 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2022 4473 4493 5042 1870
Size: 73 71 8 2451 20 549 4545
Fragments arranged by size:
4545 2451 549 73 71 20 8
AvrII C'CTAG_G
Cuts at: 4171 4171
Size: 7717
BamHI G'GATC_C
Cuts at: 3979 5047 5414 3979
Size: 1068 367 6282
Fragments arranged by size:
6282 1068 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2854 2903 3243 5038 6493
Size: 325 217 627 1067 49 340 1795 1455
Cuts at: 6493 618
Size: 1842
Fragments arranged by size:
1842 1795 1455 1067 627 340 325 217
49
BanII G_rGCy'C
Cuts at: 1391 2015 2022 2119 3359 3367 5036 1391
Size: 624 7 97 1240 8 1669 4072
Fragments arranged by size:
4072 1669 1240 624 97 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2722 3607 3646 4841 928
Size: 971 823 885 39 1195 3804
Fragments arranged by size:
3804 1195 971 885 823 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5908 5942 1903
Size: 34 50 34 3887 34 3678
Fragments arranged by size:
3887 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4970 5609 6819 4970
Size: 639 1210 5868
Fragments arranged by size:
5868 1210 639
BclI T'GATC_A
Cuts at: 1548 2482 4201 1548
Size: 934 1719 5064
Fragments arranged by size:
5064 1719 934
BfrBI ATG'CAT
Cuts at: 2871 3913 4850 2871
Size: 1042 937 5738
Fragments arranged by size:
5738 1042 937
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6328 136
Size: 107 122 71 631 605 4656 1525
Fragments arranged by size:
4656 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4405 7712 4405
Size: 3307 4410
BlpI GC'TnA_GC
Cuts at: 5134 5134
Size: 7717
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2859 3930 5778 6934 1790
Size: 232 837 1071 1848 1156 2573
Fragments arranged by size:
2573 1848 1156 1071 837 232
BmgBI CAC'GTC
Cuts at: 1318 2130 3169 1318
Size: 812 1039 5866
Fragments arranged by size:
5866 1039 812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 3680 4654
Size: 416 53 83 399 24 1027 1236 974
Cuts at: 4654 5448 6406 442
Size: 794 958 1753
Fragments arranged by size:
1753 1236 1027 974 958 794 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4300 4300
Size: 7717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2448 2769 3399 4612 4920 6377 1239
Size: 1209 321 630 1213 308 1457 2579
Fragments arranged by size:
2579 1457 1213 1209 630 321 308
Bpu10I CC'TnA_GC
Cuts at: 4008 5001 4008
Size: 993 6724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 5842 6707 7005 3373
Size: 2469 865 298 4085
Fragments arranged by size:
4085 2469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3533 4630 6380 882
Size: 617 2034 1097 1750 2219
Fragments arranged by size:
2219 2034 1750 1097 617
BsaAI yAC'GTr
Cuts at: 493 1204 3632 4817 5508 493
Size: 711 2428 1185 691 2702
Fragments arranged by size:
2702 2428 1185 711 691
BsaBI GATnn'nnATC
Cuts at: 3984 7711 3984
Size: 3727 3990
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2035 3780 5524 5906
Size: 53 83 186 936 502 1745 1744 382
Cuts at: 5906 6541 275
Size: 635 1451
Fragments arranged by size:
1745 1744 1451 936 635 502 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2154 6150 6548 6822 6969 7380 2154
Size: 3996 398 274 147 411 2491
Fragments arranged by size:
3996 2491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7492
Fragments arranged by size:
7492 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3075 3236 3776 1186
Size: 1889 161 540 5127
Fragments arranged by size:
5127 1889 540 161
BseYI C'CCAG_C
Cuts at: 2077 2174 6920 7416 2077
Size: 97 4746 496 2378
Fragments arranged by size:
4746 2378 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 2938 1163
Size: 573 352 850 5942
Fragments arranged by size:
5942 850 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5167 5928 6956 1540
Size: 275 186 3166 761 1028 2301
Fragments arranged by size:
3166 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3631 5778 5863 6934 3631
Size: 2147 85 1071 4414
Fragments arranged by size:
4414 2147 1071 85
BsmI GAATG_Cn'
Cuts at: 3097 5228 5321 3097
Size: 2131 93 5493
Fragments arranged by size:
5493 2131 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2022 2119 2859 3359 3367 3631
Size: 399 225 7 97 740 500 8 264
Cuts at: 3631 3930 5036 5778 5863 6934 1391
Size: 299 1106 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 742 740 500 399 299
264 225 97 85 8 7
BspEI T'CCGG_A
Cuts at: 2154 7380 2154
Size: 5226 2491
BspHI T'CATG_A
Cuts at: 3282 5606 7336 3282
Size: 2324 1730 3663
Fragments arranged by size:
3663 2324 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3200 4046 5072 844
Size: 2356 846 1026 3489
Fragments arranged by size:
3489 2356 1026 846
BsrBI CCG'CTC
Cuts at: 1394 1823 5604 1394
Size: 429 3781 3507
Fragments arranged by size:
3781 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 2532 2664 6212 6386 65
Size: 1183 937 347 132 3548 174 1396
Fragments arranged by size:
3548 1396 1183 937 347 174 132
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2090 4947 6361 6548 1700
Size: 110 227 53 2857 1414 187 2869
Fragments arranged by size:
2869 2857 1414 227 187 110 53
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7717
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7717
BssSI C'ACGA_G
Cuts at: 5777 6789 5777
Size: 1012 6705
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3195 794
Size: 2401 5316
BstBI TT'CG_AA
Cuts at: 6521 6521
Size: 7717
BstEII G'GTnAC_C
Cuts at: 1184 2570 4234 5005 1184
Size: 1386 1664 771 3896
Fragments arranged by size:
3896 1664 1386 771
BstXI CCAn_nnnn'nTGG
Cuts at: 4536 4536
Size: 7717
BstYI r'GATC_y
Cuts at: 1972 2355 3292 3892 3979 4405 4895 5047 5414
Size: 383 937 600 87 426 490 152 367
Cuts at: 5414 5799 5816 7268 7354 7366 7712 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 937 600 490 426 385 383
367 346 152 87 86 17 12
BstZ17I GTA'TAC
Cuts at: 2689 4953 2689
Size: 2264 5453
BtgI C'CryG_G
Cuts at: 513 1065 4707 513
Size: 552 3642 3523
Fragments arranged by size:
3642 3523 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6978
Fragments arranged by size:
6978 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3339 3774 5233 6027 6047 2827
Size: 512 435 1459 794 20 4497
Fragments arranged by size:
4497 1459 794 512 435 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7717
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7682
DraI TTT'AAA
Cuts at: 5021 5021
Size: 7717
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6724 809
Size: 507 5408 1802
Fragments arranged by size:
5408 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2166 5164
Size: 54 1425 50 275 18 258 78 2998
Cuts at: 5164 6053 7682 8
Size: 889 1629 43
Fragments arranged by size:
2998 1629 1425 889 275 258 78 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5164 1537
Size: 275 3352 4090
Fragments arranged by size:
4090 3352 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2886 3894 5647 1353
Size: 897 636 1008 1753 3423
Fragments arranged by size:
3423 1753 1008 897 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3129 6304 6678 6824 387
Size: 1064 138 1540 3175 374 146 1280
Fragments arranged by size:
3175 1540 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2448 2769 3399 3588 3640 4612 4853
Size: 393 816 321 630 189 52 972 241
Cuts at: 4853 4920 5780 6377 7164 7432 1239
Size: 67 860 597 787 268 1524
Fragments arranged by size:
1524 972 860 816 787 630 597 393
321 268 241 189 67 52
EcoO109I rG'GnC_Cy
Cuts at: 2018 2019 2218 2439 2612 2018
Size: 1 199 221 173 7123
Fragments arranged by size:
7123 221 199 173 1
EcoRI G'AATT_C
Cuts at: 5026 5404 5026
Size: 378 7339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 7717
FspI TGC'GCA
Cuts at: 6223 6223
Size: 7717
HaeII r_GCGC'y
Cuts at: 1678 2838 6864 1678
Size: 1160 4026 2531
Fragments arranged by size:
4026 2531 1160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3277 3309 4642 4674 6358
Size: 32 135 32 1705 32 1333 32 1684
Cuts at: 6358 6390 6432 6464 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1705 1684 1333 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2794 4795 5061 5307 669
Size: 988 281 856 2001 266 246 3079
Fragments arranged by size:
3079 2001 988 856 281 266 246
HindIII A'AGCT_T
Cuts at: 748 2548 2997 748
Size: 1800 449 5468
Fragments arranged by size:
5468 1800 449
HpaI GTT'AAC
Cuts at: 5307 5307
Size: 7717
KpnI G_GTAC'C
Cuts at: 3247 5042 3247
Size: 1795 5922
MfeI C'AATT_G
Cuts at: 2765 5316 2765
Size: 2551 5166
MluI A'CGCG_T
Cuts at: 7593 7593
Size: 7717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2333 3372 4782 6831 7015 1305
Size: 1028 1039 1410 2049 184 2007
Fragments arranged by size:
2049 2007 1410 1039 1028 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 5559
Fragments arranged by size:
5559 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 3210 3287 3425 3686 3972 5675
Size: 648 1044 1000 77 138 261 286 1703
Cuts at: 5675 6034 6193 518
Size: 359 159 2042
Fragments arranged by size:
2042 1703 1044 1000 648 359 286 261
159 138 77
MspA1I CmG'CkG
Cuts at: 1606 5133 5810 6958 7203 1606
Size: 3527 677 1148 245 2120
Fragments arranged by size:
3527 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2039 2092 2039
Size: 53 7664
NcoI C'CATG_G
Cuts at: 513 1065 4707 513
Size: 552 3642 3523
Fragments arranged by size:
3642 3523 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7717
NgoMIV G'CCGG_C
Cuts at: 2037 2090 2037
Size: 53 7664
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7717
NotI GC'GGCC_GC
Cuts at: 5164 5164
Size: 7717
NsiI A_TGCA'T
Cuts at: 2873 3915 4852 2873
Size: 1042 937 5738
Fragments arranged by size:
5738 1042 937
NspI r_CATG'y
Cuts at: 1132 1229 2875 3066 3189 4348 4801 5075 6620
Size: 97 1646 191 123 1159 453 274 1545
Cuts at: 6620 1132
Size: 2229
Fragments arranged by size:
2229 1646 1545 1159 453 274 191 123
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7717
PciI A'CATG_T
Cuts at: 4797 6616 4797
Size: 1819 5898
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2246 1108
Size: 1138 6579
PfoI T'CCnGG_A
Cuts at: 2425 2425
Size: 7717
PmeI GTTT'AAAC
Cuts at: 5021 5021
Size: 7717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5786 5818 7299 7331 5786
Size: 32 1481 32 6172
Fragments arranged by size:
6172 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2439 2612 2218
Size: 221 173 7323
Fragments arranged by size:
7323 221 173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7717
PsiI TTA'TAA
Cuts at: 5287 5287
Size: 7717
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 7717
PstI C_TGCA'G
Cuts at: 830 2834 3586 4366 4859 5069 830
Size: 2004 752 780 493 210 3478
Fragments arranged by size:
3478 2004 780 752 493 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7717
SalI G'TCGA_C
Cuts at: 1936 5059 1936
Size: 3123 4594
SbfI CC_TGCA'GG
Cuts at: 5069 5069
Size: 7717
ScaI AGT'ACT
Cuts at: 5965 7677 5965
Size: 1712 6005
SfcI C'TryA_G
Cuts at: 826 1045 2830 3582 3874 4362 4855 4906 5065
Size: 219 1785 752 292 488 493 51 159
Cuts at: 5065 6200 6881 7072 826
Size: 1135 681 191 1471
Fragments arranged by size:
1785 1471 1135 752 681 493 488 292
219 191 159 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
SgrAI Cr'CCGG_yG
Cuts at: 1700 2037 1700
Size: 337 7380
SmaI CCC'GGG
Cuts at: 1872 2024 4475 5044 1872
Size: 152 2451 569 4545
Fragments arranged by size:
4545 2451 569 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3352 5821 6722 6984 820
Size: 197 926 1409 2469 901 262 1553
Fragments arranged by size:
2469 1553 1409 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4817 5508 493
Size: 4324 691 2702
Fragments arranged by size:
4324 2702 691
SpeI A'CTAG_T
Cuts at: 152 3116 152
Size: 2964 4753
SphI G_CATG'C
Cuts at: 1132 3066 3189 4348 5075 1132
Size: 1934 123 1159 727 3774
Fragments arranged by size:
3774 1934 1159 727 123
SspI AAT'ATT
Cuts at: 5 52 5641 5
Size: 47 5589 2081
Fragments arranged by size:
5589 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7717
StyI C'CwwG_G
Cuts at: 513 1065 4171 4707 5010 5156 513
Size: 552 3106 536 303 146 3074
Fragments arranged by size:
3106 3074 552 536 303 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 2822 3067 5744 5761 5914 6099
Size: 1255 658 584 245 2677 17 153 185
Cuts at: 6099 6544 325
Size: 445 1498
Fragments arranged by size:
2677 1498 1255 658 584 445 245 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5963 7675 96
Size: 274 80 33 51 1443 3986 1712 138
Fragments arranged by size:
3986 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2934 2966 7677 7709 2934
Size: 32 4711 32 2942
Fragments arranged by size:
4711 2942 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7717
XbaI T'CTAG_A
Cuts at: 5053 5053
Size: 7717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2247 3858 2247
Size: 1611 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7717
XmaI C'CCGG_G
Cuts at: 1870 2022 4473 5042 1870
Size: 152 2451 569 4545
Fragments arranged by size:
4545 2451 569 152
XmnI GAAnn'nnTTC
Cuts at: 3135 5846 3135
Size: 2711 5006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5525 276
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NarI NruI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI