Restriction map for
pFN21AB4266
(Circular) (Six-base) MAPSORT of: pFN21AB4266.seq Check: 6888 from: 1 to: 6238
pFN21AB4266 6238 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 2682 4365 4738 1563
Size: 1119 1683 373 3063
Fragments arranged by size:
3063 1683 1119 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AfeI AGC'GCT
Cuts at: 2645 2645
Size: 6238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 2247 3219 3464 5137 6114 1203
Size: 1044 972 245 1673 977 1327
Fragments arranged by size:
1673 1327 1044 977 972 245
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 2699 1166
Size: 1533 4705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2717 1184
Size: 1533 4705
AlwNI CAG_nnn'CTG
Cuts at: 3981 5553 3981
Size: 1572 4666
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 3486 3547 3773 3925 6034 1493
Size: 1993 61 226 152 2109 1697
Fragments arranged by size:
2109 1993 1697 226 152 61
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2428 3563 1870
Size: 73 485 1135 4545
Fragments arranged by size:
4545 1135 485 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2511 3559 5014 618
Size: 325 217 627 724 1048 1455 1842
Fragments arranged by size:
1842 1455 1048 724 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2313 3259 3557 1391
Size: 733 189 946 298 4072
Fragments arranged by size:
4072 946 733 298 189
BbeI G_GCGC'C
Cuts at: 2515 2515
Size: 6238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 928
Size: 971 286 4981
Fragments arranged by size:
4981 971 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2086 2120 3517 3551 4429 4463 1903
Size: 34 149 34 1397 34 878 34 3678
Fragments arranged by size:
3678 1397 878 149 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 2187 2354
Size: 107 122 71 631 605 442 73 167
Cuts at: 2354 2499 4849 136
Size: 145 2350 1525
Fragments arranged by size:
2350 1525 631 605 442 167 145 122
107 73 71
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 2888 3348 3655 2888
Size: 460 307 5471
Fragments arranged by size:
5471 460 307
Bme1580I G_kGCm'C
Cuts at: 1790 3118 3303 4299 5455 1790
Size: 1328 185 996 1156 2573
Fragments arranged by size:
2573 1328 1156 996 185
BmgBI CAC'GTC
Cuts at: 1318 2248 2305 2920 3234 1318
Size: 930 57 615 314 4322
Fragments arranged by size:
4322 930 615 314 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3969 4927 442
Size: 416 53 83 399 24 2552 958 1753
Fragments arranged by size:
2552 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3027 1056
Size: 1971 4267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 2651 2897 4898 1239
Size: 1232 180 246 2001 2579
Fragments arranged by size:
2579 2001 1232 246 180
Bpu10I CC'TnA_GC
Cuts at: 3337 3337
Size: 6238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 2614 2627 4901 882
Size: 617 792 323 13 2274 2219
Fragments arranged by size:
2274 2219 792 617 323 13
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2190 2346 2409
Size: 53 83 186 936 594 63 156 63
Cuts at: 2409 2512 2754 4045 4427 5062 275
Size: 103 242 1291 382 635 1451
Fragments arranged by size:
1451 1291 936 635 594 382 242 186
156 103 83 63 63 53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6013
Fragments arranged by size:
6013 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2285 2366 2370 1186
Size: 1096 3 81 4 5054
Fragments arranged by size:
5054 1096 81 4 3
BseYI C'CCAG_C
Cuts at: 2239 2923 3131 3400 5441 5937 2239
Size: 684 208 269 2041 496 2540
Fragments arranged by size:
2540 2041 684 496 269 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 1163
Size: 573 480 5185
Fragments arranged by size:
5185 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2179 3537 3688 4449 5477 1540
Size: 275 186 178 1358 151 761 1028 2301
Fragments arranged by size:
2301 1358 1028 761 275 186 178 151
BsiHKAI G_wGCw'C
Cuts at: 3147 3282 4299 4384 5455 3147
Size: 135 1017 85 1071 3930
Fragments arranged by size:
3930 1071 1017 135 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
BsmBI CGTCTCn'nnnn_
Cuts at: 2717 2717
Size: 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2313 3118 3147 3259 3282 3303
Size: 399 334 189 805 29 112 23 21
Cuts at: 3303 3557 4299 4384 5455 1391
Size: 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 805 742 399 334 254 189
112 85 29 23 21
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 3593 844
Size: 2653 96 3489
Fragments arranged by size:
3489 2653 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2260 2892 4125 1394
Size: 429 250 187 632 1233 3507
Fragments arranged by size:
3507 1233 632 429 250 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 4733 4907 65
Size: 1183 1447 2038 174 1396
Fragments arranged by size:
2038 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 4882 5069 1700
Size: 110 683 2389 187 2869
Fragments arranged by size:
2869 2389 683 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 2639 4298 5310 2639
Size: 1659 1012 3567
Fragments arranged by size:
3567 1659 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 3190 1184
Size: 2006 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2467
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 3404 3568 3935 4320 4337 5789 5875 5887
Size: 1432 164 367 385 17 1452 86 12
Cuts at: 5887 6233 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1432 385 367 346 164 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3186 3342 3386 513
Size: 552 939 1182 156 44 3365
Fragments arranged by size:
3365 1182 939 552 156 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3271 487
Size: 683 56 2045 3454
Fragments arranged by size:
3454 2045 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 3754 4548 4568 2233
Size: 1521 794 20 3903
Fragments arranged by size:
3903 1521 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DraIII CAC_nnn'GTG
Cuts at: 2989 2989
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 2856 3389
Size: 54 1425 50 275 18 661 365 533
Cuts at: 3389 3685 4574 6203 8
Size: 296 889 1629 43
Fragments arranged by size:
1629 1425 889 661 533 365 296 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 2600 4168 1353
Size: 1193 54 1568 3423
Fragments arranged by size:
3423 1568 1193 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2132 4825 5199 5345 387
Size: 1064 138 543 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 543 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2651 2897 4301 4898 5685 5953
Size: 393 839 180 246 1404 597 787 268
Cuts at: 5953 1239
Size: 1524
Fragments arranged by size:
1524 1404 839 787 597 393 268 246
180
EcoNI CCTnn'n_nnAGG
Cuts at: 3522 3522
Size: 6238
EcoO109I rG'GnC_Cy
Cuts at: 2548 3001 2548
Size: 453 5785
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
FseI GG_CCGG'CC
Cuts at: 2497 2497
Size: 6238
FspI TGC'GCA
Cuts at: 2084 2954 2963 4744 2084
Size: 870 9 1781 3578
Fragments arranged by size:
3578 1781 870 9
FspAI rTGC'GCAy
Cuts at: 2954 2954
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2379 2397 2467 2474 2515 2540 2647 3138
Size: 701 18 70 7 41 25 107 491
Cuts at: 3138 5385 1678
Size: 2247 2531
Fragments arranged by size:
2531 2247 701 491 107 70 41 25
18 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 4879 4911 4953
Size: 32 135 32 968 32 2307 32 42
Cuts at: 4953 4985 1373
Size: 32 2626
Fragments arranged by size:
2626 2307 968 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KasI G'GCGC_C
Cuts at: 2511 2511
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5352 5536 1305
Size: 4047 184 2007
Fragments arranged by size:
4047 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2858 10
Size: 54 1425 343 1026 3390
Fragments arranged by size:
3390 1425 1026 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 3248 3290 3296 4196 4555 4714
Size: 648 1533 549 42 6 900 359 159
Cuts at: 4714 518
Size: 2042
Fragments arranged by size:
2042 1533 900 648 549 359 159 42
6
MspA1I CmG'CkG
Cuts at: 1606 2517 3388 3400 3472 3654 4331 5479 5724
Size: 911 871 12 72 182 677 1148 245
Cuts at: 5724 1606
Size: 2120
Fragments arranged by size:
2120 1148 911 871 677 245 182 72
12
NaeI GCC'GGC
Cuts at: 2495 2495
Size: 6238
NarI GG'CG_CC
Cuts at: 2512 2512
Size: 6238
NcoI C'CATG_G
Cuts at: 513 1065 2004 3342 513
Size: 552 939 1338 3409
Fragments arranged by size:
3409 1338 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NgoMIV G'CCGG_C
Cuts at: 2493 2493
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 3023 1052
Size: 1971 4267
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3223 3247 3468 3532 3596 5141 1132
Size: 97 1994 24 221 64 64 1545 2229
Fragments arranged by size:
2229 1994 1545 221 97 64 64 24
PasI CC'CwG_GG
Cuts at: 1348 3205 1348
Size: 1857 4381
PciI A'CATG_T
Cuts at: 3219 3464 5137 3219
Size: 245 1673 4320
Fragments arranged by size:
4320 1673 245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2989 1108
Size: 1881 4357
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 3001 2548
Size: 453 5785
PshAI GACnn'nnGTC
Cuts at: 1613 3465 1613
Size: 1852 4386
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 3537 2001
Size: 1536 4702
PvuII CAG'CTG
Cuts at: 3400 3400
Size: 6238
SacII CC_GC'GG
Cuts at: 3389 3389
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SapI GCTCTTCn'nnn_
Cuts at: 2546 2546
Size: 6238
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 3451 4486 6198 3451
Size: 1035 1712 3491
Fragments arranged by size:
3491 1712 1035
SfcI C'TryA_G
Cuts at: 826 1045 3586 4721 5402 5593 826
Size: 219 2541 1135 681 191 1471
Fragments arranged by size:
2541 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2513 2513
Size: 6238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4342 5243 5505 820
Size: 197 926 2399 901 262 1553
Fragments arranged by size:
2399 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3247 3596 1132
Size: 2115 349 3774
Fragments arranged by size:
3774 2115 349
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 2732 1517
Size: 1215 5023
StyI C'CwwG_G
Cuts at: 513 1065 2004 2011 2904 2913 3063 3342 3677
Size: 552 939 7 893 9 150 279 335
Cuts at: 3677 513
Size: 3074
Fragments arranged by size:
3074 939 893 552 335 279 150 9
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 4265 4282 4435 4620 5065 325
Size: 1255 735 1950 17 153 185 445 1498
Fragments arranged by size:
1950 1498 1255 735 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3449 4484 6196
Size: 274 80 33 51 1443 1472 1035 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1712 1472 1443 1035 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 3235 1316
Size: 1919 4319
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2920 3202 2626
Size: 294 282 5662
Fragments arranged by size:
5662 294 282
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 3317 4367 3317
Size: 1050 5188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SrfI SwaI