Restriction map for
pFN21AB4231
(Circular) (Six-base) MAPSORT of: pFN21AB4231.seq Check: 7756 from: 1 to: 10252
pFN21AB4231 10252 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8062 278
Size: 53 83 186 936 6526 2468
Fragments arranged by size:
6526 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4894 5500 5637 7595 1937
Size: 2957 606 137 1958 4594
Fragments arranged by size:
4594 2957 1958 606 137
Acc65I G'GTAC_C
Cuts at: 7573 7573
Size: 10252
AclI AA'CG_TT
Cuts at: 1563 8379 8752 1563
Size: 6816 373 3063
Fragments arranged by size:
6816 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 2913 3207 3581 4215 4623 4935 5168
Size: 1184 97 294 374 634 408 312 233
Cuts at: 5168 5677 6144 7184 8315 9699 9967 1632
Size: 509 467 1040 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1184 1131 1040 634 509 467
408 374 312 294 268 233 97
AflII C'TTAA_G
Cuts at: 820 1017 5713 820
Size: 197 4696 5359
Fragments arranged by size:
5359 4696 197
AflIII A'CryG_T
Cuts at: 1203 2031 2534 9151 10128 1203
Size: 828 503 6617 977 1327
Fragments arranged by size:
6617 1327 977 828 503
AgeI A'CCGG_T
Cuts at: 9083 9083
Size: 10252
AhdI GACnn_n'nnGTC
Cuts at: 8981 8981
Size: 10252
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4662 4694 5880 5912 4662
Size: 32 1186 32 9002
Fragments arranged by size:
9002 1186 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2849 3658 7995 9567 2849
Size: 809 4337 1572 3534
Fragments arranged by size:
4337 3534 1572 809
ApaI G_GGCC'C
Cuts at: 2426 2426
Size: 10252
ApaLI G'TGCA_C
Cuts at: 8309 9465 8309
Size: 1156 9096
ApoI r'AATT_y
Cuts at: 1493 2221 2499 3126 3217 3544 3591 4000 4274
Size: 728 278 627 91 327 47 409 274
Cuts at: 4274 4412 5128 5273 5562 5990 6545 6824 7561
Size: 138 716 145 289 428 555 279 737
Cuts at: 7561 7787 7939 10048 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 737 728 716 627 555 428
409 327 289 279 278 274 226 152
145 138 91 47
AseI AT'TA_AT
Cuts at: 160 1032 2381 3438 4403 8806 160
Size: 872 1349 1057 965 4403 1606
Fragments arranged by size:
4403 1606 1349 1057 965 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 7577 1870
Size: 73 100 5534 4545
Fragments arranged by size:
5534 4545 100 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3979 4012 4902 4935 3979
Size: 33 890 33 9296
Fragments arranged by size:
9296 890 33 33
BamHI G'GATC_C
Cuts at: 7582 7949 7582
Size: 367 9885
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4184 4804 7573 9028 618
Size: 325 217 627 2397 620 2769 1455 1842
Fragments arranged by size:
2769 2397 1842 1455 627 620 325 217
BanII G_rGCy'C
Cuts at: 1391 2050 2426 7571 1391
Size: 659 376 5145 4072
Fragments arranged by size:
5145 4072 659 376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 2822 4364 4732 6132 6901 928
Size: 971 128 795 1542 368 1400 769 4279
Fragments arranged by size:
4279 1542 1400 971 795 769 368 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8443 8477 1903
Size: 34 6506 34 3678
Fragments arranged by size:
6506 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4462 6685 8144 9354 4462
Size: 2223 1459 1210 5360
Fragments arranged by size:
5360 2223 1459 1210
BclI T'GATC_A
Cuts at: 1548 2767 2899 3631 5866 1548
Size: 1219 132 732 2235 5934
Fragments arranged by size:
5934 2235 1219 732 132
BfrBI ATG'CAT
Cuts at: 4298 4968 4997 7403 4298
Size: 670 29 2406 7147
Fragments arranged by size:
7147 2406 670 29
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8863 136
Size: 107 122 71 631 605 7191 1525
Fragments arranged by size:
7191 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10247 10247
Size: 10252
BlpI GC'TnA_GC
Cuts at: 6453 7669 6453
Size: 1216 9036
Bme1580I G_kGCm'C
Cuts at: 1790 2337 2426 5913 8313 9469 1790
Size: 547 89 3487 2400 1156 2573
Fragments arranged by size:
3487 2573 2400 1156 547 89
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7983 8941 442
Size: 416 53 83 399 24 6566 958 1753
Fragments arranged by size:
6566 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2181 2181
Size: 10252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2337 2691 4028 5006 5211 6663 8912
Size: 833 265 354 1337 978 205 1452 2249
Cuts at: 8912 1239
Size: 2579
Fragments arranged by size:
2579 2249 1452 1337 978 833 354 265
205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3759 4227 5665 5787 6984 8377 9242 9540 3759
Size: 468 1438 122 1197 1393 865 298 4471
Fragments arranged by size:
4471 1438 1393 1197 865 468 298 122
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5562 5754 7166 8915 882
Size: 617 4063 192 1412 1749 2219
Fragments arranged by size:
4063 2219 1749 1412 617 192
BsaAI yAC'GTr
Cuts at: 493 1204 8043 493
Size: 711 6839 2702
Fragments arranged by size:
6839 2702 711
BsaBI GATnn'nnATC
Cuts at: 10246 10246
Size: 10252
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4865 8059 8441 9076
Size: 53 83 186 936 3332 3194 382 635
Cuts at: 9076 275
Size: 1451
Fragments arranged by size:
3332 3194 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5042 7209 8685 9083 9357 9504 9915 5042
Size: 2167 1476 398 274 147 411 5379
Fragments arranged by size:
5379 2167 1476 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4146 4176 5289 5319 1178
Size: 30 165 30 2743 30 1113 30 6111
Fragments arranged by size:
6111 2743 1113 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2154 7262 1186
Size: 968 5108 4176
Fragments arranged by size:
5108 4176 968
BseYI C'CCAG_C
Cuts at: 2097 4637 6916 9455 9951 2097
Size: 2540 2279 2539 496 2398
Fragments arranged by size:
2540 2539 2398 2279 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2895 3084 3693 6491 6565 6893 1163
Size: 573 1159 189 609 2798 74 328 4522
Fragments arranged by size:
4522 2798 1159 609 573 328 189 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 7702 8463 9491 1540
Size: 275 186 2398 3303 761 1028 2301
Fragments arranged by size:
3303 2398 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 8313 8398 9469 8313
Size: 85 1071 9096
Fragments arranged by size:
9096 1071 85
BsmI GAATG_Cn'
Cuts at: 3406 3851 4439 4880 4977 5894 7200 7763 7856
Size: 445 588 441 97 917 1306 563 93
Cuts at: 7856 3406
Size: 5802
Fragments arranged by size:
5802 1306 917 588 563 445 441 97
93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2337 2426 5913 7571 8313 8398
Size: 399 260 287 89 3487 1658 742 85
Cuts at: 8398 9469 1391
Size: 1071 2174
Fragments arranged by size:
3487 2174 1658 1071 742 399 287 260
89 85
BspEI T'CCGG_A
Cuts at: 7209 9915 7209
Size: 2706 7546
BspHI T'CATG_A
Cuts at: 2503 3346 6721 8141 9871 2503
Size: 843 3375 1420 1730 2884
Fragments arranged by size:
3375 2884 1730 1420 843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7607 844
Size: 6763 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 8139 1394
Size: 429 6316 3507
Fragments arranged by size:
6316 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 3371 6007 8747 8921 65
Size: 1183 1105 1018 2636 2740 174 1396
Fragments arranged by size:
2740 2636 1396 1183 1105 1018 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8896 9083 1700
Size: 110 7086 187 2869
Fragments arranged by size:
7086 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2989 7215 96
Size: 2893 4226 3133
Fragments arranged by size:
4226 3133 2893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10252
BssSI C'ACGA_G
Cuts at: 2720 8312 9324 2720
Size: 5592 1012 3648
Fragments arranged by size:
5592 3648 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2112 6383 794
Size: 1318 4271 4663
Fragments arranged by size:
4663 4271 1318
BstBI TT'CG_AA
Cuts at: 9056 9056
Size: 10252
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10252
BstXI CCAn_nnnn'nTGG
Cuts at: 7183 7183
Size: 10252
BstYI r'GATC_y
Cuts at: 1972 2858 3334 5237 5396 7582 7949 8334 8351
Size: 886 476 1903 159 2186 367 385 17
Cuts at: 8351 9803 9889 9901 10247 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2186 1977 1903 1452 886 476 385 367
346 159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3827 6078 3827
Size: 2251 8001
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 9700
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6117 487
Size: 683 56 4891 4622
Fragments arranged by size:
4891 4622 683 56
BtsI GCAGTG_nn'
Cuts at: 5979 7285 7768 8562 8582 5979
Size: 1306 483 794 20 7649
Fragments arranged by size:
7649 1306 794 483 20
ClaI AT'CG_AT
Cuts at: 1554 4395 4399 1554
Size: 2841 4 7407
Fragments arranged by size:
7407 2841 4
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6726 6761 529
Size: 35 6162 35 4020
Fragments arranged by size:
6162 4020 35 35
DraI TTT'AAA
Cuts at: 3586 3999 7393 7556 3586
Size: 413 3394 163 6282
Fragments arranged by size:
6282 3394 413 163
DraIII CAC_nnn'GTG
Cuts at: 3239 3239
Size: 10252
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6905 9259 809
Size: 507 5589 2354 1802
Fragments arranged by size:
5589 2354 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 4758 6250
Size: 54 1425 50 275 18 234 2694 1492
Cuts at: 6250 7699 8588 10217 8
Size: 1449 889 1629 43
Fragments arranged by size:
2694 1629 1492 1449 1425 889 275 234
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7699 1537
Size: 275 5887 4090
Fragments arranged by size:
5887 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 4599 7211 8182 1353
Size: 1843 1403 2612 971 3423
Fragments arranged by size:
3423 2612 1843 1403 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8839 9213 9359 387
Size: 1064 138 7250 374 146 1280
Fragments arranged by size:
7250 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2337 2691 2816 2913 3207 3581
Size: 393 440 265 354 125 97 294 374
Cuts at: 3581 4028 4215 4623 4935 5006 5168 5211 5677
Size: 447 187 408 312 71 162 43 466
Cuts at: 5677 6144 6663 7184 8315 8912 9699 9967 1239
Size: 467 519 521 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 787 597 521 519 467 466
447 440 408 393 374 354 312 294
268 265 187 162 125 97 71 43
EcoO109I rG'GnC_Cy
Cuts at: 2422 2423 2794 3227 3407 4084 2422
Size: 1 371 433 180 677 8590
Fragments arranged by size:
8590 677 433 371 180 1
EcoRI G'AATT_C
Cuts at: 3126 7561 7939 3126
Size: 4435 378 5439
Fragments arranged by size:
5439 4435 378
EcoRV GAT'ATC
Cuts at: 4650 6592 7512 4650
Size: 1942 920 7390
Fragments arranged by size:
7390 1942 920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2813 3289 4196 5258 5699 5728 2263
Size: 550 476 907 1062 441 29 6787
Fragments arranged by size:
6787 1062 907 550 476 441 29
FspI TGC'GCA
Cuts at: 2105 7199 8758 2105
Size: 5094 1559 3599
Fragments arranged by size:
5094 3599 1559
HaeII r_GCGC'y
Cuts at: 1678 9399 1678
Size: 7721 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2714 2746 2752
Size: 32 135 32 460 32 650 32 6
Cuts at: 2752 2759 2784 2791 3167 3171 3199 3203 3326
Size: 7 25 7 376 4 28 4 123
Cuts at: 3326 3358 3874 3906 4001 4033 4437 4469 4598
Size: 32 516 32 95 32 404 32 129
Cuts at: 4598 4630 4938 4970 6683 6715 8893 8925 8967
Size: 32 308 32 1713 32 2178 32 42
Cuts at: 8967 8999 1373
Size: 32 2626
Fragments arranged by size:
2626 2178 1713 650 516 460 404 376
308 135 129 123 95 42 32 32
32 32 32 32 32 32 32 32
32 32 32 28 25 7 7 6
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4506 6624 7596 7842 669
Size: 988 281 2076 492 2118 972 246 3079
Fragments arranged by size:
3079 2118 2076 988 972 492 281 246
HindIII A'AGCT_T
Cuts at: 748 2109 748
Size: 1361 8891
HpaI GTT'AAC
Cuts at: 7842 7842
Size: 10252
KpnI G_GTAC'C
Cuts at: 7577 7577
Size: 10252
MfeI C'AATT_G
Cuts at: 7851 7851
Size: 10252
MluI A'CGCG_T
Cuts at: 10128 10128
Size: 10252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3007 5145 5275 5777 7122 9366 9550 1305
Size: 1702 2138 130 502 1345 2244 184 2007
Fragments arranged by size:
2244 2138 2007 1702 1345 502 184 130
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4760 6252 10
Size: 54 1425 343 2928 1492 4010
Fragments arranged by size:
4010 2928 1492 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 2811 2964 4565 5109 6023 6258
Size: 648 1437 208 153 1601 544 914 235
Cuts at: 6258 8210 8569 8728 518
Size: 1952 359 159 2042
Fragments arranged by size:
2042 1952 1601 1437 914 648 544 359
235 208 159 153
MspA1I CmG'CkG
Cuts at: 1606 2101 2449 2915 4984 6877 7668 8345 9493
Size: 495 348 466 2069 1893 791 677 1148
Cuts at: 9493 9738 1606
Size: 245 2120
Fragments arranged by size:
2120 2069 1893 1148 791 677 495 466
348 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9700
NdeI CA'TA_TG
Cuts at: 387 4300 4999 6247 387
Size: 3913 699 1248 4392
Fragments arranged by size:
4392 3913 1248 699
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10252
NotI GC'GGCC_GC
Cuts at: 7699 7699
Size: 10252
NsiI A_TGCA'T
Cuts at: 4300 4970 4999 7405 4300
Size: 670 29 2406 7147
Fragments arranged by size:
7147 2406 670 29
NspI r_CATG'y
Cuts at: 1132 1229 2035 2268 2538 4838 4968 6617 7610
Size: 97 806 233 270 2300 130 1649 993
Cuts at: 7610 9155 1132
Size: 1545 2229
Fragments arranged by size:
2300 2229 1649 1545 993 806 270 233
130 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10252
PciI A'CATG_T
Cuts at: 2031 2534 9151 2031
Size: 503 6617 3132
Fragments arranged by size:
6617 3132 503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10252
PfoI T'CCnGG_A
Cuts at: 3130 5964 3130
Size: 2834 7418
PmeI GTTT'AAAC
Cuts at: 7556 7556
Size: 10252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 8321 8353 9834 9866 5825
Size: 32 2464 32 1481 32 6211
Fragments arranged by size:
6211 2464 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 3227 3407 4084 2794
Size: 433 180 677 8962
Fragments arranged by size:
8962 677 433 180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10252
PsiI TTA'TAA
Cuts at: 7822 7822
Size: 10252
PspOMI G'GGCC_C
Cuts at: 2422 2422
Size: 10252
PstI C_TGCA'G
Cuts at: 830 2454 2479 2914 3399 6958 7280 7604 830
Size: 1624 25 435 485 3559 322 324 3478
Fragments arranged by size:
3559 3478 1624 485 435 324 322 25
PvuI CG_AT'CG
Cuts at: 2001 4399 2001
Size: 2398 7854
PvuII CAG'CTG
Cuts at: 2101 2449 2915 4984 6877 2101
Size: 348 466 2069 1893 5476
Fragments arranged by size:
5476 2069 1893 466 348
SalI G'TCGA_C
Cuts at: 1936 7594 1936
Size: 5658 4594
SbfI CC_TGCA'GG
Cuts at: 7604 7604
Size: 10252
ScaI AGT'ACT
Cuts at: 2243 6420 8500 10212 2243
Size: 4177 2080 1712 2283
Fragments arranged by size:
4177 2283 2080 1712
SexAI A'CCwGG_T
Cuts at: 3235 3235
Size: 10252
SfcI C'TryA_G
Cuts at: 826 1045 2450 2475 2910 3084 3395 4039 5376
Size: 219 1405 25 435 174 311 644 1337
Cuts at: 5376 6711 6816 6954 7276 7600 8735 9416 9607
Size: 1335 105 138 322 324 1135 681 191
Cuts at: 9607 826
Size: 1471
Fragments arranged by size:
1471 1405 1337 1335 1135 681 644 435
324 322 311 219 191 174 138 105
25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10252
SmaI CCC'GGG
Cuts at: 1872 2045 7579 1872
Size: 173 5534 4545
Fragments arranged by size:
5534 4545 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3774 4242 5644 5713 5802 6999
Size: 197 926 1831 468 1402 69 89 1197
Cuts at: 6999 8356 9257 9519 820
Size: 1357 901 262 1553
Fragments arranged by size:
1831 1553 1402 1357 1197 926 901 468
262 197 89 69
SnaBI TAC'GTA
Cuts at: 493 8043 493
Size: 7550 2702
SpeI A'CTAG_T
Cuts at: 152 3748 152
Size: 3596 6656
SphI G_CATG'C
Cuts at: 1132 2268 4968 7610 1132
Size: 1136 2700 2642 3774
Fragments arranged by size:
3774 2700 2642 1136
SspI AAT'ATT
Cuts at: 5 52 3112 5388 8176 5
Size: 47 3060 2276 2788 2081
Fragments arranged by size:
3060 2788 2276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10252
StyI C'CwwG_G
Cuts at: 513 1065 4441 6737 7006 7691 513
Size: 552 3376 2296 269 685 3074
Fragments arranged by size:
3376 3074 2296 685 552 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 5499 5589 6127 8279 8296 8449
Size: 1255 2658 1261 90 538 2152 17 153
Cuts at: 8449 8634 9079 325
Size: 185 445 1498
Fragments arranged by size:
2658 2152 1498 1261 1255 538 445 185
153 90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 2294 2360
Size: 274 80 33 51 1443 264 53 66
Cuts at: 2360 2515 2989 4043 6418 6430 7215 8498 10210
Size: 155 474 1054 2375 12 785 1283 1712
Cuts at: 10210 96
Size: 138
Fragments arranged by size:
2375 1712 1443 1283 1054 785 474 274
264 155 138 80 66 53 51 33
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4051 4083 5178 5210 5289 5321 10212 10244 4051
Size: 32 1095 32 79 32 4891 32 4059
Fragments arranged by size:
4891 4059 1095 79 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10252
XbaI T'CTAG_A
Cuts at: 2302 4357 7588 2302
Size: 2055 3231 4966
Fragments arranged by size:
4966 3231 2055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3920 4852 3920
Size: 932 9320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10252
XmaI C'CCGG_G
Cuts at: 1870 2043 7577 1870
Size: 173 5534 4545
Fragments arranged by size:
5534 4545 173
XmnI GAAnn'nnTTC
Cuts at: 2033 2442 3111 3220 4365 5708 5837 6033 8381
Size: 409 669 109 1145 1343 129 196 2348
Cuts at: 8381 2033
Size: 3904
Fragments arranged by size:
3904 2348 1343 1145 669 409 196 129
109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8060 276
Size: 53 83 186 936 6526 2468
Fragments arranged by size:
6526 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI