Restriction map for
pFN21AB4107
(Circular) (Six-base) MAPSORT of: pFN21AB4107.seq Check: 1905 from: 1 to: 6628
pFN21AB4107 6628 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3262 3262
Size: 6628
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 3949 3949
Size: 6628
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3117 4691 6075 6343 1632
Size: 1485 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1485 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 2703 5527 6504 1203
Size: 1500 2824 977 1327
Fragments arranged by size:
2824 1500 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 2556
Size: 32 6596
AlwNI CAG_nnn'CTG
Cuts at: 4371 5943 4371
Size: 1572 5056
ApaI G_GGCC'C
Cuts at: 3363 3363
Size: 6628
ApaLI G'TGCA_C
Cuts at: 2870 4685 5841 2870
Size: 1815 1156 3657
Fragments arranged by size:
3657 1815 1156
ApoI r'AATT_y
Cuts at: 1493 2042 2864 3056 3937 4163 4315 6424 1493
Size: 549 822 192 881 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 822 549 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 2535 3953 1870
Size: 73 592 1418 4545
Fragments arranged by size:
4545 1418 592 73
BamHI G'GATC_C
Cuts at: 2149 3958 4325 2149
Size: 1809 367 4452
Fragments arranged by size:
4452 1809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 3538 3949 5404 618
Size: 325 217 627 696 1055 411 1455 1842
Fragments arranged by size:
1842 1455 1055 696 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 2362 3319 3363 3947 1391
Size: 971 957 44 584 4072
Fragments arranged by size:
4072 971 957 584 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 928
Size: 971 807 4850
Fragments arranged by size:
4850 971 807
BbvCI CC'TCA_GC
Cuts at: 2515 2515
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3277 5239 136
Size: 107 122 71 631 605 1605 1962 1525
Fragments arranged by size:
1962 1605 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2015 2674 6623 2015
Size: 659 3949 2020
Fragments arranged by size:
3949 2020 659
BlpI GC'TnA_GC
Cuts at: 2397 4045 2397
Size: 1648 4980
Bme1580I G_kGCm'C
Cuts at: 1790 2488 2874 3363 3613 3744 4689 5845 1790
Size: 698 386 489 250 131 945 1156 2573
Fragments arranged by size:
2573 1156 945 698 489 386 250 131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2198 4359 5317
Size: 416 53 83 399 24 781 2161 958
Cuts at: 5317 442
Size: 1753
Fragments arranged by size:
2161 1753 958 781 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3294 3366 3414 3477 5288 1239
Size: 2055 72 48 63 1811 2579
Fragments arranged by size:
2579 2055 1811 72 63 48
Bpu10I CC'TnA_GC
Cuts at: 2515 2515
Size: 6628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2616 4753 5618 5916 2616
Size: 2137 865 298 3328
Fragments arranged by size:
3328 2137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3325 5291 882
Size: 617 1826 1966 2219
Fragments arranged by size:
2219 1966 1826 617
BsaAI yAC'GTr
Cuts at: 493 1204 4419 493
Size: 711 3215 2702
Fragments arranged by size:
3215 2702 711
BsaBI GATnn'nnATC
Cuts at: 2020 2775 6622 2020
Size: 755 3847 2026
Fragments arranged by size:
3847 2026 755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4817 5452 275
Size: 53 83 186 936 2902 382 635 1451
Fragments arranged by size:
2902 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3772 3802 1178
Size: 30 165 30 2369 30 4004
Fragments arranged by size:
4004 2369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2616 2619 2979 3050 3309 3444 1186
Size: 1430 3 360 71 259 135 4370
Fragments arranged by size:
4370 1430 360 259 135 71 3
BseYI C'CCAG_C
Cuts at: 2487 2727 5831 6327 2487
Size: 240 3104 496 2788
Fragments arranged by size:
3104 2788 496 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2660 3471 3675 3732 1163
Size: 573 924 811 204 57 4059
Fragments arranged by size:
4059 924 811 573 204 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4078 4839 5867 1540
Size: 275 186 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2874 3319 4689 4774 5845 2874
Size: 445 1370 85 1071 3657
Fragments arranged by size:
3657 1370 1071 445 85
BsmI GAATG_Cn'
Cuts at: 2398 3348 4139 4232 2398
Size: 950 791 93 4794
Fragments arranged by size:
4794 950 791 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2362 2488 2874 3319 3363 3613 3744
Size: 399 572 126 386 445 44 250 131
Cuts at: 3744 3947 4689 4774 5845 1391
Size: 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 572 445 399 386 250
203 131 126 85 44
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 2737 3903 4517 6247 2737
Size: 1166 614 1730 3118
Fragments arranged by size:
3118 1730 1166 614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 3262 3562 3983 844
Size: 2164 254 300 421 3489
Fragments arranged by size:
3489 2164 421 300 254
BsrBI CCG'CTC
Cuts at: 1394 1823 4515 1394
Size: 429 2692 3507
Fragments arranged by size:
3507 2692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5123 5297 65
Size: 1183 3875 174 1396
Fragments arranged by size:
3875 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5272 5459 1700
Size: 110 3462 187 2869
Fragments arranged by size:
3462 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 3456 4688 5700 3456
Size: 1232 1012 4384
Fragments arranged by size:
4384 1232 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6628
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstXI CCAn_nnnn'nTGG
Cuts at: 3146 3146
Size: 6628
BstYI r'GATC_y
Cuts at: 1972 2015 2149 2674 3958 4325 4710 4727 6179
Size: 43 134 525 1284 367 385 17 1452
Cuts at: 6179 6265 6277 6623 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1284 525 385 367 346 134
86 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 2781 3033 2781
Size: 252 6376
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5137
Fragments arranged by size:
5137 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5889
Fragments arranged by size:
5889 683 56
BtsI GCAGTG_nn'
Cuts at: 3156 3744 4144 4938 4958 3156
Size: 588 400 794 20 4826
Fragments arranged by size:
4826 794 588 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3603 3638 529
Size: 35 3039 35 3519
Fragments arranged by size:
3519 3039 35 35
DraI TTT'AAA
Cuts at: 2139 3932 2139
Size: 1793 4835
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5635 809
Size: 507 4319 1802
Fragments arranged by size:
4319 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2650 4075 4964
Size: 54 1425 50 275 18 820 1425 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 820 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2348 2562 2997 4558 1353
Size: 725 270 214 435 1561 3423
Fragments arranged by size:
3423 1561 725 435 270 214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3171 3656 5215 5589 5735 387
Size: 1064 138 1582 485 1559 374 146 1280
Fragments arranged by size:
1582 1559 1280 1064 485 374 146 138
EcoICRI GAG'CTC
Cuts at: 3317 3317
Size: 6628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3117 3294 3366 3414 3477 4691 5288
Size: 393 1485 177 72 48 63 1214 597
Cuts at: 5288 6075 6343 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1485 1214 787 597 393 268 177
72 63 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3417 3417
Size: 6628
EcoO109I rG'GnC_Cy
Cuts at: 3359 3360 3359
Size: 1 6627
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FspI TGC'GCA
Cuts at: 5134 5134
Size: 6628
HaeII r_GCGC'y
Cuts at: 1678 5775 1678
Size: 4097 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2134 2166 3137 3169 3697
Size: 32 135 32 562 32 971 32 528
Cuts at: 3697 3729 3745 3777 5269 5301 5343 5375 1373
Size: 32 16 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 971 562 528 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3972 4218 669
Size: 988 281 2034 246 3079
Fragments arranged by size:
3079 2034 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 3953 3953
Size: 6628
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 2555 5742 5926 1305
Size: 1170 80 3187 184 2007
Fragments arranged by size:
3187 2007 1170 184 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4806
Fragments arranged by size:
4806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2742 3144 4586 4945 5104 518
Size: 648 1576 402 1442 359 159 2042
Fragments arranged by size:
2042 1576 1442 648 402 359 159
MspA1I CmG'CkG
Cuts at: 1606 4044 4721 5869 6114 1606
Size: 2438 677 1148 245 2120
Fragments arranged by size:
2438 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5137
Fragments arranged by size:
5137 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2405 2444 2707 3986 5531 1132
Size: 97 1176 39 263 1279 1545 2229
Fragments arranged by size:
2229 1545 1279 1176 263 97 39
PasI CC'CwG_GG
Cuts at: 1348 3563 3596 1348
Size: 2215 33 4380
Fragments arranged by size:
4380 2215 33
PciI A'CATG_T
Cuts at: 2703 5527 2703
Size: 2824 3804
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2480 3242 3596 1108
Size: 1372 762 354 4140
Fragments arranged by size:
4140 1372 762 354
PfoI T'CCnGG_A
Cuts at: 2463 3310 3382 2463
Size: 847 72 5709
Fragments arranged by size:
5709 847 72
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PspOMI G'GGCC_C
Cuts at: 3359 3359
Size: 6628
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2265 2297 2265
Size: 32 6596
PstI C_TGCA'G
Cuts at: 830 2134 3980 830
Size: 1304 1846 3478
Fragments arranged by size:
3478 1846 1304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
SacI G_AGCT'C
Cuts at: 3319 3319
Size: 6628
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 2235 2815 4876 6588 2235
Size: 580 2061 1712 2275
Fragments arranged by size:
2275 2061 1712 580
SfcI C'TryA_G
Cuts at: 826 1045 2130 3285 3976 5111 5792 5983 826
Size: 219 1085 1155 691 1135 681 191 1471
Fragments arranged by size:
1471 1155 1135 1085 691 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3277 3277
Size: 6628
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2631 4732 5633 5895 820
Size: 197 926 688 2101 901 262 1553
Fragments arranged by size:
2101 1553 926 901 688 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 2375 4552 5
Size: 47 2323 2177 2081
Fragments arranged by size:
2323 2177 2081 47
StuI AGG'CCT
Cuts at: 1517 3291 1517
Size: 1774 4854
StyI C'CwwG_G
Cuts at: 513 1065 2004 3470 3547 3636 3717 4067 513
Size: 552 939 1466 77 89 81 350 3074
Fragments arranged by size:
3074 1466 939 552 350 89 81 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3631 3856 4655 4672 4825 5010 5455
Size: 1255 2051 225 799 17 153 185 445
Cuts at: 5455 325
Size: 1498
Fragments arranged by size:
2051 1498 1255 799 445 225 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2233 2813 3871
Size: 274 80 33 51 1443 256 580 1058
Cuts at: 3871 4874 6586 96
Size: 1003 1712 138
Fragments arranged by size:
1712 1443 1058 1003 580 274 256 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 6588 6620 2484
Size: 32 4072 32 2492
Fragments arranged by size:
4072 2492 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6628
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 2277 4757 2277
Size: 2480 4148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PvuII RsrII SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI