Restriction map for
pFN21AB4095
(Circular) (Six-base) MAPSORT of: pFN21AB4095.seq Check: 2722 from: 1 to: 7954
pFN21AB4095 7954 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4217 4217
Size: 7954
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5764 278
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 5297 1937
Size: 145 3215 4594
Fragments arranged by size:
4594 3215 145
Acc65I G'GTAC_C
Cuts at: 5275 5275
Size: 7954
AclI AA'CG_TT
Cuts at: 1563 6081 6454 1563
Size: 4518 373 3063
Fragments arranged by size:
4518 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 3545 5060 6017 7401 7669 1632
Size: 1295 618 1515 957 1384 268 1917
Fragments arranged by size:
1917 1515 1384 1295 957 618 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7757
AflIII A'CryG_T
Cuts at: 1203 3369 3678 6853 7830 1203
Size: 2166 309 3175 977 1327
Fragments arranged by size:
3175 2166 1327 977 309
AgeI A'CCGG_T
Cuts at: 6785 6785
Size: 7954
AhdI GACnn_n'nnGTC
Cuts at: 4588 6683 4588
Size: 2095 5859
AleI CACnn'nnGTG
Cuts at: 1166 4017 4247 1166
Size: 2851 230 4873
Fragments arranged by size:
4873 2851 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4025 4035 1184
Size: 2841 10 5103
Fragments arranged by size:
5103 2841 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2885 2917 4543 4575 2885
Size: 32 1626 32 6264
Fragments arranged by size:
6264 1626 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2500 3007 3673 4926 5697 7269 2500
Size: 507 666 1253 771 1572 3185
Fragments arranged by size:
3185 1572 1253 771 666 507
ApaI G_GGCC'C
Cuts at: 4140 4140
Size: 7954
ApaLI G'TGCA_C
Cuts at: 6011 7167 6011
Size: 1156 6798
ApoI r'AATT_y
Cuts at: 1493 3484 5263 5489 5641 7750 1493
Size: 1991 1779 226 152 2109 1697
Fragments arranged by size:
2109 1991 1779 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6508 160
Size: 872 5476 1606
Fragments arranged by size:
5476 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
AvaI C'yCGr_G
Cuts at: 1870 1943 3537 4264 4825 5279 1870
Size: 73 1594 727 561 454 4545
Fragments arranged by size:
4545 1594 727 561 454 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4965 4998 4965
Size: 33 7921
BamHI G'GATC_C
Cuts at: 2518 3619 5284 5651 2518
Size: 1101 1665 367 4821
Fragments arranged by size:
4821 1665 1101 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2641 2755 4260 4735 5275
Size: 325 217 627 854 114 1505 475 540
Cuts at: 5275 6730 618
Size: 1455 1842
Fragments arranged by size:
1842 1505 1455 854 627 540 475 325
217 114
BanII G_rGCy'C
Cuts at: 1391 2359 2475 2886 3291 3938 4140 4256 4524
Size: 968 116 411 405 647 202 116 268
Cuts at: 4524 4832 4864 5273 1391
Size: 308 32 409 4072
Fragments arranged by size:
4072 968 647 411 409 405 308 268
202 116 116 32
BbeI G_GCGC'C
Cuts at: 4264 4739 4264
Size: 475 7479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6983
BbvCI CC'TCA_GC
Cuts at: 3314 4488 3314
Size: 1174 6780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6145 6179 1903
Size: 34 4208 34 3678
Fragments arranged by size:
4208 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4966 5846 7056 4966
Size: 880 1210 5864
Fragments arranged by size:
5864 1210 880
BclI T'GATC_A
Cuts at: 1548 3135 1548
Size: 1587 6367
BfrBI ATG'CAT
Cuts at: 3517 3517
Size: 7954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2223 3099 4056
Size: 107 122 71 631 605 551 876 957
Cuts at: 4056 4269 4749 4811 4836 6565 136
Size: 213 480 62 25 1729 1525
Fragments arranged by size:
1729 1525 957 876 631 605 551 480
213 122 107 71 62 25
BglII A'GATC_T
Cuts at: 7949 7949
Size: 7954
BlpI GC'TnA_GC
Cuts at: 5371 5371
Size: 7954
Bme1580I G_kGCm'C
Cuts at: 1790 2760 4140 6015 7171 1790
Size: 970 1380 1875 1156 2573
Fragments arranged by size:
2573 1875 1380 1156 970
BmgBI CAC'GTC
Cuts at: 1318 2779 1318
Size: 1461 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2411 4011 4936
Size: 416 53 83 399 24 994 1600 925
Cuts at: 4936 5685 6643 442
Size: 749 958 1753
Fragments arranged by size:
1753 1600 994 958 925 749 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4818 1056
Size: 3762 4192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 4219 4933 2329
Size: 1890 714 5350
Fragments arranged by size:
5350 1890 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2398 3227 4044 4355 4878 4903 5102 6614
Size: 1159 829 817 311 523 25 199 1512
Cuts at: 6614 1239
Size: 2579
Fragments arranged by size:
2579 1512 1159 829 817 523 311 199
25
Bpu10I CC'TnA_GC
Cuts at: 2865 3314 4488 4900 2865
Size: 449 1174 412 5919
Fragments arranged by size:
5919 1174 449 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 3347 5195 6079 6944 7242 2819
Size: 528 1848 884 865 298 3531
Fragments arranged by size:
3531 1848 884 865 528 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3653 6617 882
Size: 617 2154 2964 2219
Fragments arranged by size:
2964 2219 2154 617
BsaAI yAC'GTr
Cuts at: 493 1204 2671 2695 2878 3424 5745 493
Size: 711 1467 24 183 546 2321 2702
Fragments arranged by size:
2702 2321 1467 711 546 183 24
BsaBI GATnn'nnATC
Cuts at: 2343 2523 3338 7948 2343
Size: 180 815 4610 2349
Fragments arranged by size:
4610 2349 815 180
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4261 4295 4316 4409
Size: 53 83 186 936 2728 34 21 93
Cuts at: 4409 4625 4634 4736 5151 5761 6143 6778 275
Size: 216 9 102 415 610 382 635 1451
Fragments arranged by size:
2728 1451 936 635 610 415 382 216
186 102 93 83 53 34 21 9
BsaWI w'CCGG_w
Cuts at: 6387 6785 7059 7206 7617 6387
Size: 398 274 147 411 6724
Fragments arranged by size:
6724 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2436 2466 2885 2915 1178
Size: 30 165 30 1033 30 419 30 6217
Fragments arranged by size:
6217 1033 419 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2084 2156 2159 2168 2171 2192
Size: 865 3 30 72 3 9 3 21
Cuts at: 2192 3494 1186
Size: 1302 5646
Fragments arranged by size:
5646 1302 865 72 30 21 9 3
3 3
BseYI C'CCAG_C
Cuts at: 2490 2885 3382 3929 4703 4709 4744 4771 4820
Size: 395 497 547 774 6 35 27 49
Cuts at: 4820 7157 7653 2490
Size: 2337 496 2791
Fragments arranged by size:
2791 2337 774 547 497 496 395 49
35 27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 3632 4184 1163
Size: 573 385 1511 552 4933
Fragments arranged by size:
4933 1511 573 552 385
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 5404 6165 7193 1540
Size: 275 186 934 2469 761 1028 2301
Fragments arranged by size:
2469 2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2475 2784 3227 3246 3291 3534 4864 5030 5079
Size: 309 443 19 45 243 1330 166 49
Cuts at: 5079 6015 6100 7171 2475
Size: 936 85 1071 3258
Fragments arranged by size:
3258 1330 1071 936 443 309 243 166
85 49 45 19
BsmI GAATG_Cn'
Cuts at: 5465 5558 5465
Size: 93 7861
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 7954
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 2475 2760 2784 2886 3227 3246
Size: 399 569 116 285 24 102 341 19
Cuts at: 3246 3291 3534 3938 4140 4256 4524 4832 4864
Size: 45 243 404 202 116 268 308 32
Cuts at: 4864 5030 5079 5273 6015 6100 7171 1391
Size: 166 49 194 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 404 399 341 308
285 268 243 202 194 166 116 116
102 85 49 45 32 24 19
BspEI T'CCGG_A
Cuts at: 7617 7617
Size: 7954
BspHI T'CATG_A
Cuts at: 5843 7573 5843
Size: 1730 6224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2733 3306 3552 4217 5309 844
Size: 1889 573 246 665 1092 3489
Fragments arranged by size:
3489 1889 1092 665 573 246
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 3238 5841 1394
Size: 429 269 1146 2603 3507
Fragments arranged by size:
3507 2603 1146 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 2592 2859 3865 6449 6623 65
Size: 1183 1344 267 1006 2584 174 1396
Fragments arranged by size:
2584 1396 1344 1183 1006 267 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 6598 6785 1700
Size: 110 2826 1962 187 2869
Fragments arranged by size:
2869 2826 1962 187 110
BsrGI T'GTAC_A
Cuts at: 96 4644 96
Size: 4548 3406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7954
BssSI C'ACGA_G
Cuts at: 6014 7026 6014
Size: 1012 6942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2500 2755 794
Size: 1706 255 5993
Fragments arranged by size:
5993 1706 255
BstBI TT'CG_AA
Cuts at: 6758 6758
Size: 7954
BstEII G'GTnAC_C
Cuts at: 1184 4930 1184
Size: 3746 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 2823 4225 4571 2823
Size: 1402 346 6206
Fragments arranged by size:
6206 1402 346
BstYI r'GATC_y
Cuts at: 1972 2462 2518 3309 3619 5214 5284 5651 6036
Size: 490 56 791 310 1595 70 367 385
Cuts at: 6036 6053 7505 7591 7603 7949 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1595 1452 791 490 385 367 346
310 86 70 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2130 2130
Size: 7954
BtgI C'CryG_G
Cuts at: 513 1065 2218 2565 3966 4182 4245 4800 5145
Size: 552 1153 347 1401 216 63 555 345
Cuts at: 5145 513
Size: 3322
Fragments arranged by size:
3322 1401 1153 555 552 347 345 216
63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7215
Fragments arranged by size:
7215 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2116 4069 5014 5470 6264 6284 2065
Size: 51 1953 945 456 794 20 3735
Fragments arranged by size:
3735 1953 945 794 456 51 20
ClaI AT'CG_AT
Cuts at: 1554 2322 1554
Size: 768 7186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7919
DraI TTT'AAA
Cuts at: 5258 5258
Size: 7954
DraIII CAC_nnn'GTG
Cuts at: 3922 4732 3922
Size: 810 7144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6961 809
Size: 507 5645 1802
Fragments arranged by size:
5645 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 2802 3439
Size: 54 1425 50 275 18 738 234 637
Cuts at: 3439 4111 4638 5401 6290 7919 8
Size: 672 527 763 889 1629 43
Fragments arranged by size:
1629 1425 889 763 738 672 637 527
275 234 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5401 1537
Size: 275 3589 4090
Fragments arranged by size:
4090 3589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2118 2133 2145 2169 3848 5884 1353
Size: 729 36 15 12 24 1679 2036 3423
Fragments arranged by size:
3423 2036 1679 729 36 24 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 2819 6541 6915 7061 387
Size: 1064 138 521 709 3722 374 146 1280
Fragments arranged by size:
3722 1280 1064 709 521 374 146 138
EcoICRI GAG'CTC
Cuts at: 2473 3289 4862 2473
Size: 816 1573 5565
Fragments arranged by size:
5565 1573 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2398 2927 3227 3545 4044 4355 4878
Size: 393 766 529 300 318 499 311 523
Cuts at: 4878 4903 5060 5102 6017 6614 7401 7669 1239
Size: 25 157 42 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 766 597 529 523 499
393 318 311 300 268 157 42 25
EcoNI CCTnn'n_nnAGG
Cuts at: 4288 5129 4288
Size: 841 7113
EcoO109I rG'GnC_Cy
Cuts at: 3030 3358 3541 4136 4137 4449 4456 3030
Size: 328 183 595 1 312 7 6528
Fragments arranged by size:
6528 595 328 312 183 7 1
EcoRI G'AATT_C
Cuts at: 5263 5641 5263
Size: 378 7576
FspI TGC'GCA
Cuts at: 2585 4124 6460 2585
Size: 1539 2336 4079
Fragments arranged by size:
4079 2336 1539
FspAI rTGC'GCAy
Cuts at: 4124 4124
Size: 7954
HaeII r_GCGC'y
Cuts at: 1678 4264 4739 7101 1678
Size: 2586 475 2362 2531
Fragments arranged by size:
2586 2531 2362 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 3035 3067 3551
Size: 32 135 32 887 32 544 32 484
Cuts at: 3551 3583 3823 3855 4574 4606 4646 4678 6595
Size: 32 240 32 719 32 40 32 1917
Cuts at: 6595 6627 6669 6701 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1917 887 719 544 484 240 135
42 40 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2251 4603 5298 5544 669
Size: 988 281 313 2352 695 246 3079
Fragments arranged by size:
3079 2352 988 695 313 281 246
HindIII A'AGCT_T
Cuts at: 748 3850 748
Size: 3102 4852
HpaI GTT'AAC
Cuts at: 5544 5544
Size: 7954
KasI G'GCGC_C
Cuts at: 4260 4735 4260
Size: 475 7479
KpnI G_GTAC'C
Cuts at: 5279 5279
Size: 7954
MfeI C'AATT_G
Cuts at: 5553 5553
Size: 7954
MluI A'CGCG_T
Cuts at: 7830 7830
Size: 7954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 3277 3354 7068 7252 1305
Size: 684 1288 77 3714 184 2007
Fragments arranged by size:
3714 2007 1288 684 184 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 5160
Fragments arranged by size:
5160 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 3341 3776 4017 4157 4223 4247
Size: 648 1503 672 435 241 140 66 24
Cuts at: 4247 4946 5912 6271 6430 518
Size: 699 966 359 159 2042
Fragments arranged by size:
2042 1503 966 699 672 648 435 359
241 159 140 66 24
MspA1I CmG'CkG
Cuts at: 1606 2105 2500 3616 4237 5370 6047 7195 7440
Size: 499 395 1116 621 1133 677 1148 245
Cuts at: 7440 1606
Size: 2120
Fragments arranged by size:
2120 1148 1133 1116 677 621 499 395
245
NaeI GCC'GGC
Cuts at: 4638 4638
Size: 7954
NarI GG'CG_CC
Cuts at: 4261 4736 4261
Size: 475 7479
NcoI C'CATG_G
Cuts at: 513 1065 2218 4800 513
Size: 552 1153 2582 3667
Fragments arranged by size:
3667 2582 1153 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7954
NgoMIV G'CCGG_C
Cuts at: 4636 4636
Size: 7954
NheI G'CTAG_C
Cuts at: 1052 4814 1052
Size: 3762 4192
NotI GC'GGCC_GC
Cuts at: 5401 5401
Size: 7954
NsiI A_TGCA'T
Cuts at: 3519 3519
Size: 7954
NspI r_CATG'y
Cuts at: 1132 1229 2654 3373 3682 3898 5026 5312 6857
Size: 97 1425 719 309 216 1128 286 1545
Cuts at: 6857 1132
Size: 2229
Fragments arranged by size:
2229 1545 1425 1128 719 309 286 216
97
PasI CC'CwG_GG
Cuts at: 1348 3034 4132 1348
Size: 1686 1098 5170
Fragments arranged by size:
5170 1686 1098
PciI A'CATG_T
Cuts at: 3369 3678 6853 3369
Size: 309 3175 4470
Fragments arranged by size:
4470 3175 309
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2529 2752 3631 4732 4926 1108
Size: 1421 223 879 1101 194 4136
Fragments arranged by size:
4136 1421 1101 879 223 194
PfoI T'CCnGG_A
Cuts at: 2334 4371 5244 2334
Size: 2037 873 5044
Fragments arranged by size:
5044 2037 873
PmeI GTTT'AAAC
Cuts at: 5258 5258
Size: 7954
PmlI CAC'GTG
Cuts at: 2695 3424 2695
Size: 729 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 6023 6055 7536 7568 2885
Size: 32 3106 32 1481 32 3271
Fragments arranged by size:
3271 3106 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3030 3358 3541 4456 3030
Size: 328 183 915 6528
Fragments arranged by size:
6528 915 328 183
PshAI GACnn'nnGTC
Cuts at: 1613 4997 1613
Size: 3384 4570
PsiI TTA'TAA
Cuts at: 5524 5524
Size: 7954
PspOMI G'GGCC_C
Cuts at: 4136 4136
Size: 7954
PstI C_TGCA'G
Cuts at: 830 2625 3303 3549 5306 830
Size: 1795 678 246 1757 3478
Fragments arranged by size:
3478 1795 1757 678 246
PvuI CG_AT'CG
Cuts at: 2001 2935 2001
Size: 934 7020
PvuII CAG'CTG
Cuts at: 2500 3616 2500
Size: 1116 6838
SacI G_AGCT'C
Cuts at: 2475 3291 4864 2475
Size: 816 1573 5565
Fragments arranged by size:
5565 1573 816
SalI G'TCGA_C
Cuts at: 1936 5296 1936
Size: 3360 4594
SanDI GG'GwC_CC
Cuts at: 4456 4456
Size: 7954
SapI GCTCTTCn'nnn_
Cuts at: 2082 2118 2082
Size: 36 7918
SbfI CC_TGCA'GG
Cuts at: 3303 5306 3303
Size: 2003 5951
ScaI AGT'ACT
Cuts at: 6202 7914 6202
Size: 1712 6242
SexAI A'CCwGG_T
Cuts at: 3918 4728 3918
Size: 810 7144
SfcI C'TryA_G
Cuts at: 826 1045 2621 3299 3545 4840 5302 6437 7118
Size: 219 1576 678 246 1295 462 1135 681
Cuts at: 7118 7309 826
Size: 191 1471
Fragments arranged by size:
1576 1471 1295 1135 681 678 462 246
219 191
SfoI GGC'GCC
Cuts at: 4262 4737 4262
Size: 475 7479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7954
SmaI CCC'GGG
Cuts at: 1872 3539 4266 5281 1872
Size: 1667 727 1015 4545
Fragments arranged by size:
4545 1667 1015 727
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2834 3362 5210 6058 6959 7221
Size: 197 926 891 528 1848 848 901 262
Cuts at: 7221 820
Size: 1553
Fragments arranged by size:
1848 1553 926 901 891 848 528 262
197
SnaBI TAC'GTA
Cuts at: 493 5745 493
Size: 5252 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7954
SphI G_CATG'C
Cuts at: 1132 5312 1132
Size: 4180 3774
SrfI GCCC'GGGC
Cuts at: 4266 4266
Size: 7954
SspI AAT'ATT
Cuts at: 5 52 5878 5
Size: 47 5826 2081
Fragments arranged by size:
5826 2081 47
StuI AGG'CCT
Cuts at: 1517 2678 3462 4541 1517
Size: 1161 784 1079 4930
Fragments arranged by size:
4930 1161 1079 784
StyI C'CwwG_G
Cuts at: 513 1065 2218 3750 4800 5393 513
Size: 552 1153 1532 1050 593 3074
Fragments arranged by size:
3074 1532 1153 1050 593 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2211 2504 3623 3959 4360 5981 5998
Size: 1255 631 293 1119 336 401 1621 17
Cuts at: 5998 6151 6336 6781 325
Size: 153 185 445 1498
Fragments arranged by size:
1621 1498 1255 1119 631 445 401 336
293 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4644 6200 7912
Size: 274 80 33 51 1443 2667 1556 1712
Cuts at: 7912 96
Size: 138
Fragments arranged by size:
2667 1712 1556 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 7914 7946 2436
Size: 32 5446 32 2444
Fragments arranged by size:
5446 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7954
XbaI T'CTAG_A
Cuts at: 2302 5290 2302
Size: 2988 4966
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2282 2437 2713 3964 4189 5077 2282
Size: 155 276 1251 225 888 5159
Fragments arranged by size:
5159 1251 888 276 225 155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7954
XmaI C'CCGG_G
Cuts at: 1870 3537 4264 5279 1870
Size: 1667 727 1015 4545
Fragments arranged by size:
4545 1667 1015 727
XmnI GAAnn'nnTTC
Cuts at: 6083 6083
Size: 7954
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5762 276
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BstZ17I EcoRV FalI FseI
NruI PacI PspXI PsrI RsrII SacII SfiI SwaI