Restriction map for
pFN21AB4222
(Circular) (Six-base) MAPSORT of: pFN21AB4222.seq Check: 5177 from: 1 to: 7726
pFN21AB4222 7726 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3809 5536 278
Size: 53 83 186 936 2273 1727 2468
Fragments arranged by size:
2468 2273 1727 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 3748 3756 3809 5069 1937
Size: 517 1294 8 53 1260 4594
Fragments arranged by size:
4594 1294 1260 517 53 8
Acc65I G'GTAC_C
Cuts at: 5047 5047
Size: 7726
AclI AA'CG_TT
Cuts at: 1563 5853 6226 1563
Size: 4290 373 3063
Fragments arranged by size:
4290 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 4361 4424 5789 7173 7441 1632
Size: 947 1782 63 1365 1384 268 1917
Fragments arranged by size:
1917 1782 1384 1365 947 268 63
AfeI AGC'GCT
Cuts at: 2366 2366
Size: 7726
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7529
AflIII A'CryG_T
Cuts at: 1203 3334 3617 4642 6625 7602 1203
Size: 2131 283 1025 1983 977 1327
Fragments arranged by size:
2131 1983 1327 1025 977 283
AgeI A'CCGG_T
Cuts at: 6557 6557
Size: 7726
AhdI GACnn_n'nnGTC
Cuts at: 6455 6455
Size: 7726
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7726
AlwNI CAG_nnn'CTG
Cuts at: 5469 7041 5469
Size: 1572 6154
ApaLI G'TGCA_C
Cuts at: 4657 5783 6939 4657
Size: 1126 1156 5444
Fragments arranged by size:
5444 1156 1126
ApoI r'AATT_y
Cuts at: 1493 2504 3161 3465 5035 5261 5413 7522 1493
Size: 1011 657 304 1570 226 152 2109 1697
Fragments arranged by size:
2109 1697 1570 1011 657 304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6280 160
Size: 872 5248 1606
Fragments arranged by size:
5248 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7726
AvaI C'yCGr_G
Cuts at: 1870 1943 4587 5051 1870
Size: 73 2644 464 4545
Fragments arranged by size:
4545 2644 464 73
BamHI G'GATC_C
Cuts at: 2312 2944 4365 5056 5423 2312
Size: 632 1421 691 367 4615
Fragments arranged by size:
4615 1421 691 632 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4475 4909 5015 5047 6502
Size: 325 217 627 2688 434 106 32 1455
Cuts at: 6502 618
Size: 1842
Fragments arranged by size:
2688 1842 1455 627 434 325 217 106
32
BanII G_rGCy'C
Cuts at: 1391 4989 5045 1391
Size: 3598 56 4072
Fragments arranged by size:
4072 3598 56
BbeI G_GCGC'C
Cuts at: 4479 4479
Size: 7726
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5917 5951 1903
Size: 34 3980 34 3678
Fragments arranged by size:
3980 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5618 6828 4523
Size: 1095 1210 5421
Fragments arranged by size:
5421 1210 1095
BclI T'GATC_A
Cuts at: 1548 4137 1548
Size: 2589 5137
BfrBI ATG'CAT
Cuts at: 2341 2341
Size: 7726
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6337 136
Size: 107 122 71 631 605 4665 1525
Fragments arranged by size:
4665 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2172 7721 2172
Size: 5549 2177
BlpI GC'TnA_GC
Cuts at: 5143 5143
Size: 7726
Bme1580I G_kGCm'C
Cuts at: 1790 4661 4912 5787 6943 1790
Size: 2871 251 875 1156 2573
Fragments arranged by size:
2871 2573 1156 875 251
BmgBI CAC'GTC
Cuts at: 1318 2304 4563 4643 1318
Size: 986 2259 80 4401
Fragments arranged by size:
4401 2259 986 80
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5457 6415 442
Size: 416 53 83 399 24 4040 958 1753
Fragments arranged by size:
4040 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 3050 3171 3570 3992 4193 4391 4577
Size: 1064 747 121 399 422 201 198 186
Cuts at: 4577 4646 4772 6386 1239
Size: 69 126 1614 2579
Fragments arranged by size:
2579 1614 1064 747 422 399 201 198
186 126 121 69
Bpu10I CC'TnA_GC
Cuts at: 4878 4878
Size: 7726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5851 6716 7014 5851
Size: 865 298 6563
Fragments arranged by size:
6563 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2052 3110 3376 6389 882
Size: 617 553 1058 266 3013 2219
Fragments arranged by size:
3013 2219 1058 617 553 266
BsaAI yAC'GTr
Cuts at: 493 1204 3335 3472 5517 493
Size: 711 2131 137 2045 2702
Fragments arranged by size:
2702 2131 2045 711 137
BsaBI GATnn'nnATC
Cuts at: 3935 7720 3935
Size: 3785 3941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 3806 4476 5533
Size: 53 83 186 936 801 1472 670 1057
Cuts at: 5533 5915 6550 275
Size: 382 635 1451
Fragments arranged by size:
1472 1451 1057 936 801 670 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3478 6159 6557 6831 6978 7389 3478
Size: 2681 398 274 147 411 3815
Fragments arranged by size:
3815 2681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2380 2410 3525 3555 3568
Size: 30 165 30 977 30 1115 30 13
Cuts at: 3568 3598 4550 4580 1178
Size: 30 952 30 4324
Fragments arranged by size:
4324 1115 977 952 165 30 30 30
30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 1186
Size: 997 6729
BseYI C'CCAG_C
Cuts at: 2375 6929 7425 2375
Size: 4554 496 2676
Fragments arranged by size:
4554 2676 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2284 1163
Size: 573 548 6605
Fragments arranged by size:
6605 573 548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5176 5937 6965 1540
Size: 275 186 3175 761 1028 2301
Fragments arranged by size:
3175 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4632 4661 4884 5787 5872 6943 4632
Size: 29 223 903 85 1071 5415
Fragments arranged by size:
5415 1071 903 223 85 29
BsmI GAATG_Cn'
Cuts at: 2074 4188 5237 5330 2074
Size: 2114 1049 93 4470
Fragments arranged by size:
4470 2114 1049 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4553 4553
Size: 7726
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4632 4661 4884 4912 4989 5045 5787
Size: 399 2842 29 223 28 77 56 742
Cuts at: 5787 5872 6943 1391
Size: 85 1071 2174
Fragments arranged by size:
2842 2174 1071 742 399 223 85 77
56 29 28
BspEI T'CCGG_A
Cuts at: 7389 7389
Size: 7726
BspHI T'CATG_A
Cuts at: 5615 7345 5615
Size: 1730 5996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5081 844
Size: 4237 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5613 1394
Size: 429 3790 3507
Fragments arranged by size:
3790 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3720 4156 6221 6395 65
Size: 1183 2472 436 2065 174 1396
Fragments arranged by size:
2472 2065 1396 1183 436 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6370 6557 1700
Size: 110 4560 187 2869
Fragments arranged by size:
4560 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3853 96
Size: 3757 3969
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7726
BssSI C'ACGA_G
Cuts at: 5786 6798 5786
Size: 1012 6714
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7726
BstBI TT'CG_AA
Cuts at: 3244 3652 6530 3244
Size: 408 2878 4440
Fragments arranged by size:
4440 2878 408
BstEII G'GTnAC_C
Cuts at: 1184 2150 1184
Size: 966 6760
BstYI r'GATC_y
Cuts at: 1972 2172 2312 2944 3845 4365 4812 5056 5423
Size: 200 140 632 901 520 447 244 367
Cuts at: 5423 5808 5825 7277 7363 7375 7721 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 901 632 520 447 385 367
346 244 200 140 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2539 2902 513
Size: 552 939 535 363 5337
Fragments arranged by size:
5337 939 552 535 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6987
Fragments arranged by size:
6987 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 5242 6036 6056 2139
Size: 3103 794 20 3809
Fragments arranged by size:
3809 3103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7726
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2620 2655 3112 3147 529
Size: 35 2056 35 457 35 5108
Fragments arranged by size:
5108 2056 457 35 35 35
DraI TTT'AAA
Cuts at: 5030 5030
Size: 7726
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6733 809
Size: 507 5417 1802
Fragments arranged by size:
5417 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4429 5173 6062
Size: 54 1425 50 275 18 2599 744 889
Cuts at: 6062 7691 8
Size: 1629 43
Fragments arranged by size:
2599 1629 1425 889 744 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5173 1537
Size: 275 3361 4090
Fragments arranged by size:
4090 3361 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 3219 5656 1353
Size: 1007 859 2437 3423
Fragments arranged by size:
3423 2437 1007 859
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 6313 6687 6833 387
Size: 1064 138 2558 2166 374 146 1280
Fragments arranged by size:
2558 2166 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2579 3050 3171 3570 3992 4193
Size: 393 671 276 471 121 399 422 201
Cuts at: 4193 4361 4391 4424 4577 4646 4772 5789 6386
Size: 168 30 33 153 69 126 1017 597
Cuts at: 6386 7173 7441 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1017 787 671 597 471 422 399
393 276 268 201 168 153 126 121
69 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3270 3270
Size: 7726
EcoO109I rG'GnC_Cy
Cuts at: 2379 4012 2379
Size: 1633 6093
EcoRI G'AATT_C
Cuts at: 5035 5413 5035
Size: 378 7348
EcoRV GAT'ATC
Cuts at: 2536 2536
Size: 7726
FspI TGC'GCA
Cuts at: 6232 6232
Size: 7726
HaeII r_GCGC'y
Cuts at: 1678 2368 3106 4479 6873 1678
Size: 690 738 1373 2394 2531
Fragments arranged by size:
2531 2394 1373 738 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2617 2649 3422
Size: 32 135 32 980 32 33 32 773
Cuts at: 3422 3454 3525 3557 4430 4462 4804 4836 6367
Size: 32 71 32 873 32 342 32 1531
Cuts at: 6367 6399 6441 6473 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 980 873 773 342 135 71
42 33 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3147 5070 5316 669
Size: 988 281 909 300 1923 246 3079
Fragments arranged by size:
3079 1923 988 909 300 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7726
HpaI GTT'AAC
Cuts at: 2847 5316 2847
Size: 2469 5257
KasI G'GCGC_C
Cuts at: 4475 4475
Size: 7726
KpnI G_GTAC'C
Cuts at: 5051 5051
Size: 7726
MfeI C'AATT_G
Cuts at: 2835 4701 5325 2835
Size: 1866 624 5236
Fragments arranged by size:
5236 1866 624
MluI A'CGCG_T
Cuts at: 7602 7602
Size: 7726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2997 3093 6840 7024 1305
Size: 1692 96 3747 184 2007
Fragments arranged by size:
3747 2007 1692 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4431 10
Size: 54 1425 343 2599 3305
Fragments arranged by size:
3305 2599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4550 4641 5684 6043 6202 518
Size: 648 3384 91 1043 359 159 2042
Fragments arranged by size:
3384 2042 1043 648 359 159 91
MspA1I CmG'CkG
Cuts at: 1606 2375 2893 4235 5142 5819 6967 7212 1606
Size: 769 518 1342 907 677 1148 245 2120
Fragments arranged by size:
2120 1342 1148 907 769 677 518 245
NarI GG'CG_CC
Cuts at: 4476 4476
Size: 7726
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6235
Fragments arranged by size:
6235 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7726
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7726
NotI GC'GGCC_GC
Cuts at: 5173 5173
Size: 7726
NsiI A_TGCA'T
Cuts at: 2343 2343
Size: 7726
NspI r_CATG'y
Cuts at: 1132 1229 3319 4076 4117 5084 6629 1132
Size: 97 2090 757 41 967 1545 2229
Fragments arranged by size:
2229 2090 1545 967 757 97 41
PasI CC'CwG_GG
Cuts at: 1348 4981 1348
Size: 3633 4093
PciI A'CATG_T
Cuts at: 6625 6625
Size: 7726
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 2390 1108
Size: 1218 64 6444
Fragments arranged by size:
6444 1218 64
PfoI T'CCnGG_A
Cuts at: 4008 4368 4008
Size: 360 7366
PmeI GTTT'AAAC
Cuts at: 5030 5030
Size: 7726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5795 5827 7308 7340 5795
Size: 32 1481 32 6181
Fragments arranged by size:
6181 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2379 4012 2379
Size: 1633 6093
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7726
PsiI TTA'TAA
Cuts at: 2786 5296 2786
Size: 2510 5216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3330 3362 3330
Size: 32 7694
PstI C_TGCA'G
Cuts at: 830 5078 830
Size: 4248 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7726
PvuII CAG'CTG
Cuts at: 2375 4235 2375
Size: 1860 5866
SalI G'TCGA_C
Cuts at: 1936 5068 1936
Size: 3132 4594
SanDI GG'GwC_CC
Cuts at: 2379 2379
Size: 7726
SapI GCTCTTCn'nnn_
Cuts at: 3219 3219
Size: 7726
SbfI CC_TGCA'GG
Cuts at: 5078 5078
Size: 7726
ScaI AGT'ACT
Cuts at: 5974 7686 5974
Size: 1712 6014
SfcI C'TryA_G
Cuts at: 826 1045 2715 5074 6209 6890 7081 826
Size: 219 1670 2359 1135 681 191 1471
Fragments arranged by size:
2359 1670 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 4477 4477
Size: 7726
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7726
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7726
SmaI CCC'GGG
Cuts at: 1872 4589 5053 1872
Size: 2717 464 4545
Fragments arranged by size:
4545 2717 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5830 6731 6993 820
Size: 197 926 3887 901 262 1553
Fragments arranged by size:
3887 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3472 5517 493
Size: 2979 2045 2702
Fragments arranged by size:
2979 2702 2045
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7726
SphI G_CATG'C
Cuts at: 1132 5084 1132
Size: 3952 3774
SspI AAT'ATT
Cuts at: 5 52 5650 5
Size: 47 5598 2081
Fragments arranged by size:
5598 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7726
StyI C'CwwG_G
Cuts at: 513 1065 2004 2384 3205 5165 513
Size: 552 939 380 821 1960 3074
Fragments arranged by size:
3074 1960 939 821 552 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3051 3243 5025 5753 5770 5923 6108
Size: 1255 1471 192 1782 728 17 153 185
Cuts at: 6108 6553 325
Size: 445 1498
Fragments arranged by size:
1782 1498 1471 1255 728 445 192 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3853 4443 5972
Size: 274 80 33 51 1443 1876 590 1529
Cuts at: 5972 7684 96
Size: 1712 138
Fragments arranged by size:
1876 1712 1529 1443 590 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 3026 3058 4350 4382 4550 4582 7686
Size: 32 614 32 1292 32 168 32 3104
Cuts at: 7686 7718 2380
Size: 32 2388
Fragments arranged by size:
3104 2388 1292 614 168 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2564 1316
Size: 1248 6478
XbaI T'CTAG_A
Cuts at: 5062 5062
Size: 7726
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3424
Size: 7726
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7726
XmaI C'CCGG_G
Cuts at: 1870 4587 5051 1870
Size: 2717 464 4545
Fragments arranged by size:
4545 2717 464
XmnI GAAnn'nnTTC
Cuts at: 2920 5855 2920
Size: 2935 4791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3807 5534 276
Size: 53 83 186 936 2273 1727 2468
Fragments arranged by size:
2468 2273 1727 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI
RsrII SacI SacII SexAI SfiI SrfI SwaI