Restriction map for
pFN21AB4189
(Circular) (Six-base) MAPSORT of: pFN21AB4189.seq Check: 2596 from: 1 to: 6970
pFN21AB4189 6970 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4780 278
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 4313 1937
Size: 472 1904 4594
Fragments arranged by size:
4594 1904 472
Acc65I G'GTAC_C
Cuts at: 4291 4291
Size: 6970
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2684 3399 3426 3480 3507 3588 3612
Size: 585 467 715 27 54 27 81 24
Cuts at: 3612 3696 5033 6417 6685 1632
Size: 84 1337 1384 268 1917
Fragments arranged by size:
1917 1384 1337 715 585 467 268 84
81 54 27 27 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6773
AflIII A'CryG_T
Cuts at: 1203 2412 2638 5869 6846 1203
Size: 1209 226 3231 977 1327
Fragments arranged by size:
3231 1327 1209 977 226
AgeI A'CCGG_T
Cuts at: 5801 5801
Size: 6970
AhdI GACnn_n'nnGTC
Cuts at: 5699 5699
Size: 6970
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 3164 4713 6285 3164
Size: 1549 1572 3849
Fragments arranged by size:
3849 1572 1549
ApaI G_GGCC'C
Cuts at: 4215 4215
Size: 6970
ApaLI G'TGCA_C
Cuts at: 5027 6183 5027
Size: 1156 5814
ApoI r'AATT_y
Cuts at: 1493 2447 2504 3320 3695 3806 4279 4505 4657
Size: 954 57 816 375 111 473 226 152
Cuts at: 4657 6766 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 954 816 473 375 226 152
111 57
AseI AT'TA_AT
Cuts at: 160 1032 5524 160
Size: 872 4492 1606
Fragments arranged by size:
4492 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 3713 4295 1870
Size: 73 183 1587 582 4545
Fragments arranged by size:
4545 1587 582 183 73
BamHI G'GATC_C
Cuts at: 2965 4300 4667 2965
Size: 1335 367 5268
Fragments arranged by size:
5268 1335 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2931 4291 5746 618
Size: 325 217 627 225 919 1360 1455 1842
Fragments arranged by size:
1842 1455 1360 919 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 4215 4289 1391
Size: 2824 74 4072
Fragments arranged by size:
4072 2824 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 2877 3527 3660 928
Size: 971 498 480 650 133 4238
Fragments arranged by size:
4238 971 650 498 480 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5161 5195 1903
Size: 34 3224 34 3678
Fragments arranged by size:
3678 3224 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3892 4862 6072 3892
Size: 970 1210 4790
Fragments arranged by size:
4790 1210 970
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6970
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5581 136
Size: 107 122 71 631 605 3909 1525
Fragments arranged by size:
3909 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6965 6965
Size: 6970
BlpI GC'TnA_GC
Cuts at: 4387 4387
Size: 6970
Bme1580I G_kGCm'C
Cuts at: 1790 4215 5031 6187 1790
Size: 2425 816 1156 2573
Fragments arranged by size:
2573 2425 1156 816
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6970
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4701 5659 442
Size: 416 53 83 399 24 3284 958 1753
Fragments arranged by size:
3284 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6970
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2508 3094 3865 2508
Size: 586 771 5613
Fragments arranged by size:
5613 771 586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2412 5630 1239
Size: 1173 3218 2579
Fragments arranged by size:
3218 2579 1173
Bpu10I CC'TnA_GC
Cuts at: 3351 3351
Size: 6970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3064 3228 5095 5960 6258 3064
Size: 164 1867 865 298 3776
Fragments arranged by size:
3776 1867 865 298 164
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2746 3716 5633 882
Size: 617 1247 970 1917 2219
Fragments arranged by size:
2219 1917 1247 970 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 4761 493
Size: 711 1209 2348 2702
Fragments arranged by size:
2702 2348 1209 711
BsaBI GATnn'nnATC
Cuts at: 6964 6964
Size: 6970
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4777 5159 5794 275
Size: 53 83 186 936 3244 382 635 1451
Fragments arranged by size:
3244 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5403 5801 6075 6222 6633 5403
Size: 398 274 147 411 5740
Fragments arranged by size:
5740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2131 2161 1178
Size: 30 165 30 728 30 5987
Fragments arranged by size:
5987 728 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3611 3908 4145 1186
Size: 2425 297 237 4011
Fragments arranged by size:
4011 2425 297 237
BseYI C'CCAG_C
Cuts at: 3221 6173 6669 3221
Size: 2952 496 3522
Fragments arranged by size:
3522 2952 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6397
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4420 5181 6209 1540
Size: 275 186 2419 761 1028 2301
Fragments arranged by size:
2419 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3219 5031 5116 6187 3219
Size: 1812 85 1071 4002
Fragments arranged by size:
4002 1812 1071 85
BsmI GAATG_Cn'
Cuts at: 2061 3244 4481 4574 2061
Size: 1183 1237 93 4457
Fragments arranged by size:
4457 1237 1183 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2067 2544 2067
Size: 477 6493
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3219 4215 4289 5031 5116 6187 1391
Size: 399 1429 996 74 742 85 1071 2174
Fragments arranged by size:
2174 1429 1071 996 742 399 85 74
BspEI T'CCGG_A
Cuts at: 6633 6633
Size: 6970
BspHI T'CATG_A
Cuts at: 3754 4859 6589 3754
Size: 1105 1730 4135
Fragments arranged by size:
4135 1730 1105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4325 844
Size: 3481 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 4857 1394
Size: 429 187 2847 3507
Fragments arranged by size:
3507 2847 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 5465 5639 65
Size: 1183 957 3260 174 1396
Fragments arranged by size:
3260 1396 1183 957 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 5614 5801 1700
Size: 110 295 3509 187 2869
Fragments arranged by size:
3509 2869 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6970
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 5030 6042 5030
Size: 1012 5958
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6970
BstBI TT'CG_AA
Cuts at: 2891 4095 5774 2891
Size: 1204 1679 4087
Fragments arranged by size:
4087 1679 1204
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6970
BstYI r'GATC_y
Cuts at: 1972 2488 2965 3482 4300 4667 5052 5069 6521
Size: 516 477 517 818 367 385 17 1452
Cuts at: 6521 6607 6619 6965 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 818 517 516 477 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2410 2410
Size: 6970
Bsu36I CC'TnA_GG
Cuts at: 3132 3149 3132
Size: 17 6953
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4263 487
Size: 683 56 3037 3194
Fragments arranged by size:
3194 3037 683 56
BtsI GCAGTG_nn'
Cuts at: 3030 4486 5280 5300 3030
Size: 1456 794 20 4700
Fragments arranged by size:
4700 1456 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 4274 4274
Size: 6970
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5977 809
Size: 507 4661 1802
Fragments arranged by size:
4661 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 4417 5306
Size: 54 1425 50 275 18 1189 1398 889
Cuts at: 5306 6935 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1398 1189 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4417 1537
Size: 275 2605 4090
Fragments arranged by size:
4090 2605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3402 3483 3537 3588 3885 4020 4900 1353
Size: 2049 81 54 51 297 135 880 3423
Fragments arranged by size:
3423 2049 880 297 135 81 54 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2124 4007 5557 5931 6077 387
Size: 1064 138 535 1883 1550 374 146 1280
Fragments arranged by size:
1883 1550 1280 1064 535 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2412 2684 3399 3426 3480 3507
Size: 393 585 195 272 715 27 54 27
Cuts at: 3507 3588 3612 3696 5033 5630 6417 6685 1239
Size: 81 24 84 1337 597 787 268 1524
Fragments arranged by size:
1524 1337 787 715 597 585 393 272
268 195 84 81 54 27 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3557 3581 3557
Size: 24 6946
EcoO109I rG'GnC_Cy
Cuts at: 2161 3188 4211 4212 2161
Size: 1027 1023 1 4919
Fragments arranged by size:
4919 1027 1023 1
EcoRI G'AATT_C
Cuts at: 4279 4657 4279
Size: 378 6592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 6970
FspI TGC'GCA
Cuts at: 5476 5476
Size: 6970
HaeII r_GCGC'y
Cuts at: 1678 6117 1678
Size: 4439 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 2234 2266 2787
Size: 32 135 32 559 32 71 32 521
Cuts at: 2787 2819 3152 3184 3629 3661 3806 3838 3911
Size: 32 333 32 445 32 145 32 73
Cuts at: 3911 3943 5611 5643 5685 5717 1373
Size: 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 559 521 445 333 145 135
73 71 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4314 4560 669
Size: 988 281 2376 246 3079
Fragments arranged by size:
3079 2376 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6970
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KpnI G_GTAC'C
Cuts at: 4295 4295
Size: 6970
MfeI C'AATT_G
Cuts at: 4569 4569
Size: 6970
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 6084 6268 1305
Size: 1419 3360 184 2007
Fragments arranged by size:
3360 2007 1419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5148
Fragments arranged by size:
5148 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 2937 3759 4928 5287 5446 518
Size: 648 1314 457 822 1169 359 159 2042
Fragments arranged by size:
2042 1314 1169 822 648 457 359 159
MspA1I CmG'CkG
Cuts at: 1606 3441 4386 5063 6211 6456 1606
Size: 1835 945 677 1148 245 2120
Fragments arranged by size:
2120 1835 1148 945 677 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6418
NdeI CA'TA_TG
Cuts at: 387 3904 387
Size: 3517 3453
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6970
NotI GC'GGCC_GC
Cuts at: 4417 4417
Size: 6970
NspI r_CATG'y
Cuts at: 1132 1229 2642 4328 5873 1132
Size: 97 1413 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 1413 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6970
PciI A'CATG_T
Cuts at: 2638 5869 2638
Size: 3231 3739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6970
PfoI T'CCnGG_A
Cuts at: 2777 2777
Size: 6970
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2161
Size: 6970
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6970
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PspOMI G'GGCC_C
Cuts at: 4211 4211
Size: 6970
PstI C_TGCA'G
Cuts at: 830 2296 4322 830
Size: 1466 2026 3478
Fragments arranged by size:
3478 2026 1466
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 3441 3441
Size: 6970
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SbfI CC_TGCA'GG
Cuts at: 4322 4322
Size: 6970
ScaI AGT'ACT
Cuts at: 5218 6930 5218
Size: 1712 5258
SfcI C'TryA_G
Cuts at: 826 1045 2292 3054 3279 4318 5453 6134 6325
Size: 219 1247 762 225 1039 1135 681 191
Cuts at: 6325 826
Size: 1471
Fragments arranged by size:
1471 1247 1135 1039 762 681 225 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 4297 1872
Size: 2425 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3079 3243 3713 5074 5975 6237
Size: 197 926 1136 164 470 1361 901 262
Cuts at: 6237 820
Size: 1553
Fragments arranged by size:
1553 1361 1136 926 901 470 262 197
164
SnaBI TAC'GTA
Cuts at: 493 4761 493
Size: 4268 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SspI AAT'ATT
Cuts at: 5 52 4894 5
Size: 47 4842 2081
Fragments arranged by size:
4842 2081 47
StuI AGG'CCT
Cuts at: 1517 3147 1517
Size: 1630 5340
StyI C'CwwG_G
Cuts at: 513 1065 4409 513
Size: 552 3344 3074
Fragments arranged by size:
3344 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 2114 2949 4175 4997 5014 5167
Size: 1255 528 6 835 1226 822 17 153
Cuts at: 5167 5352 5797 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1226 835 822 528 445 185
153 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5216 6928 96
Size: 274 80 33 51 1443 3239 1712 138
Fragments arranged by size:
3239 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4171 4203 6930 6962 4171
Size: 32 2727 32 4179
Fragments arranged by size:
4179 2727 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6970
XbaI T'CTAG_A
Cuts at: 3194 4306 3194
Size: 1112 5858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3044 3044
Size: 6970
XhoI C'TCGA_G
Cuts at: 1943 3713 1943
Size: 1770 5200
XmaI C'CCGG_G
Cuts at: 1870 4295 1870
Size: 2425 4545
XmnI GAAnn'nnTTC
Cuts at: 2874 3762 5099 2874
Size: 888 1337 4745
Fragments arranged by size:
4745 1337 888
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4778 276
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI