Restriction map for
pFN21AB4185
(Circular) (Six-base) MAPSORT of: pFN21AB4185.seq Check: 8899 from: 1 to: 5860
pFN21AB4185 5860 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2294 3670 278
Size: 53 83 186 936 758 1376 2468
Fragments arranged by size:
2468 1376 936 758 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2094 3203 1937
Size: 157 1109 4594
Fragments arranged by size:
4594 1109 157
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 3923 5307 5575 1632
Size: 617 1674 1384 268 1917
Fragments arranged by size:
1917 1674 1384 617 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2776 3603 5175 2776
Size: 827 1572 3461
Fragments arranged by size:
3461 1572 827
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2855 3169 3395 3547 5656 1493
Size: 1362 314 226 152 2109 1697
Fragments arranged by size:
2109 1697 1362 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 3185 1870
Size: 73 131 1111 4545
Fragments arranged by size:
4545 1111 131 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3181 4636 618
Size: 325 217 627 1394 1455 1842
Fragments arranged by size:
1842 1455 1394 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3179 1391
Size: 1788 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BfrBI ATG'CAT
Cuts at: 2874 2874
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 2484 2967 3277 2484
Size: 483 310 5067
Fragments arranged by size:
5067 483 310
Bme1580I G_kGCm'C
Cuts at: 1790 3921 5077 1790
Size: 2131 1156 2573
Fragments arranged by size:
2573 2131 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2499 2499
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 2439 2450 4520 1239
Size: 1196 4 11 2070 2579
Fragments arranged by size:
2579 2070 1196 11 4
Bpu10I CC'TnA_GC
Cuts at: 2113 2113
Size: 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 2291 3667 4049
Size: 53 83 186 936 729 29 1376 382
Cuts at: 4049 4684 275
Size: 635 1451
Fragments arranged by size:
1451 1376 936 729 635 382 186 83
53 29
BsaWI w'CCGG_w
Cuts at: 2185 2710 2838 2928 4293 4691 4965 5112 5523
Size: 525 128 90 1365 398 274 147 411
Cuts at: 5523 2185
Size: 2522
Fragments arranged by size:
2522 1365 525 411 398 274 147 128
90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 4465
Fragments arranged by size:
4465 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 1186
Size: 1168 4692
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 2288 1163
Size: 573 497 55 4735
Fragments arranged by size:
4735 573 497 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 3310 4071 5099 1540
Size: 275 186 67 1242 761 1028 2301
Fragments arranged by size:
2301 1242 1028 761 275 186 67
BsiHKAI G_wGCw'C
Cuts at: 3921 4006 5077 3921
Size: 85 1071 4704
Fragments arranged by size:
4704 1071 85
BsmI GAATG_Cn'
Cuts at: 2938 3371 3464 2938
Size: 433 93 5334
Fragments arranged by size:
5334 433 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3179 3921 4006 5077 1391
Size: 399 1389 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2928 5523 2928
Size: 2595 3265
BspHI T'CATG_A
Cuts at: 2586 3749 5479 2586
Size: 1163 1730 2967
Fragments arranged by size:
2967 1730 1163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 2461 3747 1394
Size: 429 455 183 1286 3507
Fragments arranged by size:
3507 1286 455 429 183
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 3115 4355 4529 65
Size: 1183 1695 172 1240 174 1396
Fragments arranged by size:
1695 1396 1240 1183 174 172
BsrFI r'CCGG_y
Cuts at: 1700 1810 2386 4504 4691 1700
Size: 110 576 2118 187 2869
Fragments arranged by size:
2869 2118 576 187 110
BsrGI T'GTAC_A
Cuts at: 96 3141 96
Size: 3045 2815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 2273 2471 3920 4932 2273
Size: 198 1449 1012 3201
Fragments arranged by size:
3201 1449 1012 198
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855
Size: 1218 367 385 17 1452 86 12 346
Cuts at: 5855 1972
Size: 1977
Fragments arranged by size:
1977 1452 1218 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2326 2400 2439 2547 513
Size: 552 1261 74 39 108 3826
Fragments arranged by size:
3826 1261 552 108 74 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2041 2076 529
Size: 35 1477 35 4313
Fragments arranged by size:
4313 1477 35 35
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2906 2906
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2544 3307
Size: 54 1425 50 275 18 606 108 763
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 763 606 275 108 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 3790 1353
Size: 1235 1202 3423
Fragments arranged by size:
3423 1235 1202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2435 2439 2450 3923 4520 5307
Size: 393 617 186 4 11 1473 597 787
Cuts at: 5307 5575 1239
Size: 268 1524
Fragments arranged by size:
1524 1473 787 617 597 393 268 186
11 4
EcoO109I rG'GnC_Cy
Cuts at: 2148 2282 2520 2148
Size: 134 238 5488
Fragments arranged by size:
5488 238 134
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 2044 3002 3157 2044
Size: 958 155 4747
Fragments arranged by size:
4747 958 155
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2378 5007 1678
Size: 700 2629 2531
Fragments arranged by size:
2629 2531 700
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2096 2128 4501
Size: 32 135 32 485 32 7 32 2373
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2373 485 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2248 3204 3450 669
Size: 988 281 310 956 246 3079
Fragments arranged by size:
3079 988 956 310 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2546 10
Size: 54 1425 343 714 3324
Fragments arranged by size:
3324 1425 714 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4177 4336 518
Size: 648 2652 359 159 2042
Fragments arranged by size:
2652 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2413 2441 2570 2647 3276 3953 5101
Size: 796 11 28 129 77 629 677 1148
Cuts at: 5101 5346 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 796 677 629 245 129 77
28 11
NaeI GCC'GGC
Cuts at: 2388 2388
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2386 2386
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NsiI A_TGCA'T
Cuts at: 2876 2876
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2520 2148
Size: 372 5488
PshAI GACnn'nnGTC
Cuts at: 1613 2153 1613
Size: 540 5320
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2413 2570 2647 2413
Size: 157 77 5626
Fragments arranged by size:
5626 157 77
SacII CC_GC'GG
Cuts at: 2403 2442 2403
Size: 39 5821
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SanDI GG'GwC_CC
Cuts at: 2520 2520
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 3075 3208 4343 5024 5215 826
Size: 219 2030 133 1135 681 191 1471
Fragments arranged by size:
2030 1471 1135 681 219 191 133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 3299 513
Size: 552 2234 3074
Fragments arranged by size:
3074 2234 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3141 4106 5818
Size: 274 80 33 51 1443 1164 965 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1712 1443 1164 965 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 5820 5852 2543
Size: 32 3245 32 2551
Fragments arranged by size:
3245 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2513 2513
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2292 3668 276
Size: 53 83 186 936 758 1376 2468
Fragments arranged by size:
2468 1376 936 758 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI