Restriction map for
pFN21AB4085
(Circular) (Six-base) MAPSORT of: pFN21AB4085.seq Check: 9241 from: 1 to: 7624
pFN21AB4085 7624 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3745 3745
Size: 7624
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4082 4136 5434 278
Size: 53 83 186 936 2546 54 1298 2468
Fragments arranged by size:
2546 2468 1298 936 186 83 54 53
AccI GT'mk_AC
Cuts at: 1937 2205 4967 1937
Size: 268 2762 4594
Fragments arranged by size:
4594 2762 268
Acc65I G'GTAC_C
Cuts at: 2518 3197 3841 4945 2518
Size: 679 644 1104 5197
Fragments arranged by size:
5197 1104 679 644
AclI AA'CG_TT
Cuts at: 1563 5751 6124 1563
Size: 4188 373 3063
Fragments arranged by size:
4188 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3165 4399 4412 5687 7071 7339 1632
Size: 1533 1234 13 1275 1384 268 1917
Fragments arranged by size:
1917 1533 1384 1275 1234 268 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7427
AflIII A'CryG_T
Cuts at: 1203 3621 4191 6523 7500 1203
Size: 2418 570 2332 977 1327
Fragments arranged by size:
2418 2332 1327 977 570
AgeI A'CCGG_T
Cuts at: 6455 6455
Size: 7624
AhdI GACnn_n'nnGTC
Cuts at: 6353 6353
Size: 7624
AleI CACnn'nnGTG
Cuts at: 1166 2231 2879 4316 1166
Size: 1065 648 1437 4474
Fragments arranged by size:
4474 1437 1065 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3201 1184
Size: 2017 5607
AlwNI CAG_nnn'CTG
Cuts at: 2140 2254 2774 5367 6939 2140
Size: 114 520 2593 1572 2825
Fragments arranged by size:
2825 2593 1572 520 114
ApaLI G'TGCA_C
Cuts at: 3629 4532 5681 6837 3629
Size: 903 1149 1156 4416
Fragments arranged by size:
4416 1156 1149 903
ApoI r'AATT_y
Cuts at: 1493 2359 2629 2928 3009 3051 4733 4933 5159
Size: 866 270 299 81 42 1682 200 226
Cuts at: 5159 5311 7420 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1682 866 299 270 226 200
152 81 42
AseI AT'TA_AT
Cuts at: 160 1032 6178 160
Size: 872 5146 1606
Fragments arranged by size:
5146 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 3473 4437 4949 1870
Size: 73 182 1348 964 512 4545
Fragments arranged by size:
4545 1348 964 512 182 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3833 3866 3833
Size: 33 7591
BamHI G'GATC_C
Cuts at: 2129 4162 4954 5321 2129
Size: 2033 792 367 4432
Fragments arranged by size:
4432 2033 792 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2278 2518 3197 3841
Size: 325 217 627 438 53 240 679 644
Cuts at: 3841 4945 6400 618
Size: 1104 1455 1842
Fragments arranged by size:
1842 1455 1104 679 644 627 438 325
240 217 53
BanII G_rGCy'C
Cuts at: 1391 3395 4071 4391 4943 1391
Size: 2004 676 320 552 4072
Fragments arranged by size:
4072 2004 676 552 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5815 5849 1903
Size: 34 3878 34 3678
Fragments arranged by size:
3878 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2420 4609 5516 6726 2420
Size: 2189 907 1210 3318
Fragments arranged by size:
3318 2189 1210 907
BclI T'GATC_A
Cuts at: 1548 4579 1548
Size: 3031 4593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6235 136
Size: 107 122 71 631 605 4563 1525
Fragments arranged by size:
4563 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7619 7619
Size: 7624
BlpI GC'TnA_GC
Cuts at: 4270 5041 4270
Size: 771 6853
Bme1580I G_kGCm'C
Cuts at: 1790 3633 4536 5685 6841 1790
Size: 1843 903 1149 1156 2573
Fragments arranged by size:
2573 1843 1156 1149 903
BmgBI CAC'GTC
Cuts at: 1318 2461 3127 3442 1318
Size: 1143 666 315 5500
Fragments arranged by size:
5500 1143 666 315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4455 5355 6313
Size: 416 53 83 399 24 3038 900 958
Cuts at: 6313 442
Size: 1753
Fragments arranged by size:
3038 1753 958 900 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2345 2438 3258 3704 4309 4626 6284
Size: 1043 63 93 820 446 605 317 1658
Cuts at: 6284 1239
Size: 2579
Fragments arranged by size:
2579 1658 1043 820 605 446 317 93
63
Bpu10I CC'TnA_GC
Cuts at: 2247 3189 2247
Size: 942 6682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5749 6614 6912 5749
Size: 865 298 6461
Fragments arranged by size:
6461 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 6287 882
Size: 617 3330 1458 2219
Fragments arranged by size:
3330 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 3622 3868 3970 5415 493
Size: 711 2418 246 102 1445 2702
Fragments arranged by size:
2702 2418 1445 711 246 102
BsaBI GATnn'nnATC
Cuts at: 2741 7618 2741
Size: 4877 2747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 3717 4079 4133
Size: 53 83 186 936 767 1417 362 54
Cuts at: 4133 5431 5813 6448 275
Size: 1298 382 635 1451
Fragments arranged by size:
1451 1417 1298 936 767 635 382 362
186 83 54 53
BsaWI w'CCGG_w
Cuts at: 4709 6057 6455 6729 6876 7287 4709
Size: 1348 398 274 147 411 5046
Fragments arranged by size:
5046 1348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3153 3183 3813 3843 3884
Size: 30 165 30 1750 30 630 30 41
Cuts at: 3884 3914 4487 4517 1178
Size: 30 573 30 4285
Fragments arranged by size:
4285 1750 630 573 165 41 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 3692 4375 4556 4847 1186
Size: 1039 1467 683 181 291 3963
Fragments arranged by size:
3963 1467 1039 683 291 181
BseYI C'CCAG_C
Cuts at: 2133 2834 6827 7323 2133
Size: 701 3993 496 2434
Fragments arranged by size:
3993 2434 701 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2425 1163
Size: 573 689 6362
Fragments arranged by size:
6362 689 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2374 3851 3948 5074 5835 6863
Size: 275 186 373 1477 97 1126 761 1028
Cuts at: 6863 1540
Size: 2301
Fragments arranged by size:
2301 1477 1126 1028 761 373 275 186
97
BsiHKAI G_wGCw'C
Cuts at: 3606 3633 4239 4325 4379 4536 5685 5770 6841
Size: 27 606 86 54 157 1149 85 1071
Cuts at: 6841 3606
Size: 4389
Fragments arranged by size:
4389 1149 1071 606 157 86 85 54
27
BsmI GAATG_Cn'
Cuts at: 4551 5135 5228 4551
Size: 584 93 6947
Fragments arranged by size:
6947 584 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3395 3606 3633 4071 4239 4325 4379
Size: 399 1605 211 27 438 168 86 54
Cuts at: 4379 4391 4536 4943 5685 5770 6841 1391
Size: 12 145 407 742 85 1071 2174
Fragments arranged by size:
2174 1605 1071 742 438 407 399 211
168 145 86 85 54 27 12
BspEI T'CCGG_A
Cuts at: 7287 7287
Size: 7624
BspHI T'CATG_A
Cuts at: 5513 7243 5513
Size: 1730 5894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 3083 3745 4979 844
Size: 1590 649 662 1234 3489
Fragments arranged by size:
3489 1590 1234 662 649
BsrBI CCG'CTC
Cuts at: 1394 1823 2184 3589 3642 5511 1394
Size: 429 361 1405 53 1869 3507
Fragments arranged by size:
3507 1869 1405 429 361 53
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 3204 6119 6293 65
Size: 1183 858 1098 2915 174 1396
Fragments arranged by size:
2915 1396 1183 1098 858 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2491 3719 3787 3937 4290 6268 6455
Size: 110 681 1228 68 150 353 1978 187
Cuts at: 6455 1700
Size: 2869
Fragments arranged by size:
2869 1978 1228 681 353 187 150 110
68
BsrGI T'GTAC_A
Cuts at: 96 3978 4251 96
Size: 3882 273 3469
Fragments arranged by size:
3882 3469 273
BssHII G'CGCG_C
Cuts at: 1925 3708 3710 3883 3951 4296 1925
Size: 1783 2 173 68 345 5253
Fragments arranged by size:
5253 1783 345 173 68 2
BssSI C'ACGA_G
Cuts at: 3674 5684 6696 3674
Size: 2010 1012 4602
Fragments arranged by size:
4602 2010 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7624
BstBI TT'CG_AA
Cuts at: 6428 6428
Size: 7624
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7624
BstXI CCAn_nnnn'nTGG
Cuts at: 4096 4231 4333 4096
Size: 135 102 7387
Fragments arranged by size:
7387 135 102
BstYI r'GATC_y
Cuts at: 1972 2129 4162 4748 4954 5321 5706 5723 7175
Size: 157 2033 586 206 367 385 17 1452
Cuts at: 7175 7261 7273 7619 1972
Size: 86 12 346 1977
Fragments arranged by size:
2033 1977 1452 586 385 367 346 206
157 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2043
Size: 7624
BtgI C'CryG_G
Cuts at: 513 1065 3087 3411 3426 3845 4060 4083 4089
Size: 552 2022 324 15 419 215 23 6
Cuts at: 4089 4332 513
Size: 243 3805
Fragments arranged by size:
3805 2022 552 419 324 243 215 23
15 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2227 4189 487
Size: 683 56 1001 1962 3922
Fragments arranged by size:
3922 1962 1001 683 56
BtsI GCAGTG_nn'
Cuts at: 3791 4254 4592 5140 5934 5954 3791
Size: 463 338 548 794 20 5461
Fragments arranged by size:
5461 794 548 463 338 20
ClaI AT'CG_AT
Cuts at: 1554 2745 1554
Size: 1191 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7589
DraI TTT'AAA
Cuts at: 4452 4681 4928 4452
Size: 229 247 7148
Fragments arranged by size:
7148 247 229
DraIII CAC_nnn'GTG
Cuts at: 3871 3871
Size: 7624
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6631 809
Size: 507 5315 1802
Fragments arranged by size:
5315 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2185 3435 4086
Size: 54 1425 50 275 18 355 1250 651
Cuts at: 4086 4117 4589 5071 5960 7589 8
Size: 31 472 482 889 1629 43
Fragments arranged by size:
1629 1425 1250 889 651 482 472 355
275 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5071 1537
Size: 275 3259 4090
Fragments arranged by size:
4090 3259 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3027 5554 1353
Size: 1674 2527 3423
Fragments arranged by size:
3423 2527 1674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 2407 4034 6211 6585 6731
Size: 1064 138 696 122 1627 2177 374 146
Cuts at: 6731 387
Size: 1280
Fragments arranged by size:
2177 1627 1280 1064 696 374 146 138
122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2345 2438 3165 3258 3704 4309
Size: 393 650 63 93 727 93 446 605
Cuts at: 4309 4399 4412 4626 5687 6284 7071 7339 1239
Size: 90 13 214 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 727 650 605 597 446
393 268 214 93 93 90 63 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 4013 4541 2324
Size: 1689 528 5407
Fragments arranged by size:
5407 1689 528
EcoO109I rG'GnC_Cy
Cuts at: 2085 2251 2317 4123 4574 2085
Size: 166 66 1806 451 5135
Fragments arranged by size:
5135 1806 451 166 66
EcoRI G'AATT_C
Cuts at: 2359 4933 5311 2359
Size: 2574 378 4672
Fragments arranged by size:
4672 2574 378
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 7624
FspI TGC'GCA
Cuts at: 6130 6130
Size: 7624
HaeII r_GCGC'y
Cuts at: 1678 2571 3336 4158 4277 4332 6771 1678
Size: 893 765 822 119 55 2439 2531
Fragments arranged by size:
2531 2439 893 822 765 119 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3218 3240 3250 3272 3338
Size: 32 135 32 1646 22 10 22 66
Cuts at: 3338 3370 3377 3409 3884 3916 6265 6297 6339
Size: 32 7 32 475 32 2349 32 42
Cuts at: 6339 6371 1373
Size: 32 2626
Fragments arranged by size:
2626 2349 1646 475 135 66 42 32
32 32 32 32 32 32 22 22
10 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3061 4660 4968 5214 669
Size: 988 281 1123 1599 308 246 3079
Fragments arranged by size:
3079 1599 1123 988 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7624
HpaI GTT'AAC
Cuts at: 3061 5214 3061
Size: 2153 5471
KpnI G_GTAC'C
Cuts at: 2522 3201 3845 4949 2522
Size: 679 644 1104 5197
Fragments arranged by size:
5197 1104 679 644
MfeI C'AATT_G
Cuts at: 5223 5223
Size: 7624
MluI A'CGCG_T
Cuts at: 7500 7500
Size: 7624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2911 3204 6738 6922 1305
Size: 1606 293 3534 184 2007
Fragments arranged by size:
3534 2007 1606 293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4591 10
Size: 54 1425 343 2759 3043
Fragments arranged by size:
3043 2759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2879 4316 5582 5941 6100 518
Size: 648 1065 648 1437 1266 359 159 2042
Fragments arranged by size:
2042 1437 1266 1065 648 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 3847 4432 4671 5040 5717 6865 7110
Size: 834 1407 585 239 369 677 1148 245
Cuts at: 7110 1606
Size: 2120
Fragments arranged by size:
2120 1407 1148 834 677 585 369 245
239
NaeI GCC'GGC
Cuts at: 3721 3789 3721
Size: 68 7556
NcoI C'CATG_G
Cuts at: 513 1065 3087 513
Size: 552 2022 5050
Fragments arranged by size:
5050 2022 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7624
NgoMIV G'CCGG_C
Cuts at: 3719 3787 3719
Size: 68 7556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7624
NotI GC'GGCC_GC
Cuts at: 5071 5071
Size: 7624
NspI r_CATG'y
Cuts at: 1132 1229 4522 4982 6527 1132
Size: 97 3293 460 1545 2229
Fragments arranged by size:
3293 2229 1545 460 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7624
PciI A'CATG_T
Cuts at: 6523 6523
Size: 7624
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3871 1108
Size: 2763 4861
PfoI T'CCnGG_A
Cuts at: 2796 4008 4567 2796
Size: 1212 559 5853
Fragments arranged by size:
5853 1212 559
PmeI GTTT'AAAC
Cuts at: 4928 4928
Size: 7624
PmlI CAC'GTG
Cuts at: 3868 3970 3868
Size: 102 7522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5693 5725 7206 7238 5693
Size: 32 1481 32 6079
Fragments arranged by size:
6079 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2317 2251
Size: 66 7558
PshAI GACnn'nnGTC
Cuts at: 1613 2814 1613
Size: 1201 6423
PsiI TTA'TAA
Cuts at: 5194 5194
Size: 7624
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2502 2534 3470 3502 2502
Size: 32 936 32 6624
Fragments arranged by size:
6624 936 32 32
PstI C_TGCA'G
Cuts at: 830 2340 2445 3663 3696 3756 4384 4976 830
Size: 1510 105 1218 33 60 628 592 3478
Fragments arranged by size:
3478 1510 1218 628 592 105 60 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7624
PvuII CAG'CTG
Cuts at: 4432 4671 4432
Size: 239 7385
SacII CC_GC'GG
Cuts at: 3848 3848
Size: 7624
SalI G'TCGA_C
Cuts at: 1936 4966 1936
Size: 3030 4594
SbfI CC_TGCA'GG
Cuts at: 4976 4976
Size: 7624
ScaI AGT'ACT
Cuts at: 5872 7584 5872
Size: 1712 5912
SexAI A'CCwGG_T
Cuts at: 4920 4920
Size: 7624
SfcI C'TryA_G
Cuts at: 826 1045 2336 2441 3659 3692 3752 4380 4972
Size: 219 1291 105 1218 33 60 628 592
Cuts at: 4972 6107 6788 6979 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1291 1218 1135 681 628 592 219
191 105 60 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7624
SgrAI Cr'CCGG_yG
Cuts at: 1700 2491 3719 1700
Size: 791 1228 5605
Fragments arranged by size:
5605 1228 791
SmaI CCC'GGG
Cuts at: 1872 2127 4951 1872
Size: 255 2824 4545
Fragments arranged by size:
4545 2824 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4437 5728 6629 6891 820
Size: 197 926 2494 1291 901 262 1553
Fragments arranged by size:
2494 1553 1291 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5415 493
Size: 4922 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7624
SphI G_CATG'C
Cuts at: 1132 4522 4982 1132
Size: 3390 460 3774
Fragments arranged by size:
3774 3390 460
SspI AAT'ATT
Cuts at: 5 52 5548 5
Size: 47 5496 2081
Fragments arranged by size:
5496 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7624
StyI C'CwwG_G
Cuts at: 513 1065 3087 5063 513
Size: 552 2022 1976 3074
Fragments arranged by size:
3074 2022 1976 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 5651 5668 5821 6006 6451 325
Size: 1255 2265 1806 17 153 185 445 1498
Fragments arranged by size:
2265 1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3477 3978
Size: 274 80 33 51 1443 30 1470 501
Cuts at: 3978 4251 5870 7582 96
Size: 273 1619 1712 138
Fragments arranged by size:
1712 1619 1470 1443 501 274 273 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3153 3185 7584 7616 3153
Size: 32 4399 32 3161
Fragments arranged by size:
4399 3161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3137 4135 1316
Size: 1821 998 4805
Fragments arranged by size:
4805 1821 998
XbaI T'CTAG_A
Cuts at: 2560 4960 2560
Size: 2400 5224
XhoI C'TCGA_G
Cuts at: 1943 4437 1943
Size: 2494 5130
XmaI C'CCGG_G
Cuts at: 1870 2125 4949 1870
Size: 255 2824 4545
Fragments arranged by size:
4545 2824 255
XmnI GAAnn'nnTTC
Cuts at: 2682 5753 2682
Size: 3071 4553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4080 4134 5432 276
Size: 53 83 186 936 2546 54 1298 2468
Fragments arranged by size:
2546 2468 1298 936 186 83 54 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI