Restriction map for
pFN21AB4176
(Circular) (Six-base) MAPSORT of: pFN21AB4176.seq Check: 3724 from: 1 to: 6058
pFN21AB4176 6058 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2471 3401 1937
Size: 534 930 4594
Fragments arranged by size:
4594 930 534
Acc65I G'GTAC_C
Cuts at: 3293 3379 3293
Size: 86 5972
AclI AA'CG_TT
Cuts at: 1563 2305 4185 4558 1563
Size: 742 1880 373 3063
Fragments arranged by size:
3063 1880 742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2562 3240 4121 5505 5773 1632
Size: 591 339 678 881 1384 268 1917
Fragments arranged by size:
1917 1384 881 678 591 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 3262 4787 3262
Size: 1525 4533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2041 3113 3801 5373 2041
Size: 1072 688 1572 2726
Fragments arranged by size:
2726 1572 1072 688
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2136 2312 2445 2552 2908 2993 3367 3593
Size: 643 176 133 107 356 85 374 226
Cuts at: 3593 3745 5854 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 643 374 356 226 176 152
133 107 85
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 1870
Size: 73 1440 4545
Fragments arranged by size:
4545 1440 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3293 3379 4834 618
Size: 325 217 627 1506 86 1455 1842
Fragments arranged by size:
1842 1506 1455 627 325 217 86
BanII G_rGCy'C
Cuts at: 1391 3377 1391
Size: 1986 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2200 928
Size: 971 116 185 4786
Fragments arranged by size:
4786 971 185 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BfrBI ATG'CAT
Cuts at: 2567 2609 2969 2567
Size: 42 360 5656
Fragments arranged by size:
5656 360 42
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 4119 5275 1790
Size: 2329 1156 2573
Fragments arranged by size:
2573 2329 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 3322 3789
Size: 416 53 83 399 24 778 1127 467
Cuts at: 3789 4747 442
Size: 958 1753
Fragments arranged by size:
1753 1127 958 778 467 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2361 1056
Size: 1305 4753
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 4718 1239
Size: 1119 2360 2579
Fragments arranged by size:
2579 2360 1119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4721 882
Size: 617 3222 2219
Fragments arranged by size:
3222 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3849 493
Size: 711 1020 1625 2702
Fragments arranged by size:
2702 1625 1020 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6058
BseYI C'CCAG_C
Cuts at: 2665 5261 5757 2665
Size: 2596 496 2966
Fragments arranged by size:
2966 2596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2905 4119 4204 5275 2905
Size: 1214 85 1071 3688
Fragments arranged by size:
3688 1214 1071 85
BsmI GAATG_Cn'
Cuts at: 2504 3569 3662 2504
Size: 1065 93 4900
Fragments arranged by size:
4900 1065 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2905 3377 4119 4204 5275 1391
Size: 399 1115 472 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 742 472 399 85
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 2790 3947 5677 2790
Size: 1157 1730 3171
Fragments arranged by size:
3171 1730 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 3413 844
Size: 1826 743 3489
Fragments arranged by size:
3489 1826 743
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 794
Size: 1247 4811
BstBI TT'CG_AA
Cuts at: 2443 4862 2443
Size: 2419 3639
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 3145 3388 3755 4140 4157 5609 5695 5707
Size: 1173 243 367 385 17 1452 86 12
Cuts at: 5707 6053 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1173 385 367 346 243 86
17 12
BstZ17I GTA'TAC
Cuts at: 2472 2472
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3631
Fragments arranged by size:
3631 1875 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3114 3574 4368 4388 3114
Size: 460 794 20 4784
Fragments arranged by size:
4784 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 2310 2317 3327 3362 2310
Size: 7 1010 35 5006
Fragments arranged by size:
5006 1010 35 7
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3505 4394 6023
Size: 54 1425 50 275 18 1675 889 1629
Cuts at: 6023 8
Size: 43
Fragments arranged by size:
1675 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3038 3988 1353
Size: 1685 950 3423
Fragments arranged by size:
3423 1685 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2358 2562 3240 4121 4718 5505
Size: 393 591 135 204 678 881 597 787
Cuts at: 5505 5773 1239
Size: 268 1524
Fragments arranged by size:
1524 881 787 678 597 591 393 268
204 135
EcoNI CCTnn'n_nnAGG
Cuts at: 3342 3342
Size: 6058
EcoO109I rG'GnC_Cy
Cuts at: 2065 2321 2065
Size: 256 5802
EcoRI G'AATT_C
Cuts at: 2136 2445 2908 3367 3745 2136
Size: 309 463 459 378 4449
Fragments arranged by size:
4449 463 459 378 309
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3248 3280 4699 4731 4773
Size: 32 135 32 1676 32 1419 32 42
Cuts at: 4773 4805 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1419 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3297 3383 3297
Size: 86 5972
MfeI C'AATT_G
Cuts at: 2268 3657 2268
Size: 1389 4669
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5172 5356 1305
Size: 3867 184 2007
Fragments arranged by size:
3867 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4236
Fragments arranged by size:
4236 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 4016 4375 4534 518
Size: 648 1056 1794 359 159 2042
Fragments arranged by size:
2042 1794 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3218 3474 4151 5299 5544 1606
Size: 1612 256 677 1148 245 2120
Fragments arranged by size:
2120 1612 1148 677 256 245
NcoI C'CATG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3631
Fragments arranged by size:
3631 1875 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 2357 1052
Size: 1305 4753
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NsiI A_TGCA'T
Cuts at: 2569 2611 2971 2569
Size: 42 360 5656
Fragments arranged by size:
5656 360 42
NspI r_CATG'y
Cuts at: 1132 1229 2035 3416 4961 1132
Size: 97 806 1381 1545 2229
Fragments arranged by size:
2229 1545 1381 806 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2946 1108
Size: 1838 4220
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2321 2065
Size: 256 5802
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PstI C_TGCA'G
Cuts at: 830 3410 830
Size: 2580 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 3218 3218
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SanDI GG'GwC_CC
Cuts at: 2065 2065
Size: 6058
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 3406 4541 5222 5413 826
Size: 219 2361 1135 681 191 1471
Fragments arranged by size:
2361 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 3385 1872
Size: 1513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4162 5063 5325 820
Size: 197 926 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2940 3497 513
Size: 552 1875 557 3074
Fragments arranged by size:
3074 1875 557 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 2702 4304
Size: 274 80 33 51 1443 643 82 1602
Cuts at: 4304 6016 96
Size: 1712 138
Fragments arranged by size:
1712 1602 1443 643 274 138 82 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6018 6050 6018
Size: 32 6026
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2080
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 3383 1870
Size: 1513 4545
XmnI GAAnn'nnTTC
Cuts at: 3233 4187 3233
Size: 954 5104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI