Restriction map for
pFN21AB4108
(Circular) (Six-base) MAPSORT of: pFN21AB4108.seq Check: 2547 from: 1 to: 7636
pFN21AB4108 7636 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5446 278
Size: 53 83 186 936 3910 2468
Fragments arranged by size:
3910 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4731 4979 1937
Size: 2794 248 4594
Fragments arranged by size:
4594 2794 248
Acc65I G'GTAC_C
Cuts at: 4005 4957 4005
Size: 952 6684
AclI AA'CG_TT
Cuts at: 1563 3692 5763 6136 1563
Size: 2129 2071 373 3063
Fragments arranged by size:
3063 2129 2071 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 3266 3448 4206 5699 7083 7351 1632
Size: 1037 597 182 758 1493 1384 268 1917
Fragments arranged by size:
1917 1493 1384 1037 758 597 268 182
AflII C'TTAA_G
Cuts at: 820 1017 2272 820
Size: 197 1255 6184
Fragments arranged by size:
6184 1255 197
AflIII A'CryG_T
Cuts at: 1203 2999 3411 3846 6535 7512 1203
Size: 1796 412 435 2689 977 1327
Fragments arranged by size:
2689 1796 1327 977 435 412
AgeI A'CCGG_T
Cuts at: 6467 6467
Size: 7636
AhdI GACnn_n'nnGTC
Cuts at: 6365 6365
Size: 7636
AleI CACnn'nnGTG
Cuts at: 1166 2743 3428 4033 1166
Size: 1577 685 605 4769
Fragments arranged by size:
4769 1577 685 605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3696 1184
Size: 2512 5124
AlwNI CAG_nnn'CTG
Cuts at: 2692 2932 3715 5379 6951 2692
Size: 240 783 1664 1572 3377
Fragments arranged by size:
3377 1664 1572 783 240
ApaLI G'TGCA_C
Cuts at: 5693 6849 5693
Size: 1156 6480
ApoI r'AATT_y
Cuts at: 1493 2172 2417 2711 3499 3776 3911 4321 4381
Size: 679 245 294 788 277 135 410 60
Cuts at: 4381 4513 4945 5171 5323 7432 1493
Size: 132 432 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 679 432 410 294 277
245 226 152 135 132 60
AseI AT'TA_AT
Cuts at: 160 1032 2193 6190 160
Size: 872 1161 3997 1606
Fragments arranged by size:
3997 1606 1161 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
AvaI C'yCGr_G
Cuts at: 1870 1943 3985 4668 4961 1870
Size: 73 2042 683 293 4545
Fragments arranged by size:
4545 2042 683 293 73
BamHI G'GATC_C
Cuts at: 4966 5333 4966
Size: 367 7269
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 3794 4005 4957 6412
Size: 325 217 627 596 1411 211 952 1455
Cuts at: 6412 618
Size: 1842
Fragments arranged by size:
1842 1455 1411 952 627 596 325 217
211
BanII G_rGCy'C
Cuts at: 1391 2259 3738 3992 4955 1391
Size: 868 1479 254 963 4072
Fragments arranged by size:
4072 1479 963 868 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2980 3022 4114 4308 928
Size: 971 1081 42 1092 194 4256
Fragments arranged by size:
4256 1092 1081 971 194 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5827 5861 1903
Size: 34 3890 34 3678
Fragments arranged by size:
3890 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5528 6738 5528
Size: 1210 6426
BclI T'GATC_A
Cuts at: 1548 4011 1548
Size: 2463 5173
BfrBI ATG'CAT
Cuts at: 3683 3683
Size: 7636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6247 136
Size: 107 122 71 631 605 4575 1525
Fragments arranged by size:
4575 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7631 7631
Size: 7636
BlpI GC'TnA_GC
Cuts at: 4575 5053 4575
Size: 478 7158
Bme1580I G_kGCm'C
Cuts at: 1790 3388 5697 6853 1790
Size: 1598 2309 1156 2573
Fragments arranged by size:
2573 2309 1598 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2989 4769 5367
Size: 416 53 83 399 24 1572 1780 598
Cuts at: 5367 6325 442
Size: 958 1753
Fragments arranged by size:
1780 1753 1572 958 598 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3590 1056
Size: 2534 5102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2572 2986 6296 1239
Size: 1170 163 414 3310 2579
Fragments arranged by size:
3310 2579 1170 414 163
Bpu10I CC'TnA_GC
Cuts at: 2760 4694 2760
Size: 1934 5702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4664 5761 6626 6924 4123
Size: 541 1097 865 298 4835
Fragments arranged by size:
4835 1097 865 541 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2043 4712 6299 882
Size: 617 544 2669 1587 2219
Fragments arranged by size:
2669 2219 1587 617 544
BsaAI yAC'GTr
Cuts at: 493 1204 4921 5427 493
Size: 711 3717 506 2702
Fragments arranged by size:
3717 2702 711 506
BsaBI GATnn'nnATC
Cuts at: 7630 7630
Size: 7636
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5443 5825 6460 275
Size: 53 83 186 936 3910 382 635 1451
Fragments arranged by size:
3910 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6069 6467 6741 6888 7299 6069
Size: 398 274 147 411 6406
Fragments arranged by size:
6406 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2490 2520 2570 2600 3101
Size: 30 165 30 1087 30 50 30 501
Cuts at: 3101 3131 1178
Size: 30 5683
Fragments arranged by size:
5683 1087 501 165 50 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2983 1186
Size: 1030 767 5839
Fragments arranged by size:
5839 1030 767
BseYI C'CCAG_C
Cuts at: 2910 6839 7335 2910
Size: 3929 496 3211
Fragments arranged by size:
3929 3211 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7063
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5086 5847 6875 1540
Size: 275 186 3085 761 1028 2301
Fragments arranged by size:
3085 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 5697 5782 6853 2259
Size: 3438 85 1071 3042
Fragments arranged by size:
3438 3042 1071 85
BsmI GAATG_Cn'
Cuts at: 2122 2549 3543 4306 4698 5147 5240 2122
Size: 427 994 763 392 449 93 4518
Fragments arranged by size:
4518 994 763 449 427 392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 3388 3738 3992 4955 5697 5782
Size: 399 469 1129 350 254 963 742 85
Cuts at: 5782 6853 1391
Size: 1071 2174
Fragments arranged by size:
2174 1129 1071 963 742 469 399 350
254 85
BspEI T'CCGG_A
Cuts at: 7299 7299
Size: 7636
BspHI T'CATG_A
Cuts at: 5525 7255 5525
Size: 1730 5906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 4991 844
Size: 2812 1335 3489
Fragments arranged by size:
3489 2812 1335
BsrBI CCG'CTC
Cuts at: 1394 1823 5523 1394
Size: 429 3700 3507
Fragments arranged by size:
3700 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 2628 4170 4465 6131 6305 65
Size: 1183 981 399 1542 295 1666 174 1396
Fragments arranged by size:
1666 1542 1396 1183 981 399 295 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6280 6467 1700
Size: 110 4470 187 2869
Fragments arranged by size:
4470 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7636
BssSI C'ACGA_G
Cuts at: 2519 3396 5696 6708 2519
Size: 877 2300 1012 3447
Fragments arranged by size:
3447 2300 1012 877
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4433 794
Size: 3639 3997
BstBI TT'CG_AA
Cuts at: 6440 6440
Size: 7636
BstEII G'GTnAC_C
Cuts at: 1184 4688 1184
Size: 3504 4132
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 3430 4933 2797
Size: 633 1503 5500
Fragments arranged by size:
5500 1503 633
BstYI r'GATC_y
Cuts at: 1972 2647 4020 4966 5333 5718 5735 7187 7273
Size: 675 1373 946 367 385 17 1452 86
Cuts at: 7273 7285 7631 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1373 946 675 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3227 513
Size: 552 939 1223 4922
Fragments arranged by size:
4922 1223 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6897
Fragments arranged by size:
6897 683 56
BtsI GCAGTG_nn'
Cuts at: 2750 2991 3704 5152 5946 5966 2750
Size: 241 713 1448 794 20 4420
Fragments arranged by size:
4420 1448 794 713 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7636
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3787 3822 529
Size: 35 3223 35 4343
Fragments arranged by size:
4343 3223 35 35
DraI TTT'AAA
Cuts at: 2182 4940 2182
Size: 2758 4878
DraIII CAC_nnn'GTG
Cuts at: 4688 4688
Size: 7636
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3709 6643 809
Size: 507 2393 2934 1802
Fragments arranged by size:
2934 2393 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2613 2877 5083
Size: 54 1425 50 275 18 783 264 2206
Cuts at: 5083 5972 7601 8
Size: 889 1629 43
Fragments arranged by size:
2206 1629 1425 889 783 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5083 1537
Size: 275 3271 4090
Fragments arranged by size:
4090 3271 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 4197 4854 5566 1353
Size: 1371 1473 657 712 3423
Fragments arranged by size:
3423 1473 1371 712 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3291 6223 6597 6743 387
Size: 1064 138 1702 2932 374 146 1280
Fragments arranged by size:
2932 1702 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2257
Size: 7636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2572 2669 2986 3266 3448 4206
Size: 393 777 163 97 317 280 182 758
Cuts at: 4206 5699 6296 7083 7351 1239
Size: 1493 597 787 268 1524
Fragments arranged by size:
1524 1493 787 777 758 597 393 317
280 268 182 163 97
EcoO109I rG'GnC_Cy
Cuts at: 2764 3232 3512 2764
Size: 468 280 6888
Fragments arranged by size:
6888 468 280
EcoRI G'AATT_C
Cuts at: 2417 2711 4945 5323 2417
Size: 294 2234 378 4730
Fragments arranged by size:
4730 2234 378 294
EcoRV GAT'ATC
Cuts at: 4528 4528
Size: 7636
FspI TGC'GCA
Cuts at: 6142 6142
Size: 7636
HaeII r_GCGC'y
Cuts at: 1678 6783 1678
Size: 5105 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2040 2072 2583 2615 3063
Size: 32 135 32 468 32 511 32 448
Cuts at: 3063 3095 4096 4128 4771 4803 6277 6309 6351
Size: 32 1001 32 643 32 1474 32 42
Cuts at: 6351 6383 1373
Size: 32 2626
Fragments arranged by size:
2626 1474 1001 643 511 468 448 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2924 3645 4625 4980 5226 669
Size: 988 281 986 721 980 355 246 3079
Fragments arranged by size:
3079 988 986 980 721 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2275 3926 748
Size: 1527 1651 4458
Fragments arranged by size:
4458 1651 1527
HpaI GTT'AAC
Cuts at: 3645 5226 3645
Size: 1581 6055
KpnI G_GTAC'C
Cuts at: 4009 4961 4009
Size: 952 6684
MfeI C'AATT_G
Cuts at: 2235 2917 3611 4431 5235 2235
Size: 682 694 820 804 4636
Fragments arranged by size:
4636 820 804 694 682
MluI A'CGCG_T
Cuts at: 7512 7512
Size: 7636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2272 2311 2700 6750 6934 1305
Size: 967 39 389 4050 184 2007
Fragments arranged by size:
4050 2007 967 389 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 2879 10
Size: 54 1425 343 783 264 4767
Fragments arranged by size:
4767 1425 783 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2743 3428 4033 5594 5953 6112 518
Size: 648 1577 685 605 1561 359 159 2042
Fragments arranged by size:
2042 1577 1561 685 648 605 359 159
MspA1I CmG'CkG
Cuts at: 1606 2529 2929 5052 5729 6877 7122 1606
Size: 923 400 2123 677 1148 245 2120
Fragments arranged by size:
2123 2120 1148 923 677 400 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6145
Fragments arranged by size:
6145 939 552
NdeI CA'TA_TG
Cuts at: 387 3245 387
Size: 2858 4778
NheI G'CTAG_C
Cuts at: 1052 3586 1052
Size: 2534 5102
NotI GC'GGCC_GC
Cuts at: 5083 5083
Size: 7636
NsiI A_TGCA'T
Cuts at: 3685 3685
Size: 7636
NspI r_CATG'y
Cuts at: 1132 1229 2312 3003 3415 3850 4853 4994 6539
Size: 97 1083 691 412 435 1003 141 1545
Cuts at: 6539 1132
Size: 2229
Fragments arranged by size:
2229 1545 1083 1003 691 435 412 141
97
PasI CC'CwG_GG
Cuts at: 1348 4491 1348
Size: 3143 4493
PciI A'CATG_T
Cuts at: 2999 3411 3846 6535 2999
Size: 412 435 2689 4100
Fragments arranged by size:
4100 2689 435 412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7636
PfoI T'CCnGG_A
Cuts at: 3543 3543
Size: 7636
PmeI GTTT'AAAC
Cuts at: 4940 4940
Size: 7636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5705 5737 7218 7250 5705
Size: 32 1481 32 6091
Fragments arranged by size:
6091 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 3232 3512 2764
Size: 468 280 6888
Fragments arranged by size:
6888 468 280
PshAI GACnn'nnGTC
Cuts at: 1613 2829 3758 1613
Size: 1216 929 5491
Fragments arranged by size:
5491 1216 929
PsiI TTA'TAA
Cuts at: 5206 5206
Size: 7636
PstI C_TGCA'G
Cuts at: 830 3711 4636 4988 830
Size: 2881 925 352 3478
Fragments arranged by size:
3478 2881 925 352
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7636
PvuII CAG'CTG
Cuts at: 2929 2929
Size: 7636
SacI G_AGCT'C
Cuts at: 2259 2259
Size: 7636
SalI G'TCGA_C
Cuts at: 1936 4978 1936
Size: 3042 4594
SapI GCTCTTCn'nnn_
Cuts at: 2724 2724
Size: 7636
SbfI CC_TGCA'GG
Cuts at: 4988 4988
Size: 7636
ScaI AGT'ACT
Cuts at: 5884 7596 5884
Size: 1712 5924
SexAI A'CCwGG_T
Cuts at: 4140 4140
Size: 7636
SfcI C'TryA_G
Cuts at: 826 1045 2044 3707 4632 4984 6119 6800 6991
Size: 219 999 1663 925 352 1135 681 191
Cuts at: 6991 826
Size: 1471
Fragments arranged by size:
1663 1471 1135 999 925 681 352 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7636
SmaI CCC'GGG
Cuts at: 1872 4963 1872
Size: 3091 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2272 4102 4643 4668 5740 6641
Size: 197 926 329 1830 541 25 1072 901
Cuts at: 6641 6903 820
Size: 262 1553
Fragments arranged by size:
1830 1553 1072 926 901 541 329 262
197 25
SnaBI TAC'GTA
Cuts at: 493 5427 493
Size: 4934 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7636
SphI G_CATG'C
Cuts at: 1132 4994 1132
Size: 3862 3774
SspI AAT'ATT
Cuts at: 5 52 2191 3729 4654 5560 5
Size: 47 2139 1538 925 906 2081
Fragments arranged by size:
2139 2081 1538 925 906 47
StuI AGG'CCT
Cuts at: 1517 2609 4194 1517
Size: 1092 1585 4959
Fragments arranged by size:
4959 1585 1092
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 5075 513
Size: 552 939 606 2465 3074
Fragments arranged by size:
3074 2465 939 606 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4538 4922 5663 5680 5833 6018
Size: 1255 2634 324 384 741 17 153 185
Cuts at: 6018 6463 325
Size: 445 1498
Fragments arranged by size:
2634 1498 1255 741 445 384 324 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3378 3615 5882
Size: 274 80 33 51 1443 1401 237 2267
Cuts at: 5882 7594 96
Size: 1712 138
Fragments arranged by size:
2267 1712 1443 1401 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 2570 2602 3101 3133 4767 4799 7596
Size: 32 48 32 499 32 1634 32 2797
Cuts at: 7596 7628 2490
Size: 32 2498
Fragments arranged by size:
2797 2498 1634 499 48 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4901 1316
Size: 3585 4051
XbaI T'CTAG_A
Cuts at: 4972 4972
Size: 7636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 3402 2560
Size: 842 6794
XhoI C'TCGA_G
Cuts at: 1943 4668 1943
Size: 2725 4911
XmaI C'CCGG_G
Cuts at: 1870 4961 1870
Size: 3091 4545
XmnI GAAnn'nnTTC
Cuts at: 2805 5765 2805
Size: 2960 4676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5444 276
Size: 53 83 186 936 3910 2468
Fragments arranged by size:
3910 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI