Restriction map for
pFN21AB4098
(Circular) (Six-base) MAPSORT of: pFN21AB4098.seq Check: 2868 from: 1 to: 5938
pFN21AB4098 5938 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2238 4001 5385 5653 1632
Size: 599 7 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 599 268 7
AfeI AGC'GCT
Cuts at: 2035 2035
Size: 5938
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2880 2912 2880
Size: 32 5906
AlwNI CAG_nnn'CTG
Cuts at: 2125 2290 3681 5253 2125
Size: 165 1391 1572 2810
Fragments arranged by size:
2810 1572 1391 165
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 2306 2702 3247 3473 3625 5734 1493
Size: 813 396 545 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 545 396 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 3263 1870
Size: 73 124 1196 4545
Fragments arranged by size:
4545 1196 124 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3175 3208 3175
Size: 33 5905
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2062 3259 4714 618
Size: 325 217 627 264 11 1197 1455 1842
Fragments arranged by size:
1842 1455 1197 627 325 264 217 11
BanII G_rGCy'C
Cuts at: 1391 3173 3257 1391
Size: 1782 84 4072
Fragments arranged by size:
4072 1782 84
BbeI G_GCGC'C
Cuts at: 2055 2055
Size: 5938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2865 3099 928
Size: 971 966 234 3767
Fragments arranged by size:
3767 971 966 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 3830 5040 3203
Size: 627 1210 4101
Fragments arranged by size:
4101 1210 627
BclI T'GATC_A
Cuts at: 1548 2650 1548
Size: 1102 4836
BfrBI ATG'CAT
Cuts at: 2147 2147
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4549 136
Size: 107 122 71 631 605 337 2540 1525
Fragments arranged by size:
2540 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3043 5933 3043
Size: 2890 3048
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2067 3999 5155 1790
Size: 277 1932 1156 2573
Fragments arranged by size:
2573 1932 1156 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 3669 4627
Size: 416 53 83 399 24 1027 1225 958
Cuts at: 4627 442
Size: 1753
Fragments arranged by size:
1753 1225 1027 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2655 4598 1239
Size: 1416 1943 2579
Fragments arranged by size:
2579 1943 1416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 3048 5932 3048
Size: 2884 3054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 3745 4127 4762
Size: 53 83 186 936 519 1693 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1693 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2880 2910 1178
Size: 30 165 30 1477 30 4206
Fragments arranged by size:
4206 1477 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2901 1186
Size: 1715 4223
BseYI C'CCAG_C
Cuts at: 5141 5637 5141
Size: 496 5442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2928 1163
Size: 573 1192 4173
Fragments arranged by size:
4173 1192 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3173 3999 4084 5155 3173
Size: 826 85 1071 3956
Fragments arranged by size:
3956 1071 826 85
BsmI GAATG_Cn'
Cuts at: 2782 2980 3449 3542 2782
Size: 198 469 93 5178
Fragments arranged by size:
5178 469 198 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 3173 3257 3999 4084 5155 1391
Size: 399 277 1106 84 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 742 399 277 85 84
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3293 844
Size: 2449 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4433 4607 65
Size: 1183 1539 1646 174 1396
Fragments arranged by size:
1646 1539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 4582 4769 1700
Size: 110 244 2528 187 2869
Fragments arranged by size:
2869 2528 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 2438 96
Size: 2342 3596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 2374 4742 2374
Size: 2368 3570
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 3043 3268 3635 4020 4037 5489 5575 5587
Size: 1071 225 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1071 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3234 513
Size: 552 2169 3217
Fragments arranged by size:
3217 2169 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 2616 2940 3454 4248 4268 2616
Size: 324 514 794 20 4286
Fragments arranged by size:
4286 794 514 324 20
ClaI AT'CG_AT
Cuts at: 1554 2485 1554
Size: 931 5007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3210 3245 529
Size: 35 2646 35 3222
Fragments arranged by size:
3222 2646 35 35
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3231 3385 4274
Size: 54 1425 50 275 18 1401 154 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1401 889 275 154 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2257 3868 1353
Size: 904 1611 3423
Fragments arranged by size:
3423 1611 904
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3171 3171
Size: 5938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2238 2655 4001 4598 5385 5653
Size: 393 599 7 417 1346 597 787 268
Cuts at: 5653 1239
Size: 1524
Fragments arranged by size:
1524 1346 787 599 597 417 393 268
7
EcoRI G'AATT_C
Cuts at: 2306 3247 3625 2306
Size: 941 378 4619
Fragments arranged by size:
4619 941 378
EcoRV GAT'ATC
Cuts at: 2224 2224
Size: 5938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2178 2879 3166 2178
Size: 701 287 4950
Fragments arranged by size:
4950 701 287
FspI TGC'GCA
Cuts at: 2030 4444 2030
Size: 2414 3524
HaeII r_GCGC'y
Cuts at: 1678 2037 2055 5085 1678
Size: 359 18 3030 2531
Fragments arranged by size:
3030 2531 359 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4579 4611 4653 4685 1373
Size: 32 135 32 3007 32 42 32 2626
Fragments arranged by size:
3007 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2446 3282 3528 669
Size: 988 281 508 836 246 3079
Fragments arranged by size:
3079 988 836 508 281 246
HindIII A'AGCT_T
Cuts at: 748 2681 748
Size: 1933 4005
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KasI G'GCGC_C
Cuts at: 2051 2051
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 2261 3537 2261
Size: 1276 4662
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3233 10
Size: 54 1425 343 1401 2715
Fragments arranged by size:
2715 1425 1401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 3896 4255 4414 518
Size: 648 1222 1508 359 159 2042
Fragments arranged by size:
2042 1508 1222 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3354 4031 5179 5424 1606
Size: 501 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 501 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 5938
NarI GG'CG_CC
Cuts at: 2052 2052
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 3234 513
Size: 552 2169 3217
Fragments arranged by size:
3217 2169 552
NdeI CA'TA_TG
Cuts at: 387 2754 387
Size: 2367 3571
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NsiI A_TGCA'T
Cuts at: 2149 2149
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2151 3296 4841 1132
Size: 97 922 1145 1545 2229
Fragments arranged by size:
2229 1545 1145 922 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5938
PfoI T'CCnGG_A
Cuts at: 2969 2969
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2880 2912 4007 4039 5520 5552 2880
Size: 32 1095 32 1481 32 3266
Fragments arranged by size:
3266 1481 1095 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 3290 830
Size: 2460 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
SacI G_AGCT'C
Cuts at: 3173 3173
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 3032 4186 5898 3032
Size: 1154 1712 3072
Fragments arranged by size:
3072 1712 1154
SfcI C'TryA_G
Cuts at: 826 1045 2092 3117 3286 4421 5102 5293 826
Size: 219 1047 1025 169 1135 681 191 1471
Fragments arranged by size:
1471 1135 1047 1025 681 219 191 169
SfoI GGC'GCC
Cuts at: 2053 2053
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 2474 3862 5
Size: 47 2422 1388 2081
Fragments arranged by size:
2422 2081 1388 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 3234 3377 513
Size: 552 2169 143 3074
Fragments arranged by size:
3074 2169 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3965 3982 4135 4320 4765 325
Size: 1255 2385 17 153 185 445 1498
Fragments arranged by size:
2385 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2438 3030 4184
Size: 274 80 33 51 1443 461 592 1154
Cuts at: 4184 5896 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1154 592 461 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2632 2664 5898 5930 2632
Size: 32 3234 32 2640
Fragments arranged by size:
3234 2640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2450 1316
Size: 1134 4804
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 3126 4067 3126
Size: 941 4997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbvCI Bpu10I BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI