Restriction map for
pFN21AB4126
(Circular) (Six-base) MAPSORT of: pFN21AB4126.seq Check: 7289 from: 1 to: 6004
pFN21AB4126 6004 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 2400 4131 4504 1563
Size: 837 1731 373 3063
Fragments arranged by size:
3063 1731 837 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 2847 2923 4067 5451 5719 1632
Size: 1061 154 76 1144 1384 268 1917
Fragments arranged by size:
1917 1384 1144 1061 268 154 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2814 4903 5880 1203
Size: 1611 2089 977 1327
Fragments arranged by size:
2089 1611 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2870 3747 5319 2870
Size: 877 1572 3555
Fragments arranged by size:
3555 1572 877
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2454 2542 3122 3313 3539 3691 5800 1493
Size: 961 88 580 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 580 226 191 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3323 1391
Size: 1932 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2546 2929 928
Size: 971 647 383 4003
Fragments arranged by size:
4003 971 647 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BfrBI ATG'CAT
Cuts at: 3107 3107
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 4065 5221 1790
Size: 2275 1156 2573
Fragments arranged by size:
2573 2275 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2633 3735 4693
Size: 416 53 83 399 24 1216 1102 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1216 1102 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 4664 1239
Size: 1106 2319 2579
Fragments arranged by size:
2579 2319 1106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2138 4437 4835 5109 5256 5667 2138
Size: 2299 398 274 147 411 2475
Fragments arranged by size:
2475 2299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5779
Fragments arranged by size:
5779 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6004
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4065 4150 5221 4065
Size: 85 1071 4848
Fragments arranged by size:
4848 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3515 3608 3107
Size: 408 93 5503
Fragments arranged by size:
5503 408 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3323 4065 4150 5221 1391
Size: 399 1533 742 85 1071 2174
Fragments arranged by size:
2174 1533 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 3359 844
Size: 1608 907 3489
Fragments arranged by size:
3489 1608 907
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 2725 4808 2725
Size: 2083 3921
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2652 3223 3334 3701 4086 4103 5555 5641
Size: 680 571 111 367 385 17 1452 86
Cuts at: 5641 5653 5999 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 680 571 385 367 346 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3288 3288
Size: 6004
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2833 3520 4314 4334 2334
Size: 499 687 794 20 4004
Fragments arranged by size:
4004 794 687 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3000 3308 3000
Size: 308 5696
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3934 1353
Size: 2581 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2693 2847 2923 4067 4664 5451
Size: 393 713 348 154 76 1144 597 787
Cuts at: 5451 5719 1239
Size: 268 1524
Fragments arranged by size:
1524 1144 787 713 597 393 348 268
154 76
EcoRI G'AATT_C
Cuts at: 2454 3313 3691 2454
Size: 859 378 4767
Fragments arranged by size:
4767 859 378
EcoRV GAT'ATC
Cuts at: 2490 2490
Size: 6004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 2770 2802 4645
Size: 32 135 32 843 32 323 32 1843
Cuts at: 4645 4677 4719 4751 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1843 843 323 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2119 748
Size: 1371 4633
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 5118 5302 1305
Size: 933 2880 184 2007
Fragments arranged by size:
2880 2007 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2090 2590 3962 4321 4480 518
Size: 648 891 33 500 1372 359 159 2042
Fragments arranged by size:
2042 1372 891 648 500 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 3420 4097 5245 5490 1606
Size: 1814 677 1148 245 2120
Fragments arranged by size:
2120 1814 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5452
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NsiI A_TGCA'T
Cuts at: 3109 3109
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2038 2818 3362 4907 1132
Size: 97 809 780 544 1545 2229
Fragments arranged by size:
2229 1545 809 780 544 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 2814 4903 2814
Size: 2089 3915
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 3356 830
Size: 2526 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2301 2808 3168 3352 4487 5168 5359
Size: 219 1256 507 360 184 1135 681 191
Cuts at: 5359 826
Size: 1471
Fragments arranged by size:
1471 1256 1135 681 507 360 219 191
184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 2661 2722 3928 5
Size: 47 2609 61 1206 2081
Fragments arranged by size:
2609 2081 1206 61 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 3443 513
Size: 552 2378 3074
Fragments arranged by size:
3074 2378 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 2600 2647
Size: 274 80 33 51 1443 283 340 47
Cuts at: 2647 4250 5962 96
Size: 1603 1712 138
Fragments arranged by size:
1712 1603 1443 340 283 274 138 80
51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI