Restriction map for
pFN21AB4166
(Circular) (Six-base) MAPSORT of: pFN21AB4166.seq Check: 7141 from: 1 to: 5887
pFN21AB4166 5887 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2028 3230 1937
Size: 91 1202 4594
Fragments arranged by size:
4594 1202 91
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3950 5334 5602 1632
Size: 2318 1384 268 1917
Fragments arranged by size:
2318 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2334 4786 5763 1203
Size: 1131 2452 977 1327
Fragments arranged by size:
2452 1327 1131 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 1184
Size: 1597 4290
AlwNI CAG_nnn'CTG
Cuts at: 2993 3630 5202 2993
Size: 637 1572 3678
Fragments arranged by size:
3678 1572 637
ApaI G_GGCC'C
Cuts at: 3115 3165 3115
Size: 50 5837
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 3196 3422 3574 5683 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3136 3212 1870
Size: 73 67 1126 76 4545
Fragments arranged by size:
4545 1126 76 73 67
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2429 3020 3050 3080 3208
Size: 325 217 627 642 591 30 30 128
Cuts at: 3208 4663 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 642 627 591 325 217 128
30 30
BanII G_rGCy'C
Cuts at: 1391 3115 3165 3206 1391
Size: 1724 50 41 4072
Fragments arranged by size:
4072 1724 50 41
BbeI G_GCGC'C
Cuts at: 2433 2433
Size: 5887
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BbvCI CC'TCA_GC
Cuts at: 2037 2910 2037
Size: 873 5014
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 3779 4989 2936
Size: 843 1210 3834
Fragments arranged by size:
3834 1210 843
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2541 2886 5882 2541
Size: 345 2996 2546
Fragments arranged by size:
2996 2546 345
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2765 3053 3083 3115 3165 3948 5104 1790
Size: 975 288 30 32 50 783 1156 2573
Fragments arranged by size:
2573 1156 975 783 288 50 32 30
BmgBI CAC'GTC
Cuts at: 1318 2140 1318
Size: 822 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2536 3618 4576
Size: 416 53 83 399 24 1119 1082 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1119 1082 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2961 1056
Size: 1905 3982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2394 2735 4547 1239
Size: 1155 341 1812 2579
Fragments arranged by size:
2579 1812 1155 341
Bpu10I CC'TnA_GC
Cuts at: 2037 2910 2037
Size: 873 5014
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 4012 4877 5175 2840
Size: 1172 865 298 3552
Fragments arranged by size:
3552 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 3184 4550 882
Size: 617 561 1124 1366 2219
Fragments arranged by size:
2219 1366 1124 617 561
BsaAI yAC'GTr
Cuts at: 493 1204 2284 3678 493
Size: 711 1080 1394 2702
Fragments arranged by size:
2702 1394 1080 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 2430 3694 4076
Size: 53 83 186 936 756 141 1264 382
Cuts at: 4076 4711 275
Size: 635 1451
Fragments arranged by size:
1451 1264 936 756 635 382 186 141
83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 1186
Size: 1657 4230
BseYI C'CCAG_C
Cuts at: 2262 2892 3146 5090 5586 2262
Size: 630 254 1944 496 2563
Fragments arranged by size:
2563 1944 630 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2660 2951 1163
Size: 573 924 291 4099
Fragments arranged by size:
4099 924 573 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2765 3053 3083 3115 3165 3206 3948
Size: 399 975 288 30 32 50 41 742
Cuts at: 3948 4033 5104 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 975 742 399 288 85 50
41 32 30
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 2682 3242 844
Size: 1381 457 560 3489
Fragments arranged by size:
3489 1381 560 457
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 3063 3093 3774 1394
Size: 429 1210 30 30 681 3507
Fragments arranged by size:
3507 1210 681 429 30 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 2169 2703 2896 4531 4718 1700
Size: 110 265 94 534 193 1635 187 2869
Fragments arranged by size:
2869 1635 534 265 193 187 110 94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 794
Size: 2010 3877
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2591 3172 2591
Size: 581 5306
BstYI r'GATC_y
Cuts at: 1972 2541 2886 3217 3584 3969 3986 5438 5524
Size: 569 345 331 367 385 17 1452 86
Cuts at: 5524 5536 5882 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 569 385 367 346 345 331
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2346 2808 3171 513
Size: 552 1281 462 363 3229
Fragments arranged by size:
3229 1281 552 462 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2765 2805 3403 4197 4217 2765
Size: 40 598 794 20 4435
Fragments arranged by size:
4435 794 598 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2148 2650 2148
Size: 502 5385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 2898 3334
Size: 54 1425 50 275 18 466 602 436
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 602 466 436 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 3817 1353
Size: 884 1580 3423
Fragments arranged by size:
3423 1580 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2879 4474 4848 4994 387
Size: 1064 138 1290 1595 374 146 1280
Fragments arranged by size:
1595 1290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2735 3950 4547 5334 5602 1239
Size: 393 762 341 1215 597 787 268 1524
Fragments arranged by size:
1524 1215 787 762 597 393 341 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2036
Size: 5887
EcoO109I rG'GnC_Cy
Cuts at: 2479 3111 2479
Size: 632 5255
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2433 3159 5034 1678
Size: 755 726 1875 2531
Fragments arranged by size:
2531 1875 755 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2545 2577 4528 4560 4602
Size: 32 135 32 973 32 1951 32 42
Cuts at: 4602 4634 1373
Size: 32 2626
Fragments arranged by size:
2626 1951 973 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KasI G'GCGC_C
Cuts at: 2429 2429
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2298 10
Size: 54 1425 343 466 3599
Fragments arranged by size:
3599 1425 466 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2576 3845 4204 4363 518
Size: 648 1035 375 1269 359 159 2042
Fragments arranged by size:
2042 1269 1035 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2356 3303 3980 5128 5373 1606
Size: 750 947 677 1148 245 2120
Fragments arranged by size:
2120 1148 947 750 677 245
NaeI GCC'GGC
Cuts at: 2705 2898 2705
Size: 193 5694
NarI GG'CG_CC
Cuts at: 2430 2430
Size: 5887
NcoI C'CATG_G
Cuts at: 513 1065 2346 3171 513
Size: 552 1281 825 3229
Fragments arranged by size:
3229 1281 825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NgoMIV G'CCGG_C
Cuts at: 2703 2896 2703
Size: 193 5694
NheI G'CTAG_C
Cuts at: 1052 2957 1052
Size: 1905 3982
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2251 2338 2950 3245 4790 1132
Size: 97 1022 87 612 295 1545 2229
Fragments arranged by size:
2229 1545 1022 612 295 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 2334 4786 2334
Size: 2452 3435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PfoI T'CCnGG_A
Cuts at: 2100 2371 2100
Size: 271 5616
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PmlI CAC'GTG
Cuts at: 2284 2284
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PspOMI G'GGCC_C
Cuts at: 3111 3161 3111
Size: 50 5837
PstI C_TGCA'G
Cuts at: 830 2760 2800 3239 830
Size: 1930 40 439 3478
Fragments arranged by size:
3478 1930 439 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2356 2356
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 2924 4135 5847 2924
Size: 1211 1712 2964
Fragments arranged by size:
2964 1712 1211
SfcI C'TryA_G
Cuts at: 826 1045 2756 2796 3235 4370 5051 5242 826
Size: 219 1711 40 439 1135 681 191 1471
Fragments arranged by size:
1711 1471 1135 681 439 219 191 40
SfoI GGC'GCC
Cuts at: 2431 2431
Size: 5887
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2855 3991 4892 5154 820
Size: 197 926 912 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 912 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2915 1517
Size: 1398 4489
StyI C'CwwG_G
Cuts at: 513 1065 2160 2346 3171 3326 513
Size: 552 1095 186 825 155 3074
Fragments arranged by size:
3074 1095 825 552 186 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2276 3914 3931 4084 4269 4714 325
Size: 1255 696 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 696 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 4133 5845
Size: 274 80 33 51 1443 945 1211 1712
Cuts at: 5845 96
Size: 138
Fragments arranged by size:
1712 1443 1211 945 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2353
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2367 4016 2367
Size: 1649 4238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI