Restriction map for
pFN21AB4091
(Circular) (Six-base) MAPSORT of: pFN21AB4091.seq Check: 745 from: 1 to: 6145
pFN21AB4091 6145 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3267 3488 1937
Size: 1330 221 4594
Fragments arranged by size:
4594 1330 221
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 3010 4208 5592 5860 1632
Size: 576 802 1198 1384 268 1917
Fragments arranged by size:
1917 1384 1198 802 576 268
AfeI AGC'GCT
Cuts at: 2012 2012
Size: 6145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 3045 5044 6021 1203
Size: 1842 1999 977 1327
Fragments arranged by size:
1999 1842 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2251 1184
Size: 1067 5078
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 2447 2659 3310 3454 3680 3832 5941 1493
Size: 954 212 651 144 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 651 226 212 152 144
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 3470 1870
Size: 73 385 1142 4545
Fragments arranged by size:
4545 1142 385 73
BamHI G'GATC_C
Cuts at: 3475 3842 3475
Size: 367 5778
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2795 3466 4921 618
Size: 325 217 627 1008 671 1455 1842
Fragments arranged by size:
1842 1455 1008 671 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 2551 3464 1391
Size: 652 508 913 4072
Fragments arranged by size:
4072 913 652 508
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2811 2917 928
Size: 971 912 106 4156
Fragments arranged by size:
4156 971 912 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2898 2932 4336 4370 1903
Size: 34 961 34 1404 34 3678
Fragments arranged by size:
3678 1404 961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2290 2583 4037 5247 2290
Size: 293 1454 1210 3188
Fragments arranged by size:
3188 1454 1210 293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2410 6140 2410
Size: 3730 2415
BlpI GC'TnA_GC
Cuts at: 2042 3562 2042
Size: 1520 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2441 4206 5362 1790
Size: 651 1765 1156 2573
Fragments arranged by size:
2573 1765 1156 651
BmgBI CAC'GTC
Cuts at: 1318 2588 1318
Size: 1270 4875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2498 3876
Size: 416 53 83 399 24 636 445 1378
Cuts at: 3876 4834 442
Size: 958 1753
Fragments arranged by size:
1753 1378 958 636 445 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3001 4805 1239
Size: 1762 1804 2579
Fragments arranged by size:
2579 1804 1762
Bpu10I CC'TnA_GC
Cuts at: 2479 2479
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 2088 6139 2088
Size: 4051 2094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3952 4334 4969 275
Size: 53 83 186 936 2419 382 635 1451
Fragments arranged by size:
2419 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5920
Fragments arranged by size:
5920 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3452 1186
Size: 2266 3879
BseYI C'CCAG_C
Cuts at: 3424 5348 5844 3424
Size: 1924 496 3725
Fragments arranged by size:
3725 1924 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2221 3415 1163
Size: 573 485 1194 3893
Fragments arranged by size:
3893 1194 573 485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 4206 4291 5362 2551
Size: 1655 85 1071 3334
Fragments arranged by size:
3334 1655 1071 85
BsmI GAATG_Cn'
Cuts at: 2375 3103 3656 3749 2375
Size: 728 553 93 4771
Fragments arranged by size:
4771 728 553 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2441 2551 3464 4206 4291 5362
Size: 399 253 398 110 913 742 85 1071
Cuts at: 5362 1391
Size: 2174
Fragments arranged by size:
2174 1071 913 742 399 398 253 110
85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 3500 844
Size: 1375 1281 3489
Fragments arranged by size:
3489 1375 1281
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2784 2914 3250 3407 4640 4814 65
Size: 1183 1536 130 336 157 1233 174 1396
Fragments arranged by size:
1536 1396 1233 1183 336 174 157 130
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2405 2781 3208 96
Size: 2309 376 427 3033
Fragments arranged by size:
3033 2309 427 376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2410 3145 3475 3842 4227 4244 5696 5782
Size: 438 735 330 367 385 17 1452 86
Cuts at: 5782 5794 6140 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 735 438 385 367 346 330
86 17 12
BstZ17I GTA'TAC
Cuts at: 3268 3268
Size: 6145
BtgI C'CryG_G
Cuts at: 513 1065 2645 513
Size: 552 1580 4013
Fragments arranged by size:
4013 1580 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 3009 3661 4455 4475 3009
Size: 652 794 20 4679
Fragments arranged by size:
4679 794 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2377 2412 529
Size: 35 1813 35 4262
Fragments arranged by size:
4262 1813 35 35
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2642 3112 3592
Size: 54 1425 50 275 18 812 470 480
Cuts at: 3592 4481 6110 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 812 480 470 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4075 1353
Size: 2722 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2549 2549
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 3001 3010 4208 4805 5592 5860
Size: 393 576 793 9 1198 597 787 268
Cuts at: 5860 1239
Size: 1524
Fragments arranged by size:
1524 1198 793 787 597 576 393 268
9
EcoO109I rG'GnC_Cy
Cuts at: 2231 2231
Size: 6145
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2014 5292 1678
Size: 336 3278 2531
Fragments arranged by size:
3278 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2335 2367 4786
Size: 32 135 32 614 32 117 32 2419
Cuts at: 4786 4818 4860 4892 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2419 614 135 117 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3182 5259 5443 1305
Size: 1877 2077 184 2007
Fragments arranged by size:
2077 2007 1877 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3114 10
Size: 54 1425 343 1282 3041
Fragments arranged by size:
3041 1425 1282 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3044 4103 4462 4621 518
Size: 648 1878 1059 359 159 2042
Fragments arranged by size:
2042 1878 1059 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2624 3561 4238 5386 5631 1606
Size: 1018 937 677 1148 245 2120
Fragments arranged by size:
2120 1148 1018 937 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 2845 387
Size: 2458 3687
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3049 3503 5048 1132
Size: 97 1820 454 1545 2229
Fragments arranged by size:
2229 1820 1545 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 3045 5044 3045
Size: 1999 4146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2231 2231
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 2609 3715 2609
Size: 1106 5039
PstI C_TGCA'G
Cuts at: 830 2629 3497 830
Size: 1799 868 3478
Fragments arranged by size:
3478 1799 868
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2624 2624
Size: 6145
SacI G_AGCT'C
Cuts at: 2551 2551
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2625 2752 3493 4628 5309 5500 826
Size: 219 1580 127 741 1135 681 191 1471
Fragments arranged by size:
1580 1471 1135 741 681 219 191 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2698 3062 3383 3584 513
Size: 552 1633 364 321 201 3074
Fragments arranged by size:
3074 1633 552 364 321 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4172 4189 4342 4527 4972 325
Size: 1255 2592 17 153 185 445 1498
Fragments arranged by size:
2592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2405 2781 3208
Size: 274 80 33 51 1443 428 376 427
Cuts at: 3208 4391 6103 96
Size: 1183 1712 138
Fragments arranged by size:
1712 1443 1183 428 427 376 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2230 2262 6105 6137 2230
Size: 32 3843 32 2238
Fragments arranged by size:
3843 2238 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2806 2806
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI