Restriction map for
pFN21AB4113
(Circular) (Six-base) MAPSORT of: pFN21AB4113.seq Check: 9098 from: 1 to: 6277
pFN21AB4113 6277 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3321 3534 3620 1937
Size: 1384 213 86 4594
Fragments arranged by size:
4594 1384 213 86
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2427 3057 3456 4340 5724 5992 1632
Size: 437 358 630 399 884 1384 268 1917
Fragments arranged by size:
1917 1384 884 630 437 399 358 268
AfeI AGC'GCT
Cuts at: 2042 2042
Size: 6277
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 3336 5108 3336
Size: 1772 4505
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 2100
Size: 32 6245
AlwNI CAG_nnn'CTG
Cuts at: 4020 5592 4020
Size: 1572 4705
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2150 2175 2706 3586 3812 3964 6073 1493
Size: 657 25 531 880 226 152 2109 1697
Fragments arranged by size:
2109 1697 880 657 531 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 2213 4831 160
Size: 872 1181 2618 1606
Fragments arranged by size:
2618 1606 1181 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3596 1391
Size: 2205 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 3495 928
Size: 971 933 663 3710
Fragments arranged by size:
3710 971 933 663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 4169 5379 2272
Size: 1897 1210 3170
Fragments arranged by size:
3170 1897 1210
BclI T'GATC_A
Cuts at: 1548 3309 1548
Size: 1761 4516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2434 6272 2434
Size: 3838 2439
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 3240 4338 5494 1790
Size: 1450 1098 1156 2573
Fragments arranged by size:
2573 1450 1156 1098
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 2839 2886
Size: 416 53 83 399 24 651 771 47
Cuts at: 2886 4008 4966 442
Size: 1122 958 1753
Fragments arranged by size:
1753 1122 958 771 651 416 399 83
53 47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3084 3084
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 3434 4937 1239
Size: 951 1244 1503 2579
Fragments arranged by size:
2579 1503 1244 951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2264 3336 4710 5108 5382 5529 5940 2264
Size: 1072 1374 398 274 147 411 2601
Fragments arranged by size:
2601 1374 1072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 1178
Size: 30 165 30 697 30 5325
Fragments arranged by size:
5325 697 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6277
BseYI C'CCAG_C
Cuts at: 2519 2804 2848 5480 5976 2519
Size: 285 44 2632 496 2820
Fragments arranged by size:
2820 2632 496 285 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2229 1163
Size: 573 493 5211
Fragments arranged by size:
5211 573 493
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4338 4423 5494 4338
Size: 85 1071 5121
Fragments arranged by size:
5121 1071 85
BsiWI C'GTAC_G
Cuts at: 2929 2929
Size: 6277
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3240 3596 4338 4423 5494 1391
Size: 399 1450 356 742 85 1071 2174
Fragments arranged by size:
2174 1450 1071 742 399 356 85
BspEI T'CCGG_A
Cuts at: 2264 5940 2264
Size: 3676 2601
BspHI T'CATG_A
Cuts at: 2565 2988 4166 5896 2565
Size: 423 1178 1730 2946
Fragments arranged by size:
2946 1730 1178 423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3632 844
Size: 2788 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2313 3312 4772 4946 65
Size: 1183 1065 999 1460 174 1396
Fragments arranged by size:
1460 1396 1183 1065 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 3336 4921 5108 1700
Size: 110 204 1322 1585 187 2869
Fragments arranged by size:
2869 1585 1322 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 2281 1925
Size: 356 5921
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 3253 1184
Size: 2069 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 2962
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2434 3607 3974 4359 4376 5828 5914 5926
Size: 462 1173 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1173 462 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2082 2955 3258 3279 3348
Size: 552 939 72 6 873 303 21 69
Cuts at: 3348 513
Size: 3442
Fragments arranged by size:
3442 939 873 552 303 72 69 21
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 3011 3135 3793 4587 4607 3011
Size: 124 658 794 20 4681
Fragments arranged by size:
4681 794 658 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2296 2331 3460 3495 529
Size: 35 1732 35 1129 35 3311
Fragments arranged by size:
3311 1732 1129 35 35 35
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2548 5284 809
Size: 507 1232 2736 1802
Fragments arranged by size:
2736 1802 1232 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2862 3204
Size: 54 1425 50 275 18 249 783 342
Cuts at: 3204 3724 4613 6242 8
Size: 520 889 1629 43
Fragments arranged by size:
1629 1425 889 783 520 342 275 249
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2541 3001 4207 1353
Size: 1188 460 1206 3423
Fragments arranged by size:
3423 1206 1188 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2190 2427 3057 3434 3456 4340
Size: 393 437 121 237 630 377 22 884
Cuts at: 4340 4937 5724 5992 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 884 787 630 597 437 393 377
268 237 121 22
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 2044 5424 1678
Size: 366 3380 2531
Fragments arranged by size:
3380 2531 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3416 3448 3536 3568 4918
Size: 32 135 32 1844 32 88 32 1350
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1844 1350 135 88 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3322 3355 3442 3621 3867 669
Size: 988 281 1384 33 87 179 246 3079
Fragments arranged by size:
3079 1384 988 281 246 179 87 33
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 5391 5575 1305
Size: 975 3111 184 2007
Fragments arranged by size:
3111 2007 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2864 10
Size: 54 1425 343 1032 3423
Fragments arranged by size:
3423 1425 1032 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2960 4235 4594 4753 518
Size: 648 1794 1275 359 159 2042
Fragments arranged by size:
2042 1794 1275 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2561 2911 3030 3137 3693 4370 5518
Size: 478 477 350 119 107 556 677 1148
Cuts at: 5518 5763 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 556 478 477 350 245
119 107
NcoI C'CATG_G
Cuts at: 513 1065 2004 2955 3258 3348 513
Size: 552 939 951 303 90 3442
Fragments arranged by size:
3442 951 939 552 303 90
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2620 3635 5180 1132
Size: 97 1391 1015 1545 2229
Fragments arranged by size:
2229 1545 1391 1015 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 1108
Size: 1266 5011
PfoI T'CCnGG_A
Cuts at: 2167 3330 2167
Size: 1163 5114
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2100 2132 4346 4378 5859 5891 2100
Size: 32 2214 32 1481 32 2486
Fragments arranged by size:
2486 2214 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3350 1613
Size: 1737 4540
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PstI C_TGCA'G
Cuts at: 830 3629 830
Size: 2799 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2561 2561
Size: 6277
SacII CC_GC'GG
Cuts at: 2085 2085
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3320 3619 1936
Size: 1384 299 4594
Fragments arranged by size:
4594 1384 299
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SexAI A'CCwGG_T
Cuts at: 3048 3048
Size: 6277
SfcI C'TryA_G
Cuts at: 826 1045 3625 4760 5441 5632 826
Size: 219 2580 1135 681 191 1471
Fragments arranged by size:
2580 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 2838 4201 5
Size: 47 2786 1363 2081
Fragments arranged by size:
2786 2081 1363 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2955 3258 3348 3361 3462 3716
Size: 552 939 951 303 90 13 101 254
Cuts at: 3716 513
Size: 3074
Fragments arranged by size:
3074 951 939 552 303 254 101 90
13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2366 2381 3575 4304 4321 4474
Size: 1255 470 316 15 1194 729 17 153
Cuts at: 4474 4659 5104 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1194 729 470 445 316 185
153 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 4523 6235
Size: 274 80 33 51 1443 510 2036 1712
Cuts at: 6235 96
Size: 138
Fragments arranged by size:
2036 1712 1443 510 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 2143 3613 2143
Size: 1470 4807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2110 3409 2110
Size: 1299 4978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 2042 2450 2942 4406 2042
Size: 408 492 1464 3913
Fragments arranged by size:
3913 1464 492 408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI