Restriction map for
pFN21AB4275
(Circular) (Six-base) MAPSORT of: pFN21AB4275.seq Check: 5934 from: 1 to: 6427
pFN21AB4275 6427 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2444 2980 2444
Size: 536 5891
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3346 3770 1937
Size: 1409 424 4594
Fragments arranged by size:
4594 1409 424
Acc65I G'GTAC_C
Cuts at: 3458 3748 3458
Size: 290 6137
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2649 4490 5874 6142 1632
Size: 941 76 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 941 268 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 2868 5326 6303 1203
Size: 1665 2458 977 1327
Fragments arranged by size:
2458 1665 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2988 3408 1184
Size: 1804 420 4203
Fragments arranged by size:
4203 1804 420
AlwNI CAG_nnn'CTG
Cuts at: 2633 3479 4170 5742 2633
Size: 846 691 1572 3318
Fragments arranged by size:
3318 1572 846 691
ApaI G_GGCC'C
Cuts at: 2984 2984
Size: 6427
ApaLI G'TGCA_C
Cuts at: 2186 4484 5640 2186
Size: 2298 1156 2973
Fragments arranged by size:
2973 2298 1156
ApoI r'AATT_y
Cuts at: 1493 2338 2723 3736 3962 4114 6223 1493
Size: 845 385 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 845 385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2394 3752 1870
Size: 73 168 283 1358 4545
Fragments arranged by size:
4545 1358 283 168 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3581 3614 3581
Size: 33 6394
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 2968 3458 3555 3680
Size: 325 217 627 596 585 490 97 125
Cuts at: 3680 3748 5203 618
Size: 68 1455 1842
Fragments arranged by size:
1842 1455 627 596 585 490 325 217
125 97 68
BanII G_rGCy'C
Cuts at: 1391 2522 2597 2984 3746 1391
Size: 1131 75 387 762 4072
Fragments arranged by size:
4072 1131 762 387 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 928
Size: 971 568 4888
Fragments arranged by size:
4888 971 568
BbvCI CC'TCA_GC
Cuts at: 2270 2682 2986 2270
Size: 412 304 5711
Fragments arranged by size:
5711 412 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4319 5529 3620
Size: 699 1210 4518
Fragments arranged by size:
4518 1210 699
BclI T'GATC_A
Cuts at: 1548 3720 1548
Size: 2172 4255
BfrBI ATG'CAT
Cuts at: 3100 3100
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 5038 136
Size: 107 122 71 631 605 427 2939 1525
Fragments arranged by size:
2939 1525 631 605 427 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2388 2627 2984 3447 3505 3558 4488
Size: 400 198 239 357 463 58 53 930
Cuts at: 4488 5644 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 930 463 400 357 239 198
58 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3721 4158 5116
Size: 416 53 83 399 24 2304 437 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
2304 1753 958 437 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2454 2813 5087 1239
Size: 996 219 359 2274 2579
Fragments arranged by size:
2579 2274 996 359 219
Bpu10I CC'TnA_GC
Cuts at: 2165 2270 2682 2986 2165
Size: 105 412 304 5606
Fragments arranged by size:
5606 412 304 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3435 4552 5417 5715 3435
Size: 1117 865 298 4147
Fragments arranged by size:
4147 1117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5090 882
Size: 617 3591 2219
Fragments arranged by size:
3591 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3442 4218 493
Size: 711 2238 776 2702
Fragments arranged by size:
2702 2238 776 711
BsaBI GATnn'nnATC
Cuts at: 2478 6421 2478
Size: 3943 2484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2376 4860 5258 5532 5679 6090 2376
Size: 2484 398 274 147 411 2713
Fragments arranged by size:
2713 2484 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6202
Fragments arranged by size:
6202 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2236 2800 2994 3704 1186
Size: 1050 564 194 710 3909
Fragments arranged by size:
3909 1050 710 564 194
BseYI C'CCAG_C
Cuts at: 2052 2102 2940 2949 3059 3165 3637 5630 6126
Size: 50 838 9 110 106 472 1993 496
Cuts at: 6126 2052
Size: 2353
Fragments arranged by size:
2353 1993 838 496 472 110 106 50
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2996 1163
Size: 573 1260 4594
Fragments arranged by size:
4594 1260 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 3004 3251 4488 4573 5644 2190
Size: 814 247 1237 85 1071 2973
Fragments arranged by size:
2973 1237 1071 814 247 85
BsmI GAATG_Cn'
Cuts at: 2323 3938 4031 2323
Size: 1615 93 4719
Fragments arranged by size:
4719 1615 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2388 2522 2597 2627 2984 3004
Size: 399 400 198 134 75 30 357 20
Cuts at: 3004 3251 3447 3505 3558 3746 4488 4573 5644
Size: 247 196 58 53 188 742 85 1071
Cuts at: 5644 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 400 399 357 247 198
196 188 134 85 75 58 53 30
20
BspEI T'CCGG_A
Cuts at: 2376 6090 2376
Size: 3714 2713
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 2980 3782 844
Size: 1600 536 802 3489
Fragments arranged by size:
3489 1600 802 536
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 4922 5096 65
Size: 1183 2142 1532 174 1396
Fragments arranged by size:
2142 1532 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5071 5258 1700
Size: 110 3261 187 2869
Fragments arranged by size:
3261 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 2139 1184
Size: 955 5472
BstYI r'GATC_y
Cuts at: 1972 2149 2289 2630 2752 3693 3757 4124 4509
Size: 177 140 341 122 941 64 367 385
Cuts at: 4509 4526 5978 6064 6076 6422 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 941 385 367 346 341 177
140 122 86 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2565 2565
Size: 6427
BtgI C'CryG_G
Cuts at: 513 1065 2004 2522 513
Size: 552 939 518 4418
Fragments arranged by size:
4418 939 552 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 2555 2655 3008 3943 4737 4757 2190
Size: 365 100 353 935 794 20 3860
Fragments arranged by size:
3860 935 794 365 353 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 2722 3267 3731 2722
Size: 545 464 5418
Fragments arranged by size:
5418 545 464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3093 3659 3874
Size: 54 1425 50 275 18 1263 566 215
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1263 889 566 275 215 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2809 4357 1353
Size: 1456 1548 3423
Fragments arranged by size:
3423 1548 1456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3080 5014 5388 5534 387
Size: 1064 138 1491 1934 374 146 1280
Fragments arranged by size:
1934 1491 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2454 2573 2649 2813 4490 5087
Size: 393 603 219 119 76 164 1677 597
Cuts at: 5087 5874 6142 1239
Size: 787 268 1524
Fragments arranged by size:
1677 1524 787 603 597 393 268 219
164 119 76
EcoO109I rG'GnC_Cy
Cuts at: 2687 2980 2981 2687
Size: 293 1 6133
Fragments arranged by size:
6133 293 1
EcoRI G'AATT_C
Cuts at: 2338 3736 4114 2338
Size: 1398 378 4651
Fragments arranged by size:
4651 1398 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 5574 1678
Size: 3896 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3233 3265 3274
Size: 32 135 32 896 32 733 32 9
Cuts at: 3274 3306 3328 3360 5068 5100 5142 5174 1373
Size: 32 22 32 1708 32 42 32 2626
Fragments arranged by size:
2626 1708 896 733 135 42 32 32
32 32 32 32 32 32 22 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2081 3274 748
Size: 1333 1193 3901
Fragments arranged by size:
3901 1333 1193
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3462 3752 3462
Size: 290 6137
MfeI C'AATT_G
Cuts at: 2427 3298 4026 2427
Size: 871 728 4828
Fragments arranged by size:
4828 871 728
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2312 5541 5725 1305
Size: 1007 3229 184 2007
Fragments arranged by size:
3229 2007 1007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3095 10
Size: 54 1425 343 1263 3342
Fragments arranged by size:
3342 1425 1263 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4385 4744 4903 518
Size: 648 3219 359 159 2042
Fragments arranged by size:
3219 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2286 2990 3112 3843 4520 5668 5913
Size: 450 230 704 122 731 677 1148 245
Cuts at: 5913 1606
Size: 2120
Fragments arranged by size:
2120 1148 731 704 677 450 245 230
122
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4936
Fragments arranged by size:
4936 939 552
NdeI CA'TA_TG
Cuts at: 387 3648 387
Size: 3261 3166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NsiI A_TGCA'T
Cuts at: 3102 3102
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2872 3785 5330 1132
Size: 97 1643 913 1545 2229
Fragments arranged by size:
2229 1643 1545 913 97
PasI CC'CwG_GG
Cuts at: 1348 2405 2977 1348
Size: 1057 572 4798
Fragments arranged by size:
4798 1057 572
PciI A'CATG_T
Cuts at: 2868 5326 2868
Size: 2458 3969
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2043 3677 1108
Size: 935 1634 3858
Fragments arranged by size:
3858 1634 935
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 3442 3442
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2687 2687
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 2980 2980
Size: 6427
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 6395
PstI C_TGCA'G
Cuts at: 830 3117 3779 830
Size: 2287 662 3478
Fragments arranged by size:
3478 2287 662
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2056 2990 2056
Size: 934 5493
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SanDI GG'GwC_CC
Cuts at: 2687 2687
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SfcI C'TryA_G
Cuts at: 826 1045 2604 2614 2991 3113 3775 4910 5591
Size: 219 1559 10 377 122 662 1135 681
Cuts at: 5591 5782 826
Size: 191 1471
Fragments arranged by size:
1559 1471 1135 681 662 377 219 191
122 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2113 3754 1872
Size: 241 1641 4545
Fragments arranged by size:
4545 1641 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3450 4531 5432 5694 820
Size: 197 926 1507 1081 901 262 1553
Fragments arranged by size:
1553 1507 1081 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 3256 4351 5
Size: 47 3204 1095 2081
Fragments arranged by size:
3204 2081 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6427
StyI C'CwwG_G
Cuts at: 513 1065 2004 3866 513
Size: 552 939 1862 3074
Fragments arranged by size:
3074 1862 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 3573 3626 4454 4471 4624 4809
Size: 1255 578 1415 53 828 17 153 185
Cuts at: 4809 5254 325
Size: 445 1498
Fragments arranged by size:
1498 1415 1255 828 578 445 185 153
53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4673 6385 96
Size: 274 80 33 51 1443 2696 1712 138
Fragments arranged by size:
2696 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3578 3578
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 2111 3752 1870
Size: 241 1641 4545
Fragments arranged by size:
4545 1641 241
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PspXI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI