Restriction map for
pFN21AB4188
(Circular) (Six-base) MAPSORT of: pFN21AB4188.seq Check: 7181 from: 1 to: 5776
pFN21AB4188 5776 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2348 3081 3839 5223 5491 1632
Size: 492 224 733 758 1384 268 1917
Fragments arranged by size:
1917 1384 758 733 492 268 224
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3009 1184
Size: 1825 3951
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaI G_GGCC'C
Cuts at: 3050 3050
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2336 3027 3085 3311 3463 5572 1493
Size: 843 691 58 226 152 2109 1697
Fragments arranged by size:
2109 1697 843 691 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2574 3101 1870
Size: 73 631 527 4545
Fragments arranged by size:
4545 631 527 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2492 3050 3095 1391
Size: 1101 558 45 4072
Fragments arranged by size:
4072 1101 558 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3040 928
Size: 971 1141 3664
Fragments arranged by size:
3664 1141 971
BbvCI CC'TCA_GC
Cuts at: 2454 2454
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2801 3668 4878 2801
Size: 867 1210 3699
Fragments arranged by size:
3699 1210 867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4387 136
Size: 107 122 71 631 605 916 1799 1525
Fragments arranged by size:
1799 1525 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2049 5771 2049
Size: 3722 2054
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 3050 3837 4993 1790
Size: 1260 787 1156 2573
Fragments arranged by size:
2573 1260 1156 787
BmgBI CAC'GTC
Cuts at: 1318 2521 1318
Size: 1203 4573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 4436 1239
Size: 1017 2180 2579
Fragments arranged by size:
2579 2180 1017
Bpu10I CC'TnA_GC
Cuts at: 2454 2454
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2274 4439 882
Size: 617 775 2165 2219
Fragments arranged by size:
2219 2165 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 2477 5770 2477
Size: 3293 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 3049 4979 5475 3049
Size: 1930 496 3350
Fragments arranged by size:
3350 1930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 1163
Size: 573 912 4291
Fragments arranged by size:
4291 912 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 2239 3287 3380 2239
Size: 1048 93 4635
Fragments arranged by size:
4635 1048 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2492 3050 3095 3837 3922 4993 1391
Size: 399 702 558 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 558 399 85 45
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 3663 1394
Size: 429 1007 833 3507
Fragments arranged by size:
3507 1007 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2259 4271 4445 65
Size: 1183 1011 2012 174 1396
Fragments arranged by size:
2012 1396 1183 1011 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 4420 4607 1700
Size: 110 208 2402 187 2869
Fragments arranged by size:
2869 2402 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 3031 4580 3031
Size: 1549 4227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2049 3106 3473 3858 3875 5327 5413 5425
Size: 77 1057 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1057 385 367 346 86 77
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 2667 3223
Size: 54 1425 50 275 18 517 320 556
Cuts at: 3223 4112 5741 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 556 517 320 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2256 2348 3081 3839 4436 5223
Size: 393 492 132 92 733 758 597 787
Cuts at: 5223 5491 1239
Size: 268 1524
Fragments arranged by size:
1524 787 758 733 597 492 393 268
132 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2186
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 3008 3046 3008
Size: 38 5738
EcoRI G'AATT_C
Cuts at: 3027 3085 3463 3027
Size: 58 378 5340
Fragments arranged by size:
5340 378 58
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3120 3366 669
Size: 988 281 996 186 246 3079
Fragments arranged by size:
3079 996 988 281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 2613 4890 5074 1305
Size: 861 447 2277 184 2007
Fragments arranged by size:
2277 2007 861 447 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 2669 10
Size: 54 1425 343 517 320 3117
Fragments arranged by size:
3117 1425 517 343 320 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 2757 3734 4093 4252 518
Size: 648 1117 474 977 359 159 2042
Fragments arranged by size:
2042 1117 977 648 474 359 159
MspA1I CmG'CkG
Cuts at: 1606 2453 3053 3192 3869 5017 5262 1606
Size: 847 600 139 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 600 245 139
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2817 2978 3134 4679 1132
Size: 97 1588 161 156 1545 2229
Fragments arranged by size:
2229 1588 1545 161 156 97
PasI CC'CwG_GG
Cuts at: 1348 2239 1348
Size: 891 4885
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3008 3008
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 2660 3346 2660
Size: 686 5090
PspOMI G'GGCC_C
Cuts at: 3046 3046
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2452 2941 3016 3128 830
Size: 1622 489 75 112 3478
Fragments arranged by size:
3478 1622 489 112 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2453 2453
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3016 3128 3016
Size: 112 5664
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2448 2937 3012 3124 4259 4940 5131
Size: 219 1403 489 75 112 1135 681 191
Cuts at: 5131 826
Size: 1471
Fragments arranged by size:
1471 1403 1135 681 489 219 191 112
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2574 3880 4781 5043 820
Size: 197 926 631 1306 901 262 1553
Fragments arranged by size:
1553 1306 926 901 631 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2978 3134 1132
Size: 1846 156 3774
Fragments arranged by size:
3774 1846 156
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2715 3215 513
Size: 552 1650 500 3074
Fragments arranged by size:
3074 1650 552 500
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3803 3820 3973 4158 4603 325
Size: 1255 933 1290 17 153 185 445 1498
Fragments arranged by size:
1498 1290 1255 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 2959 4022
Size: 274 80 33 51 1443 99 883 1063
Cuts at: 4022 5734 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1063 883 274 138 99 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 2574 1943
Size: 631 5145
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 3905 2583
Size: 1322 4454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI