Restriction map for
pFN21AB4307
(Circular) (Six-base) MAPSORT of: pFN21AB4307.seq Check: 6762 from: 1 to: 5707
pFN21AB4307 5707 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2544 3517 278
Size: 53 83 186 936 1008 973 2468
Fragments arranged by size:
2468 1008 973 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2046 2417 2896 3016 3242 3394 5503 1493
Size: 553 371 479 120 226 152 2109 1697
Fragments arranged by size:
2109 1697 553 479 371 226 152 120
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 2253 3037 3404 2253
Size: 784 367 4556
Fragments arranged by size:
4556 784 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2397 2691 3026 1391
Size: 1006 294 335 4072
Fragments arranged by size:
4072 1006 335 294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2395 2696 3599 4809 2395
Size: 301 903 1210 3293
Fragments arranged by size:
3293 1210 903 301
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2230 4318 136
Size: 107 122 71 631 605 558 2088 1525
Fragments arranged by size:
2088 1525 631 605 558 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 2226 3768 4924 1790
Size: 436 1542 1156 2573
Fragments arranged by size:
2573 1542 1156 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 3438 4396
Size: 416 53 83 399 24 772 1249 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1249 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 4367 1239
Size: 944 2184 2579
Fragments arranged by size:
2579 2184 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2221 3498 493
Size: 711 1017 1277 2702
Fragments arranged by size:
2702 1277 1017 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2541 3514 3896 4531
Size: 53 83 186 936 1008 973 382 635
Cuts at: 4531 275
Size: 1451
Fragments arranged by size:
1451 1008 973 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2834 1186
Size: 1648 4059
BseYI C'CCAG_C
Cuts at: 2225 2839 4910 5406 2225
Size: 614 2071 496 2526
Fragments arranged by size:
2526 2071 614 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2691 3768 3853 4924 2397
Size: 294 1077 85 1071 3180
Fragments arranged by size:
3180 1077 1071 294 85
BsiWI C'GTAC_G
Cuts at: 2193 2193
Size: 5707
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2532 2997 2532
Size: 465 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2397 2691 3026 3768 3853 4924
Size: 399 436 171 294 335 742 85 1071
Cuts at: 4924 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 436 399 335 294 171
85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 3062 844
Size: 1792 426 3489
Fragments arranged by size:
3489 1792 426
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2253 2382 3037 3404 3789 3806 5258 5344
Size: 281 129 655 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 655 385 367 346 281 129
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2811 487
Size: 683 56 1585 3383
Fragments arranged by size:
3383 1585 683 56
BtsI GCAGTG_nn'
Cuts at: 2250 3223 4017 4037 2250
Size: 973 794 20 3920
Fragments arranged by size:
3920 973 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2754 3154 4043
Size: 54 1425 50 275 18 924 400 889
Cuts at: 4043 5672 8
Size: 1629 43
Fragments arranged by size:
1629 1425 924 889 400 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2689 2395
Size: 294 5413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 3770 4367 5154 5422 1239
Size: 393 551 1587 597 787 268 1524
Fragments arranged by size:
1587 1524 787 597 551 393 268
EcoRI G'AATT_C
Cuts at: 2896 3016 3394 2896
Size: 120 378 5209
Fragments arranged by size:
5209 378 120
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2810 2837 2842
Size: 32 135 32 518 32 688 27 5
Cuts at: 2842 2861 2869 2893 2963 2995 4348 4380 4422
Size: 19 8 24 70 32 1353 32 42
Cuts at: 4422 4454 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 688 518 135 70 42 32
32 32 32 32 32 27 24 19
8 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 3051 3297 669
Size: 988 281 931 182 246 3079
Fragments arranged by size:
3079 988 931 281 246 182
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 2637 3306 2637
Size: 669 5038
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 2961
Fragments arranged by size:
2961 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2741 3665 4024 4183 518
Size: 648 1077 498 924 359 159 2042
Fragments arranged by size:
2042 1077 924 648 498 359 159
MspA1I CmG'CkG
Cuts at: 1606 2229 3123 3800 4948 5193 1606
Size: 623 894 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 623 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5155
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PmlI CAC'GTG
Cuts at: 2221 2221
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2860 3059 830
Size: 2030 199 3478
Fragments arranged by size:
3478 2030 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SacI G_AGCT'C
Cuts at: 2397 2691 2397
Size: 294 5413
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 2856 3055 4190 4871 5062 826
Size: 219 1811 199 1135 681 191 1471
Fragments arranged by size:
1811 1471 1135 681 219 199 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2109 3146 513
Size: 552 1044 1037 3074
Fragments arranged by size:
3074 1044 1037 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 3734 3751 3904 4089 4534 325
Size: 1255 984 1170 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1170 984 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 3953 5665
Size: 274 80 33 51 1443 580 1396 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1396 580 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2694 2726 2958 2990 5667 5699 2694
Size: 32 232 32 2677 32 2702
Fragments arranged by size:
2702 2677 232 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2542 3515 276
Size: 53 83 186 936 1008 973 2468
Fragments arranged by size:
2468 1008 973 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI