Restriction map for
pFN21AB4102
(Circular) (Six-base) MAPSORT of: pFN21AB4102.seq Check: 8689 from: 1 to: 6850
pFN21AB4102 6850 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3684 3684
Size: 6850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4660 278
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4193 1937
Size: 2256 4594
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 4977 5350 1563
Size: 3414 373 3063
Fragments arranged by size:
3414 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 3282 4913 6297 6565 1632
Size: 684 966 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 966 684 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6653
AflIII A'CryG_T
Cuts at: 1203 2022 3843 5749 6726 1203
Size: 819 1821 1906 977 1327
Fragments arranged by size:
1906 1821 1327 977 819
AgeI A'CCGG_T
Cuts at: 5681 5681
Size: 6850
AhdI GACnn_n'nnGTC
Cuts at: 5579 5579
Size: 6850
AleI CACnn'nnGTG
Cuts at: 1166 2744 3416 4078 1166
Size: 1578 672 662 3938
Fragments arranged by size:
3938 1578 672 662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3107 1184
Size: 1923 4927
AlwNI CAG_nnn'CTG
Cuts at: 2179 2290 2786 2834 2894 3127 3260 3686 4593
Size: 111 496 48 60 233 133 426 907
Cuts at: 4593 6165 2179
Size: 1572 2864
Fragments arranged by size:
2864 1572 907 496 426 233 133 111
60 48
ApaLI G'TGCA_C
Cuts at: 4907 6063 4907
Size: 1156 5694
ApoI r'AATT_y
Cuts at: 1493 4121 4159 4385 4537 6646 1493
Size: 2628 38 226 152 2109 1697
Fragments arranged by size:
2628 2109 1697 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 5404 160
Size: 872 4372 1606
Fragments arranged by size:
4372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 4175 1870
Size: 73 2232 4545
Fragments arranged by size:
4545 2232 73
BamHI G'GATC_C
Cuts at: 4180 4547 4180
Size: 367 6483
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2732 3019 3377 4171 5626
Size: 325 217 627 945 287 358 794 1455
Cuts at: 5626 618
Size: 1842
Fragments arranged by size:
1842 1455 945 794 627 358 325 287
217
BanII G_rGCy'C
Cuts at: 1391 2043 4169 1391
Size: 652 2126 4072
Fragments arranged by size:
4072 2126 652
BbeI G_GCGC'C
Cuts at: 3381 3381
Size: 6850
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3508 928
Size: 971 1609 4270
Fragments arranged by size:
4270 1609 971
BbvCI CC'TCA_GC
Cuts at: 2827 2895 3080 2827
Size: 68 185 6597
Fragments arranged by size:
6597 185 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3436 3470 3553 3587 4095 4129 5041
Size: 34 1499 34 83 34 508 34 912
Cuts at: 5041 5075 1903
Size: 34 3678
Fragments arranged by size:
3678 1499 912 508 83 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 5952 4742
Size: 1210 5640
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6850
BfrBI ATG'CAT
Cuts at: 3976 3976
Size: 6850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 2175 2219
Size: 107 122 71 631 605 448 55 44
Cuts at: 2219 2567 3100 3317 5461 136
Size: 348 533 217 2144 1525
Fragments arranged by size:
2144 1525 631 605 533 448 348 217
122 107 71 55 44
BglII A'GATC_T
Cuts at: 3735 6845 3735
Size: 3110 3740
BlpI GC'TnA_GC
Cuts at: 4267 4267
Size: 6850
Bme1580I G_kGCm'C
Cuts at: 1790 2724 2910 3190 3540 3566 4911 6067 1790
Size: 934 186 280 350 26 1345 1156 2573
Fragments arranged by size:
2573 1345 1156 934 350 280 186 26
BmgBI CAC'GTC
Cuts at: 1318 3844 1318
Size: 2526 4324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2445 3571 4072
Size: 416 53 83 399 24 1028 1126 501
Cuts at: 4072 4581 5539 442
Size: 509 958 1753
Fragments arranged by size:
1753 1126 1028 958 509 501 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3982 4087 3982
Size: 105 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2201 2267 2323 2656 2677 3149 5510
Size: 941 21 66 56 333 21 472 2361
Cuts at: 5510 1239
Size: 2579
Fragments arranged by size:
2579 2361 941 472 333 66 56 21
21
Bpu10I CC'TnA_GC
Cuts at: 2827 2895 2960 3080 3713 2827
Size: 68 65 120 633 5964
Fragments arranged by size:
5964 633 120 68 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3350 3812 4975 5840 6138 3350
Size: 462 1163 865 298 4062
Fragments arranged by size:
4062 1163 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 2303 2544 4061 5513 882
Size: 617 564 240 241 1517 1452 2219
Fragments arranged by size:
2219 1517 1452 617 564 241 240
BsaAI yAC'GTr
Cuts at: 493 1204 2146 4641 493
Size: 711 942 2495 2702
Fragments arranged by size:
2702 2495 942 711
BsaBI GATnn'nnATC
Cuts at: 6844 6844
Size: 6850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3168 3378 4657 5039
Size: 53 83 186 936 1635 210 1279 382
Cuts at: 5039 5674 275
Size: 635 1451
Fragments arranged by size:
1635 1451 1279 936 635 382 210 186
83 53
BsaWI w'CCGG_w
Cuts at: 2301 5283 5681 5955 6102 6513 2301
Size: 2982 398 274 147 411 2638
Fragments arranged by size:
2982 2638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3519 3549 1178
Size: 30 165 30 2116 30 4479
Fragments arranged by size:
4479 2116 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 2894 3044 3242 3353 4076 4079 1186
Size: 1434 274 150 198 111 723 3 3957
Fragments arranged by size:
3957 1434 723 274 198 150 111 3
BseYI C'CCAG_C
Cuts at: 2423 3171 4132 4145 6053 6549 2423
Size: 748 961 13 1908 496 2724
Fragments arranged by size:
2724 1908 961 748 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3406 3656 1163
Size: 573 1670 250 4357
Fragments arranged by size:
4357 1670 573 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4300 5061 6089 1540
Size: 275 186 2299 761 1028 2301
Fragments arranged by size:
2301 2299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 4911 4996 6067 3558
Size: 1353 85 1071 4341
Fragments arranged by size:
4341 1353 1071 85
BsmI GAATG_Cn'
Cuts at: 4361 4454 4361
Size: 93 6757
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2129
Size: 6850
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2724 2910 3190 3540 3558 3566
Size: 399 253 681 186 280 350 18 8
Cuts at: 3566 4169 4911 4996 6067 1391
Size: 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 603 399 350 280
253 186 85 18 8
BspEI T'CCGG_A
Cuts at: 6513 6513
Size: 6850
BspHI T'CATG_A
Cuts at: 4739 6469 4739
Size: 1730 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 3037 3094 3660 3678 3684 4011 4038
Size: 1325 868 57 566 18 6 327 27
Cuts at: 4038 4205 844
Size: 167 3489
Fragments arranged by size:
3489 1325 868 566 327 167 57 27
18 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2434 4737 1394
Size: 429 208 403 2303 3507
Fragments arranged by size:
3507 2303 429 403 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 5345 5519 65
Size: 1183 4097 174 1396
Fragments arranged by size:
4097 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3022 4017 5494 5681 1700
Size: 110 1212 995 1477 187 2869
Fragments arranged by size:
2869 1477 1212 995 187 110
BsrGI T'GTAC_A
Cuts at: 96 2224 96
Size: 2128 4722
BssHII G'CGCG_C
Cuts at: 1925 2013 2097 2123 1925
Size: 88 84 26 6652
Fragments arranged by size:
6652 88 84 26
BssSI C'ACGA_G
Cuts at: 4910 5922 4910
Size: 1012 5838
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6850
BstBI TT'CG_AA
Cuts at: 5654 5654
Size: 6850
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6850
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2050
Size: 6850
BstYI r'GATC_y
Cuts at: 1972 2490 3735 4180 4547 4932 4949 6401 6487
Size: 518 1245 445 367 385 17 1452 86
Cuts at: 6487 6499 6845 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1245 518 445 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3097 3097
Size: 6850
BtgI C'CryG_G
Cuts at: 513 1065 2061 3510 3522 513
Size: 552 996 1449 12 3841
Fragments arranged by size:
3841 1449 996 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3907 487
Size: 683 56 2681 3430
Fragments arranged by size:
3430 2681 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 3347 3540 4366 5160 5180 2134
Size: 1213 193 826 794 20 3804
Fragments arranged by size:
3804 1213 826 794 193 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6815
DraI TTT'AAA
Cuts at: 4154 4154
Size: 6850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3695 5857 809
Size: 507 2379 2162 1802
Fragments arranged by size:
2379 2162 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2584 2982
Size: 54 1425 50 275 18 282 472 398
Cuts at: 2982 3024 3507 3858 4297 5186 6815 8
Size: 42 483 351 439 889 1629 43
Fragments arranged by size:
1629 1425 889 483 472 439 398 351
282 275 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 4297 1537
Size: 275 2485 4090
Fragments arranged by size:
4090 2485 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4780 1353
Size: 3427 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 5437 5811 5957 387
Size: 1064 138 462 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2201 2267 2316 2323 2656 2677
Size: 393 548 21 66 49 7 333 21
Cuts at: 2677 3149 3282 4913 5510 6297 6565 1239
Size: 472 133 1631 597 787 268 1524
Fragments arranged by size:
1631 1524 787 597 548 472 393 333
268 133 66 49 21 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 4058 2183
Size: 1875 4975
EcoO109I rG'GnC_Cy
Cuts at: 2176 2327 2560 3049 3076 2176
Size: 151 233 489 27 5950
Fragments arranged by size:
5950 489 233 151 27
EcoRI G'AATT_C
Cuts at: 4121 4159 4537 4121
Size: 38 378 6434
Fragments arranged by size:
6434 378 38
FspI TGC'GCA
Cuts at: 3788 3953 5356 3788
Size: 165 1403 5282
Fragments arranged by size:
5282 1403 165
FspAI rTGC'GCAy
Cuts at: 3788 3788
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 2548 3381 5997 1678
Size: 870 833 2616 2531
Fragments arranged by size:
2616 2531 870 833
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 3284 3316 5491
Size: 32 135 32 728 32 952 32 2175
Cuts at: 5491 5523 5565 5597 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2175 952 728 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4194 4440 669
Size: 988 281 2256 246 3079
Fragments arranged by size:
3079 2256 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KasI G'GCGC_C
Cuts at: 3377 3377
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 4449 4449
Size: 6850
MluI A'CGCG_T
Cuts at: 2022 6726 2022
Size: 4704 2146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5964 6148 1305
Size: 4659 184 2007
Fragments arranged by size:
4659 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 2984 3509 10
Size: 54 1425 343 754 398 525 3351
Fragments arranged by size:
3351 1425 754 525 398 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2382 2744 2996 3416 3726 3887
Size: 648 1071 145 362 252 420 310 161
Cuts at: 3887 3911 4078 4808 5167 5326 518
Size: 24 167 730 359 159 2042
Fragments arranged by size:
2042 1071 730 648 420 362 359 310
252 167 161 159 145 24
MspA1I CmG'CkG
Cuts at: 1606 2063 2625 2697 2894 3216 3631 3683 3866
Size: 457 562 72 197 322 415 52 183
Cuts at: 3866 4266 4943 6091 6336 1606
Size: 400 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 562 457 415 400 322
245 197 183 72 52
NaeI GCC'GGC
Cuts at: 3024 3024
Size: 6850
NarI GG'CG_CC
Cuts at: 3378 3378
Size: 6850
NcoI C'CATG_G
Cuts at: 513 1065 3510 3522 513
Size: 552 2445 12 3841
Fragments arranged by size:
3841 2445 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6850
NgoMIV G'CCGG_C
Cuts at: 3022 3022
Size: 6850
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6850
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NsiI A_TGCA'T
Cuts at: 3978 3978
Size: 6850
NspI r_CATG'y
Cuts at: 1132 1229 3457 3980 4208 5753 1132
Size: 97 2228 523 228 1545 2229
Fragments arranged by size:
2229 2228 1545 523 228 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 5347
PciI A'CATG_T
Cuts at: 5749 5749
Size: 6850
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 1108
Size: 1272 5578
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 6432 6464 4919
Size: 32 1481 32 5305
Fragments arranged by size:
5305 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3049 3076 3049
Size: 27 6823
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6850
PsiI TTA'TAA
Cuts at: 4420 4420
Size: 6850
PstI C_TGCA'G
Cuts at: 830 2175 2395 2800 3840 4008 4035 4202 830
Size: 1345 220 405 1040 168 27 167 3478
Fragments arranged by size:
3478 1345 1040 405 220 168 167 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 2697 2894 3631 3683 3866 2697
Size: 197 737 52 183 5681
Fragments arranged by size:
5681 737 197 183 52
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 6850
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SbfI CC_TGCA'GG
Cuts at: 2175 3840 4035 4202 2175
Size: 1665 195 167 4823
Fragments arranged by size:
4823 1665 195 167
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SfcI C'TryA_G
Cuts at: 826 1045 2171 2391 2796 3435 3836 3981 4004
Size: 219 1126 220 405 639 401 145 23
Cuts at: 4004 4031 4198 5333 6014 6205 826
Size: 27 167 1135 681 191 1471
Fragments arranged by size:
1471 1135 1126 681 639 405 401 220
219 191 167 145 27 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 2567 3317 2567
Size: 750 6100
SfoI GGC'GCC
Cuts at: 3379 3379
Size: 6850
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 4177 1872
Size: 2305 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3365 3827 4954 5855 6117 820
Size: 197 926 1422 462 1127 901 262 1553
Fragments arranged by size:
1553 1422 1127 926 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 3980 4208 1132
Size: 2848 228 3774
Fragments arranged by size:
3774 2848 228
SspI AAT'ATT
Cuts at: 5 52 4774 5
Size: 47 4722 2081
Fragments arranged by size:
4722 2081 47
StuI AGG'CCT
Cuts at: 1517 2481 3014 1517
Size: 964 533 5353
Fragments arranged by size:
5353 964 533
StyI C'CwwG_G
Cuts at: 513 1065 2772 3312 3510 3522 3612 4289 513
Size: 552 1707 540 198 12 90 677 3074
Fragments arranged by size:
3074 1707 677 552 540 198 90 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2533 2719 2750 4877 4894 5047 5232
Size: 1255 953 186 31 2127 17 153 185
Cuts at: 5232 5677 325
Size: 445 1498
Fragments arranged by size:
2127 1498 1255 953 445 186 185 153
31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 2256 5096
Size: 274 80 33 51 1443 247 32 2840
Cuts at: 5096 6808 96
Size: 1712 138
Fragments arranged by size:
2840 1712 1443 274 247 138 80 51
33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3519 3551 3807 3839 6810 6842 3519
Size: 32 256 32 2971 32 3527
Fragments arranged by size:
3527 2971 256 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2314 1316
Size: 998 5852
XbaI T'CTAG_A
Cuts at: 4186 4186
Size: 6850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3388 3388
Size: 6850
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6850
XmaI C'CCGG_G
Cuts at: 1870 4175 1870
Size: 2305 4545
XmnI GAAnn'nnTTC
Cuts at: 4979 4979
Size: 6850
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4658 276
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SrfI SwaI