Restriction map for
pFN21AB4239
(Circular) (Six-base) MAPSORT of: pFN21AB4239.seq Check: 8439 from: 1 to: 8329
pFN21AB4239 8329 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4528 6139 278
Size: 53 83 186 936 2992 1611 2468
Fragments arranged by size:
2992 2468 1611 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 5672 1937
Size: 607 3128 4594
Fragments arranged by size:
4594 3128 607
Acc65I G'GTAC_C
Cuts at: 5650 5650
Size: 8329
AclI AA'CG_TT
Cuts at: 1563 6456 6829 1563
Size: 4893 373 3063
Fragments arranged by size:
4893 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 2817 3087 5512 6392 7776 8044 1632
Size: 444 741 270 2425 880 1384 268 1917
Fragments arranged by size:
2425 1917 1384 880 741 444 270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8132
AflIII A'CryG_T
Cuts at: 1203 7228 8205 1203
Size: 6025 977 1327
Fragments arranged by size:
6025 1327 977
AgeI A'CCGG_T
Cuts at: 7160 7160
Size: 8329
AhdI GACnn_n'nnGTC
Cuts at: 4396 7058 4396
Size: 2662 5667
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8329
AlwNI CAG_nnn'CTG
Cuts at: 6072 7644 6072
Size: 1572 6757
ApaLI G'TGCA_C
Cuts at: 6386 7542 6386
Size: 1156 7173
ApoI r'AATT_y
Cuts at: 1493 2063 2375 2399 2686 3947 4664 4973 5376
Size: 570 312 24 287 1261 717 309 403
Cuts at: 5376 5638 5864 6016 8125 1493
Size: 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1261 717 570 403 312 309
287 262 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 3687 6883 160
Size: 872 2655 3196 1606
Fragments arranged by size:
3196 2655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8329
AvaI C'yCGr_G
Cuts at: 1870 1943 5654 1870
Size: 73 3711 4545
Fragments arranged by size:
4545 3711 73
BamHI G'GATC_C
Cuts at: 2283 3841 5659 6026 2283
Size: 1558 1818 367 4586
Fragments arranged by size:
4586 1818 1558 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4938 5552 5650 7105 618
Size: 325 217 627 3151 614 98 1455 1842
Fragments arranged by size:
3151 1842 1455 627 614 325 217 98
BanII G_rGCy'C
Cuts at: 1391 4273 5648 1391
Size: 2882 1375 4072
Fragments arranged by size:
4072 2882 1375
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 3139 4428 928
Size: 971 840 400 1289 4829
Fragments arranged by size:
4829 1289 971 840 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3329 3363 3449 3483 6520 6554 1903
Size: 34 1392 34 86 34 3037 34 3678
Fragments arranged by size:
3678 3037 1392 86 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 6221 7431 3590
Size: 2631 1210 4488
Fragments arranged by size:
4488 2631 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 6940 136
Size: 107 122 71 631 605 3264 2004 1525
Fragments arranged by size:
3264 2004 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2563 8324 2563
Size: 5761 2568
BlpI GC'TnA_GC
Cuts at: 5746 5746
Size: 8329
Bme1580I G_kGCm'C
Cuts at: 1790 2301 6390 7546 1790
Size: 511 4089 1156 2573
Fragments arranged by size:
4089 2573 1156 511
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 3107 4268
Size: 416 53 83 399 24 940 750 1161
Cuts at: 4268 5096 6060 7018 442
Size: 828 964 958 1753
Fragments arranged by size:
1753 1161 964 958 940 828 750 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 5047 2920
Size: 2127 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3422 3679 3927 4119 4425 4437 6989 1239
Size: 2183 257 248 192 306 12 2552 2579
Fragments arranged by size:
2579 2552 2183 306 257 248 192 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 3801 5002 5226 6454 7319 7617 2804
Size: 997 1201 224 1228 865 298 3516
Fragments arranged by size:
3516 1228 1201 997 865 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5508 5528 6992 882
Size: 617 4009 20 1464 2219
Fragments arranged by size:
4009 2219 1464 617 20
BsaAI yAC'GTr
Cuts at: 493 1204 6120 493
Size: 711 4916 2702
Fragments arranged by size:
4916 2702 711
BsaBI GATnn'nnATC
Cuts at: 4143 5361 8323 4143
Size: 1218 2962 4149
Fragments arranged by size:
4149 2962 1218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4325 4525 6136 6518
Size: 53 83 186 936 2792 200 1611 382
Cuts at: 6518 7153 275
Size: 635 1451
Fragments arranged by size:
2792 1611 1451 936 635 382 200 186
83 53
BsaWI w'CCGG_w
Cuts at: 3035 3838 6762 7160 7434 7581 7992 3035
Size: 803 2924 398 274 147 411 3372
Fragments arranged by size:
3372 2924 803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4260 4290 5041 5071 1178
Size: 30 165 30 2857 30 751 30 4436
Fragments arranged by size:
4436 2857 751 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 2985 1186
Size: 1264 535 6530
Fragments arranged by size:
6530 1264 535
BseYI C'CCAG_C
Cuts at: 3470 4931 7532 8028 3470
Size: 1461 2601 496 3771
Fragments arranged by size:
3771 2601 1461 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2917 1163
Size: 573 1181 6575
Fragments arranged by size:
6575 1181 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5779 6540 7568 1540
Size: 275 186 3778 761 1028 2301
Fragments arranged by size:
3778 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6390 6475 7546 6390
Size: 85 1071 7173
Fragments arranged by size:
7173 1071 85
BsmI GAATG_Cn'
Cuts at: 4960 5840 5933 4960
Size: 880 93 7356
Fragments arranged by size:
7356 880 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 4273 5648 6390 6475 7546 1391
Size: 399 511 1972 1375 742 85 1071 2174
Fragments arranged by size:
2174 1972 1375 1071 742 511 399 85
BspEI T'CCGG_A
Cuts at: 3838 7992 3838
Size: 4154 4175
BspHI T'CATG_A
Cuts at: 4114 6218 7948 4114
Size: 2104 1730 4495
Fragments arranged by size:
4495 2104 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5684 844
Size: 4840 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6216 1394
Size: 429 4393 3507
Fragments arranged by size:
4393 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 3049 3334 3437 4310 5599 6824
Size: 1183 1360 441 285 103 873 1289 1225
Cuts at: 6824 6998 65
Size: 174 1396
Fragments arranged by size:
1396 1360 1289 1225 1183 873 441 285
174 103
BsrFI r'CCGG_y
Cuts at: 1700 1810 6973 7160 1700
Size: 110 5163 187 2869
Fragments arranged by size:
5163 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2647 96
Size: 2551 5778
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8329
BssSI C'ACGA_G
Cuts at: 6389 7401 6389
Size: 1012 7317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8329
BstBI TT'CG_AA
Cuts at: 7133 7133
Size: 8329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8329
BstXI CCAn_nnnn'nTGG
Cuts at: 4281 4281
Size: 8329
BstYI r'GATC_y
Cuts at: 1972 2283 2443 2563 3841 4379 5564 5579 5659
Size: 311 160 120 1278 538 1185 15 80
Cuts at: 5659 6026 6411 6428 7880 7966 7978 8324 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1278 1185 538 385 367 346
311 160 120 86 80 17 15 12
BstZ17I GTA'TAC
Cuts at: 2545 2545
Size: 8329
Bsu36I CC'TnA_GG
Cuts at: 3357 5038 3357
Size: 1681 6648
BtgI C'CryG_G
Cuts at: 513 1065 2004 3223 4215 4450 5518 513
Size: 552 939 1219 992 235 1068 3324
Fragments arranged by size:
3324 1219 1068 992 939 552 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7590
Fragments arranged by size:
7590 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 4794 5845 6639 6659 2746
Size: 2048 1051 794 20 4416
Fragments arranged by size:
4416 2048 1051 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2818 2853 529
Size: 35 2254 35 6005
Fragments arranged by size:
6005 2254 35 35
DraI TTT'AAA
Cuts at: 5633 5633
Size: 8329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5495 7336 809
Size: 507 4179 1841 1802
Fragments arranged by size:
4179 1841 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5776 6665 8294
Size: 54 1425 50 275 18 3946 889 1629
Cuts at: 8294 8
Size: 43
Fragments arranged by size:
3946 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5776 1537
Size: 275 3964 4090
Fragments arranged by size:
4090 3964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 2982 3960 5283 5309 5531 6259 1353
Size: 667 962 978 1323 26 222 728 3423
Fragments arranged by size:
3423 1323 978 962 728 667 222 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6916 7290 7436 387
Size: 1064 138 5327 374 146 1280
Fragments arranged by size:
5327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2817 3087 3422 3679 3927 4119
Size: 393 444 741 270 335 257 248 192
Cuts at: 4119 4425 4437 5512 6392 6989 7776 8044 1239
Size: 306 12 1075 880 597 787 268 1524
Fragments arranged by size:
1524 1075 880 787 741 597 444 393
335 306 270 268 257 248 192 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2472 5436 2472
Size: 2964 5365
EcoRI G'AATT_C
Cuts at: 5638 6016 5638
Size: 378 7951
EcoRV GAT'ATC
Cuts at: 5070 5070
Size: 8329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4737 5462 4737
Size: 725 7604
FspI TGC'GCA
Cuts at: 6835 6835
Size: 8329
HaeII r_GCGC'y
Cuts at: 1678 7476 1678
Size: 5798 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 3458 3490 4382
Size: 32 135 32 447 32 1407 32 892
Cuts at: 4382 4414 5219 5251 5564 5596 6970 7002 7044
Size: 32 805 32 313 32 1374 32 42
Cuts at: 7044 7076 1373
Size: 32 2626
Fragments arranged by size:
2626 1407 1374 892 805 447 313 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3118 4497 5673 5919 669
Size: 988 281 739 441 1379 1176 246 3079
Fragments arranged by size:
3079 1379 1176 988 739 441 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8329
HpaI GTT'AAC
Cuts at: 2677 5919 2677
Size: 3242 5087
KpnI G_GTAC'C
Cuts at: 5654 5654
Size: 8329
MfeI C'AATT_G
Cuts at: 2076 2448 5928 2076
Size: 372 3480 4477
Fragments arranged by size:
4477 3480 372
MluI A'CGCG_T
Cuts at: 8205 8205
Size: 8329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2132 4551 7443 7627 1305
Size: 827 2419 2892 184 2007
Fragments arranged by size:
2892 2419 2007 827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6507
Fragments arranged by size:
6507 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2206 3423 4067 6287 6646 6805 518
Size: 648 1040 1217 644 2220 359 159 2042
Fragments arranged by size:
2220 2042 1217 1040 648 644 359 159
MspA1I CmG'CkG
Cuts at: 1606 2328 4935 5745 6422 7570 7815 1606
Size: 722 2607 810 677 1148 245 2120
Fragments arranged by size:
2607 2120 1148 810 722 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3223 4215 4450 5518 513
Size: 552 939 1219 992 235 1068 3324
Fragments arranged by size:
3324 1219 1068 992 939 552 235
NdeI CA'TA_TG
Cuts at: 387 3594 387
Size: 3207 5122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8329
NotI GC'GGCC_GC
Cuts at: 5776 5776
Size: 8329
NspI r_CATG'y
Cuts at: 1132 1229 5687 7232 1132
Size: 97 4458 1545 2229
Fragments arranged by size:
4458 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3153 1348
Size: 1805 6524
PciI A'CATG_T
Cuts at: 7228 7228
Size: 8329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3134 4456 1108
Size: 2026 1322 4981
Fragments arranged by size:
4981 2026 1322
PfoI T'CCnGG_A
Cuts at: 3656 3656
Size: 8329
PmeI GTTT'AAAC
Cuts at: 5633 5633
Size: 8329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2979 6398 6430 7911 7943 2947
Size: 32 3419 32 1481 32 3333
Fragments arranged by size:
3419 3333 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3995 5520 1613
Size: 2382 1525 4422
Fragments arranged by size:
4422 2382 1525
PsiI TTA'TAA
Cuts at: 4193 4466 5899 4193
Size: 273 1433 6623
Fragments arranged by size:
6623 1433 273
PstI C_TGCA'G
Cuts at: 830 3517 5681 830
Size: 2687 2164 3478
Fragments arranged by size:
3478 2687 2164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8329
PvuII CAG'CTG
Cuts at: 4935 4935
Size: 8329
SalI G'TCGA_C
Cuts at: 1936 5671 1936
Size: 3735 4594
SapI GCTCTTCn'nnn_
Cuts at: 2982 3960 5283 2982
Size: 978 1323 6028
Fragments arranged by size:
6028 1323 978
SbfI CC_TGCA'GG
Cuts at: 5681 5681
Size: 8329
ScaI AGT'ACT
Cuts at: 5336 6577 8289 5336
Size: 1241 1712 5376
Fragments arranged by size:
5376 1712 1241
SexAI A'CCwGG_T
Cuts at: 4490 4490
Size: 8329
SfcI C'TryA_G
Cuts at: 826 1045 3306 3501 3513 5604 5677 6812 7493
Size: 219 2261 195 12 2091 73 1135 681
Cuts at: 7493 7684 826
Size: 191 1471
Fragments arranged by size:
2261 2091 1471 1135 681 219 195 191
73 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8329
SmaI CCC'GGG
Cuts at: 1872 5656 1872
Size: 3784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 3816 4981 5241 6433 7334
Size: 197 926 840 1033 1165 260 1192 901
Cuts at: 7334 7596 820
Size: 262 1553
Fragments arranged by size:
1553 1192 1165 1033 926 901 840 262
260 197
SnaBI TAC'GTA
Cuts at: 493 6120 493
Size: 5627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8329
SphI G_CATG'C
Cuts at: 1132 5687 1132
Size: 4555 3774
SspI AAT'ATT
Cuts at: 5 52 2509 3250 3928 6253 5
Size: 47 2457 741 678 2325 2081
Fragments arranged by size:
2457 2325 2081 741 678 47
StuI AGG'CCT
Cuts at: 1517 2478 4236 1517
Size: 961 1758 5610
Fragments arranged by size:
5610 1758 961
StyI C'CwwG_G
Cuts at: 513 1065 2004 3223 3760 4000 4179 4215 4450
Size: 552 939 1219 537 240 179 36 235
Cuts at: 4450 5518 5768 513
Size: 1068 250 3074
Fragments arranged by size:
3074 1219 1068 939 552 537 250 240
235 179 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5115 5439 6356 6373 6526 6711 7156
Size: 1255 3535 324 917 17 153 185 445
Cuts at: 7156 325
Size: 1498
Fragments arranged by size:
3535 1498 1255 917 445 324 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2647 4242 4472
Size: 274 80 33 51 1443 670 1595 230
Cuts at: 4472 4516 4968 5334 6575 8287 96
Size: 44 452 366 1241 1712 138
Fragments arranged by size:
1712 1595 1443 1241 670 452 366 274
230 138 80 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4260 4292 5311 5343 8289 8321 4260
Size: 32 1019 32 2946 32 4268
Fragments arranged by size:
4268 2946 1019 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8329
XbaI T'CTAG_A
Cuts at: 3793 5665 3793
Size: 1872 6457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2205 2820 2947 2205
Size: 615 127 7587
Fragments arranged by size:
7587 615 127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8329
XmaI C'CCGG_G
Cuts at: 1870 5654 1870
Size: 3784 4545
XmnI GAAnn'nnTTC
Cuts at: 3855 6458 3855
Size: 2603 5726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4526 6137 276
Size: 53 83 186 936 2992 1611 2468
Fragments arranged by size:
2992 2468 1611 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI