Restriction map for
pFN21AB4158
(Circular) (Six-base) MAPSORT of: pFN21AB4158.seq Check: 7105 from: 1 to: 6181
pFN21AB4158 6181 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2353 2868 3524 1937
Size: 416 515 656 4594
Fragments arranged by size:
4594 656 515 416
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 2827 4308 4681 1563
Size: 1264 1481 373 3063
Fragments arranged by size:
3063 1481 1264 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2435 2754 2825 3213 4244 5628 5896 1632
Size: 803 319 71 388 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 803 388 319 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3389 1184
Size: 2205 3976
AlwNI CAG_nnn'CTG
Cuts at: 3924 5496 3924
Size: 1572 4609
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2103 2425 2882 2941 3490 3716 3868 5977
Size: 610 322 457 59 549 226 152 2109
Cuts at: 5977 1493
Size: 1697
Fragments arranged by size:
2109 1697 610 549 457 322 226 152
59
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 3454 3506 1870
Size: 73 1511 52 4545
Fragments arranged by size:
4545 1511 73 52
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2909 3411 3502 4957 618
Size: 325 217 627 1122 502 91 1455 1842
Fragments arranged by size:
1842 1455 1122 627 502 325 217 91
BanII G_rGCy'C
Cuts at: 1391 2994 3500 1391
Size: 1603 506 4072
Fragments arranged by size:
4072 1603 506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2310 2661 928
Size: 971 411 351 4448
Fragments arranged by size:
4448 971 411 351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2646 4073 5283 2646
Size: 1427 1210 3544
Fragments arranged by size:
3544 1427 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 4792 136
Size: 107 122 71 631 605 1126 1994 1525
Fragments arranged by size:
1994 1525 1126 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 2482 3598 2482
Size: 1116 5065
Bme1580I G_kGCm'C
Cuts at: 1790 4242 5398 1790
Size: 2452 1156 2573
Fragments arranged by size:
2573 2452 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3912 4870 442
Size: 416 53 83 399 24 2495 958 1753
Fragments arranged by size:
2495 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2442 1056
Size: 1386 4795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 2535 2709 4841 1239
Size: 1068 228 174 2132 2579
Fragments arranged by size:
2579 2132 1068 228 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4844 882
Size: 617 3345 2219
Fragments arranged by size:
3345 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2564 3972 493
Size: 711 1360 1408 2702
Fragments arranged by size:
2702 1408 1360 711
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2445 2475 2713 2743 1178
Size: 30 165 30 1042 30 238 30 4616
Fragments arranged by size:
4616 1042 238 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5295
BseYI C'CCAG_C
Cuts at: 5384 5880 5384
Size: 496 5685
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2008 1163
Size: 573 272 5336
Fragments arranged by size:
5336 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4242 4327 5398 4242
Size: 85 1071 5025
Fragments arranged by size:
5025 1071 85
BsmI GAATG_Cn'
Cuts at: 2075 3378 3692 3785 2075
Size: 1303 314 93 4471
Fragments arranged by size:
4471 1303 314 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2994 3500 4242 4327 5398 1391
Size: 399 1204 506 742 85 1071 2174
Fragments arranged by size:
2174 1204 1071 742 506 399 85
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 2233 4070 5800 2233
Size: 1837 1730 2614
Fragments arranged by size:
2614 1837 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 3536 844
Size: 1186 1506 3489
Fragments arranged by size:
3489 1506 1186
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4825 5012 1700
Size: 110 3015 187 2869
Fragments arranged by size:
3015 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3301 96
Size: 3205 2976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 3402 4241 5253 3402
Size: 839 1012 4330
Fragments arranged by size:
4330 1012 839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 3459 1184
Size: 2275 3906
BstXI CCAn_nnnn'nTGG
Cuts at: 2646 2646
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2569 2817 3511 3878 4263 4280 5732 5818
Size: 597 248 694 367 385 17 1452 86
Cuts at: 5818 5830 6176 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 694 597 385 367 346 248
86 17 12
BstZ17I GTA'TAC
Cuts at: 2354 2354
Size: 6181
BtgI C'CryG_G
Cuts at: 513 1065 2004 2813 513
Size: 552 939 809 3881
Fragments arranged by size:
3881 939 809 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3434 487
Size: 683 56 2208 3234
Fragments arranged by size:
3234 2208 683 56
BtsI GCAGTG_nn'
Cuts at: 3373 3697 4491 4511 3373
Size: 324 794 20 5043
Fragments arranged by size:
5043 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3388 5188 809
Size: 507 2072 1800 1802
Fragments arranged by size:
2072 1802 1800 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3628 4517 6146
Size: 54 1425 50 275 18 1798 889 1629
Cuts at: 6146 8
Size: 43
Fragments arranged by size:
1798 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 3390 4111 1353
Size: 891 1146 721 3423
Fragments arranged by size:
3423 1146 891 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2435 2535 2709 2754 2825 3213
Size: 393 675 128 100 174 45 71 388
Cuts at: 3213 4244 4841 5628 5896 1239
Size: 1031 597 787 268 1524
Fragments arranged by size:
1524 1031 787 675 597 393 388 268
174 128 100 71 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2026 2026
Size: 6181
EcoO109I rG'GnC_Cy
Cuts at: 2410 2410
Size: 6181
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
EcoRV GAT'ATC
Cuts at: 2543 2543
Size: 6181
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 5328 1678
Size: 3650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2690 2722 4822
Size: 32 135 32 656 32 430 32 2100
Cuts at: 4822 4854 4896 4928 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2100 656 430 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2825 3036 3525 3771 669
Size: 988 281 887 211 489 246 3079
Fragments arranged by size:
3079 988 887 489 281 246 211
HindIII A'AGCT_T
Cuts at: 748 2150 3195 748
Size: 1402 1045 3734
Fragments arranged by size:
3734 1402 1045
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3057 3780 3057
Size: 723 5458
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4359
Fragments arranged by size:
4359 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2713 4139 4498 4657 518
Size: 648 1225 322 1426 359 159 2042
Fragments arranged by size:
2042 1426 1225 648 359 322 159
MspA1I CmG'CkG
Cuts at: 1606 2459 3597 4274 5422 5667 1606
Size: 853 1138 677 1148 245 2120
Fragments arranged by size:
2120 1148 1138 853 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2813 513
Size: 552 939 809 3881
Fragments arranged by size:
3881 939 809 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 2438 1052
Size: 1386 4795
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 2604 3539 5084 1132
Size: 97 1375 935 1545 2229
Fragments arranged by size:
2229 1545 1375 935 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 2410
Size: 6181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PstI C_TGCA'G
Cuts at: 830 3533 830
Size: 2703 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2459 2459
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SexAI A'CCwGG_T
Cuts at: 2027 2027
Size: 6181
SfcI C'TryA_G
Cuts at: 826 1045 2326 2865 3529 4664 5345 5536 826
Size: 219 1281 539 664 1135 681 191 1471
Fragments arranged by size:
1471 1281 1135 681 664 539 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4285 5186 5448 820
Size: 197 926 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2564 3972 493
Size: 2071 1408 2702
Fragments arranged by size:
2702 2071 1408
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 2221 4105 5
Size: 47 2169 1884 2081
Fragments arranged by size:
2169 2081 1884 47
StuI AGG'CCT
Cuts at: 1517 3174 1517
Size: 1657 4524
StyI C'CwwG_G
Cuts at: 513 1065 2004 2448 2813 3620 513
Size: 552 939 444 365 807 3074
Fragments arranged by size:
3074 939 807 552 444 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2826 4208 4225 4378 4563 5008 325
Size: 1255 1246 1382 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1255 1246 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3301 3354 4427
Size: 274 80 33 51 1443 1324 53 1073
Cuts at: 4427 6139 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1324 1073 274 138 80 53
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2445 2477 2571 2603 2713 2745 3413 3445 6141
Size: 32 94 32 110 32 668 32 2696
Cuts at: 6141 6173 2445
Size: 32 2453
Fragments arranged by size:
2696 2453 668 110 94 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 2365 3517 2365
Size: 1152 5029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2592 2712 3017 2592
Size: 120 305 5756
Fragments arranged by size:
5756 305 120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI