Restriction map for
pFN21AB4122
(Circular) (Six-base) MAPSORT of: pFN21AB4122.seq Check: 9286 from: 1 to: 6547
pFN21AB4122 6547 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2597 3664 3890 1937
Size: 660 1067 226 4594
Fragments arranged by size:
4594 1067 660 226
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2895 2930 3404 4610 5994 6262 1632
Size: 1263 35 474 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1206 474 268 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 5446 6423 1203
Size: 4243 977 1327
Fragments arranged by size:
4243 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2580 1184
Size: 1396 5151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3729 3761 3729
Size: 32 6515
AlwNI CAG_nnn'CTG
Cuts at: 4290 5862 4290
Size: 1572 4975
ApaI G_GGCC'C
Cuts at: 2088 2088
Size: 6547
ApaLI G'TGCA_C
Cuts at: 2055 2913 4604 5760 2055
Size: 858 1691 1156 2842
Fragments arranged by size:
2842 1691 1156 858
ApoI r'AATT_y
Cuts at: 1493 2162 2173 2287 2341 3161 3236 3856 4082
Size: 669 11 114 54 820 75 620 226
Cuts at: 4082 4234 6343 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 820 669 620 226 152 114
75 54 11
AseI AT'TA_AT
Cuts at: 160 1032 3405 5101 160
Size: 872 2373 1696 1606
Fragments arranged by size:
2373 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 2532 3872 1870
Size: 73 589 1340 4545
Fragments arranged by size:
4545 1340 589 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2524 3868 5323 618
Size: 325 217 627 737 1344 1455 1842
Fragments arranged by size:
1842 1455 1344 737 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 3866 1391
Size: 697 1778 4072
Fragments arranged by size:
4072 1778 697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 3438 928
Size: 971 790 749 4037
Fragments arranged by size:
4037 971 790 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3125 4439 5649 3125
Size: 1314 1210 4023
Fragments arranged by size:
4023 1314 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BfrBI ATG'CAT
Cuts at: 3785 3785
Size: 6547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2088 2917 4608 5764 1790
Size: 269 29 829 1691 1156 2573
Fragments arranged by size:
2573 1691 1156 829 269 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3157 4278 5236
Size: 416 53 83 399 24 1740 1121 958
Cuts at: 5236 442
Size: 1753
Fragments arranged by size:
1753 1740 1121 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3193 5207 1239
Size: 1954 2014 2579
Fragments arranged by size:
2579 2014 1954
Bpu10I CC'TnA_GC
Cuts at: 2370 2370
Size: 6547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4672 5537 5835 4672
Size: 865 298 5384
Fragments arranged by size:
5384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 5210 882
Size: 617 523 3188 2219
Fragments arranged by size:
3188 2219 617 523
BsaAI yAC'GTr
Cuts at: 493 1204 2639 4338 493
Size: 711 1435 1699 2702
Fragments arranged by size:
2702 1699 1435 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4980 5378 5652 5799 6210 4980
Size: 398 274 147 411 5317
Fragments arranged by size:
5317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3248 3278 1178
Size: 30 165 30 1845 30 4447
Fragments arranged by size:
4447 1845 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3103 3256 3477 1186
Size: 1917 153 221 4256
Fragments arranged by size:
4256 1917 221 153
BseYI C'CCAG_C
Cuts at: 5750 6246 5750
Size: 496 6051
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 3789 1163
Size: 573 837 1216 3921
Fragments arranged by size:
3921 1216 837 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 3997 4758 5786 1540
Size: 275 186 37 1959 761 1028 2301
Fragments arranged by size:
2301 1959 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 2059 2190 2620 2917 4608 4693 5764 2059
Size: 131 430 297 1691 85 1071 2842
Fragments arranged by size:
2842 1691 1071 430 297 131 85
BsmI GAATG_Cn'
Cuts at: 2766 4058 4151 2766
Size: 1292 93 5162
Fragments arranged by size:
5162 1292 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2088 2190 2620 2917 3866 4608
Size: 399 269 29 102 430 297 949 742
Cuts at: 4608 4693 5764 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 949 742 430 399 297 269
102 85 29
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 2965 3012 4436 6166 2965
Size: 47 1424 1730 3346
Fragments arranged by size:
3346 1730 1424 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3902 844
Size: 3058 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5042 5216 65
Size: 1183 3794 174 1396
Fragments arranged by size:
3794 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3791 794
Size: 2997 3550
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstYI r'GATC_y
Cuts at: 1972 2426 3877 4244 4629 4646 6098 6184 6196
Size: 454 1451 367 385 17 1452 86 12
Cuts at: 6196 6542 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1451 454 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3665 3665
Size: 6547
Bsu36I CC'TnA_GG
Cuts at: 2622 2622
Size: 6547
BtgI C'CryG_G
Cuts at: 513 1065 2032 3108 3118 513
Size: 552 967 1076 10 3942
Fragments arranged by size:
3942 1076 967 552 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2514 487
Size: 683 56 1288 4520
Fragments arranged by size:
4520 1288 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 4063 4857 4877 2355
Size: 1708 794 20 4025
Fragments arranged by size:
4025 1708 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3493 3528 529
Size: 35 2929 35 3548
Fragments arranged by size:
3548 2929 35 35
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2378 2849
Size: 54 1425 50 275 18 205 343 471
Cuts at: 2849 3994 4883 6512 8
Size: 1145 889 1629 43
Fragments arranged by size:
1629 1425 1145 889 471 343 275 205
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 3994 1537
Size: 275 223 1959 4090
Fragments arranged by size:
4090 1959 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 4477 1353
Size: 1647 1477 3423
Fragments arranged by size:
3423 1647 1477
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2895 2930 3193 3404 4610 5207 5994
Size: 393 1263 35 263 211 1206 597 787
Cuts at: 5994 6262 1239
Size: 268 1524
Fragments arranged by size:
1524 1263 1206 787 597 393 268 263
211 35
EcoO109I rG'GnC_Cy
Cuts at: 2084 2085 2084
Size: 1 6546
EcoRI G'AATT_C
Cuts at: 3161 3856 4234 3161
Size: 695 378 5474
Fragments arranged by size:
5474 695 378
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2788 2820 3191 3223 3359
Size: 32 135 32 1216 32 371 32 136
Cuts at: 3359 3391 3479 3511 3673 3705 3805 3837 5188
Size: 32 88 32 162 32 100 32 1351
Cuts at: 5188 5220 5262 5294 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1351 1216 371 162 136 135 100
88 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3134 3891 4137 669
Size: 988 281 1196 757 246 3079
Fragments arranged by size:
3079 1196 988 757 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 3716 5661 5845 1305
Size: 1084 1327 1945 184 2007
Fragments arranged by size:
2007 1945 1327 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2380 2851 10
Size: 54 1425 343 548 471 3706
Fragments arranged by size:
3706 1425 548 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4505 4864 5023 518
Size: 648 3339 359 159 2042
Fragments arranged by size:
3339 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 3803 3963 4640 5788 6033 1606
Size: 428 1769 160 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 428 245 160
NcoI C'CATG_G
Cuts at: 513 1065 3108 3118 513
Size: 552 2043 10 3942
Fragments arranged by size:
3942 2043 552 10
NdeI CA'TA_TG
Cuts at: 387 3781 387
Size: 3394 3153
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NsiI A_TGCA'T
Cuts at: 3787 3787
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 2586 3905 5450 1132
Size: 97 1357 1319 1545 2229
Fragments arranged by size:
2229 1545 1357 1319 97
PasI CC'CwG_GG
Cuts at: 1348 2114 1348
Size: 766 5781
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3129 1613
Size: 1516 5031
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 2084 2084
Size: 6547
PstI C_TGCA'G
Cuts at: 830 3802 3899 830
Size: 2972 97 3478
Fragments arranged by size:
3478 2972 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 3803 3803
Size: 6547
SacII CC_GC'GG
Cuts at: 2035 2035
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SfcI C'TryA_G
Cuts at: 826 1045 3195 3798 3895 5030 5711 5902 826
Size: 219 2150 603 97 1135 681 191 1471
Fragments arranged by size:
2150 1471 1135 681 603 219 191 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3874 1872
Size: 2002 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4651 5552 5814 820
Size: 197 926 2708 901 262 1553
Fragments arranged by size:
2708 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 2573 4471 5
Size: 47 2521 1898 2081
Fragments arranged by size:
2521 2081 1898 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 3108 3118 3986 513
Size: 552 2043 10 868 3074
Fragments arranged by size:
3074 2043 868 552 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 2058 3087 4574 4591 4744 4929
Size: 1255 460 18 1029 1487 17 153 185
Cuts at: 4929 5374 325
Size: 445 1498
Fragments arranged by size:
1498 1487 1255 1029 460 445 185 153
18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4793 6505 96
Size: 274 80 33 51 1443 2816 1712 138
Fragments arranged by size:
2816 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6507 6539 6507
Size: 32 6515
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3043
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3872 1870
Size: 2002 4545
XmnI GAAnn'nnTTC
Cuts at: 4676 4676
Size: 6547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI