Restriction map for
pFN21AB4173
(Circular) (Six-base) MAPSORT of: pFN21AB4173.seq Check: 4095 from: 1 to: 7561
pFN21AB4173 7561 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4381 4381
Size: 7561
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2256 3204 3942 5371
Size: 53 83 186 936 720 948 738 1429
Cuts at: 5371 278
Size: 2468
Fragments arranged by size:
2468 1429 948 936 738 720 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2999 3021 4904 1937
Size: 1062 22 1883 4594
Fragments arranged by size:
4594 1883 1062 22
Acc65I G'GTAC_C
Cuts at: 4882 4882
Size: 7561
AclI AA'CG_TT
Cuts at: 1563 5688 6061 1563
Size: 4125 373 3063
Fragments arranged by size:
4125 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2726 4048 5624 7008 7276 1632
Size: 1094 1322 1576 1384 268 1917
Fragments arranged by size:
1917 1576 1384 1322 1094 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7364
AflIII A'CryG_T
Cuts at: 1203 3852 4675 6460 7437 1203
Size: 2649 823 1785 977 1327
Fragments arranged by size:
2649 1785 1327 977 823
AgeI A'CCGG_T
Cuts at: 6392 6392
Size: 7561
AhdI GACnn_n'nnGTC
Cuts at: 2917 6290 2917
Size: 3373 4188
AleI CACnn'nnGTG
Cuts at: 1166 2708 4265 4658 1166
Size: 1542 1557 393 4069
Fragments arranged by size:
4069 1557 1542 393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7561
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 3233 3265 3554 3586 2414
Size: 32 787 32 289 32 6389
Fragments arranged by size:
6389 787 289 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2353 3067 3295 3430 4237 5304 6876 2353
Size: 714 228 135 807 1067 1572 3038
Fragments arranged by size:
3038 1572 1067 807 714 228 135
ApaI G_GGCC'C
Cuts at: 2199 3038 3187 3356 4490 2199
Size: 839 149 169 1134 5270
Fragments arranged by size:
5270 1134 839 169 149
ApaLI G'TGCA_C
Cuts at: 2669 5618 6774 2669
Size: 2949 1156 3456
Fragments arranged by size:
3456 2949 1156
ApoI r'AATT_y
Cuts at: 1493 4870 5096 5248 7357 1493
Size: 3377 226 152 2109 1697
Fragments arranged by size:
3377 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6115 160
Size: 872 5083 1606
Fragments arranged by size:
5083 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7561
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2199 3349 3449 3774 4563 4886
Size: 73 178 78 1150 100 325 789 323
Cuts at: 4886 1870
Size: 4545
Fragments arranged by size:
4545 1150 789 325 323 178 100 78
73
BamHI G'GATC_C
Cuts at: 3009 3331 4891 5258 3009
Size: 322 1560 367 5312
Fragments arranged by size:
5312 1560 367 322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2581 2614 2713 3532
Size: 325 217 627 229 565 33 99 819
Cuts at: 3532 3919 4336 4738 4882 6337 618
Size: 387 417 402 144 1455 1842
Fragments arranged by size:
1842 1455 819 627 565 417 402 387
325 229 217 144 99 33
BanII G_rGCy'C
Cuts at: 1391 2199 2365 2432 2655 2981 3038 3045 3187
Size: 808 166 67 223 326 57 7 142
Cuts at: 3187 3356 3590 3863 4490 4880 1391
Size: 169 234 273 627 390 4072
Fragments arranged by size:
4072 808 627 390 326 273 234 223
169 166 142 67 57 7
BbeI G_GCGC'C
Cuts at: 2717 4742 2717
Size: 2025 5536
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5752 5786 1903
Size: 34 3815 34 3678
Fragments arranged by size:
3815 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 5453 6663 3074
Size: 2379 1210 3972
Fragments arranged by size:
3972 2379 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3132 4082 4283
Size: 107 122 71 631 605 1460 950 201
Cuts at: 4283 6172 136
Size: 1889 1525
Fragments arranged by size:
1889 1525 1460 950 631 605 201 122
107 71
BglII A'GATC_T
Cuts at: 7556 7556
Size: 7561
BlpI GC'TnA_GC
Cuts at: 2810 4978 2810
Size: 2168 5393
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2199 2241 2586 2617 2673 3038 3187
Size: 301 108 42 345 31 56 365 149
Cuts at: 3187 3356 3537 4316 4490 5622 6778 1790
Size: 169 181 779 174 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 779 365 345 301 181
174 169 149 108 56 42 31
BmgBI CAC'GTC
Cuts at: 1318 2515 3853 4156 1318
Size: 1197 1338 303 4723
Fragments arranged by size:
4723 1338 1197 303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2138 3112 4259
Size: 416 53 83 399 24 721 974 1147
Cuts at: 4259 5292 6250 442
Size: 1033 958 1753
Fragments arranged by size:
1753 1147 1033 974 958 721 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2438 2927 3013 3638 3644 3667 4211
Size: 932 267 489 86 625 6 23 544
Cuts at: 4211 4256 4628 6221 1239
Size: 45 372 1593 2579
Fragments arranged by size:
2579 1593 932 625 544 489 372 267
86 45 23 6
Bpu10I CC'TnA_GC
Cuts at: 2426 2987 2426
Size: 561 7000
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5686 6551 6849 5686
Size: 865 298 6398
Fragments arranged by size:
6398 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 2889 3410 4160 6224 882
Size: 617 1086 304 521 750 2064 2219
Fragments arranged by size:
2219 2064 1086 750 617 521 304
BsaAI yAC'GTr
Cuts at: 493 1204 3634 4676 5352 493
Size: 711 2430 1042 676 2702
Fragments arranged by size:
2702 2430 1042 711 676
BsaBI GATnn'nnATC
Cuts at: 7555 7555
Size: 7561
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 2714 3201 3939
Size: 53 83 186 936 720 461 487 738
Cuts at: 3939 4739 5368 5750 6385 275
Size: 800 629 382 635 1451
Fragments arranged by size:
1451 936 800 738 720 635 629 487
461 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5994 6392 6666 6813 7224 5994
Size: 398 274 147 411 6331
Fragments arranged by size:
6331 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 3215 3245 1178
Size: 30 165 30 1011 30 771 30 5494
Fragments arranged by size:
5494 1011 771 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2168 2422 2663 3130 3871 3983 4109
Size: 942 40 254 241 467 741 112 126
Cuts at: 4109 1186
Size: 4638
Fragments arranged by size:
4638 942 741 467 254 241 126 112
40
BseYI C'CCAG_C
Cuts at: 2164 2396 2405 3129 3913 3990 3994 4077 4463
Size: 232 9 724 784 77 4 83 386
Cuts at: 4463 4743 6764 7260 2164
Size: 280 2021 496 2465
Fragments arranged by size:
2465 2021 784 724 496 386 280 232
83 77 9 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2061 3077 3281 3308 3701 1163
Size: 573 325 1016 204 27 393 5023
Fragments arranged by size:
5023 1016 573 393 325 204 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2224 2689 3310 3826 4659 5011
Size: 275 186 223 465 621 516 833 352
Cuts at: 5011 5772 6800 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 833 761 621 516 465 352
275 223 186
BsiHKAI G_wGCw'C
Cuts at: 2432 2673 2793 3863 4272 5622 5707 6778 2432
Size: 241 120 1070 409 1350 85 1071 3215
Fragments arranged by size:
3215 1350 1071 1070 409 241 120 85
BsmI GAATG_Cn'
Cuts at: 5072 5165 5072
Size: 93 7468
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2199 2241 2365 2432 2586 2617
Size: 399 301 108 42 124 67 154 31
Cuts at: 2617 2655 2673 2793 2981 3038 3045 3187 3356
Size: 38 18 120 188 57 7 142 169
Cuts at: 3356 3537 3590 3863 4272 4316 4490 4880 5622
Size: 181 53 273 409 44 174 390 742
Cuts at: 5622 5707 6778 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 390 301 273
188 181 174 169 154 142 124 120
108 85 67 57 53 44 42 38
31 18 7
BspEI T'CCGG_A
Cuts at: 7224 7224
Size: 7561
BspHI T'CATG_A
Cuts at: 5450 7180 5450
Size: 1730 5831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3723 3918 4335 4381 4738 4916 844
Size: 2879 195 417 46 357 178 3489
Fragments arranged by size:
3489 2879 417 357 195 178 46
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 5448 1394
Size: 429 1981 1644 3507
Fragments arranged by size:
3507 1981 1644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2956 6056 6230 65
Size: 1183 1708 3100 174 1396
Fragments arranged by size:
3100 1708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2673 3309 3419 3833 4072 4426
Size: 110 224 639 636 110 414 239 354
Cuts at: 4426 6205 6392 1700
Size: 1779 187 2869
Fragments arranged by size:
2869 1779 639 636 414 354 239 224
187 110 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7561
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7561
BssSI C'ACGA_G
Cuts at: 2057 5621 6633 2057
Size: 3564 1012 2985
Fragments arranged by size:
3564 2985 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2075 2353 3295 4336 794
Size: 1281 278 942 1041 4019
Fragments arranged by size:
4019 1281 1041 942 278
BstBI TT'CG_AA
Cuts at: 6365 6365
Size: 7561
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7561
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 3052 3490 4531 4807 2962
Size: 90 438 1041 276 5716
Fragments arranged by size:
5716 1041 438 276 90
BstYI r'GATC_y
Cuts at: 1972 2557 3009 3331 4891 5258 5643 5660 7112
Size: 585 452 322 1560 367 385 17 1452
Cuts at: 7112 7198 7210 7556 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1560 1452 585 452 385 367 346
322 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2320 2955 3282 3820 4170 4524
Size: 552 939 316 635 327 538 350 354
Cuts at: 4524 4734 513
Size: 210 3340
Fragments arranged by size:
3340 939 635 552 538 354 350 327
316 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3730 487
Size: 683 56 2504 4318
Fragments arranged by size:
4318 2504 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 3456 4418 5077 5871 5891 2241
Size: 1215 962 659 794 20 3911
Fragments arranged by size:
3911 1215 962 794 659 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7561
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7526
DraI TTT'AAA
Cuts at: 4865 4865
Size: 7561
DraIII CAC_nnn'GTG
Cuts at: 2247 2838 2932 2247
Size: 591 94 6876
Fragments arranged by size:
6876 591 94
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4802 4817 6568 809
Size: 507 3486 15 1751 1802
Fragments arranged by size:
3486 1802 1751 507 15
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2221 2451
Size: 54 1425 50 275 18 213 178 230
Cuts at: 2451 2686 3279 3307 3421 3666 3817 3823 3835
Size: 235 593 28 114 245 151 6 12
Cuts at: 3835 4641 4731 5008 5897 7526 8
Size: 806 90 277 889 1629 43
Fragments arranged by size:
1629 1425 889 806 593 277 275 245
235 230 213 178 151 114 90 54
50 43 28 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 2221 2686 3307 3823 5008 1537
Size: 275 409 465 621 516 1185 4090
Fragments arranged by size:
4090 1185 621 516 465 409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4190 5491 1353
Size: 2837 1301 3423
Fragments arranged by size:
3423 2837 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6148 6522 6668 387
Size: 1064 138 4559 374 146 1280
Fragments arranged by size:
4559 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2430 3861 2430
Size: 1431 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2438 2726 2927 3013 3638 3644
Size: 393 539 267 288 201 86 625 6
Cuts at: 3644 3667 4048 4211 4256 4628 5624 6221 7008
Size: 23 381 163 45 372 996 597 787
Cuts at: 7008 7276 1239
Size: 268 1524
Fragments arranged by size:
1524 996 787 625 597 539 393 381
372 288 268 267 201 163 86 45
23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3754 4547 3754
Size: 793 6768
EcoO109I rG'GnC_Cy
Cuts at: 2195 2196 2848 3106 3183 3353 3372 3654 3999
Size: 1 652 258 77 170 19 282 345
Cuts at: 3999 4096 4249 4348 4486 4487 4617 4750 4804
Size: 97 153 99 138 1 130 133 54
Cuts at: 4804 2195
Size: 4952
Fragments arranged by size:
4952 652 345 282 258 170 153 138
133 130 99 97 77 54 19 1
1
EcoRI G'AATT_C
Cuts at: 4870 5248 4870
Size: 378 7183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 7561
FspI TGC'GCA
Cuts at: 6067 6067
Size: 7561
HaeII r_GCGC'y
Cuts at: 1678 2575 2717 2906 3316 3764 4742 6708 1678
Size: 897 142 189 410 448 978 1966 2531
Fragments arranged by size:
2531 1966 978 897 448 410 189 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 2903 2935 3316
Size: 32 135 32 970 32 329 32 381
Cuts at: 3316 3348 6202 6234 6276 6308 1373
Size: 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 970 381 329 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 4905 5151 669
Size: 988 281 1062 1905 246 3079
Fragments arranged by size:
3079 1905 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7561
HpaI GTT'AAC
Cuts at: 5151 5151
Size: 7561
KasI G'GCGC_C
Cuts at: 2713 4738 2713
Size: 2025 5536
KpnI G_GTAC'C
Cuts at: 4886 4886
Size: 7561
MfeI C'AATT_G
Cuts at: 5160 5160
Size: 7561
MluI A'CGCG_T
Cuts at: 7437 7437
Size: 7561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3597 4782 4800 6675 6859 1305
Size: 2292 1185 18 1875 184 2007
Fragments arranged by size:
2292 2007 1875 1185 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3819 4643 4733 10
Size: 54 1425 343 1987 824 90 2838
Fragments arranged by size:
2838 1987 1425 824 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2708 2960 4265 4658 5519 5878
Size: 648 843 699 252 1305 393 861 359
Cuts at: 5878 6037 518
Size: 159 2042
Fragments arranged by size:
2042 1305 861 843 699 648 393 359
252 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2322 2680 2746 2820 3129 3430 3994
Size: 469 247 358 66 74 309 301 564
Cuts at: 3994 4705 4854 4977 5654 6802 7047 1606
Size: 711 149 123 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 564 469 358 309
301 247 245 149 123 74 66
NaeI GCC'GGC
Cuts at: 3311 3835 4074 4428 3311
Size: 524 239 354 6444
Fragments arranged by size:
6444 524 354 239
NarI GG'CG_CC
Cuts at: 2714 4739 2714
Size: 2025 5536
NcoI C'CATG_G
Cuts at: 513 1065 2004 2955 3282 4524 513
Size: 552 939 951 327 1242 3550
Fragments arranged by size:
3550 1242 951 939 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7561
NgoMIV G'CCGG_C
Cuts at: 3309 3833 4072 4426 3309
Size: 524 239 354 6444
Fragments arranged by size:
6444 524 354 239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7561
NotI GC'GGCC_GC
Cuts at: 5008 5008
Size: 7561
NspI r_CATG'y
Cuts at: 1132 1229 3550 4919 6464 1132
Size: 97 2321 1369 1545 2229
Fragments arranged by size:
2321 2229 1545 1369 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7561
PciI A'CATG_T
Cuts at: 6460 6460
Size: 7561
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3166 1108
Size: 2058 5503
PfoI T'CCnGG_A
Cuts at: 2148 3012 3233 3267 4188 4806 2148
Size: 864 221 34 921 618 4903
Fragments arranged by size:
4903 921 864 618 221 34
PmeI GTTT'AAAC
Cuts at: 4865 4865
Size: 7561
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2446 5630 5662 7143 7175 2414
Size: 32 3184 32 1481 32 2800
Fragments arranged by size:
3184 2800 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2848 3654 4750 4804 2848
Size: 806 1096 54 5605
Fragments arranged by size:
5605 1096 806 54
PshAI GACnn'nnGTC
Cuts at: 1613 3384 1613
Size: 1771 5790
PsiI TTA'TAA
Cuts at: 5131 5131
Size: 7561
PspOMI G'GGCC_C
Cuts at: 2195 3034 3183 3352 4486 2195
Size: 839 149 169 1134 5270
Fragments arranged by size:
5270 1134 839 169 149
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 7529
PstI C_TGCA'G
Cuts at: 830 2358 2973 3903 4332 4378 4485 4704 4710
Size: 1528 615 930 429 46 107 219 6
Cuts at: 4710 4913 830
Size: 203 3478
Fragments arranged by size:
3478 1528 930 615 429 219 203 107
46 6
PvuI CG_AT'CG
Cuts at: 2001 4659 2001
Size: 2658 4903
PvuII CAG'CTG
Cuts at: 2680 2746 2820 3994 4705 4854 2680
Size: 66 74 1174 711 149 5387
Fragments arranged by size:
5387 1174 711 149 74 66
SacI G_AGCT'C
Cuts at: 2432 3863 2432
Size: 1431 6130
SacII CC_GC'GG
Cuts at: 2323 2323
Size: 7561
SalI G'TCGA_C
Cuts at: 1936 2998 4903 1936
Size: 1062 1905 4594
Fragments arranged by size:
4594 1905 1062
SanDI GG'GwC_CC
Cuts at: 3654 3654
Size: 7561
SbfI CC_TGCA'GG
Cuts at: 4485 4913 4485
Size: 428 7133
ScaI AGT'ACT
Cuts at: 5809 7521 5809
Size: 1712 5849
SexAI A'CCwGG_T
Cuts at: 3528 3528
Size: 7561
SfcI C'TryA_G
Cuts at: 826 1045 2354 2629 2969 3367 3899 4328 4374
Size: 219 1309 275 340 398 532 429 46
Cuts at: 4374 4481 4700 4706 4786 4909 6044 6725 6916
Size: 107 219 6 80 123 1135 681 191
Cuts at: 6916 826
Size: 1471
Fragments arranged by size:
1471 1309 1135 681 532 429 398 340
275 219 219 191 123 107 80 46
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 4082 4283 4082
Size: 201 7360
SfoI GGC'GCC
Cuts at: 2715 4740 2715
Size: 2025 5536
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7561
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7561
SmaI CCC'GGG
Cuts at: 1872 2201 3451 4888 1872
Size: 329 1250 1437 4545
Fragments arranged by size:
4545 1437 1250 329
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5665 6566 6828 820
Size: 197 926 3722 901 262 1553
Fragments arranged by size:
3722 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5352 493
Size: 4859 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7561
SphI G_CATG'C
Cuts at: 1132 4919 1132
Size: 3787 3774
SspI AAT'ATT
Cuts at: 5 52 5485 5
Size: 47 5433 2081
Fragments arranged by size:
5433 2081 47
StuI AGG'CCT
Cuts at: 1517 2781 1517
Size: 1264 6297
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2487 2586 2955 3282 4200
Size: 552 939 60 423 99 369 327 918
Cuts at: 4200 4308 4351 4524 4548 4800 5000 513
Size: 108 43 173 24 252 200 3074
Fragments arranged by size:
3074 939 918 552 423 369 327 252
200 173 108 99 60 43 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2178 2315 2410 3752 4405 5588 5605
Size: 1255 598 137 95 1342 653 1183 17
Cuts at: 5605 5758 5943 6388 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1342 1255 1183 653 598 445 185
153 137 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5807 7519 96
Size: 274 80 33 51 1443 3830 1712 138
Fragments arranged by size:
3830 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7521 7553 7521
Size: 32 7529
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7561
XbaI T'CTAG_A
Cuts at: 4897 4897
Size: 7561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 3431 3613 4460 2413
Size: 1018 182 847 5514
Fragments arranged by size:
5514 1018 847 182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7561
XmaI C'CCGG_G
Cuts at: 1870 2199 3449 4886 1870
Size: 329 1250 1437 4545
Fragments arranged by size:
4545 1437 1250 329
XmnI GAAnn'nnTTC
Cuts at: 5690 5690
Size: 7561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2254 3202 3940 5369
Size: 53 83 186 936 720 948 738 1429
Cuts at: 5369 276
Size: 2468
Fragments arranged by size:
2468 1429 948 936 738 720 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI RsrII SapI SrfI SwaI