Restriction map for
pFN21AB4251
(Circular) (Six-base) MAPSORT of: pFN21AB4251.seq Check: 1570 from: 1 to: 7624
pFN21AB4251 7624 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2474 2143
Size: 331 7293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5434 278
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4420 4967 1937
Size: 2483 547 4594
Fragments arranged by size:
4594 2483 547
Acc65I G'GTAC_C
Cuts at: 4945 4945
Size: 7624
AclI AA'CG_TT
Cuts at: 1563 5751 6124 1563
Size: 4188 373 3063
Fragments arranged by size:
4188 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2509 3520 4145 4388 4490 5687 7071 7339
Size: 877 1011 625 243 102 1197 1384 268
Cuts at: 7339 1632
Size: 1917
Fragments arranged by size:
1917 1384 1197 1011 877 625 268 243
102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7427
AflIII A'CryG_T
Cuts at: 1203 3700 3873 4791 6523 7500 1203
Size: 2497 173 918 1732 977 1327
Fragments arranged by size:
2497 1732 1327 977 918 173
AgeI A'CCGG_T
Cuts at: 6455 6455
Size: 7624
AhdI GACnn_n'nnGTC
Cuts at: 6353 6353
Size: 7624
AleI CACnn'nnGTG
Cuts at: 1166 3591 1166
Size: 2425 5199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3636 1184
Size: 2452 5172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4878 4910 4878
Size: 32 7592
AlwNI CAG_nnn'CTG
Cuts at: 4771 5367 6939 4771
Size: 596 1572 5456
Fragments arranged by size:
5456 1572 596
ApaI G_GGCC'C
Cuts at: 4031 4031
Size: 7624
ApaLI G'TGCA_C
Cuts at: 5681 6837 5681
Size: 1156 6468
ApoI r'AATT_y
Cuts at: 1493 4933 5159 5311 7420 1493
Size: 3440 226 152 2109 1697
Fragments arranged by size:
3440 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6178 160
Size: 872 5146 1606
Fragments arranged by size:
5146 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
AvaI C'yCGr_G
Cuts at: 1870 1943 2538 3858 4323 4949 1870
Size: 73 595 1320 465 626 4545
Fragments arranged by size:
4545 1320 626 595 465 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3262 3295 3262
Size: 33 7591
BamHI G'GATC_C
Cuts at: 2008 2368 4954 5321 2008
Size: 360 2586 367 4311
Fragments arranged by size:
4311 2586 367 360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4811 4945 6400 618
Size: 325 217 627 3024 134 1455 1842
Fragments arranged by size:
3024 1842 1455 627 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2946 3126 3578 3666 3917 4031 4191 4388
Size: 1555 180 452 88 251 114 160 197
Cuts at: 4388 4754 4943 1391
Size: 366 189 4072
Fragments arranged by size:
4072 1555 452 366 251 197 189 180
160 114 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4478 928
Size: 971 2579 4074
Fragments arranged by size:
4074 2579 971
BbvCI CC'TCA_GC
Cuts at: 3990 3990
Size: 7624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2758 2792 5815 5849 1903
Size: 34 821 34 3023 34 3678
Fragments arranged by size:
3678 3023 821 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5516 6726 5516
Size: 1210 6414
BclI T'GATC_A
Cuts at: 1548 4756 1548
Size: 3208 4416
BfrBI ATG'CAT
Cuts at: 3187 3814 3187
Size: 627 6997
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3900 4129 4383
Size: 107 122 71 631 605 2228 229 254
Cuts at: 4383 6235 136
Size: 1852 1525
Fragments arranged by size:
2228 1852 1525 631 605 254 229 122
107 71
BglII A'GATC_T
Cuts at: 3078 7619 3078
Size: 4541 3083
BlpI GC'TnA_GC
Cuts at: 3558 5041 3558
Size: 1483 6141
Bme1580I G_kGCm'C
Cuts at: 1790 3826 4031 4089 5685 6841 1790
Size: 2036 205 58 1596 1156 2573
Fragments arranged by size:
2573 2036 1596 1156 205 58
BmgBI CAC'GTC
Cuts at: 1318 3701 1318
Size: 2383 5241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 2480 2572
Size: 416 53 83 399 24 633 430 92
Cuts at: 2572 3426 3775 4076 4248 5355 6313 442
Size: 854 349 301 172 1107 958 1753
Fragments arranged by size:
1753 1107 958 854 633 430 416 399
349 301 172 92 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3102 3137 4856 1056
Size: 2046 35 1719 3824
Fragments arranged by size:
3824 2046 1719 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2965 2368
Size: 597 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2580 2960 6284 1239
Size: 1341 380 3324 2579
Fragments arranged by size:
3324 2579 1341 380
Bpu10I CC'TnA_GC
Cuts at: 3489 3990 4748 3489
Size: 501 758 6365
Fragments arranged by size:
6365 758 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2891 5749 6614 6912 2891
Size: 2858 865 298 3603
Fragments arranged by size:
3603 2858 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2224 2402 3780 6287 882
Size: 617 725 178 1378 2507 2219
Fragments arranged by size:
2507 2219 1378 725 617 178
BsaAI yAC'GTr
Cuts at: 493 1204 4034 5415 493
Size: 711 2830 1381 2702
Fragments arranged by size:
2830 2702 1381 711
BsaBI GATnn'nnATC
Cuts at: 4671 7618 4671
Size: 2947 4677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5431 5813 6448 275
Size: 53 83 186 936 3898 382 635 1451
Fragments arranged by size:
3898 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3506 6057 6455 6729 6876 7287 3506
Size: 2551 398 274 147 411 3843
Fragments arranged by size:
3843 2551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4603 4633 1178
Size: 30 165 30 3200 30 4169
Fragments arranged by size:
4169 3200 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4587 1186
Size: 3401 4223
BseYI C'CCAG_C
Cuts at: 2113 2387 6827 7323 2113
Size: 274 4440 496 2414
Fragments arranged by size:
4440 2414 496 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 4094 1163
Size: 573 837 1521 4693
Fragments arranged by size:
4693 1521 837 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5074 5835 6863 1540
Size: 275 186 3073 761 1028 2301
Fragments arranged by size:
3073 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2134 4191 5685 5770 6841 2134
Size: 2057 1494 85 1071 2917
Fragments arranged by size:
2917 2057 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 2961 3608 4707 5135 5228 2961
Size: 647 1099 428 93 5357
Fragments arranged by size:
5357 1099 647 428 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2946 3126 3578 3666 3826 3917
Size: 399 344 812 180 452 88 160 91
Cuts at: 3917 4031 4089 4191 4388 4754 4943 5685 5770
Size: 114 58 102 197 366 189 742 85
Cuts at: 5770 6841 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 812 742 452 399 366 344
197 189 180 160 114 102 91 88
85 58
BspEI T'CCGG_A
Cuts at: 7287 7287
Size: 7624
BspHI T'CATG_A
Cuts at: 4882 5513 7243 4882
Size: 631 1730 5263
Fragments arranged by size:
5263 1730 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 2474 4979 844
Size: 1299 331 2505 3489
Fragments arranged by size:
3489 2505 1299 331
BsrBI CCG'CTC
Cuts at: 1394 1823 5511 1394
Size: 429 3688 3507
Fragments arranged by size:
3688 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3413 3852 6119 6293 65
Size: 1183 2165 439 2267 174 1396
Fragments arranged by size:
2267 2165 1396 1183 439 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2807 3890 3919 6268 6455 1700
Size: 110 997 1083 29 2349 187 2869
Fragments arranged by size:
2869 2349 1083 997 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 3682 96
Size: 3586 4038
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7624
BssSI C'ACGA_G
Cuts at: 4379 5684 6696 4379
Size: 1305 1012 5307
Fragments arranged by size:
5307 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7624
BstBI TT'CG_AA
Cuts at: 4108 6428 4108
Size: 2320 5304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7624
BstXI CCAn_nnnn'nTGG
Cuts at: 2085 2156 3924 4546 2085
Size: 71 1768 622 5163
Fragments arranged by size:
5163 1768 622 71
BstYI r'GATC_y
Cuts at: 1972 2008 2368 2831 2934 3078 4954 5321 5706
Size: 36 360 463 103 144 1876 367 385
Cuts at: 5706 5723 7175 7261 7273 7619 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1876 1452 463 385 367 360 346
144 103 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4272 513
Size: 552 939 2268 3865
Fragments arranged by size:
3865 2268 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6885
Fragments arranged by size:
6885 683 56
BtsI GCAGTG_nn'
Cuts at: 2817 5140 5934 5954 2817
Size: 2323 794 20 4487
Fragments arranged by size:
4487 2323 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7589
DraI TTT'AAA
Cuts at: 4928 4928
Size: 7624
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6631 809
Size: 507 5315 1802
Fragments arranged by size:
5315 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 3083 4462
Size: 54 1425 50 275 18 257 996 1379
Cuts at: 4462 5071 5960 7589 8
Size: 609 889 1629 43
Fragments arranged by size:
1629 1425 1379 996 889 609 275 257
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5071 1537
Size: 275 3259 4090
Fragments arranged by size:
4090 3259 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2434 2470 3131 3396 5554 1353
Size: 1081 36 661 265 2158 3423
Fragments arranged by size:
3423 2158 1081 661 265 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6211 6585 6731 387
Size: 1064 138 4622 374 146 1280
Fragments arranged by size:
4622 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4189 4189
Size: 7624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2509 2580 2960 3520 4145 4388 4490
Size: 393 877 71 380 560 625 243 102
Cuts at: 4490 5687 6284 7071 7339 1239
Size: 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 877 787 625 597 560 393
380 268 243 102 71
EcoO109I rG'GnC_Cy
Cuts at: 2543 2543
Size: 7624
EcoRI G'AATT_C
Cuts at: 4933 5311 4933
Size: 378 7246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3343 3550 3343
Size: 207 7417
FspI TGC'GCA
Cuts at: 6130 6130
Size: 7624
HaeII r_GCGC'y
Cuts at: 1678 6771 1678
Size: 5093 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2676 2708 2823 2855 3063
Size: 32 135 32 1104 32 115 32 208
Cuts at: 3063 3095 3852 3884 4330 4362 4439 4471 4741
Size: 32 757 32 446 32 77 32 270
Cuts at: 4741 4773 6265 6297 6339 6371 1373
Size: 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 1104 757 446 270 208 135
115 77 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3871 4968 5214 669
Size: 988 281 1933 1097 246 3079
Fragments arranged by size:
3079 1933 1097 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2094 2855 3014 3236 4518 748
Size: 1346 761 159 222 1282 3854
Fragments arranged by size:
3854 1346 1282 761 222 159
HpaI GTT'AAC
Cuts at: 5214 5214
Size: 7624
KpnI G_GTAC'C
Cuts at: 4949 4949
Size: 7624
MfeI C'AATT_G
Cuts at: 5223 5223
Size: 7624
MluI A'CGCG_T
Cuts at: 7500 7500
Size: 7624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2041 2402 3858 6738 6922 1305
Size: 736 361 1456 2880 184 2007
Fragments arranged by size:
2880 2007 1456 736 361 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2089 3085 4464 10
Size: 54 1425 343 257 996 1379 3170
Fragments arranged by size:
3170 1425 1379 996 343 257 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2056 3591 3902 3908 4022 5582 5941
Size: 648 890 1535 311 6 114 1560 359
Cuts at: 5941 6100 518
Size: 159 2042
Fragments arranged by size:
2042 1560 1535 890 648 359 311 159
114 6
MspA1I CmG'CkG
Cuts at: 1606 2554 4900 5040 5717 6865 7110 1606
Size: 948 2346 140 677 1148 245 2120
Fragments arranged by size:
2346 2120 1148 948 677 245 140
NaeI GCC'GGC
Cuts at: 3892 3892
Size: 7624
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6133
Fragments arranged by size:
6133 939 552
NdeI CA'TA_TG
Cuts at: 387 4761 387
Size: 4374 3250
NgoMIV G'CCGG_C
Cuts at: 3890 3890
Size: 7624
NheI G'CTAG_C
Cuts at: 1052 3098 3133 4852 1052
Size: 2046 35 1719 3824
Fragments arranged by size:
3824 2046 1719 35
NotI GC'GGCC_GC
Cuts at: 5071 5071
Size: 7624
NsiI A_TGCA'T
Cuts at: 3189 3816 3189
Size: 627 6997
NspI r_CATG'y
Cuts at: 1132 1229 2785 3191 3877 3907 4267 4795 4982
Size: 97 1556 406 686 30 360 528 187
Cuts at: 4982 6527 1132
Size: 1545 2229
Fragments arranged by size:
2229 1556 1545 686 528 406 360 187
97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7624
PciI A'CATG_T
Cuts at: 3873 4791 6523 3873
Size: 918 1732 4974
Fragments arranged by size:
4974 1732 918
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2364 1108
Size: 1256 6368
PfoI T'CCnGG_A
Cuts at: 2937 3219 4621 2937
Size: 282 1402 5940
Fragments arranged by size:
5940 1402 282
PmeI GTTT'AAAC
Cuts at: 4928 4928
Size: 7624
PmlI CAC'GTG
Cuts at: 4034 4034
Size: 7624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 5693 5725 7206 7238 4898
Size: 32 763 32 1481 32 5284
Fragments arranged by size:
5284 1481 763 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2543 2543
Size: 7624
PshAI GACnn'nnGTC
Cuts at: 1613 3959 1613
Size: 2346 5278
PsiI TTA'TAA
Cuts at: 5194 5194
Size: 7624
PspOMI G'GGCC_C
Cuts at: 4027 4027
Size: 7624
PstI C_TGCA'G
Cuts at: 830 2140 4976 830
Size: 1310 2836 3478
Fragments arranged by size:
3478 2836 1310
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7624
PvuII CAG'CTG
Cuts at: 2554 4900 2554
Size: 2346 5278
SacI G_AGCT'C
Cuts at: 4191 4191
Size: 7624
SalI G'TCGA_C
Cuts at: 1936 4966 1936
Size: 3030 4594
SanDI GG'GwC_CC
Cuts at: 2543 2543
Size: 7624
SapI GCTCTTCn'nnn_
Cuts at: 2470 3131 2470
Size: 661 6963
SbfI CC_TGCA'GG
Cuts at: 2140 4976 2140
Size: 2836 4788
ScaI AGT'ACT
Cuts at: 4576 5872 7584 4576
Size: 1296 1712 4616
Fragments arranged by size:
4616 1712 1296
SfcI C'TryA_G
Cuts at: 826 1045 2136 3059 3370 4502 4972 6107 6788
Size: 219 1091 923 311 1132 470 1135 681
Cuts at: 6788 6979 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1132 1091 923 681 470 311
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7624
SmaI CCC'GGG
Cuts at: 1872 2540 4325 4951 1872
Size: 668 1785 626 4545
Fragments arranged by size:
4545 1785 668 626
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3858 5728 6629 6891 820
Size: 197 926 927 988 1870 901 262 1553
Fragments arranged by size:
1870 1553 988 927 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5415 493
Size: 4922 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7624
SphI G_CATG'C
Cuts at: 1132 3191 3907 4267 4982 1132
Size: 2059 716 360 715 3774
Fragments arranged by size:
3774 2059 716 715 360
SspI AAT'ATT
Cuts at: 5 52 3253 5548 5
Size: 47 3201 2295 2081
Fragments arranged by size:
3201 2295 2081 47
StuI AGG'CCT
Cuts at: 1517 2235 1517
Size: 718 6906
StyI C'CwwG_G
Cuts at: 513 1065 2004 5063 513
Size: 552 939 3059 3074
Fragments arranged by size:
3074 3059 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 3960 4169 4651 5651 5668 5821
Size: 1255 484 1896 209 482 1000 17 153
Cuts at: 5821 6006 6451 325
Size: 185 445 1498
Fragments arranged by size:
1896 1498 1255 1000 484 482 445 209
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3230 3682 4574
Size: 274 80 33 51 1443 1253 452 892
Cuts at: 4574 4794 5870 7582 96
Size: 220 1076 1712 138
Fragments arranged by size:
1712 1443 1253 1076 892 452 274 220
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3154 3186 7584 7616 3154
Size: 32 4398 32 3162
Fragments arranged by size:
4398 3162 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7624
XbaI T'CTAG_A
Cuts at: 4960 4960
Size: 7624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3550 3550
Size: 7624
XhoI C'TCGA_G
Cuts at: 1943 3858 1943
Size: 1915 5709
XmaI C'CCGG_G
Cuts at: 1870 2538 4323 4949 1870
Size: 668 1785 626 4545
Fragments arranged by size:
4545 1785 668 626
XmnI GAAnn'nnTTC
Cuts at: 2280 3533 4913 5753 2280
Size: 1253 1380 840 4151
Fragments arranged by size:
4151 1380 1253 840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5432 276
Size: 53 83 186 936 3898 2468
Fragments arranged by size:
3898 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI