Restriction map for
pFN21AB4171
(Circular) (Six-base) MAPSORT of: pFN21AB4171.seq Check: 643 from: 1 to: 8158
pFN21AB4171 8158 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5968 278
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3559 5501 1937
Size: 1622 1942 4594
Fragments arranged by size:
4594 1942 1622
Acc65I G'GTAC_C
Cuts at: 4213 5479 4213
Size: 1266 6892
AclI AA'CG_TT
Cuts at: 1563 6285 6658 1563
Size: 4722 373 3063
Fragments arranged by size:
4722 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4075 4619 4931 6221 7605 7873 1632
Size: 2443 544 312 1290 1384 268 1917
Fragments arranged by size:
2443 1917 1384 1290 544 312 268
AfeI AGC'GCT
Cuts at: 2906 2906
Size: 8158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7961
AflIII A'CryG_T
Cuts at: 1203 2203 2643 3834 7057 8034 1203
Size: 1000 440 1191 3223 977 1327
Fragments arranged by size:
3223 1327 1191 1000 977 440
AgeI A'CCGG_T
Cuts at: 6989 6989
Size: 8158
AhdI GACnn_n'nnGTC
Cuts at: 3106 6887 3106
Size: 3781 4377
AleI CACnn'nnGTG
Cuts at: 1166 5441 1166
Size: 4275 3883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4740 5375 5414 1184
Size: 3556 635 39 3928
Fragments arranged by size:
3928 3556 635 39
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 3947 3979 2510
Size: 32 1405 32 6689
Fragments arranged by size:
6689 1405 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2507 4876 5024 5069 5901 7473 2507
Size: 2369 148 45 832 1572 3192
Fragments arranged by size:
3192 2369 1572 832 148 45
ApaI G_GGCC'C
Cuts at: 5101 5120 5101
Size: 19 8139
ApaLI G'TGCA_C
Cuts at: 6215 7371 6215
Size: 1156 7002
ApoI r'AATT_y
Cuts at: 1493 2300 5467 5693 5845 7954 1493
Size: 807 3167 226 152 2109 1697
Fragments arranged by size:
3167 2109 1697 807 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6712 160
Size: 872 5680 1606
Fragments arranged by size:
5680 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
AvaI C'yCGr_G
Cuts at: 1870 1943 2548 3344 4152 4371 4419 5419 5483
Size: 73 605 796 808 219 48 1000 64
Cuts at: 5483 1870
Size: 4545
Fragments arranged by size:
4545 1000 808 796 605 219 73 64
48
AvrII C'CTAG_G
Cuts at: 2705 4060 2705
Size: 1355 6803
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4841 4874 4841
Size: 33 8125
BamHI G'GATC_C
Cuts at: 2649 2727 5488 5855 2649
Size: 78 2761 367 4952
Fragments arranged by size:
4952 2761 367 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3128 3628 3715 4213 5301
Size: 325 217 627 1341 500 87 498 1088
Cuts at: 5301 5390 5479 6934 618
Size: 89 89 1455 1842
Fragments arranged by size:
1842 1455 1341 1088 627 500 498 325
217 89 89 87
BanII G_rGCy'C
Cuts at: 1391 2454 3042 3340 4410 4933 5101 5120 5185
Size: 1063 588 298 1070 523 168 19 65
Cuts at: 5185 5426 5477 1391
Size: 241 51 4072
Fragments arranged by size:
4072 1070 1063 588 523 298 241 168
65 51 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2775 3021 4010 4035 4637 928
Size: 971 876 246 989 25 602 4449
Fragments arranged by size:
4449 989 971 876 602 246 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3995 4029 6349 6383 1903
Size: 34 2058 34 2320 34 3678
Fragments arranged by size:
3678 2320 2058 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2550 6050 7260 2550
Size: 3500 1210 3448
Fragments arranged by size:
3500 3448 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4133 4691 5336
Size: 107 122 71 631 605 2461 558 645
Cuts at: 5336 6769 136
Size: 1433 1525
Fragments arranged by size:
2461 1525 1433 645 631 605 558 122
107 71
BglII A'GATC_T
Cuts at: 3384 8153 3384
Size: 4769 3389
BlpI GC'TnA_GC
Cuts at: 4713 5575 4713
Size: 862 7296
Bme1580I G_kGCm'C
Cuts at: 1790 2441 5101 5120 5304 6219 7375 1790
Size: 651 2660 19 184 915 1156 2573
Fragments arranged by size:
2660 2573 1156 915 651 184 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 2626 3110
Size: 416 53 83 399 24 648 561 484
Cuts at: 3110 5358 5889 6847 442
Size: 2248 531 958 1753
Fragments arranged by size:
2248 1753 958 648 561 531 484 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3766 4210 3766
Size: 444 7714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3363 3944 4061 4841 4874 5046 5088 5199
Size: 2124 581 117 780 33 172 42 111
Cuts at: 5199 5388 6818 1239
Size: 189 1430 2579
Fragments arranged by size:
2579 2124 1430 780 581 189 172 117
111 42 33
Bpu10I CC'TnA_GC
Cuts at: 3729 4338 3729
Size: 609 7549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6283 7148 7446 6283
Size: 865 298 6995
Fragments arranged by size:
6995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4533 4766 6821 882
Size: 617 3034 233 2055 2219
Fragments arranged by size:
3034 2219 2055 617 233
BsaAI yAC'GTr
Cuts at: 493 1204 2646 5949 493
Size: 711 1442 3303 2702
Fragments arranged by size:
3303 2702 1442 711
BsaBI GATnn'nnATC
Cuts at: 2862 8152 2862
Size: 5290 2868
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5965 6347 6982 275
Size: 53 83 186 936 4432 382 635 1451
Fragments arranged by size:
4432 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6591 6989 7263 7410 7821 6591
Size: 398 274 147 411 6928
Fragments arranged by size:
6928 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 2786 2816 1178
Size: 30 165 30 1107 30 246 30 6520
Fragments arranged by size:
6520 1107 246 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 4202 4364 4457 4502 4829 5253 1186
Size: 1834 1182 162 93 45 327 424 4091
Fragments arranged by size:
4091 1834 1182 424 327 162 93 45
BseYI C'CCAG_C
Cuts at: 2653 3284 3683 5001 5027 5187 7361 7857 2653
Size: 631 399 1318 26 160 2174 496 2954
Fragments arranged by size:
2954 2174 1318 631 496 399 160 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2597 1163
Size: 573 684 177 6724
Fragments arranged by size:
6724 684 573 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5608 6369 7397 1540
Size: 275 186 3607 761 1028 2301
Fragments arranged by size:
3607 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4121 6219 6304 7375 4121
Size: 2098 85 1071 4904
Fragments arranged by size:
4904 2098 1071 85
BsmI GAATG_Cn'
Cuts at: 5669 5762 5669
Size: 93 8065
BsmBI CGTCTCn'nnnn_
Cuts at: 3164 3716 3164
Size: 552 7606
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2441 2454 3042 3340 4121 4410 4933
Size: 399 651 13 588 298 781 289 523
Cuts at: 4933 5101 5120 5185 5304 5426 5477 6219 6304
Size: 168 19 65 119 122 51 742 85
Cuts at: 6304 7375 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 781 742 651 588 523 399
298 289 168 122 119 85 65 51
19 13
BspEI T'CCGG_A
Cuts at: 7821 7821
Size: 8158
BspHI T'CATG_A
Cuts at: 2586 3495 6047 7777 2586
Size: 909 2552 1730 2967
Fragments arranged by size:
2967 2552 1730 909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 3246 5513 844
Size: 1749 653 2267 3489
Fragments arranged by size:
3489 2267 1749 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3331 4342 4356 4645 6045 1394
Size: 429 398 1110 1011 14 289 1400 3507
Fragments arranged by size:
3507 1400 1110 1011 429 398 289 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 2218 2368 4311 6653 6827 65
Size: 1183 970 150 1943 2342 174 1396
Fragments arranged by size:
2342 1943 1396 1183 970 174 150
BsrFI r'CCGG_y
Cuts at: 1700 1810 6802 6989 1700
Size: 110 4992 187 2869
Fragments arranged by size:
4992 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2164 3837 3939 96
Size: 2068 1673 102 4315
Fragments arranged by size:
4315 2068 1673 102
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8158
BssSI C'ACGA_G
Cuts at: 3888 6218 7230 3888
Size: 2330 1012 4816
Fragments arranged by size:
4816 2330 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3881 794
Size: 3087 5071
BstBI TT'CG_AA
Cuts at: 4243 6962 4243
Size: 2719 5439
BstEII G'GTnAC_C
Cuts at: 1184 5434 1184
Size: 4250 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 4225 4225
Size: 8158
BstYI r'GATC_y
Cuts at: 1972 2649 2727 3384 5488 5855 6240 6257 7709
Size: 677 78 657 2104 367 385 17 1452
Cuts at: 7709 7795 7807 8153 1972
Size: 86 12 346 1977
Fragments arranged by size:
2104 1977 1452 677 657 385 367 346
86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3651 3651
Size: 8158
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 4218 5044 5230 513
Size: 552 939 51 2163 826 186 3441
Fragments arranged by size:
3441 2163 939 826 552 186 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7419
Fragments arranged by size:
7419 683 56
BtsI GCAGTG_nn'
Cuts at: 4312 4710 5098 5292 5375 5674 6468 6488 4312
Size: 398 388 194 83 299 794 20 5982
Fragments arranged by size:
5982 794 398 388 299 194 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8123
DraI TTT'AAA
Cuts at: 5462 5462
Size: 8158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4547 7165 809
Size: 507 3231 2618 1802
Fragments arranged by size:
3231 2618 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2992 4692 4878
Size: 54 1425 50 275 18 1162 1700 186
Cuts at: 4878 5605 6494 8123 8
Size: 727 889 1629 43
Fragments arranged by size:
1700 1629 1425 1162 889 727 275 186
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5605 1537
Size: 275 3793 4090
Fragments arranged by size:
4090 3793 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2559 2585 2891 2997 3057 4203 4523 6088
Size: 1206 26 306 106 60 1146 320 1565
Cuts at: 6088 1353
Size: 3423
Fragments arranged by size:
3423 1565 1206 1146 320 306 106 60
26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3570 4295 6745 7119 7265 387
Size: 1064 138 1981 725 2450 374 146 1280
Fragments arranged by size:
2450 1981 1280 1064 725 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3363 3944 4061 4075 4619 4841 4874
Size: 393 1731 581 117 14 544 222 33
Cuts at: 4874 4931 5046 5088 5199 5388 6221 6818 7605
Size: 57 115 42 111 189 833 597 787
Cuts at: 7605 7873 1239
Size: 268 1524
Fragments arranged by size:
1731 1524 833 787 597 581 544 393
268 222 189 117 115 111 57 42
33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3242 3783 3023
Size: 219 541 7398
Fragments arranged by size:
7398 541 219
EcoO109I rG'GnC_Cy
Cuts at: 2960 3575 4064 5097 5098 5117 2960
Size: 615 489 1033 1 19 6001
Fragments arranged by size:
6001 1033 615 489 19 1
EcoRI G'AATT_C
Cuts at: 5467 5845 5467
Size: 378 7780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3461 3461
Size: 8158
FspI TGC'GCA
Cuts at: 3827 6664 3827
Size: 2837 5321
HaeII r_GCGC'y
Cuts at: 1678 2908 7305 1678
Size: 1230 4397 2531
Fragments arranged by size:
4397 2531 1230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 3092 3124 3214
Size: 32 135 32 1454 32 34 32 90
Cuts at: 3214 3246 3602 3634 4505 4537 4915 4947 6799
Size: 32 356 32 871 32 378 32 1852
Cuts at: 6799 6831 6873 6905 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1852 1454 871 378 356 135 90
42 34 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 3560 5148 5502 5748 669
Size: 988 281 1150 472 1588 354 246 3079
Fragments arranged by size:
3079 1588 1150 988 472 354 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8158
HpaI GTT'AAC
Cuts at: 5748 5748
Size: 8158
KpnI G_GTAC'C
Cuts at: 4217 5483 4217
Size: 1266 6892
MfeI C'AATT_G
Cuts at: 5757 5757
Size: 8158
MluI A'CGCG_T
Cuts at: 8034 8034
Size: 8158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2714 4770 4972 7272 7456 1305
Size: 1409 2056 202 2300 184 2007
Fragments arranged by size:
2300 2056 2007 1409 202 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2994 10
Size: 54 1425 343 1162 5174
Fragments arranged by size:
5174 1425 1162 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3597 3833 5441 6116 6475 6634
Size: 648 843 1588 236 1608 675 359 159
Cuts at: 6634 518
Size: 2042
Fragments arranged by size:
2042 1608 1588 843 675 648 359 236
159
MspA1I CmG'CkG
Cuts at: 1606 2057 2291 4676 4909 5177 5298 5342 5574
Size: 451 234 2385 233 268 121 44 232
Cuts at: 5574 6251 7399 7644 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2385 2120 1148 677 451 268 245 234
233 232 121 44
NcoI C'CATG_G
Cuts at: 513 1065 2004 5044 5230 513
Size: 552 939 3040 186 3441
Fragments arranged by size:
3441 3040 939 552 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8158
NotI GC'GGCC_GC
Cuts at: 5605 5605
Size: 8158
NspI r_CATG'y
Cuts at: 1132 1229 2207 3811 3838 3974 5516 7061 1132
Size: 97 978 1604 27 136 1542 1545 2229
Fragments arranged by size:
2229 1604 1545 1542 978 136 97 27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8158
PciI A'CATG_T
Cuts at: 2203 3834 7057 2203
Size: 1631 3223 3304
Fragments arranged by size:
3304 3223 1631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3604 5050 1108
Size: 2496 1446 4216
Fragments arranged by size:
4216 2496 1446
PfoI T'CCnGG_A
Cuts at: 2730 3921 5104 2730
Size: 1191 1183 5784
Fragments arranged by size:
5784 1191 1183
PmeI GTTT'AAAC
Cuts at: 5462 5462
Size: 8158
PmlI CAC'GTG
Cuts at: 2646 2646
Size: 8158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 6227 6259 7740 7772 2510
Size: 32 3685 32 1481 32 2896
Fragments arranged by size:
3685 2896 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2960 2960
Size: 8158
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8158
PsiI TTA'TAA
Cuts at: 5728 5728
Size: 8158
PspOMI G'GGCC_C
Cuts at: 5097 5116 5097
Size: 19 8139
PstI C_TGCA'G
Cuts at: 830 3639 3877 5155 5510 830
Size: 2809 238 1278 355 3478
Fragments arranged by size:
3478 2809 1278 355 238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8158
PvuII CAG'CTG
Cuts at: 2291 4909 5177 5342 2291
Size: 2618 268 165 5107
Fragments arranged by size:
5107 2618 268 165
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 8158
SalI G'TCGA_C
Cuts at: 1936 3558 5500 1936
Size: 1622 1942 4594
Fragments arranged by size:
4594 1942 1622
SbfI CC_TGCA'GG
Cuts at: 5510 5510
Size: 8158
ScaI AGT'ACT
Cuts at: 2045 6406 8118 2045
Size: 4361 1712 2085
Fragments arranged by size:
4361 2085 1712
SexAI A'CCwGG_T
Cuts at: 3540 3711 3540
Size: 171 7987
SfcI C'TryA_G
Cuts at: 826 1045 3635 3873 4437 5070 5151 5506 6641
Size: 219 2590 238 564 633 81 355 1135
Cuts at: 6641 7322 7513 826
Size: 681 191 1471
Fragments arranged by size:
2590 1471 1135 681 633 564 355 238
219 191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8158
SmaI CCC'GGG
Cuts at: 1872 2550 5485 1872
Size: 678 2935 4545
Fragments arranged by size:
4545 2935 678
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6262 7163 7425 820
Size: 197 926 4319 901 262 1553
Fragments arranged by size:
4319 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5949 493
Size: 5456 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8158
SphI G_CATG'C
Cuts at: 1132 5516 1132
Size: 4384 3774
SspI AAT'ATT
Cuts at: 5 52 6082 5
Size: 47 6030 2081
Fragments arranged by size:
6030 2081 47
StuI AGG'CCT
Cuts at: 1517 4055 1517
Size: 2538 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 2705 3678 4060 4224 4410
Size: 552 939 390 311 973 382 164 186
Cuts at: 4410 5044 5230 5597 513
Size: 634 186 367 3074
Fragments arranged by size:
3074 973 939 634 552 390 382 367
311 186 186 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 3152 4283 5319 6185 6202 6355
Size: 1255 569 1003 1131 1036 866 17 153
Cuts at: 6355 6540 6985 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1131 1036 1003 866 569 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2043 2164 3837
Size: 274 80 33 51 1443 66 121 1673
Cuts at: 3837 3939 6404 8116 96
Size: 102 2465 1712 138
Fragments arranged by size:
2465 1712 1673 1443 274 138 121 102
80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5025 5057 8118 8150 5025
Size: 32 3061 32 5033
Fragments arranged by size:
5033 3061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8158
XbaI T'CTAG_A
Cuts at: 2557 5494 2557
Size: 2937 5221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3139 4225 3139
Size: 1086 7072
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8158
XmaI C'CCGG_G
Cuts at: 1870 2548 5483 1870
Size: 678 2935 4545
Fragments arranged by size:
4545 2935 678
XmnI GAAnn'nnTTC
Cuts at: 6287 6287
Size: 8158
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5966 276
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacI SanDI
SapI SfiI SfoI SrfI SwaI