Restriction map for
pFN21AB4125
(Circular) (Six-base) MAPSORT of: pFN21AB4125.seq Check: 9012 from: 1 to: 7855
pFN21AB4125 7855 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2905 2905
Size: 7855
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5665 278
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5198 1937
Size: 3261 4594
Acc65I G'GTAC_C
Cuts at: 5176 5176
Size: 7855
AclI AA'CG_TT
Cuts at: 1563 5982 6355 1563
Size: 4419 373 3063
Fragments arranged by size:
4419 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 4707 4911 5918 7302 7570 1632
Size: 1085 1990 204 1007 1384 268 1917
Fragments arranged by size:
1990 1917 1384 1085 1007 268 204
AfeI AGC'GCT
Cuts at: 2243 2264 2243
Size: 21 7834
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7658
AflIII A'CryG_T
Cuts at: 1203 2561 2714 2729 6754 7731 1203
Size: 1358 153 15 4025 977 1327
Fragments arranged by size:
4025 1358 1327 977 153 15
AgeI A'CCGG_T
Cuts at: 3156 6686 3156
Size: 3530 4325
AhdI GACnn_n'nnGTC
Cuts at: 6584 6584
Size: 7855
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7855
AlwNI CAG_nnn'CTG
Cuts at: 3473 4022 4121 5598 7170 3473
Size: 549 99 1477 1572 4158
Fragments arranged by size:
4158 1572 1477 549 99
ApaI G_GGCC'C
Cuts at: 2073 2120 3507 2073
Size: 47 1387 6421
Fragments arranged by size:
6421 1387 47
ApaLI G'TGCA_C
Cuts at: 5912 7068 5912
Size: 1156 6699
ApoI r'AATT_y
Cuts at: 1493 2858 3334 3988 4579 4937 5045 5072 5164
Size: 1365 476 654 591 358 108 27 92
Cuts at: 5164 5390 5542 7651 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1365 654 591 476 358 226
152 108 92 27
AseI AT'TA_AT
Cuts at: 160 1032 6409 160
Size: 872 5377 1606
Fragments arranged by size:
5377 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
AvaI C'yCGr_G
Cuts at: 1870 1943 5180 1870
Size: 73 3237 4545
Fragments arranged by size:
4545 3237 73
BamHI G'GATC_C
Cuts at: 4420 5185 5552 4420
Size: 765 367 6723
Fragments arranged by size:
6723 765 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2203 3165 5176 6631
Size: 325 217 627 293 123 962 2011 1455
Cuts at: 6631 618
Size: 1842
Fragments arranged by size:
2011 1842 1455 962 627 325 293 217
123
BanII G_rGCy'C
Cuts at: 1391 2073 2120 2297 2575 3247 3507 3842 4316
Size: 682 47 177 278 672 260 335 474
Cuts at: 4316 5174 1391
Size: 858 4072
Fragments arranged by size:
4072 858 682 672 474 335 278 260
177 47
BbeI G_GCGC'C
Cuts at: 2084 2207 2084
Size: 123 7732
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2031 2065 3155 3189 6046 6080 1903
Size: 34 94 34 1090 34 2857 34 3678
Fragments arranged by size:
3678 2857 1090 94 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5747 6957 5747
Size: 1210 6645
BclI T'GATC_A
Cuts at: 1548 2808 1548
Size: 1260 6595
BfrBI ATG'CAT
Cuts at: 3085 3191 3085
Size: 106 7749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6466 136
Size: 107 122 71 631 605 4794 1525
Fragments arranged by size:
4794 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7850 7850
Size: 7855
BlpI GC'TnA_GC
Cuts at: 5272 5272
Size: 7855
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2120 3507 4538 4569 5916 7072 1790
Size: 283 47 1387 1031 31 1347 1156 2573
Fragments arranged by size:
2573 1387 1347 1156 1031 283 47 31
BmgBI CAC'GTC
Cuts at: 1318 2562 1318
Size: 1244 6611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2381 3191 4136
Size: 416 53 83 399 24 964 810 945
Cuts at: 4136 4653 5586 6544 442
Size: 517 933 958 1753
Fragments arranged by size:
1753 964 958 945 933 810 517 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7855
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4532
Size: 7855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 3398 3990 4054 4175 6515 1239
Size: 1377 782 592 64 121 2340 2579
Fragments arranged by size:
2579 2340 1377 782 592 121 64
Bpu10I CC'TnA_GC
Cuts at: 3309 3309
Size: 7855
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5980 6845 7143 5980
Size: 865 298 6692
Fragments arranged by size:
6692 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2297 6518 882
Size: 617 798 4221 2219
Fragments arranged by size:
4221 2219 798 617
BsaAI yAC'GTr
Cuts at: 493 1204 5646 493
Size: 711 4442 2702
Fragments arranged by size:
4442 2702 711
BsaBI GATnn'nnATC
Cuts at: 7849 7849
Size: 7855
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2204 5662 6044
Size: 53 83 186 936 548 123 3458 382
Cuts at: 6044 6679 275
Size: 635 1451
Fragments arranged by size:
3458 1451 936 635 548 382 186 123
83 53
BsaWI w'CCGG_w
Cuts at: 3156 3877 4192 6288 6686 6960 7107 7518 3156
Size: 721 315 2096 398 274 147 411 3493
Fragments arranged by size:
3493 2096 721 411 398 315 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3153 3183 1178
Size: 30 165 30 1750 30 5850
Fragments arranged by size:
5850 1750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3591 4340 4508 1186
Size: 2405 749 168 4533
Fragments arranged by size:
4533 2405 749 168
BseYI C'CCAG_C
Cuts at: 2259 2290 2459 7058 7554 2259
Size: 31 169 4599 496 2560
Fragments arranged by size:
4599 2560 496 169 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2427 3810 4512 1163
Size: 573 474 217 1383 702 4506
Fragments arranged by size:
4506 1383 702 573 474 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2301 5305 6066 7094 1540
Size: 275 186 300 3004 761 1028 2301
Fragments arranged by size:
3004 2301 1028 761 300 275 186
BsiHKAI G_wGCw'C
Cuts at: 2575 3842 4316 5916 6001 7072 2575
Size: 1267 474 1600 85 1071 3358
Fragments arranged by size:
3358 1600 1267 1071 474 85
BsmI GAATG_Cn'
Cuts at: 3501 5366 5459 3501
Size: 1865 93 5897
Fragments arranged by size:
5897 1865 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2120 2297 2575 3247 3507 3842
Size: 399 283 47 177 278 672 260 335
Cuts at: 3842 4316 4538 4569 5174 5916 6001 7072 1391
Size: 474 222 31 605 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 605 474 399 335
283 278 260 222 177 85 47 31
BspEI T'CCGG_A
Cuts at: 3877 7518 3877
Size: 3641 4214
BspHI T'CATG_A
Cuts at: 5744 7474 5744
Size: 1730 6125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2905 5210 844
Size: 2061 2305 3489
Fragments arranged by size:
3489 2305 2061
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 5742 1394
Size: 429 265 3654 3507
Fragments arranged by size:
3654 3507 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 6350 6524 65
Size: 1183 5102 174 1396
Fragments arranged by size:
5102 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 3156 6499 6686 1700
Size: 110 525 821 3343 187 2869
Fragments arranged by size:
3343 2869 821 525 187 110
BsrGI T'GTAC_A
Cuts at: 96 2492 4039 5101 96
Size: 2396 1547 1062 2850
Fragments arranged by size:
2850 2396 1547 1062
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7855
BssSI C'ACGA_G
Cuts at: 5915 6927 5915
Size: 1012 6843
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3082 3454 4611 794
Size: 2288 372 1157 4038
Fragments arranged by size:
4038 2288 1157 372
BstBI TT'CG_AA
Cuts at: 4165 6659 4165
Size: 2494 5361
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7855
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2836 2011
Size: 825 7030
BstYI r'GATC_y
Cuts at: 1972 2874 4420 5185 5552 5937 5954 7406 7492
Size: 902 1546 765 367 385 17 1452 86
Cuts at: 7492 7504 7850 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1546 1452 902 765 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6364
Fragments arranged by size:
6364 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3002 487
Size: 683 56 1776 5340
Fragments arranged by size:
5340 1776 683 56
BtsI GCAGTG_nn'
Cuts at: 3727 5371 6165 6185 3727
Size: 1644 794 20 5397
Fragments arranged by size:
5397 1644 794 20
ClaI AT'CG_AT
Cuts at: 1554 3831 1554
Size: 2277 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2350 2385 529
Size: 35 1786 35 5999
Fragments arranged by size:
5999 1786 35 35
DraI TTT'AAA
Cuts at: 5159 5159
Size: 7855
DraIII CAC_nnn'GTG
Cuts at: 3350 4064 3350
Size: 714 7141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 6862 809
Size: 507 1290 4256 1802
Fragments arranged by size:
4256 1802 1290 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 3946 5302
Size: 54 1425 50 275 18 468 1648 1356
Cuts at: 5302 6191 7820 8
Size: 889 1629 43
Fragments arranged by size:
1648 1629 1425 1356 889 468 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2298 5302 1537
Size: 275 486 3004 4090
Fragments arranged by size:
4090 3004 486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3879 4105 4446 5785 1353
Size: 2526 226 341 1339 3423
Fragments arranged by size:
3423 2526 1339 341 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6442 6816 6962 387
Size: 1064 138 4853 374 146 1280
Fragments arranged by size:
4853 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2573 3840 4314 2573
Size: 1267 474 6114
Fragments arranged by size:
6114 1267 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2616 2717 3398 3990 4054 4175 4707
Size: 393 984 101 681 592 64 121 532
Cuts at: 4707 4911 5918 6515 7302 7570 1239
Size: 204 1007 597 787 268 1524
Fragments arranged by size:
1524 1007 984 787 681 597 592 532
393 268 204 121 101 64
EcoNI CCTnn'n_nnAGG
Cuts at: 3008 3008
Size: 7855
EcoO109I rG'GnC_Cy
Cuts at: 2069 2070 2116 3503 4019 2069
Size: 1 46 1387 516 5905
Fragments arranged by size:
5905 1387 516 46 1
EcoRI G'AATT_C
Cuts at: 3334 4937 5045 5164 5542 3334
Size: 1603 108 119 378 5647
Fragments arranged by size:
5647 1603 378 119 108
EcoRV GAT'ATC
Cuts at: 3623 3623
Size: 7855
FspI TGC'GCA
Cuts at: 6361 6361
Size: 7855
HaeII r_GCGC'y
Cuts at: 1678 2084 2207 2245 2266 4735 7002 1678
Size: 406 123 38 21 2469 2267 2531
Fragments arranged by size:
2531 2469 2267 406 123 38 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 3824 3856 4229
Size: 32 135 32 1040 32 1180 32 373
Cuts at: 4229 4261 4379 4411 6496 6528 6570 6602 1373
Size: 32 118 32 2085 32 42 32 2626
Fragments arranged by size:
2626 2085 1180 1040 373 135 118 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4704 4842 5199 5445 669
Size: 988 281 2766 138 357 246 3079
Fragments arranged by size:
3079 2766 988 357 281 246 138
HindIII A'AGCT_T
Cuts at: 748 2787 748
Size: 2039 5816
HpaI GTT'AAC
Cuts at: 4704 5445 4704
Size: 741 7114
KasI G'GCGC_C
Cuts at: 2080 2203 2080
Size: 123 7732
KpnI G_GTAC'C
Cuts at: 5180 5180
Size: 7855
MfeI C'AATT_G
Cuts at: 3068 5454 3068
Size: 2386 5469
MluI A'CGCG_T
Cuts at: 7731 7731
Size: 7855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 2350 4057 4398 4429 6969 7153 1305
Size: 837 208 1707 341 31 2540 184 2007
Fragments arranged by size:
2540 2007 1707 837 341 208 184 31
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3948 10
Size: 54 1425 343 2116 3917
Fragments arranged by size:
3917 2116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 5813 6172 6331 518
Size: 648 843 3804 359 159 2042
Fragments arranged by size:
3804 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2343 2695 5271 5948 7096 7341 1606
Size: 439 298 352 2576 677 1148 245 2120
Fragments arranged by size:
2576 2120 1148 677 439 352 298 245
NarI GG'CG_CC
Cuts at: 2081 2204 2081
Size: 123 7732
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6364
Fragments arranged by size:
6364 939 552
NdeI CA'TA_TG
Cuts at: 387 3420 387
Size: 3033 4822
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7855
NotI GC'GGCC_GC
Cuts at: 5302 5302
Size: 7855
NsiI A_TGCA'T
Cuts at: 3087 3193 3087
Size: 106 7749
NspI r_CATG'y
Cuts at: 1132 1229 2718 2733 3080 5213 6758 1132
Size: 97 1489 15 347 2133 1545 2229
Fragments arranged by size:
2229 2133 1545 1489 347 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7855
PciI A'CATG_T
Cuts at: 2714 2729 6754 2714
Size: 15 4025 3815
Fragments arranged by size:
4025 3815 15
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3350 1108
Size: 2242 5613
PmeI GTTT'AAAC
Cuts at: 5159 5159
Size: 7855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5924 5956 7437 7469 5924
Size: 32 1481 32 6310
Fragments arranged by size:
6310 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4019 4019
Size: 7855
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7855
PsiI TTA'TAA
Cuts at: 3861 5425 3861
Size: 1564 6291
PspOMI G'GGCC_C
Cuts at: 2069 2116 3503 2069
Size: 47 1387 6421
Fragments arranged by size:
6421 1387 47
PstI C_TGCA'G
Cuts at: 830 5207 830
Size: 4377 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7855
PvuII CAG'CTG
Cuts at: 2695 2695
Size: 7855
SacI G_AGCT'C
Cuts at: 2575 3842 4316 2575
Size: 1267 474 6114
Fragments arranged by size:
6114 1267 474
SalI G'TCGA_C
Cuts at: 1936 5197 1936
Size: 3261 4594
SapI GCTCTTCn'nnn_
Cuts at: 4105 4105
Size: 7855
SbfI CC_TGCA'GG
Cuts at: 5207 5207
Size: 7855
ScaI AGT'ACT
Cuts at: 3898 6103 7815 3898
Size: 2205 1712 3938
Fragments arranged by size:
3938 2205 1712
SfcI C'TryA_G
Cuts at: 826 1045 3671 4710 5203 6338 7019 7210 826
Size: 219 2626 1039 493 1135 681 191 1471
Fragments arranged by size:
2626 1471 1135 1039 681 493 219 191
SfoI GGC'GCC
Cuts at: 2082 2205 2082
Size: 123 7732
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7855
SmaI CCC'GGG
Cuts at: 1872 5182 1872
Size: 3310 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5959 6860 7122 820
Size: 197 926 4016 901 262 1553
Fragments arranged by size:
4016 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5646 493
Size: 5153 2702
SpeI A'CTAG_T
Cuts at: 152 3785 4034 152
Size: 3633 249 3973
Fragments arranged by size:
3973 3633 249
SphI G_CATG'C
Cuts at: 1132 5213 1132
Size: 4081 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5779 5
Size: 47 3864 1863 2081
Fragments arranged by size:
3864 2081 1863 47
StuI AGG'CCT
Cuts at: 1517 4011 4252 4692 1517
Size: 2494 241 440 4680
Fragments arranged by size:
4680 2494 440 241
StyI C'CwwG_G
Cuts at: 513 1065 2004 5294 513
Size: 552 939 3290 3074
Fragments arranged by size:
3290 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 5882 5899 6052 6237 6682 325
Size: 1255 798 3504 17 153 185 445 1498
Fragments arranged by size:
3504 1498 1255 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2465 2492 2703
Size: 274 80 33 51 1443 488 27 211
Cuts at: 2703 3119 3896 4039 4358 4878 5101 6101 7813
Size: 416 777 143 319 520 223 1000 1712
Cuts at: 7813 96
Size: 138
Fragments arranged by size:
1712 1443 1000 777 520 488 416 319
274 223 211 143 138 80 51 33
27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4122 4154 7815 7847 4122
Size: 32 3661 32 4130
Fragments arranged by size:
4130 3661 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7855
XbaI T'CTAG_A
Cuts at: 5191 5191
Size: 7855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4593 4593
Size: 7855
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7855
XmaI C'CCGG_G
Cuts at: 1870 5180 1870
Size: 3310 4545
XmnI GAAnn'nnTTC
Cuts at: 2790 5001 5984 2790
Size: 2211 983 4661
Fragments arranged by size:
4661 2211 983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5663 276
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I
Bsu36I FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI