Restriction map for
pFN21AB4151
(Circular) (Six-base) MAPSORT of: pFN21AB4151.seq Check: 4724 from: 1 to: 7879
pFN21AB4151 7879 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3817 3817
Size: 7879
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5689 278
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3771 5222 1937
Size: 1834 1451 4594
Fragments arranged by size:
4594 1834 1451
Acc65I G'GTAC_C
Cuts at: 5200 5200
Size: 7879
AclI AA'CG_TT
Cuts at: 1563 6006 6379 1563
Size: 4443 373 3063
Fragments arranged by size:
4443 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3926 5942 7326 7594 1632
Size: 2294 2016 1384 268 1917
Fragments arranged by size:
2294 2016 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7682
AflIII A'CryG_T
Cuts at: 1203 6778 7755 1203
Size: 5575 977 1327
Fragments arranged by size:
5575 1327 977
AgeI A'CCGG_T
Cuts at: 6710 6710
Size: 7879
AhdI GACnn_n'nnGTC
Cuts at: 6608 6608
Size: 7879
AleI CACnn'nnGTG
Cuts at: 1166 2547 1166
Size: 1381 6498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4106 1184
Size: 2922 4957
AlwNI CAG_nnn'CTG
Cuts at: 2942 3010 4180 4444 5622 7194 2942
Size: 68 1170 264 1178 1572 3627
Fragments arranged by size:
3627 1572 1178 1170 264 68
ApaI G_GGCC'C
Cuts at: 4239 4239
Size: 7879
ApaLI G'TGCA_C
Cuts at: 5936 7092 5936
Size: 1156 6723
ApoI r'AATT_y
Cuts at: 1493 4397 5188 5414 5566 7675 1493
Size: 2904 791 226 152 2109 1697
Fragments arranged by size:
2904 2109 1697 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6433 160
Size: 872 5401 1606
Fragments arranged by size:
5401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
AvaI C'yCGr_G
Cuts at: 1870 1943 3105 3948 5204 1870
Size: 73 1162 843 1256 4545
Fragments arranged by size:
4545 1256 1162 843 73
BamHI G'GATC_C
Cuts at: 5209 5576 5209
Size: 367 7512
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 3187 3809 4749 5200
Size: 325 217 627 323 1077 622 940 451
Cuts at: 5200 6655 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1077 940 627 622 451 325
323 217
BanII G_rGCy'C
Cuts at: 1391 2418 3112 3949 4239 4491 5198 1391
Size: 1027 694 837 290 252 707 4072
Fragments arranged by size:
4072 1027 837 707 694 290 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3929 3963 6070 6104 1903
Size: 34 1992 34 2107 34 3678
Fragments arranged by size:
3678 2107 1992 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5771 6981 5771
Size: 1210 6669
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2247 2330 2933
Size: 107 122 71 631 605 575 83 603
Cuts at: 2933 3046 6490 136
Size: 113 3444 1525
Fragments arranged by size:
3444 1525 631 605 603 575 122 113
107 83 71
BglII A'GATC_T
Cuts at: 7874 7874
Size: 7879
BlpI GC'TnA_GC
Cuts at: 2412 3027 5296 2412
Size: 615 2269 4995
Fragments arranged by size:
4995 2269 615
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2321 3812 4239 4752 5940 7096 1790
Size: 316 215 1491 427 513 1188 1156 2573
Fragments arranged by size:
2573 1491 1188 1156 513 427 316 215
BmgBI CAC'GTC
Cuts at: 1318 4835 1318
Size: 3517 4362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 2320 2341
Size: 416 53 83 399 24 660 243 21
Cuts at: 2341 3815 4147 5610 6568 442
Size: 1474 332 1463 958 1753
Fragments arranged by size:
1753 1474 1463 958 660 416 399 332
243 83 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2531 3323 3812 4088 4745 6539 1239
Size: 959 333 792 489 276 657 1794 2579
Fragments arranged by size:
2579 1794 959 792 657 489 333 276
Bpu10I CC'TnA_GC
Cuts at: 3085 4213 3085
Size: 1128 6751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3311 3440 5009 5132 6004 6869 7167 3311
Size: 129 1569 123 872 865 298 4023
Fragments arranged by size:
4023 1569 872 865 298 129 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6542 882
Size: 617 5043 2219
Fragments arranged by size:
5043 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5095 5670 493
Size: 711 3891 575 2702
Fragments arranged by size:
3891 2702 711 575
BsaBI GATnn'nnATC
Cuts at: 7873 7873
Size: 7879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4416 5686 6068 6703
Size: 53 83 186 936 2883 1270 382 635
Cuts at: 6703 275
Size: 1451
Fragments arranged by size:
2883 1451 1270 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3153 3777 6312 6710 6984 7131 7542 3153
Size: 624 2535 398 274 147 411 3490
Fragments arranged by size:
3490 2535 624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 2862 2892 1178
Size: 30 165 30 1329 30 100 30 6165
Fragments arranged by size:
6165 1329 165 100 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 3074 4499 4922 1186
Size: 1192 696 1425 423 4143
Fragments arranged by size:
4143 1425 1192 696 423
BseYI C'CCAG_C
Cuts at: 2985 3176 3400 3478 4505 4678 5083 7082 7578
Size: 191 224 78 1027 173 405 1999 496
Cuts at: 7578 2985
Size: 3286
Fragments arranged by size:
3286 1999 1027 496 405 224 191 173
78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2975 2996 3143 3845 4097 4166 4406
Size: 573 1239 21 147 702 252 69 240
Cuts at: 4406 4763 1163
Size: 357 4279
Fragments arranged by size:
4279 1239 702 573 357 252 240 147
69 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5329 6090 7118 1540
Size: 275 186 3328 761 1028 2301
Fragments arranged by size:
3328 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2418 2496 3028 3933 4491 4953 5940 6025 7096
Size: 78 532 905 558 462 987 85 1071
Cuts at: 7096 2418
Size: 3201
Fragments arranged by size:
3201 1071 987 905 558 532 462 85
78
BsmI GAATG_Cn'
Cuts at: 3106 5390 5483 3106
Size: 2284 93 5502
Fragments arranged by size:
5502 2284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2321 2418 2496 3028 3112 3812
Size: 399 316 215 97 78 532 84 700
Cuts at: 3812 3933 3949 4239 4491 4752 4953 5198 5940
Size: 121 16 290 252 261 201 245 742
Cuts at: 5940 6025 7096 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 700 532 399 316 290
261 252 245 215 201 121 97 85
84 78 16
BspEI T'CCGG_A
Cuts at: 3153 7542 3153
Size: 4389 3490
BspHI T'CATG_A
Cuts at: 3231 5768 7498 3231
Size: 2537 1730 3612
Fragments arranged by size:
3612 2537 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2711 3396 3817 4378 5234 844
Size: 1520 347 685 421 561 856 3489
Fragments arranged by size:
3489 1520 856 685 561 421 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2736 4258 5136 5766 1394
Size: 429 913 1522 878 630 3507
Fragments arranged by size:
3507 1522 913 878 630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2208 3061 3826 4683 6374 6548 65
Size: 1183 960 853 765 857 1691 174 1396
Fragments arranged by size:
1691 1396 1183 960 857 853 765 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3036 4641 6523 6710 1700
Size: 110 1226 1605 1882 187 2869
Fragments arranged by size:
2869 1882 1605 1226 187 110
BsrGI T'GTAC_A
Cuts at: 96 3750 4731 96
Size: 3654 981 3244
Fragments arranged by size:
3654 3244 981
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7879
BssSI C'ACGA_G
Cuts at: 5939 6951 5939
Size: 1012 6867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3937 4180 4384 794
Size: 3143 243 204 4289
Fragments arranged by size:
4289 3143 243 204
BstBI TT'CG_AA
Cuts at: 6683 6683
Size: 7879
BstEII G'GTnAC_C
Cuts at: 1184 2674 2698 1184
Size: 1490 24 6365
Fragments arranged by size:
6365 1490 24
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2230 3727 4021 4110 2125
Size: 105 1497 294 89 5894
Fragments arranged by size:
5894 1497 294 105 89
BstYI r'GATC_y
Cuts at: 1972 3181 4422 5209 5576 5961 5978 7430 7516
Size: 1209 1241 787 367 385 17 1452 86
Cuts at: 7516 7528 7874 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1241 1209 787 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3303 3303
Size: 7879
BtgI C'CryG_G
Cuts at: 513 1065 2548 2796 2866 4704 513
Size: 552 1483 248 70 1838 3688
Fragments arranged by size:
3688 1838 1483 552 248 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7140
Fragments arranged by size:
7140 683 56
BtsI GCAGTG_nn'
Cuts at: 2146 2319 2436 3126 3275 3785 3947 4031 5395
Size: 173 117 690 149 510 162 84 1364
Cuts at: 5395 6189 6209 2146
Size: 794 20 3816
Fragments arranged by size:
3816 1364 794 690 510 173 162 149
117 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7844
DraI TTT'AAA
Cuts at: 2914 5183 2914
Size: 2269 5610
DraIII CAC_nnn'GTG
Cuts at: 2657 2657
Size: 7879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3314 6886 809
Size: 507 1998 3572 1802
Fragments arranged by size:
3572 1998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 3034 3038
Size: 54 1425 50 275 18 297 907 4
Cuts at: 3038 3717 4365 5326 6215 7844 8
Size: 679 648 961 889 1629 43
Fragments arranged by size:
1629 1425 961 907 889 679 648 297
275 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 5326 1537
Size: 275 3514 4090
Fragments arranged by size:
4090 3514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 3974 5809 1353
Size: 1250 1371 1835 3423
Fragments arranged by size:
3423 1835 1371 1250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6466 6840 6986 387
Size: 1064 138 4877 374 146 1280
Fragments arranged by size:
4877 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2416 4489 2416
Size: 2073 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2531 3323 3812 3926 4088 4745
Size: 393 566 333 792 489 114 162 657
Cuts at: 4745 5942 6539 7326 7594 1239
Size: 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 792 787 657 597 566 489
393 333 268 162 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2459 2711 3140 3323 2360
Size: 99 252 429 183 6916
Fragments arranged by size:
6916 429 252 183 99
EcoO109I rG'GnC_Cy
Cuts at: 2161 2248 2581 2816 3007 3145 3211 3316 3662
Size: 87 333 235 191 138 66 105 346
Cuts at: 3662 3871 4390 2161
Size: 209 519 5650
Fragments arranged by size:
5650 519 346 333 235 209 191 138
105 87 66
EcoRI G'AATT_C
Cuts at: 4397 5188 5566 4397
Size: 791 378 6710
Fragments arranged by size:
6710 791 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 7879
FseI GG_CCGG'CC
Cuts at: 3040 3040
Size: 7879
FspI TGC'GCA
Cuts at: 6385 6385
Size: 7879
HaeII r_GCGC'y
Cuts at: 1678 3346 4223 4455 5105 7026 1678
Size: 1668 877 232 650 1921 2531
Fragments arranged by size:
2531 1921 1668 877 650 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 2732 2764 2862
Size: 32 135 32 755 32 373 32 98
Cuts at: 2862 2894 3489 3521 4330 4362 5157 5189 6520
Size: 32 595 32 809 32 795 32 1331
Cuts at: 6520 6552 6594 6626 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1331 809 795 755 595 373 135
98 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3994 4273 5223 5469 669
Size: 988 281 2056 279 950 246 3079
Fragments arranged by size:
3079 2056 988 950 281 279 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7879
HpaI GTT'AAC
Cuts at: 5469 5469
Size: 7879
KpnI G_GTAC'C
Cuts at: 5204 5204
Size: 7879
MfeI C'AATT_G
Cuts at: 5478 5478
Size: 7879
MluI A'CGCG_T
Cuts at: 7755 7755
Size: 7879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2871 2880 4626 5016 6993 7177 1305
Size: 1566 9 1746 390 1977 184 2007
Fragments arranged by size:
2007 1977 1746 1566 390 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 3719 4367 10
Size: 54 1425 343 297 1590 648 3522
Fragments arranged by size:
3522 1590 1425 648 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 3752 5837 6196 6355 518
Size: 648 1381 1205 2085 359 159 2042
Fragments arranged by size:
2085 2042 1381 1205 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2179 2521 2989 3608 3765 4177 5015
Size: 414 159 342 468 619 157 412 838
Cuts at: 5015 5295 5972 7120 7365 1606
Size: 280 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 677 619 468 414 412
342 280 245 159 157
NaeI GCC'GGC
Cuts at: 3038 3038
Size: 7879
NcoI C'CATG_G
Cuts at: 513 1065 2796 513
Size: 552 1731 5596
Fragments arranged by size:
5596 1731 552
NdeI CA'TA_TG
Cuts at: 387 3166 4578 387
Size: 2779 1412 3688
Fragments arranged by size:
3688 2779 1412
NgoMIV G'CCGG_C
Cuts at: 3036 3036
Size: 7879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7879
NotI GC'GGCC_GC
Cuts at: 5326 5326
Size: 7879
NspI r_CATG'y
Cuts at: 1132 1229 3751 5237 6782 1132
Size: 97 2522 1486 1545 2229
Fragments arranged by size:
2522 2229 1545 1486 97
PasI CC'CwG_GG
Cuts at: 1348 3567 4978 1348
Size: 2219 1411 4249
Fragments arranged by size:
4249 2219 1411
PciI A'CATG_T
Cuts at: 6778 6778
Size: 7879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 6499
PfoI T'CCnGG_A
Cuts at: 3245 3245
Size: 7879
PmeI GTTT'AAAC
Cuts at: 5183 5183
Size: 7879
PmlI CAC'GTG
Cuts at: 5095 5095
Size: 7879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 7461 7493 5948
Size: 32 1481 32 6334
Fragments arranged by size:
6334 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2816 3145 3316 3662 3871 4390 2816
Size: 329 171 346 209 519 6305
Fragments arranged by size:
6305 519 346 329 209 171
PshAI GACnn'nnGTC
Cuts at: 1613 4476 1613
Size: 2863 5016
PsiI TTA'TAA
Cuts at: 5449 5449
Size: 7879
PspOMI G'GGCC_C
Cuts at: 4235 4235
Size: 7879
PspXI vC'TCGA_Gb
Cuts at: 3105 3105
Size: 7879
PstI C_TGCA'G
Cuts at: 830 3607 3942 4098 4380 4449 4639 4896 5231
Size: 2777 335 156 282 69 190 257 335
Cuts at: 5231 830
Size: 3478
Fragments arranged by size:
3478 2777 335 335 282 257 190 156
69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7879
PvuII CAG'CTG
Cuts at: 2179 2521 2989 3608 4177 5015 2179
Size: 342 468 619 569 838 5043
Fragments arranged by size:
5043 838 619 569 468 342
SacI G_AGCT'C
Cuts at: 2418 4491 2418
Size: 2073 5806
SalI G'TCGA_C
Cuts at: 1936 5221 1936
Size: 3285 4594
SapI GCTCTTCn'nnn_
Cuts at: 2603 2603
Size: 7879
SbfI CC_TGCA'GG
Cuts at: 5231 5231
Size: 7879
ScaI AGT'ACT
Cuts at: 4157 4844 6127 7839 4157
Size: 687 1283 1712 4197
Fragments arranged by size:
4197 1712 1283 687
SfcI C'TryA_G
Cuts at: 826 1045 3603 3938 4094 4376 4445 4635 4892
Size: 219 2558 335 156 282 69 190 257
Cuts at: 4892 5227 6362 7043 7234 826
Size: 335 1135 681 191 1471
Fragments arranged by size:
2558 1471 1135 681 335 335 282 257
219 191 190 156 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2247 3046 2247
Size: 799 7080
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7879
SmaI CCC'GGG
Cuts at: 1872 5206 1872
Size: 3334 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3105 3290 3455 5024 5111 5983
Size: 197 926 1162 185 165 1569 87 872
Cuts at: 5983 6884 7146 820
Size: 901 262 1553
Fragments arranged by size:
1569 1553 1162 926 901 872 262 197
185 165 87
SnaBI TAC'GTA
Cuts at: 493 5670 493
Size: 5177 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7879
SphI G_CATG'C
Cuts at: 1132 5237 1132
Size: 4105 3774
SspI AAT'ATT
Cuts at: 5 52 5803 5
Size: 47 5751 2081
Fragments arranged by size:
5751 2081 47
StuI AGG'CCT
Cuts at: 1517 2241 3239 3966 1517
Size: 724 998 727 5430
Fragments arranged by size:
5430 998 727 724
StyI C'CwwG_G
Cuts at: 513 1065 2281 2679 2796 2919 4056 5318 513
Size: 552 1216 398 117 123 1137 1262 3074
Fragments arranged by size:
3074 1262 1216 1137 552 398 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3205 3416 4166 4368 5906 5923
Size: 1255 939 686 211 750 202 1538 17
Cuts at: 5923 6076 6261 6706 325
Size: 153 185 445 1498
Fragments arranged by size:
1538 1498 1255 939 750 686 445 211
202 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2761 3750 4155
Size: 274 80 33 51 1443 784 989 405
Cuts at: 4155 4731 4842 6125 7837 96
Size: 576 111 1283 1712 138
Fragments arranged by size:
1712 1443 1283 989 784 576 405 274
138 111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7839 7871 7839
Size: 32 7847
Tth111I GACn'n_nGTC
Cuts at: 1316 2744 3850 1316
Size: 1428 1106 5345
Fragments arranged by size:
5345 1428 1106
XbaI T'CTAG_A
Cuts at: 4480 5215 4480
Size: 735 7144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2993 3475 3707 4702 2506
Size: 487 482 232 995 5683
Fragments arranged by size:
5683 995 487 482 232
XhoI C'TCGA_G
Cuts at: 1943 3105 1943
Size: 1162 6717
XmaI C'CCGG_G
Cuts at: 1870 5204 1870
Size: 3334 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3911 4778 6008 3416
Size: 495 867 1230 5287
Fragments arranged by size:
5287 1230 867 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5687 276
Size: 53 83 186 936 4153 2468
Fragments arranged by size:
4153 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoRV FspAI KasI NarI
NruI NsiI PacI PsrI RsrII SacII SanDI SexAI
SfoI SrfI SwaI