Restriction map for
pFN21AB4218
(Circular) (Six-base) MAPSORT of: pFN21AB4218.seq Check: 4906 from: 1 to: 7099
pFN21AB4218 7099 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3504 3504
Size: 7099
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3336 4909 278
Size: 53 83 186 936 1800 1573 2468
Fragments arranged by size:
2468 1800 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4068 4442 1937
Size: 2131 374 4594
Fragments arranged by size:
4594 2131 374
Acc65I G'GTAC_C
Cuts at: 2438 4420 2438
Size: 1982 5117
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4325 5162 6546 6814 1632
Size: 2693 837 1384 268 1917
Fragments arranged by size:
2693 1917 1384 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6902
AflIII A'CryG_T
Cuts at: 1203 5998 6975 1203
Size: 4795 977 1327
Fragments arranged by size:
4795 1327 977
AgeI A'CCGG_T
Cuts at: 5930 5930
Size: 7099
AhdI GACnn_n'nnGTC
Cuts at: 3613 5828 3613
Size: 2215 4884
AleI CACnn'nnGTG
Cuts at: 1166 2645 1166
Size: 1479 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7099
AlwNI CAG_nnn'CTG
Cuts at: 2087 4842 6414 2087
Size: 2755 1572 2772
Fragments arranged by size:
2772 2755 1572
ApaLI G'TGCA_C
Cuts at: 5156 6312 5156
Size: 1156 5943
ApoI r'AATT_y
Cuts at: 1493 2251 2547 3069 4298 4408 4634 4786 6895
Size: 758 296 522 1229 110 226 152 2109
Cuts at: 6895 1493
Size: 1697
Fragments arranged by size:
2109 1697 1229 758 522 296 226 152
110
AseI AT'TA_AT
Cuts at: 160 1032 2886 5653 160
Size: 872 1854 2767 1606
Fragments arranged by size:
2767 1854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2196 2759 3735 3790 3839 4187
Size: 73 133 120 563 976 55 49 348
Cuts at: 4187 4424 1870
Size: 237 4545
Fragments arranged by size:
4545 976 563 348 237 133 120 73
55 49
AvrII C'CTAG_G
Cuts at: 2158 2158
Size: 7099
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2430 2463 2430
Size: 33 7066
BamHI G'GATC_C
Cuts at: 2657 4429 4796 2657
Size: 1772 367 4960
Fragments arranged by size:
4960 1772 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 3446 3492 3599 3730
Size: 325 217 627 651 1008 46 107 131
Cuts at: 3730 4183 4226 4420 5875 618
Size: 453 43 194 1455 1842
Fragments arranged by size:
1842 1455 1008 651 627 453 325 217
194 131 107 46 43
BanII G_rGCy'C
Cuts at: 1391 2307 3840 4418 1391
Size: 916 1533 578 4072
Fragments arranged by size:
4072 1533 916 578
BbeI G_GCGC'C
Cuts at: 3603 3603
Size: 7099
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2175 928
Size: 971 276 5852
Fragments arranged by size:
5852 971 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4071 4105 5290 5324 1903
Size: 34 2134 34 1185 34 3678
Fragments arranged by size:
3678 2134 1185 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4991 6201 3122
Size: 1869 1210 4020
Fragments arranged by size:
4020 1869 1210
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 6261
BfrBI ATG'CAT
Cuts at: 2855 2855
Size: 7099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5710 136
Size: 107 122 71 631 605 4038 1525
Fragments arranged by size:
4038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7094 7094
Size: 7099
BlpI GC'TnA_GC
Cuts at: 3893 4516 3893
Size: 623 6476
Bme1580I G_kGCm'C
Cuts at: 1790 2023 3735 4188 5160 6316 1790
Size: 233 1712 453 972 1156 2573
Fragments arranged by size:
2573 1712 1156 972 453 233
BmgBI CAC'GTC
Cuts at: 1318 4219 1318
Size: 2901 4198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 4830 5788
Size: 416 53 83 399 24 839 2574 958
Cuts at: 5788 442
Size: 1753
Fragments arranged by size:
2574 1753 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 3100 2039
Size: 1061 6038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3150 5759 1239
Size: 1911 2609 2579
Fragments arranged by size:
2609 2579 1911
Bpu10I CC'TnA_GC
Cuts at: 2802 2802
Size: 7099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2460 3809 5224 6089 6387 2460
Size: 1349 1415 865 298 3172
Fragments arranged by size:
3172 1415 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 3520 5762 882
Size: 617 1584 437 2242 2219
Fragments arranged by size:
2242 2219 1584 617 437
BsaAI yAC'GTr
Cuts at: 493 1204 2189 3982 4890 493
Size: 711 985 1793 908 2702
Fragments arranged by size:
2702 1793 985 908 711
BsaBI GATnn'nnATC
Cuts at: 7093 7093
Size: 7099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3333 3600 3663 4906
Size: 53 83 186 936 1800 267 63 1243
Cuts at: 4906 5288 5923 275
Size: 382 635 1451
Fragments arranged by size:
1800 1451 1243 936 635 382 267 186
83 63 53
BsaWI w'CCGG_w
Cuts at: 5532 5930 6204 6351 6762 5532
Size: 398 274 147 411 5869
Fragments arranged by size:
5869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 3572 3602 3986
Size: 30 165 30 762 30 1377 30 384
Cuts at: 3986 4016 1178
Size: 30 4261
Fragments arranged by size:
4261 1377 762 384 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 3945 4165 1186
Size: 1003 1756 220 4120
Fragments arranged by size:
4120 1756 1003 220
BseYI C'CCAG_C
Cuts at: 2065 2164 4005 6302 6798 2065
Size: 99 1841 2297 496 2366
Fragments arranged by size:
2366 2297 1841 496 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 3392 3703 3962 4165 1163
Size: 573 343 1313 311 259 203 4097
Fragments arranged by size:
4097 1313 573 343 311 259 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3744 4549 5310 6338 1540
Size: 275 186 1743 805 761 1028 2301
Fragments arranged by size:
2301 1743 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2476 3987 4122 5160 5245 6316 2476
Size: 1511 135 1038 85 1071 3259
Fragments arranged by size:
3259 1511 1071 1038 135 85
BsmI GAATG_Cn'
Cuts at: 3085 4610 4703 3085
Size: 1525 93 5481
Fragments arranged by size:
5481 1525 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2274 2274
Size: 7099
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2307 2476 3735 3840 3987 4122
Size: 399 233 284 169 1259 105 147 135
Cuts at: 4122 4188 4418 5160 5245 6316 1391
Size: 66 230 742 85 1071 2174
Fragments arranged by size:
2174 1259 1071 742 399 284 233 230
169 147 135 105 85 66
BspEI T'CCGG_A
Cuts at: 6762 6762
Size: 7099
BspHI T'CATG_A
Cuts at: 4333 4988 6718 4333
Size: 655 1730 4714
Fragments arranged by size:
4714 1730 655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 3504 4023 4454 844
Size: 1228 1432 519 431 3489
Fragments arranged by size:
3489 1432 1228 519 431
BsrBI CCG'CTC
Cuts at: 1394 1823 4021 4224 4986 1394
Size: 429 2198 203 762 3507
Fragments arranged by size:
3507 2198 762 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 5594 5768 65
Size: 1183 4346 174 1396
Fragments arranged by size:
4346 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3489 5743 5930 1700
Size: 110 1679 2254 187 2869
Fragments arranged by size:
2869 2254 1679 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7099
BssSI C'ACGA_G
Cuts at: 5159 6171 5159
Size: 1012 6087
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7099
BstBI TT'CG_AA
Cuts at: 4372 5903 4372
Size: 1531 5568
BstEII G'GTnAC_C
Cuts at: 1184 3760 1184
Size: 2576 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 3128 3772 3128
Size: 644 6455
BstYI r'GATC_y
Cuts at: 1972 2657 2806 4429 4796 5181 5198 6650 6736
Size: 685 149 1623 367 385 17 1452 86
Cuts at: 6736 6748 7094 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1623 1452 685 385 367 346 149
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3551 4026 3551
Size: 475 6624
BtgI C'CryG_G
Cuts at: 513 1065 2004 3610 3831 513
Size: 552 939 1606 221 3781
Fragments arranged by size:
3781 1606 939 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 4615 5409 5429 2652
Size: 1963 794 20 4322
Fragments arranged by size:
4322 1963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7064
DraI TTT'AAA
Cuts at: 4403 4403
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3052 6106 809
Size: 507 1736 3054 1802
Fragments arranged by size:
3054 1802 1736 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3909
Size: 54 1425 50 275 18 1705 206 168
Cuts at: 3909 3963 4546 5435 7064 8
Size: 54 583 889 1629 43
Fragments arranged by size:
1705 1629 1425 889 583 275 206 168
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3741 4546 1537
Size: 275 1929 805 4090
Fragments arranged by size:
4090 1929 805 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4109 4115 5029 1353
Size: 2756 6 914 3423
Fragments arranged by size:
3423 2756 914 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5686 6060 6206 387
Size: 1064 138 4097 374 146 1280
Fragments arranged by size:
4097 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3150 4325 5162 5759 6546 6814 1239
Size: 393 1518 1175 837 597 787 268 1524
Fragments arranged by size:
1524 1518 1175 837 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2155 3571 3615 4193 4233 2155
Size: 1416 44 578 40 5021
Fragments arranged by size:
5021 1416 578 44 40
EcoRI G'AATT_C
Cuts at: 3069 4408 4786 3069
Size: 1339 378 5382
Fragments arranged by size:
5382 1339 378
FspI TGC'GCA
Cuts at: 3878 5605 3878
Size: 1727 5372
FspAI rTGC'GCAy
Cuts at: 3878 3878
Size: 7099
HaeII r_GCGC'y
Cuts at: 1678 3429 3603 3641 6246 1678
Size: 1751 174 38 2605 2531
Fragments arranged by size:
2605 2531 1751 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2622 2642 2654
Size: 32 135 32 593 32 425 20 12
Cuts at: 2654 2674 3110 3142 3425 3457 3599 3631 3773
Size: 20 436 32 283 32 142 32 142
Cuts at: 3773 3805 3986 4018 5740 5772 5814 5846 1373
Size: 32 181 32 1722 32 42 32 2626
Fragments arranged by size:
2626 1722 593 436 425 283 181 142
142 135 42 32 32 32 32 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3463 4443 4689 669
Size: 988 281 1525 980 246 3079
Fragments arranged by size:
3079 1525 988 980 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7099
HpaI GTT'AAC
Cuts at: 4689 4689
Size: 7099
KasI G'GCGC_C
Cuts at: 3599 3599
Size: 7099
KpnI G_GTAC'C
Cuts at: 2442 4424 2442
Size: 1982 5117
MfeI C'AATT_G
Cuts at: 4698 4698
Size: 7099
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2428 2470 2682 6213 6397 1305
Size: 1123 42 212 3531 184 2007
Fragments arranged by size:
3531 2007 1123 212 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3537 10
Size: 54 1425 343 1705 3572
Fragments arranged by size:
3572 1705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2696 3317 5057 5416 5575 518
Size: 648 1479 51 621 1740 359 159 2042
Fragments arranged by size:
2042 1740 1479 648 621 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 3612 3697 3820 4515 5192 6340 6585 1606
Size: 2006 85 123 695 677 1148 245 2120
Fragments arranged by size:
2120 2006 1148 695 677 245 123 85
NarI GG'CG_CC
Cuts at: 3600 3600
Size: 7099
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5608
Fragments arranged by size:
5608 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7099
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NsiI A_TGCA'T
Cuts at: 2857 2857
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 4457 6002 1132
Size: 97 3228 1545 2229
Fragments arranged by size:
3228 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7099
PciI A'CATG_T
Cuts at: 5998 5998
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2897 1108
Size: 1789 5310
PfoI T'CCnGG_A
Cuts at: 2780 4280 2780
Size: 1500 5599
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PmlI CAC'GTG
Cuts at: 2189 3982 2189
Size: 1793 5306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5168 5200 6681 6713 5168
Size: 32 1481 32 5554
Fragments arranged by size:
5554 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 3615 4193 4233 2155
Size: 1460 578 40 5021
Fragments arranged by size:
5021 1460 578 40
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7099
PsiI TTA'TAA
Cuts at: 4669 4669
Size: 7099
PstI C_TGCA'G
Cuts at: 830 3702 4451 830
Size: 2872 749 3478
Fragments arranged by size:
3478 2872 749
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7099
PvuII CAG'CTG
Cuts at: 3820 3820
Size: 7099
SacII CC_GC'GG
Cuts at: 3613 3613
Size: 7099
SalI G'TCGA_C
Cuts at: 1936 4441 1936
Size: 2505 4594
SanDI GG'GwC_CC
Cuts at: 3615 4193 4233 3615
Size: 578 40 6481
Fragments arranged by size:
6481 578 40
SapI GCTCTTCn'nnn_
Cuts at: 4109 4109
Size: 7099
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 5347 7059 5347
Size: 1712 5387
SexAI A'CCwGG_T
Cuts at: 3756 4071 3756
Size: 315 6784
SfcI C'TryA_G
Cuts at: 826 1045 3575 3698 4447 5582 6263 6454 826
Size: 219 2530 123 749 1135 681 191 1471
Fragments arranged by size:
2530 1471 1135 749 681 219 191 123
SfoI GGC'GCC
Cuts at: 3601 3601
Size: 7099
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 2198 3737 3841 4426 1872
Size: 326 1539 104 585 4545
Fragments arranged by size:
4545 1539 585 326 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2475 3824 5203 6104 6366 820
Size: 197 926 532 1349 1379 901 262 1553
Fragments arranged by size:
1553 1379 1349 926 901 532 262 197
SnaBI TAC'GTA
Cuts at: 493 4890 493
Size: 4397 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7099
SphI G_CATG'C
Cuts at: 1132 4457 1132
Size: 3325 3774
SrfI GCCC'GGGC
Cuts at: 3841 3841
Size: 7099
SspI AAT'ATT
Cuts at: 5 52 2052 2959 5023 5
Size: 47 2000 907 2064 2081
Fragments arranged by size:
2081 2064 2000 907 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7099
StyI C'CwwG_G
Cuts at: 513 1065 2004 2158 2773 3510 3765 4538 513
Size: 552 939 154 615 737 255 773 3074
Fragments arranged by size:
3074 939 773 737 615 552 255 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2292 3304 3374 5126 5143 5296 5481
Size: 1255 712 1012 70 1752 17 153 185
Cuts at: 5481 5926 325
Size: 445 1498
Fragments arranged by size:
1752 1498 1255 1012 712 445 185 153
70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 3306 5345
Size: 274 80 33 51 1443 1275 54 2039
Cuts at: 5345 7057 96
Size: 1712 138
Fragments arranged by size:
2039 1712 1443 1275 274 138 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7059 7091 7059
Size: 32 7067
Tth111I GACn'n_nGTC
Cuts at: 1316 4237 1316
Size: 2921 4178
XbaI T'CTAG_A
Cuts at: 2404 4435 2404
Size: 2031 5068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3919 3919
Size: 7099
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 2196 3735 3839 4424 1870
Size: 326 1539 104 585 4545
Fragments arranged by size:
4545 1539 585 326 104
XmnI GAAnn'nnTTC
Cuts at: 2865 3203 5228 2865
Size: 338 2025 4736
Fragments arranged by size:
4736 2025 338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3334 4907 276
Size: 53 83 186 936 1800 1573 2468
Fragments arranged by size:
2468 1800 1573 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbvCI BsiWI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SfiI SwaI