Restriction map for
pFN21AB4264
(Circular) (Six-base) MAPSORT of: pFN21AB4264.seq Check: 8372 from: 1 to: 6838
pFN21AB4264 6838 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2800 2834 4181 1937
Size: 863 34 1347 4594
Fragments arranged by size:
4594 1347 863 34
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2742 2796 3122 3459 3800 4901 6285
Size: 510 600 54 326 337 341 1101 1384
Cuts at: 6285 6553 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1101 600 510 341 337 326
268 54
AflII C'TTAA_G
Cuts at: 820 1017 2012 2639 820
Size: 197 995 627 5019
Fragments arranged by size:
5019 995 627 197
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2527 3277 1184
Size: 1343 750 4745
Fragments arranged by size:
4745 1343 750
AlwNI CAG_nnn'CTG
Cuts at: 3437 4581 6153 3437
Size: 1144 1572 4122
Fragments arranged by size:
4122 1572 1144
ApaI G_GGCC'C
Cuts at: 3337 3337
Size: 6838
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 3144 3677 3960 4002 4147 4373 4525 6634
Size: 1651 533 283 42 145 226 152 2109
Cuts at: 6634 1493
Size: 1697
Fragments arranged by size:
2109 1697 1651 533 283 226 152 145
42
AseI AT'TA_AT
Cuts at: 160 1032 3531 5392 160
Size: 872 2499 1861 1606
Fragments arranged by size:
2499 1861 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 4163 1870
Size: 73 2220 4545
Fragments arranged by size:
4545 2220 73
BamHI G'GATC_C
Cuts at: 4168 4535 4168
Size: 367 6471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 5614 618
Size: 325 217 627 2372 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3337 4157 1391
Size: 1946 820 4072
Fragments arranged by size:
4072 1946 820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5867
BbvCI CC'TCA_GC
Cuts at: 4091 4091
Size: 6838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5029 5063 1903
Size: 34 3092 34 3678
Fragments arranged by size:
3678 3092 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2275 4730 5940 2275
Size: 2455 1210 3173
Fragments arranged by size:
3173 2455 1210
BclI T'GATC_A
Cuts at: 1548 3826 3840 1548
Size: 2278 14 4546
Fragments arranged by size:
4546 2278 14
BfrBI ATG'CAT
Cuts at: 2875 2875
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3390 5449 136
Size: 107 122 71 631 605 1718 2059 1525
Fragments arranged by size:
2059 1718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3721 6833 3721
Size: 3112 3726
BlpI GC'TnA_GC
Cuts at: 2148 4255 2148
Size: 2107 4731
Bme1580I G_kGCm'C
Cuts at: 1790 2077 3337 4899 6055 1790
Size: 287 1260 1562 1156 2573
Fragments arranged by size:
2573 1562 1260 1156 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6838
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2447 2939 4569
Size: 416 53 83 399 24 1030 492 1630
Cuts at: 4569 5527 442
Size: 958 1753
Fragments arranged by size:
1753 1630 1030 958 492 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 3348 5498 1239
Size: 1008 1101 2150 2579
Fragments arranged by size:
2579 2150 1101 1008
Bpu10I CC'TnA_GC
Cuts at: 4091 4091
Size: 6838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4963 5828 6126 4963
Size: 865 298 5675
Fragments arranged by size:
5675 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2471 5501 882
Size: 617 972 3030 2219
Fragments arranged by size:
3030 2219 972 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4645 5027 5662 275
Size: 53 83 186 936 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3127 5271 5669 5943 6090 6501 3127
Size: 2144 398 274 147 411 3464
Fragments arranged by size:
3464 2144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3294 3324 3735 3765 1178
Size: 30 165 30 1891 30 411 30 4251
Fragments arranged by size:
4251 1891 411 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3336 1186
Size: 2150 4688
BseYI C'CCAG_C
Cuts at: 3298 3425 6041 6537 3298
Size: 127 2616 496 3599
Fragments arranged by size:
3599 2616 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2418 3759 1163
Size: 573 682 1341 4242
Fragments arranged by size:
4242 1341 682 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 3475 4899 4984 6055 2028
Size: 1447 1424 85 1071 2811
Fragments arranged by size:
2811 1447 1424 1071 85
BsmI GAATG_Cn'
Cuts at: 2787 4349 4442 2787
Size: 1562 93 5183
Fragments arranged by size:
5183 1562 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2077 3337 3475 4157 4899 4984
Size: 399 238 49 1260 138 682 742 85
Cuts at: 4984 6055 1391
Size: 1071 2174
Fragments arranged by size:
2174 1260 1071 742 682 399 238 138
85 49
BspEI T'CCGG_A
Cuts at: 3127 6501 3127
Size: 3374 3464
BspHI T'CATG_A
Cuts at: 3363 4727 6457 3363
Size: 1364 1730 3744
Fragments arranged by size:
3744 1730 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 4193 844
Size: 1675 1674 3489
Fragments arranged by size:
3489 1675 1674
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 5333 5507 65
Size: 1183 1212 2873 174 1396
Fragments arranged by size:
2873 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3401 3689 3988 4035 3115
Size: 286 288 299 47 5918
Fragments arranged by size:
5918 299 288 286 47
BstYI r'GATC_y
Cuts at: 1972 2765 3721 4168 4535 4920 4937 6389 6475
Size: 793 956 447 367 385 17 1452 86
Cuts at: 6475 6487 6833 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 956 793 447 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2835 2835
Size: 6838
Bsu36I CC'TnA_GG
Cuts at: 2859 2859
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 2090 3585 4034 513
Size: 552 1025 1495 449 3317
Fragments arranged by size:
3317 1495 1025 552 449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 4354 5148 5168 2430
Size: 1924 794 20 4100
Fragments arranged by size:
4100 1924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 3280 3598 4142 3280
Size: 318 544 5976
Fragments arranged by size:
5976 544 318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2820 2851 3391
Size: 54 1425 50 275 18 990 31 540
Cuts at: 3391 4285 5174 6803 8
Size: 894 889 1629 43
Fragments arranged by size:
1629 1425 990 894 889 540 275 54
50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2751 3600 4768 1353
Size: 1398 849 1168 3423
Fragments arranged by size:
3423 1398 1168 849
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2247 2742 2796 3122 3348 3459
Size: 393 510 105 495 54 326 226 111
Cuts at: 3459 3800 4901 5498 6285 6553 1239
Size: 341 1101 597 787 268 1524
Fragments arranged by size:
1524 1101 787 597 510 495 393 341
326 268 226 111 105 54
EcoO109I rG'GnC_Cy
Cuts at: 3292 3333 3334 3292
Size: 41 1 6796
Fragments arranged by size:
6796 41 1
EcoRI G'AATT_C
Cuts at: 3677 4147 4525 3677
Size: 470 378 5990
Fragments arranged by size:
5990 470 378
EcoRV GAT'ATC
Cuts at: 2659 2997 3919 2659
Size: 338 922 5578
Fragments arranged by size:
5578 922 338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2403 2750 2782 2860
Size: 32 135 32 799 32 347 32 78
Cuts at: 2860 2892 3294 3326 3543 3564 3575 3596 3694
Size: 32 402 32 217 21 11 21 98
Cuts at: 3694 3726 3737 3769 5479 5511 5553 5585 1373
Size: 32 11 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 799 402 347 217 135 98
78 42 32 32 32 32 32 32
32 32 32 32 21 21 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4182 4428 669
Size: 988 281 1935 309 246 3079
Fragments arranged by size:
3079 1935 988 309 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 3387 4437 3387
Size: 1050 5788
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3448 5952 6136 1305
Size: 2143 2504 184 2007
Fragments arranged by size:
2504 2143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 3393 10
Size: 54 1425 343 990 571 3455
Fragments arranged by size:
3455 1425 990 571 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 2828 3113 3584 3687 4796 5155
Size: 648 1518 144 285 471 103 1109 359
Cuts at: 5155 5314 518
Size: 159 2042
Fragments arranged by size:
2042 1518 1109 648 471 359 285 159
144 103
MspA1I CmG'CkG
Cuts at: 1606 4254 4931 6079 6324 1606
Size: 2648 677 1148 245 2120
Fragments arranged by size:
2648 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2090 3585 4034 513
Size: 552 1025 1495 449 3317
Fragments arranged by size:
3317 1495 1025 552 449
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NsiI A_TGCA'T
Cuts at: 2877 2877
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2746 4196 5741 1132
Size: 97 1517 1450 1545 2229
Fragments arranged by size:
2229 1545 1517 1450 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6838
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 2373 3150 4408 2373
Size: 777 1258 4803
Fragments arranged by size:
4803 1258 777
PspOMI G'GGCC_C
Cuts at: 3333 3333
Size: 6838
PstI C_TGCA'G
Cuts at: 830 3020 4190 830
Size: 2190 1170 3478
Fragments arranged by size:
3478 2190 1170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 3016 4186 5321 6002 6193 826
Size: 219 1971 1170 1135 681 191 1471
Fragments arranged by size:
1971 1471 1170 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 2639 4942 5843 6105 820
Size: 197 926 69 627 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 627 262 197 69
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 2837 152
Size: 2685 4153
SphI G_CATG'C
Cuts at: 1132 2746 4196 1132
Size: 1614 1450 3774
Fragments arranged by size:
3774 1614 1450
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6838
StyI C'CwwG_G
Cuts at: 513 1065 2090 2290 3585 4034 4277 513
Size: 552 1025 200 1295 449 243 3074
Fragments arranged by size:
3074 1295 1025 552 449 243 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2950 4865 4882 5035 5220 5665 325
Size: 1255 1370 1915 17 153 185 445 1498
Fragments arranged by size:
1915 1498 1370 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3092 3097
Size: 274 80 33 51 1443 543 572 5
Cuts at: 3097 5084 6796 96
Size: 1987 1712 138
Fragments arranged by size:
1987 1712 1443 572 543 274 138 80
51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6798 6830 6798
Size: 32 6806
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 4967 4967
Size: 6838
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI