Restriction map for
pFN21AB4221
(Circular) (Six-base) MAPSORT of: pFN21AB4221.seq Check: 4639 from: 1 to: 6388
pFN21AB4221 6388 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2178 3731 1937
Size: 241 1553 4594
Fragments arranged by size:
4594 1553 241
Acc65I G'GTAC_C
Cuts at: 2117 2812 3709 2117
Size: 695 897 4796
Fragments arranged by size:
4796 897 695
AclI AA'CG_TT
Cuts at: 1563 2280 4515 4888 1563
Size: 717 2235 373 3063
Fragments arranged by size:
3063 2235 717 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 3104 3114 3188 4451 5835 6103 1632
Size: 402 1070 10 74 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1070 402 268 74 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 3626 1166
Size: 2460 3928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3545 3577 3398
Size: 32 115 32 6209
Fragments arranged by size:
6209 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2062 2134 2994 3182 3649 4131 5703 2062
Size: 72 860 188 467 482 1572 2747
Fragments arranged by size:
2747 1572 860 482 467 188 72
ApaI G_GGCC'C
Cuts at: 2674 2696 2674
Size: 22 6366
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 3697 3923 4075 6184 1493
Size: 2204 226 152 2109 1697
Fragments arranged by size:
2204 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2667 2785 3027 3432 3713 1870
Size: 73 724 118 242 405 281 4545
Fragments arranged by size:
4545 724 405 281 242 118 73
AvrII C'CTAG_G
Cuts at: 3683 3683
Size: 6388
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2117 2216 2812 3355
Size: 325 217 627 264 66 99 596 543
Cuts at: 3355 3660 3709 5164 618
Size: 305 49 1455 1842
Fragments arranged by size:
1842 1455 627 596 543 325 305 264
217 99 66 49
BanII G_rGCy'C
Cuts at: 1391 2577 2674 2696 2925 3051 3444 3707 1391
Size: 1186 97 22 229 126 393 263 4072
Fragments arranged by size:
4072 1186 393 263 229 126 97 22
BbeI G_GCGC'C
Cuts at: 2220 2220
Size: 6388
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2526 3367 928
Size: 971 627 841 3949
Fragments arranged by size:
3949 971 841 627
BbvCI CC'TCA_GC
Cuts at: 2259 2757 2259
Size: 498 5890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BfrBI ATG'CAT
Cuts at: 2023 2023
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2581 2972
Size: 107 122 71 631 605 337 572 391
Cuts at: 2972 4999 136
Size: 2027 1525
Fragments arranged by size:
2027 1525 631 605 572 391 337 122
107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 2796 3805 2796
Size: 1009 5379
Bme1580I G_kGCm'C
Cuts at: 1790 2056 2634 2674 2696 2786 3141 4449 5605
Size: 266 578 40 22 90 355 1308 1156
Cuts at: 5605 1790
Size: 2573
Fragments arranged by size:
2573 1308 1156 578 355 266 90 40
22
BmgBI CAC'GTC
Cuts at: 1318 2271 1318
Size: 953 5435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2621 3687 4119
Size: 416 53 83 399 24 1204 1066 432
Cuts at: 4119 5077 442
Size: 958 1753
Fragments arranged by size:
1753 1204 1066 958 432 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2581 3571 2563
Size: 18 990 5380
Fragments arranged by size:
5380 990 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 2576 2971 3227 3590 5048 1239
Size: 1183 154 395 256 363 1458 2579
Fragments arranged by size:
2579 1458 1183 395 363 256 154
Bpu10I CC'TnA_GC
Cuts at: 2259 2724 2757 2259
Size: 465 33 5890
Fragments arranged by size:
5890 465 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2990 4513 5378 5676 2606
Size: 384 1523 865 298 3318
Fragments arranged by size:
3318 1523 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 5051 882
Size: 617 759 2793 2219
Fragments arranged by size:
2793 2219 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 2325 3628 4179 493
Size: 711 1121 1303 551 2702
Fragments arranged by size:
2702 1303 1121 711 551
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 4195 4577 5212
Size: 53 83 186 936 684 1978 382 635
Cuts at: 5212 275
Size: 1451
Fragments arranged by size:
1978 1451 936 684 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2877 3465 4821 5219 5493 5640 6051 2877
Size: 588 1356 398 274 147 411 3214
Fragments arranged by size:
3214 1356 588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 2859 2889 1178
Size: 30 165 30 852 30 574 30 4677
Fragments arranged by size:
4677 852 574 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2332 2363 2366 3463 1186
Size: 1090 56 31 3 1097 4111
Fragments arranged by size:
4111 1097 1090 56 31 3
BseYI C'CCAG_C
Cuts at: 2121 2912 3635 5591 6087 2121
Size: 791 723 1956 496 2422
Fragments arranged by size:
2422 1956 791 723 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 5464
Fragments arranged by size:
5464 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2532 2855 3615 4449 4534 5605 2532
Size: 323 760 834 85 1071 3315
Fragments arranged by size:
3315 1071 834 760 323 85
BsmI GAATG_Cn'
Cuts at: 3899 3992 3899
Size: 93 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2532 2577 2634 2674 2696 2786
Size: 399 266 476 45 57 40 22 90
Cuts at: 2786 2855 2925 3051 3141 3444 3615 3707 4449
Size: 69 70 126 90 303 171 92 742
Cuts at: 4449 4534 5605 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 399 303 266 171
126 92 90 90 85 70 69 57
45 40 22
BspEI T'CCGG_A
Cuts at: 2877 3465 6051 2877
Size: 588 2586 3214
Fragments arranged by size:
3214 2586 588
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2109 2424 2824 3743 844
Size: 1226 39 315 400 919 3489
Fragments arranged by size:
3489 1226 919 400 315 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 4275 1394
Size: 429 653 1799 3507
Fragments arranged by size:
3507 1799 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2608 5032 5219 1700
Size: 110 798 2424 187 2869
Fragments arranged by size:
2869 2424 798 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 3718 4085 4470 4487 5939 6025 6037 6383
Size: 1746 367 385 17 1452 86 12 346
Cuts at: 6383 1972
Size: 1977
Fragments arranged by size:
1977 1746 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3183 3183
Size: 6388
BtgI C'CryG_G
Cuts at: 513 1065 2004 2508 2938 3144 3189 3387 513
Size: 552 939 504 430 206 45 198 3514
Fragments arranged by size:
3514 939 552 504 430 206 198 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2976 3359 3904 4698 4718 2976
Size: 383 545 794 20 4646
Fragments arranged by size:
4646 794 545 383 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2392 2935
Size: 54 1425 50 275 18 255 307 543
Cuts at: 2935 2973 3835 4724 6353 8
Size: 38 862 889 1629 43
Fragments arranged by size:
1629 1425 889 862 543 307 275 255
54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 3090 4318 1353
Size: 657 1080 1228 3423
Fragments arranged by size:
3423 1228 1080 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2422 2576 2971 3104 3114 3188
Size: 393 402 388 154 395 133 10 74
Cuts at: 3188 3227 3590 4451 5048 5835 6103 1239
Size: 39 363 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 597 402 395 393 388
363 268 154 133 74 39 10
EcoO109I rG'GnC_Cy
Cuts at: 2692 2693 2773 2943 3100 3493 3580 2692
Size: 1 80 170 157 393 87 5500
Fragments arranged by size:
5500 393 170 157 87 80 1
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 6388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 6388
FspI TGC'GCA
Cuts at: 2381 4894 2381
Size: 2513 3875
HaeII r_GCGC'y
Cuts at: 1678 2220 5535 1678
Size: 542 3315 2531
Fragments arranged by size:
3315 2531 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2426 2458 2824
Size: 32 135 32 683 32 139 32 366
Cuts at: 2824 2856 3500 3520 3532 3552 5029 5061 5103
Size: 32 644 20 12 20 1477 32 42
Cuts at: 5103 5135 1373
Size: 32 2626
Fragments arranged by size:
2626 1477 683 644 366 139 135 42
32 32 32 32 32 32 32 20
20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3732 3978 669
Size: 988 281 1444 350 246 3079
Fragments arranged by size:
3079 1444 988 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KasI G'GCGC_C
Cuts at: 2216 2216
Size: 6388
KpnI G_GTAC'C
Cuts at: 2121 2816 3713 2121
Size: 695 897 4796
Fragments arranged by size:
4796 897 695
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 5502 5686 1305
Size: 993 3204 184 2007
Fragments arranged by size:
3204 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2087 2394 2937 2975 10
Size: 54 1425 343 255 307 543 38 3423
Fragments arranged by size:
3423 1425 543 343 307 255 54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 3626 4346 4705 4864 518
Size: 648 1369 1091 720 359 159 2042
Fragments arranged by size:
2042 1369 1091 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2131 3804 4481 5629 5874 1606
Size: 519 6 1673 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 677 519 245 6
NarI GG'CG_CC
Cuts at: 2217 2217
Size: 6388
NcoI C'CATG_G
Cuts at: 513 1065 2004 3189 513
Size: 552 939 1185 3712
Fragments arranged by size:
3712 1185 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NsiI A_TGCA'T
Cuts at: 2025 2025
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 2665 3746 5291 1132
Size: 97 1436 1081 1545 2229
Fragments arranged by size:
2229 1545 1436 1081 97
PasI CC'CwG_GG
Cuts at: 1348 2808 1348
Size: 1460 4928
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2744 1108
Size: 1636 4752
PfoI T'CCnGG_A
Cuts at: 2895 2895
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 2325 3628 2325
Size: 1303 5085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2773 3100 3493 3580 2773
Size: 327 393 87 5581
Fragments arranged by size:
5581 393 327 87
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PspOMI G'GGCC_C
Cuts at: 2670 2692 2670
Size: 22 6366
PstI C_TGCA'G
Cuts at: 830 2130 3740 830
Size: 1300 1610 3478
Fragments arranged by size:
3478 1610 1300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2125 2131 2125
Size: 6 6382
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SanDI GG'GwC_CC
Cuts at: 2773 2773
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2126 3736 4871 5552 5743 826
Size: 219 1081 1610 1135 681 191 1471
Fragments arranged by size:
1610 1471 1135 1081 681 219 191
SfoI GGC'GCC
Cuts at: 2218 2218
Size: 6388
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 2669 3434 3715 1872
Size: 797 765 281 4545
Fragments arranged by size:
4545 797 765 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3005 4492 5393 5655 820
Size: 197 926 642 420 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 642 420 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2421 2688 3107 3189 3683
Size: 552 939 84 333 267 419 82 494
Cuts at: 3683 3827 513
Size: 144 3074
Fragments arranged by size:
3074 939 552 494 419 333 267 144
84 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2619 3052 3361 3638 4415 4432 4585
Size: 1255 1039 433 309 277 777 17 153
Cuts at: 4585 4770 5215 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1039 777 445 433 309 277
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4634 6346 96
Size: 274 80 33 51 1443 2657 1712 138
Fragments arranged by size:
2657 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 2859 2891 3120 3152 6348 6380 2765
Size: 32 62 32 229 32 3196 32 2773
Fragments arranged by size:
3196 2773 229 62 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2269 1316
Size: 953 5435
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 2667 3432 3713 1870
Size: 797 765 281 4545
Fragments arranged by size:
4545 797 765 281
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI
PacI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI XcmI