Restriction map for
pFN21AB4165
(Circular) (Six-base) MAPSORT of: pFN21AB4165.seq Check: 6987 from: 1 to: 7183
pFN21AB4165 7183 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2848 2856 2866 3157 3453 2848
Size: 8 10 291 296 6578
Fragments arranged by size:
6578 296 291 10 8
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4125 4993 278
Size: 53 83 186 936 2589 868 2468
Fragments arranged by size:
2589 2468 936 868 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4526 1937
Size: 2589 4594
Acc65I G'GTAC_C
Cuts at: 4504 4504
Size: 7183
AclI AA'CG_TT
Cuts at: 1563 5310 5683 1563
Size: 3747 373 3063
Fragments arranged by size:
3747 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2903 5246 6630 6898 1632
Size: 719 552 2343 1384 268 1917
Fragments arranged by size:
2343 1917 1384 719 552 268
AfeI AGC'GCT
Cuts at: 3724 3724
Size: 7183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6986
AflIII A'CryG_T
Cuts at: 1203 3516 6082 7059 1203
Size: 2313 2566 977 1327
Fragments arranged by size:
2566 2313 1327 977
AgeI A'CCGG_T
Cuts at: 6014 6014
Size: 7183
AhdI GACnn_n'nnGTC
Cuts at: 2554 5912 2554
Size: 3358 3825
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2521 2609 4091 1184
Size: 1337 88 1482 4276
Fragments arranged by size:
4276 1482 1337 88
AlwNI CAG_nnn'CTG
Cuts at: 2848 2881 2902 2989 3007 3265 3538 4926 6498
Size: 33 21 87 18 258 273 1388 1572
Cuts at: 6498 2848
Size: 3533
Fragments arranged by size:
3533 1572 1388 273 258 87 33 21
18
ApaI G_GGCC'C
Cuts at: 2080 2752 2977 3658 4414 2080
Size: 672 225 681 756 4849
Fragments arranged by size:
4849 756 681 672 225
ApaLI G'TGCA_C
Cuts at: 3074 5240 6396 3074
Size: 2166 1156 3861
Fragments arranged by size:
3861 2166 1156
ApoI r'AATT_y
Cuts at: 1493 4492 4718 4870 6979 1493
Size: 2999 226 152 2109 1697
Fragments arranged by size:
2999 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5737 160
Size: 872 4705 1606
Fragments arranged by size:
4705 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2158 4508 1870
Size: 73 144 71 2350 4545
Fragments arranged by size:
4545 2350 144 73 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2181 2214 3625 3658 4223 4256 2181
Size: 33 1411 33 565 33 5108
Fragments arranged by size:
5108 1411 565 33 33 33
BamHI G'GATC_C
Cuts at: 4451 4513 4880 4451
Size: 62 367 6754
Fragments arranged by size:
6754 367 62
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2709 3596 4504 5959
Size: 325 217 627 603 319 887 908 1455
Cuts at: 5959 618
Size: 1842
Fragments arranged by size:
1842 1455 908 887 627 603 325 319
217
BanII G_rGCy'C
Cuts at: 1391 2080 2088 2263 2304 2752 2912 2977 3055
Size: 689 8 175 41 448 160 65 78
Cuts at: 3055 3159 3321 3658 4414 4502 1391
Size: 104 162 337 756 88 4072
Fragments arranged by size:
4072 756 689 448 337 175 162 160
104 88 78 65 41 8
BbeI G_GCGC'C
Cuts at: 2713 3600 2713
Size: 887 6296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 3481 928
Size: 971 734 848 4630
Fragments arranged by size:
4630 971 848 734
BbvCI CC'TCA_GC
Cuts at: 2643 2819 2643
Size: 176 7007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2717 2751 5374 5408 1903
Size: 34 780 34 2623 34 3678
Fragments arranged by size:
3678 2623 780 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5075 6285 5075
Size: 1210 5973
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 3594 3807
Size: 107 122 71 631 605 457 1465 213
Cuts at: 3807 5794 136
Size: 1987 1525
Fragments arranged by size:
1987 1525 1465 631 605 457 213 122
107 71
BglII A'GATC_T
Cuts at: 3732 7178 3732
Size: 3446 3737
BlpI GC'TnA_GC
Cuts at: 4600 4600
Size: 7183
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2224 2393 2752 2977 3078 3658 3803
Size: 290 144 169 359 225 101 580 145
Cuts at: 3803 4161 4414 5244 6400 1790
Size: 358 253 830 1156 2573
Fragments arranged by size:
2573 1156 830 580 359 358 290 253
225 169 145 144 101
BmgBI CAC'GTC
Cuts at: 1318 2025 3517 1318
Size: 707 1492 4984
Fragments arranged by size:
4984 1492 707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2189 2284
Size: 416 53 83 399 24 767 5 95
Cuts at: 2284 2746 3709 4914 5872 442
Size: 462 963 1205 958 1753
Fragments arranged by size:
1753 1205 963 958 767 462 416 399
95 83 53 24 5
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3952 3952
Size: 7183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2200 2591 2987 3011 3806 5843 1239
Size: 961 391 396 24 795 2037 2579
Fragments arranged by size:
2579 2037 961 795 396 391 24
Bpu10I CC'TnA_GC
Cuts at: 2264 2643 2819 2264
Size: 379 176 6628
Fragments arranged by size:
6628 379 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3143 4265 5308 6173 6471 2645
Size: 498 1122 1043 865 298 3357
Fragments arranged by size:
3357 1122 1043 865 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2723 5846 882
Size: 617 1224 3123 2219
Fragments arranged by size:
3123 2219 1224 617
BsaAI yAC'GTr
Cuts at: 493 1204 4156 4974 493
Size: 711 2952 818 2702
Fragments arranged by size:
2952 2702 818 711
BsaBI GATnn'nnATC
Cuts at: 7177 7177
Size: 7183
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2710 3093 3597 3692
Size: 53 83 186 936 1177 383 504 95
Cuts at: 3692 4122 4391 4990 5372 6007 275
Size: 430 269 599 382 635 1451
Fragments arranged by size:
1451 1177 936 635 599 504 430 383
382 269 186 95 83 53
BsaWI w'CCGG_w
Cuts at: 3096 4458 5616 6014 6288 6435 6846 3096
Size: 1362 1158 398 274 147 411 3433
Fragments arranged by size:
3433 1362 1158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2780 2810 2987
Size: 30 165 30 795 30 552 30 177
Cuts at: 2987 3017 3416 3446 3677 3707 1178
Size: 30 399 30 231 30 4654
Fragments arranged by size:
4654 795 552 399 231 177 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 2708 3008 3050 3254 1186
Size: 1519 3 300 42 204 5115
Fragments arranged by size:
5115 1519 300 204 42 3
BseYI C'CCAG_C
Cuts at: 2113 2119 2239 2383 2538 2559 4312 4424 6386
Size: 6 120 144 155 21 1753 112 1962
Cuts at: 6386 6882 2113
Size: 496 2414
Fragments arranged by size:
2414 1962 1753 496 155 144 120 112
21 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2250 2639 3434 3551 3701 3872 1163
Size: 573 514 389 795 117 150 171 4474
Fragments arranged by size:
4474 795 573 514 389 171 150 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2635 3909 4482 4633 5394
Size: 275 186 132 502 1274 573 151 761
Cuts at: 5394 6422 1540
Size: 1028 2301
Fragments arranged by size:
2301 1274 1028 761 573 502 275 186
151 132
BsiHKAI G_wGCw'C
Cuts at: 2523 2912 3078 3321 3933 5244 5329 6400 2523
Size: 389 166 243 612 1311 85 1071 3306
Fragments arranged by size:
3306 1311 1071 612 389 243 166 85
BsiWI C'GTAC_G
Cuts at: 3582 3582
Size: 7183
BsmI GAATG_Cn'
Cuts at: 4694 4787 4694
Size: 93 7090
BsmBI CGTCTCn'nnnn_
Cuts at: 4259 4259
Size: 7183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2088 2224 2263 2304 2393 2523
Size: 399 290 8 136 39 41 89 130
Cuts at: 2523 2752 2912 2977 3055 3078 3159 3321 3658
Size: 229 160 65 78 23 81 162 337
Cuts at: 3658 3803 3933 4161 4414 4502 5244 5329 6400
Size: 145 130 228 253 88 742 85 1071
Cuts at: 6400 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 337 290 253 229
228 162 160 145 136 130 130 89
88 85 81 78 65 41 39 23
8
BspEI T'CCGG_A
Cuts at: 3096 4458 6846 3096
Size: 1362 2388 3433
Fragments arranged by size:
3433 2388 1362
BspHI T'CATG_A
Cuts at: 5072 6802 5072
Size: 1730 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2308 2424 2489 2848 2856 2866 3157 3453
Size: 1464 116 65 359 8 10 291 296
Cuts at: 3453 3765 4194 4538 844
Size: 312 429 344 3489
Fragments arranged by size:
3489 1464 429 359 344 312 296 291
116 65 10 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2698 2971 3942 5070 1394
Size: 429 875 273 971 1128 3507
Fragments arranged by size:
3507 1128 971 875 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 2733 4125 5678 5852 65
Size: 1183 1485 1392 1553 174 1396
Fragments arranged by size:
1553 1485 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 2634 3121 3599 4378 5827 6014
Size: 110 343 481 487 478 779 1449 187
Cuts at: 6014 1700
Size: 2869
Fragments arranged by size:
2869 1449 779 487 481 478 343 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7183
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7183
BssSI C'ACGA_G
Cuts at: 2726 3077 3320 3354 5243 6255 2726
Size: 351 243 34 1889 1012 3654
Fragments arranged by size:
3654 1889 1012 351 243 34
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 2482 3952 794
Size: 1658 30 1470 4025
Fragments arranged by size:
4025 1658 1470 30
BstBI TT'CG_AA
Cuts at: 5987 5987
Size: 7183
BstEII G'GTnAC_C
Cuts at: 1184 3541 1184
Size: 2357 4826
BstYI r'GATC_y
Cuts at: 1972 3732 3789 4451 4513 4880 5265 5282 6734
Size: 1760 57 662 62 367 385 17 1452
Cuts at: 6734 6820 6832 7178 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1760 1452 662 385 367 346 86
62 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3203 3393 3749 3774 3203
Size: 190 356 25 6612
Fragments arranged by size:
6612 356 190 25
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 2977 4002 4245 513
Size: 552 939 292 681 1025 243 3451
Fragments arranged by size:
3451 1025 939 681 552 292 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6444
Fragments arranged by size:
6444 683 56
BtsI GCAGTG_nn'
Cuts at: 3125 3182 3817 4699 5493 5513 3125
Size: 57 635 882 794 20 4795
Fragments arranged by size:
4795 882 794 635 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7148
DraI TTT'AAA
Cuts at: 4487 4487
Size: 7183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6190 809
Size: 507 4874 1802
Fragments arranged by size:
4874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 3362 3586
Size: 54 1425 50 275 18 300 1232 224
Cuts at: 3586 3889 4479 4630 5519 7148 8
Size: 303 590 151 889 1629 43
Fragments arranged by size:
1629 1425 1232 889 590 303 300 275
224 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 4479 4630 1537
Size: 275 318 2349 151 4090
Fragments arranged by size:
4090 2349 318 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 3006 3674 5113 1353
Size: 1653 668 1439 3423
Fragments arranged by size:
3423 1653 1439 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2594 5770 6144 6290 387
Size: 1064 138 1005 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 1005 374 146 138
EcoICRI GAG'CTC
Cuts at: 2910 3319 2910
Size: 409 6774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2351 2591 2903 2987 3011 3806
Size: 393 568 151 240 312 84 24 795
Cuts at: 3806 5246 5843 6630 6898 1239
Size: 1440 597 787 268 1524
Fragments arranged by size:
1524 1440 795 787 597 568 393 312
268 240 151 84 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2017 3011 4079 4190 2017
Size: 994 1068 111 5010
Fragments arranged by size:
5010 1068 994 111
EcoO109I rG'GnC_Cy
Cuts at: 2056 2077 2413 2501 2815 3199 3389 3535 3655
Size: 21 336 88 314 384 190 146 120
Cuts at: 3655 3745 3754 4316 4344 2056
Size: 90 9 562 28 4895
Fragments arranged by size:
4895 562 384 336 314 190 146 120
90 88 28 21 9
EcoRI G'AATT_C
Cuts at: 4492 4870 4492
Size: 378 6805
FspI TGC'GCA
Cuts at: 2486 3272 5689 2486
Size: 786 2417 3980
Fragments arranged by size:
3980 2417 786
HaeII r_GCGC'y
Cuts at: 1678 2461 2538 2713 3600 3726 6330 1678
Size: 783 77 175 887 126 2604 2531
Fragments arranged by size:
2604 2531 887 783 175 126 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2540 2572 2780
Size: 32 135 32 626 32 310 32 208
Cuts at: 2780 2812 2987 3019 3416 3448 3677 3709 4328
Size: 32 175 32 397 32 229 32 619
Cuts at: 4328 4360 5824 5856 5898 5930 1373
Size: 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 626 619 397 310 229 208
175 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3247 4527 4773 669
Size: 988 281 1309 1280 246 3079
Fragments arranged by size:
3079 1309 1280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7183
HpaI GTT'AAC
Cuts at: 4773 4773
Size: 7183
KasI G'GCGC_C
Cuts at: 2709 3596 2709
Size: 887 6296
KpnI G_GTAC'C
Cuts at: 4508 4508
Size: 7183
MfeI C'AATT_G
Cuts at: 4782 4782
Size: 7183
MluI A'CGCG_T
Cuts at: 7059 7059
Size: 7183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6297 6481 1305
Size: 4992 184 2007
Fragments arranged by size:
4992 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3891 10
Size: 54 1425 343 2059 3302
Fragments arranged by size:
3302 2059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3344 5141 5500 5659 518
Size: 648 2178 1797 359 159 2042
Fragments arranged by size:
2178 2042 1797 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2563 2569 2902 2920 3295 3629 3652 3664
Size: 957 6 333 18 375 334 23 12
Cuts at: 3664 3820 4599 5276 6424 6669 1606
Size: 156 779 677 1148 245 2120
Fragments arranged by size:
2120 1148 957 779 677 375 334 333
245 156 23 18 12 6
NaeI GCC'GGC
Cuts at: 3123 3601 4380 3123
Size: 478 779 5926
Fragments arranged by size:
5926 779 478
NarI GG'CG_CC
Cuts at: 2710 3597 2710
Size: 887 6296
NcoI C'CATG_G
Cuts at: 513 1065 2004 2977 513
Size: 552 939 973 4719
Fragments arranged by size:
4719 973 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7183
NgoMIV G'CCGG_C
Cuts at: 3121 3599 4378 3121
Size: 478 779 5926
Fragments arranged by size:
5926 779 478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7183
NotI GC'GGCC_GC
Cuts at: 4630 4630
Size: 7183
NspI r_CATG'y
Cuts at: 1132 1229 2596 4541 6086 1132
Size: 97 1367 1945 1545 2229
Fragments arranged by size:
2229 1945 1545 1367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7183
PciI A'CATG_T
Cuts at: 6082 6082
Size: 7183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7183
PfoI T'CCnGG_A
Cuts at: 3522 3522
Size: 7183
PmeI GTTT'AAAC
Cuts at: 4487 4487
Size: 7183
PmlI CAC'GTG
Cuts at: 4156 4156
Size: 7183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5284 6765 6797 5252
Size: 32 1481 32 5638
Fragments arranged by size:
5638 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2501 3389 3745 3754 4344 2413
Size: 88 888 356 9 590 5252
Fragments arranged by size:
5252 888 590 356 88 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7183
PsiI TTA'TAA
Cuts at: 4753 4753
Size: 7183
PspOMI G'GGCC_C
Cuts at: 2076 2748 2973 3654 4410 2076
Size: 672 225 681 756 4849
Fragments arranged by size:
4849 756 681 672 225
PstI C_TGCA'G
Cuts at: 830 2319 2568 2853 2907 3195 3294 3663 3986
Size: 1489 249 285 54 288 99 369 323
Cuts at: 3986 4194 4473 4535 830
Size: 208 279 62 3478
Fragments arranged by size:
3478 1489 369 323 288 285 279 249
208 99 62 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7183
PvuII CAG'CTG
Cuts at: 2563 2569 2902 2920 3295 3664 3820 2563
Size: 6 333 18 375 369 156 5926
Fragments arranged by size:
5926 375 369 333 156 18 6
SacI G_AGCT'C
Cuts at: 2912 3321 2912
Size: 409 6774
SalI G'TCGA_C
Cuts at: 1936 4525 1936
Size: 2589 4594
SapI GCTCTTCn'nnn_
Cuts at: 3674 3674
Size: 7183
SbfI CC_TGCA'GG
Cuts at: 2319 2853 4535 2319
Size: 534 1682 4967
Fragments arranged by size:
4967 1682 534
ScaI AGT'ACT
Cuts at: 5431 7143 5431
Size: 1712 5471
SexAI A'CCwGG_T
Cuts at: 4017 4017
Size: 7183
SfcI C'TryA_G
Cuts at: 826 1045 2315 2564 2849 2903 3191 3290 3659
Size: 219 1270 249 285 54 288 99 369
Cuts at: 3659 3982 4190 4469 4531 5666 6347 6538 826
Size: 323 208 279 62 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 369 323 288 285
279 249 219 208 191 99 62 54
SfoI GGC'GCC
Cuts at: 2711 3598 2711
Size: 887 6296
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7183
SmaI CCC'GGG
Cuts at: 1872 2160 4510 1872
Size: 288 2350 4545
Fragments arranged by size:
4545 2350 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 3158 4280 5287 6188 6450
Size: 197 926 717 498 1122 1007 901 262
Cuts at: 6450 820
Size: 1553
Fragments arranged by size:
1553 1122 1007 926 901 717 498 262
197
SnaBI TAC'GTA
Cuts at: 493 4974 493
Size: 4481 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7183
SphI G_CATG'C
Cuts at: 1132 2596 4541 1132
Size: 1464 1945 3774
Fragments arranged by size:
3774 1945 1464
SspI AAT'ATT
Cuts at: 5 52 5107 5
Size: 47 5055 2081
Fragments arranged by size:
5055 2081 47
StuI AGG'CCT
Cuts at: 1517 3041 3503 3993 1517
Size: 1524 462 490 4707
Fragments arranged by size:
4707 1524 490 462
StyI C'CwwG_G
Cuts at: 513 1065 2004 2977 4622 513
Size: 552 939 973 1645 3074
Fragments arranged by size:
3074 1645 973 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2645 3923 5210 5227 5380 5565 6010
Size: 1255 1065 1278 1287 17 153 185 445
Cuts at: 6010 325
Size: 1498
Fragments arranged by size:
1498 1287 1278 1255 1065 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3705 5429 7141
Size: 274 80 33 51 1443 1728 1724 1712
Cuts at: 7141 96
Size: 138
Fragments arranged by size:
1728 1724 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7143 7175 7143
Size: 32 7151
Tth111I GACn'n_nGTC
Cuts at: 1316 3485 1316
Size: 2169 5014
XbaI T'CTAG_A
Cuts at: 4366 4519 4366
Size: 153 7030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3055
Size: 7183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7183
XmaI C'CCGG_G
Cuts at: 1870 2158 4508 1870
Size: 288 2350 4545
Fragments arranged by size:
4545 2350 288
XmnI GAAnn'nnTTC
Cuts at: 5312 5312
Size: 7183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4123 4991 276
Size: 53 83 186 936 2589 868 2468
Fragments arranged by size:
2589 2468 936 868 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SfiI SrfI SwaI