Restriction map for
pFN21AB4223
(Circular) (Six-base) MAPSORT of: pFN21AB4223.seq Check: 1550 from: 1 to: 5953
pFN21AB4223 5953 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3048 3763 278
Size: 53 83 186 936 1512 715 2468
Fragments arranged by size:
2468 1512 936 715 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 2846 4016 5400 5668 1632
Size: 1184 30 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1184 1170 268 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 2414 2613 4852 5829 1203
Size: 1211 199 2239 977 1327
Fragments arranged by size:
2239 1327 1211 977 199
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 5086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 5921
AlwNI CAG_nnn'CTG
Cuts at: 2554 2752 3696 5268 2554
Size: 198 944 1572 3239
Fragments arranged by size:
3239 1572 944 198
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2359 3026 3262 3488 3640 5749 1493
Size: 866 667 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 667 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3278 1870
Size: 73 1335 4545
Fragments arranged by size:
4545 1335 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 3274 4729 618
Size: 325 217 627 257 1230 1455 1842
Fragments arranged by size:
1842 1455 1230 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 3272 1391
Size: 1881 4072
BbeI G_GCGC'C
Cuts at: 2048 2048
Size: 5953
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4982
BbvCI CC'TCA_GC
Cuts at: 2132 2996 2132
Size: 864 5089
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 2344 1548
Size: 796 5157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3186 5948 3186
Size: 2762 3191
BlpI GC'TnA_GC
Cuts at: 2051 2174 3370 2051
Size: 123 1196 4634
Fragments arranged by size:
4634 1196 123
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 2473 2866 1318
Size: 1155 393 4405
Fragments arranged by size:
4405 1155 393
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2807 4613 1239
Size: 1568 1806 2579
Fragments arranged by size:
2579 1806 1568
Bpu10I CC'TnA_GC
Cuts at: 2132 2996 3204 2132
Size: 864 208 4881
Fragments arranged by size:
4881 864 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 4078 4943 5241 2639
Size: 1439 865 298 3351
Fragments arranged by size:
3351 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3045 3760 4142
Size: 53 83 186 936 512 1000 715 382
Cuts at: 4142 4777 275
Size: 635 1451
Fragments arranged by size:
1451 1000 936 715 635 512 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2974 4386 4784 5058 5205 5616 2974
Size: 1412 398 274 147 411 3311
Fragments arranged by size:
3311 1412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2900 2915 2933 2984 1186
Size: 1594 120 15 18 51 4155
Fragments arranged by size:
4155 1594 120 51 18 15
BseYI C'CCAG_C
Cuts at: 2273 5156 5652 2273
Size: 2883 496 2574
Fragments arranged by size:
2883 2574 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2002 3129 1163
Size: 573 266 1127 3987
Fragments arranged by size:
3987 1127 573 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2437 3403 4164 5192 1540
Size: 275 186 436 966 761 1028 2301
Fragments arranged by size:
2301 1028 966 761 436 275 186
BsiHKAI G_wGCw'C
Cuts at: 4014 4099 5170 4014
Size: 85 1071 4797
Fragments arranged by size:
4797 1071 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3272 4014 4099 5170 1391
Size: 399 1482 742 85 1071 2174
Fragments arranged by size:
2174 1482 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2974 5616 2974
Size: 2642 3311
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 3308 844
Size: 1433 1031 3489
Fragments arranged by size:
3489 1433 1031
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2905 3840 1394
Size: 429 227 855 935 3507
Fragments arranged by size:
3507 935 855 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 4448 4622 65
Size: 1183 937 2263 174 1396
Fragments arranged by size:
2263 1396 1183 937 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2436 4597 4784 1700
Size: 110 626 2161 187 2869
Fragments arranged by size:
2869 2161 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2752 3152 794
Size: 1958 400 3595
Fragments arranged by size:
3595 1958 400
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 2602 1184
Size: 1418 4535
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 3186 3283 3650 4035 4052 5504 5590 5602
Size: 1214 97 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1214 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2304 2574 513
Size: 552 963 276 270 3892
Fragments arranged by size:
3892 963 552 276 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2624 487
Size: 683 56 1398 3816
Fragments arranged by size:
3816 1398 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 4263 4283 3469
Size: 794 20 5139
Fragments arranged by size:
5139 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DraIII CAC_nnn'GTG
Cuts at: 2866 2866
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3099 3400 4289
Size: 54 1425 50 275 18 1269 301 889
Cuts at: 4289 5918 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1269 889 301 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 3883 1353
Size: 905 1625 3423
Fragments arranged by size:
3423 1625 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 4540 4914 5060 387
Size: 1064 138 903 2048 374 146 1280
Fragments arranged by size:
2048 1280 1064 903 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2807 2816 2846 4016 4613 5400 5668
Size: 393 1175 9 30 1170 597 787 268
Cuts at: 5668 1239
Size: 1524
Fragments arranged by size:
1524 1175 1170 787 597 393 268 30
9
EcoRI G'AATT_C
Cuts at: 2359 3026 3262 3640 2359
Size: 667 236 378 4672
Fragments arranged by size:
4672 667 378 236
EcoRV GAT'ATC
Cuts at: 3112 3112
Size: 5953
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2048 2058 2379 2443 5100 1678
Size: 370 10 321 64 2657 2531
Fragments arranged by size:
2657 2531 370 321 64 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3221 3253 4594 4626 4668
Size: 32 135 32 1649 32 1341 32 42
Cuts at: 4668 4700 1373
Size: 32 2626
Fragments arranged by size:
2626 1649 1341 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3043 3297 3543 669
Size: 988 281 1105 254 246 3079
Fragments arranged by size:
3079 1105 988 281 254 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KasI G'GCGC_C
Cuts at: 2044 2044
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 2414 5829 2414
Size: 3415 2538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 3264 5067 5251 1305
Size: 732 1227 1803 184 2007
Fragments arranged by size:
2007 1803 1227 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3101 10
Size: 54 1425 343 1269 2862
Fragments arranged by size:
2862 1425 1269 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2201 3911 4270 4429 518
Size: 648 867 168 1710 359 159 2042
Fragments arranged by size:
2042 1710 867 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2824 3369 4046 5194 5439 1606
Size: 1218 545 677 1148 245 2120
Fragments arranged by size:
2120 1218 1148 677 545 245
NarI GG'CG_CC
Cuts at: 2045 2045
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 4162
Fragments arranged by size:
4162 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2563 2759 3152 3311 4856 1132
Size: 97 1334 196 393 159 1545 2229
Fragments arranged by size:
2229 1545 1334 393 196 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 4195
PfoI T'CCnGG_A
Cuts at: 2784 2784
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PstI C_TGCA'G
Cuts at: 830 3305 830
Size: 2475 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2824 2824
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 3212 4201 5913 3212
Size: 989 1712 3252
Fragments arranged by size:
3252 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 2224 2839 3301 4436 5117 5308 826
Size: 219 1179 615 462 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 615 462 219 191
SfoI GGC'GCC
Cuts at: 2046 2046
Size: 5953
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 4057 4958 5220 820
Size: 197 926 711 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2304 2700 3392 513
Size: 552 1239 396 692 3074
Fragments arranged by size:
3074 1239 692 552 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3980 3997 4150 4335 4780 325
Size: 1255 1350 1050 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 1050 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3210 4199 5911
Size: 274 80 33 51 1443 1233 989 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
1712 1443 1233 989 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2906 2938 5913 5945 2906
Size: 32 2975 32 2914
Fragments arranged by size:
2975 2914 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2458 1316
Size: 1142 4811
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2470 2405
Size: 65 5888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 2108 2615 4082 2084
Size: 24 507 1467 3955
Fragments arranged by size:
3955 1467 507 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3046 3761 276
Size: 53 83 186 936 1512 715 2468
Fragments arranged by size:
2468 1512 936 715 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI