Restriction map for
pFN21AB4139
(Circular) (Six-base) MAPSORT of: pFN21AB4139.seq Check: 8535 from: 1 to: 6013
pFN21AB4139 6013 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2673 2673
Size: 6013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2209 3124 4076 5460 5728 1632
Size: 577 915 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 915 577 268
AfeI AGC'GCT
Cuts at: 2050 2050
Size: 6013
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 2511 3015 1166
Size: 1345 504 4164
Fragments arranged by size:
4164 1345 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 3756 5328 3756
Size: 1572 4441
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2012 2174 2418 2502 2754 2838 3006 3090
Size: 519 162 244 84 252 84 168 84
Cuts at: 3090 3174 3322 3548 3700 5809 1493
Size: 84 148 226 152 2109 1697
Fragments arranged by size:
2109 1697 519 252 244 226 168 162
152 148 84 84 84 84
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 3338 1870
Size: 73 1395 4545
Fragments arranged by size:
4545 1395 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3332 1391
Size: 1941 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2972 928
Size: 971 1073 3969
Fragments arranged by size:
3969 1073 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BfrBI ATG'CAT
Cuts at: 3036 3036
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 4074 5230 1790
Size: 2284 1156 2573
Fragments arranged by size:
2573 2284 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2700 4673 1239
Size: 1461 1973 2579
Fragments arranged by size:
2579 1973 1461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2154 2800 4138 5003 5301 2154
Size: 646 1338 865 298 2866
Fragments arranged by size:
2866 1338 865 646 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4676 882
Size: 617 3177 2219
Fragments arranged by size:
3177 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5788
Fragments arranged by size:
5788 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 3275 1186
Size: 925 1164 3924
Fragments arranged by size:
3924 1164 925
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4074 4159 5230 4074
Size: 85 1071 4857
Fragments arranged by size:
4857 1071 85
BsmI GAATG_Cn'
Cuts at: 2297 3038 3524 3617 2297
Size: 741 486 93 4693
Fragments arranged by size:
4693 741 486 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3332 4074 4159 5230 1391
Size: 399 1542 742 85 1071 2174
Fragments arranged by size:
2174 1542 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 2869 3028 3902 5632 2869
Size: 159 874 1730 3250
Fragments arranged by size:
3250 1730 874 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3368 844
Size: 1829 695 3489
Fragments arranged by size:
3489 1829 695
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 2392 2627 4508 4682 65
Size: 1183 1117 27 235 1881 174 1396
Fragments arranged by size:
1881 1396 1183 1117 235 174 27
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6013
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008
Size: 1371 367 385 17 1452 86 12 346
Cuts at: 6008 1972
Size: 1977
Fragments arranged by size:
1977 1452 1371 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2711 487
Size: 683 56 1485 3789
Fragments arranged by size:
3789 1485 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 4323 4343 3529
Size: 794 20 5199
Fragments arranged by size:
5199 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 2017 2326 3317 2017
Size: 309 991 4713
Fragments arranged by size:
4713 991 309
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978
Size: 54 1425 50 275 18 1630 889 1629
Cuts at: 5978 8
Size: 43
Fragments arranged by size:
1630 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3255 3943 1353
Size: 1902 688 3423
Fragments arranged by size:
3423 1902 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2700 3124 4076 4673 5460 5728
Size: 393 577 491 424 952 597 787 268
Cuts at: 5728 1239
Size: 1524
Fragments arranged by size:
1524 952 787 597 577 491 424 393
268
EcoRI G'AATT_C
Cuts at: 2418 2502 2754 3006 3090 3174 3322 3700 2418
Size: 84 252 252 84 84 148 378 4731
Fragments arranged by size:
4731 378 252 252 148 84 84 84
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 2052 5160 1678
Size: 374 3108 2531
Fragments arranged by size:
3108 2531 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 4654 4686 4728
Size: 32 135 32 606 32 2444 32 42
Cuts at: 4728 4760 1373
Size: 32 2626
Fragments arranged by size:
2626 2444 606 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2818 3154 748
Size: 2070 336 3607
Fragments arranged by size:
3607 2070 336
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2357 5127 5311 1305
Size: 1052 2770 184 2007
Fragments arranged by size:
2770 2007 1052 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2511 2595 2763 2874 2931 3015
Size: 648 1261 84 84 168 111 57 84
Cuts at: 3015 3099 3971 4330 4489 518
Size: 84 872 359 159 2042
Fragments arranged by size:
2042 1261 872 648 359 168 159 111
84 84 84 84 57
MspA1I CmG'CkG
Cuts at: 1606 3429 4106 5254 5499 1606
Size: 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NsiI A_TGCA'T
Cuts at: 3038 3038
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2140 3371 4916 1132
Size: 97 911 1231 1545 2229
Fragments arranged by size:
2229 1545 1231 911 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2475 2507 2475
Size: 32 5981
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 3361 4496 5177 5368 826
Size: 219 2316 1135 681 191 1471
Fragments arranged by size:
2316 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2169 2815 4117 5018 5280 820
Size: 197 926 226 646 1302 901 262 1553
Fragments arranged by size:
1553 1302 926 901 646 262 226 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 2384 2465 2549 2801 3053 3137 1517
Size: 867 81 84 252 252 84 4393
Fragments arranged by size:
4393 867 252 252 84 84 81
StyI C'CwwG_G
Cuts at: 513 1065 2004 3452 513
Size: 552 939 1448 3074
Fragments arranged by size:
3074 1448 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4040 4057 4210 4395 4840 325
Size: 1255 2460 17 153 185 445 1498
Fragments arranged by size:
2460 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2263 3137 3178 4142 2263
Size: 874 41 964 4134
Fragments arranged by size:
4134 964 874 41
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI