Restriction map for
pFN21AB4082
(Circular) (Six-base) MAPSORT of: pFN21AB4082.seq Check: 3602 from: 1 to: 7387
pFN21AB4082 7387 bp
With 182 enzymes: SgfI *
September 11, 2009 17:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2601 5197 278
Size: 53 83 186 936 1065 2596 2468
Fragments arranged by size:
2596 2468 1065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 3372 4730 1937
Size: 981 454 1358 4594
Fragments arranged by size:
4594 1358 981 454
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 4029 5450 6834 7102 1632
Size: 1281 1116 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 1281 1116 268
AfeI AGC'GCT
Cuts at: 2152 2861 4456 2152
Size: 709 1595 5083
Fragments arranged by size:
5083 1595 709
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7190
AflIII A'CryG_T
Cuts at: 1203 3397 4659 6286 7263 1203
Size: 2194 1262 1627 977 1327
Fragments arranged by size:
2194 1627 1327 1262 977
AgeI A'CCGG_T
Cuts at: 6218 6218
Size: 7387
AhdI GACnn_n'nnGTC
Cuts at: 2326 6116 2326
Size: 3790 3597
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7387
AlwNI CAG_nnn'CTG
Cuts at: 5130 6702 5130
Size: 1572 5815
ApaI G_GGCC'C
Cuts at: 2362 2362
Size: 7387
ApaLI G'TGCA_C
Cuts at: 5444 6600 5444
Size: 1156 6231
ApoI r'AATT_y
Cuts at: 1493 2606 2885 3809 4070 4538 4696 4922 5074
Size: 1113 279 924 261 468 158 226 152
Cuts at: 5074 7183 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1113 924 468 279 261 226
158 152
AseI AT'TA_AT
Cuts at: 160 1032 5941 160
Size: 872 4909 1606
Fragments arranged by size:
4909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 4173 4712 1870
Size: 73 344 1886 539 4545
Fragments arranged by size:
4545 1886 539 344 73
AvrII C'CTAG_G
Cuts at: 2710 2710
Size: 7387
BamHI G'GATC_C
Cuts at: 3454 3649 4717 5084 3454
Size: 195 1068 367 5757
Fragments arranged by size:
5757 1068 367 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3845 4708 6163 618
Size: 325 217 627 2058 863 1455 1842
Fragments arranged by size:
2058 1842 1455 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2362 3076 4706 1391
Size: 971 714 1630 4072
Fragments arranged by size:
4072 1630 971 714
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 2450 3762 4017 928
Size: 971 142 409 1312 255 4298
Fragments arranged by size:
4298 1312 971 409 255 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5578 5612 1903
Size: 34 3641 34 3678
Fragments arranged by size:
3678 3641 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5279 6489 5279
Size: 1210 6177
BclI T'GATC_A
Cuts at: 1548 3930 1548
Size: 2382 5005
BfrBI ATG'CAT
Cuts at: 2788 2788
Size: 7387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5998 136
Size: 107 122 71 631 605 4326 1525
Fragments arranged by size:
4326 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7382 7382
Size: 7387
BlpI GC'TnA_GC
Cuts at: 3025 4318 4804 3025
Size: 1293 486 5608
Fragments arranged by size:
5608 1293 486
Bme1580I G_kGCm'C
Cuts at: 1790 2362 3045 5448 6604 1790
Size: 572 683 2403 1156 2573
Fragments arranged by size:
2573 2403 1156 683 572
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 5118 6076
Size: 416 53 83 399 24 858 2843 958
Cuts at: 6076 442
Size: 1753
Fragments arranged by size:
2843 1753 958 858 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3036 3067 4232 6047 1239
Size: 1797 31 1165 1815 2579
Fragments arranged by size:
2579 1815 1797 1165 31
Bpu10I CC'TnA_GC
Cuts at: 4466 4466
Size: 7387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2707 5512 6377 6675 2707
Size: 2805 865 298 3419
Fragments arranged by size:
3419 2805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6050 882
Size: 617 4551 2219
Fragments arranged by size:
4551 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 5178 493
Size: 711 1023 2951 2702
Fragments arranged by size:
2951 2702 1023 711
BsaBI GATnn'nnATC
Cuts at: 7381 7381
Size: 7387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 5194 5576 6211
Size: 53 83 186 936 1065 2596 382 635
Cuts at: 6211 275
Size: 1451
Fragments arranged by size:
2596 1451 1065 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4055 5820 6218 6492 6639 7050 4055
Size: 1765 398 274 147 411 4392
Fragments arranged by size:
4392 1765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2995 3025 3690 3720 1178
Size: 30 165 30 1592 30 665 30 4845
Fragments arranged by size:
4845 1592 665 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2992 1186
Size: 1806 5581
BseYI C'CCAG_C
Cuts at: 2431 2862 6590 7086 2431
Size: 431 3728 496 2732
Fragments arranged by size:
3728 2732 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2189 2348 2429 1163
Size: 573 423 30 159 81 6121
Fragments arranged by size:
6121 573 423 159 81 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4837 5598 6626 1540
Size: 275 186 2836 761 1028 2301
Fragments arranged by size:
2836 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2148 3076 5448 5533 6604 2040
Size: 108 928 2372 85 1071 2823
Fragments arranged by size:
2823 2372 1071 928 108 85
BsmI GAATG_Cn'
Cuts at: 2908 4898 4991 2908
Size: 1990 93 5304
Fragments arranged by size:
5304 1990 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2148 2362 3045 3076 4706 5448
Size: 399 250 108 214 683 31 1630 742
Cuts at: 5448 5533 6604 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1630 1071 742 683 399 250 214
108 85 31
BspEI T'CCGG_A
Cuts at: 7050 7050
Size: 7387
BspHI T'CATG_A
Cuts at: 2124 3579 3600 5276 7006 2124
Size: 1455 21 1676 1730 2505
Fragments arranged by size:
2505 1730 1676 1455 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3717 4742 844
Size: 2873 1025 3489
Fragments arranged by size:
3489 2873 1025
BsrBI CCG'CTC
Cuts at: 1394 1823 5274 1394
Size: 429 3451 3507
Fragments arranged by size:
3507 3451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3253 4477 5882 6056 65
Size: 1183 2005 1224 1405 174 1396
Fragments arranged by size:
2005 1405 1396 1224 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6031 6218 1700
Size: 110 4221 187 2869
Fragments arranged by size:
4221 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3315 96
Size: 3219 4168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7387
BssSI C'ACGA_G
Cuts at: 3774 5447 6459 3774
Size: 1673 1012 4702
Fragments arranged by size:
4702 1673 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 3035 794
Size: 1241 1000 5146
Fragments arranged by size:
5146 1241 1000
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstXI CCAn_nnnn'nTGG
Cuts at: 4525 4525
Size: 7387
BstYI r'GATC_y
Cuts at: 1972 3454 3649 4082 4717 5084 5469 5486 6938
Size: 1482 195 433 635 367 385 17 1452
Cuts at: 6938 7024 7036 7382 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1482 1452 635 433 385 367 346
195 86 17 12
BstZ17I GTA'TAC
Cuts at: 2919 2919
Size: 7387
Bsu36I CC'TnA_GG
Cuts at: 2975 2975
Size: 7387
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5896
Fragments arranged by size:
5896 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6648
Fragments arranged by size:
6648 683 56
BtsI GCAGTG_nn'
Cuts at: 2553 4903 5697 5717 2553
Size: 2350 794 20 4223
Fragments arranged by size:
4223 2350 794 20
ClaI AT'CG_AT
Cuts at: 1554 3153 1554
Size: 1599 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2562 2597 529
Size: 35 1998 35 5319
Fragments arranged by size:
5319 1998 35 35
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2669 6394 809
Size: 507 1353 3725 1802
Fragments arranged by size:
3725 1802 1353 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4515 4834
Size: 54 1425 50 275 18 177 2508 319
Cuts at: 4834 5723 7352 8
Size: 889 1629 43
Fragments arranged by size:
2508 1629 1425 889 319 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4834 1537
Size: 275 3022 4090
Fragments arranged by size:
4090 3022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5317 1353
Size: 3964 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5974 6348 6494 387
Size: 1064 138 4385 374 146 1280
Fragments arranged by size:
4385 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3074 3074
Size: 7387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2913 3036 3067 4029 4232 5450 6047
Size: 393 1281 123 31 962 203 1218 597
Cuts at: 6047 6834 7102 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1281 1218 962 787 597 393 268
203 123 31
EcoO109I rG'GnC_Cy
Cuts at: 2358 2359 4044 2358
Size: 1 1685 5701
Fragments arranged by size:
5701 1685 1
EcoRI G'AATT_C
Cuts at: 2606 2885 4538 4696 5074 2606
Size: 279 1653 158 378 4919
Fragments arranged by size:
4919 1653 378 279 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 4219 2671
Size: 1548 5839
FspI TGC'GCA
Cuts at: 5893 5893
Size: 7387
HaeII r_GCGC'y
Cuts at: 1678 2154 2448 2863 4458 6534 1678
Size: 476 294 415 1595 2076 2531
Fragments arranged by size:
2531 2076 1595 476 415 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2505 2537 3242
Size: 32 135 32 740 32 161 32 705
Cuts at: 3242 3274 3976 4008 4441 4473 6028 6060 6102
Size: 32 702 32 433 32 1555 32 42
Cuts at: 6102 6134 1373
Size: 32 2626
Fragments arranged by size:
2626 1555 740 705 702 433 161 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2824 4218 4731 4977 669
Size: 988 281 886 1394 513 246 3079
Fragments arranged by size:
3079 1394 988 886 513 281 246
HindIII A'AGCT_T
Cuts at: 748 2961 748
Size: 2213 5174
HpaI GTT'AAC
Cuts at: 4977 4977
Size: 7387
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 4986 4986
Size: 7387
MluI A'CGCG_T
Cuts at: 7263 7263
Size: 7387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3883 4480 6501 6685 1305
Size: 2578 597 2021 184 2007
Fragments arranged by size:
2578 2021 2007 597 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 4517 10
Size: 54 1425 343 177 2508 2880
Fragments arranged by size:
2880 2508 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3155 3281 4523 5345 5704 5863
Size: 648 1257 732 126 1242 822 359 159
Cuts at: 5863 518
Size: 2042
Fragments arranged by size:
2042 1257 1242 822 732 648 359 159
126
MspA1I CmG'CkG
Cuts at: 1606 4130 4803 5480 6628 6873 1606
Size: 2524 673 677 1148 245 2120
Fragments arranged by size:
2524 2120 1148 677 673 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5896
Fragments arranged by size:
5896 939 552
NdeI CA'TA_TG
Cuts at: 387 3307 4039 387
Size: 2920 732 3735
Fragments arranged by size:
3735 2920 732
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7387
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NsiI A_TGCA'T
Cuts at: 2790 2790
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 2743 3401 4745 6290 1132
Size: 97 1514 658 1344 1545 2229
Fragments arranged by size:
2229 1545 1514 1344 658 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7387
PciI A'CATG_T
Cuts at: 3397 6286 3397
Size: 2889 4498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7387
PfoI T'CCnGG_A
Cuts at: 2999 4433 2999
Size: 1434 5953
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5456 5488 6969 7001 5456
Size: 32 1481 32 5842
Fragments arranged by size:
5842 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4044 4044
Size: 7387
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7387
PsiI TTA'TAA
Cuts at: 4957 4957
Size: 7387
PspOMI G'GGCC_C
Cuts at: 2358 2358
Size: 7387
PstI C_TGCA'G
Cuts at: 830 4739 830
Size: 3909 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7387
SacI G_AGCT'C
Cuts at: 3076 3076
Size: 7387
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SanDI GG'GwC_CC
Cuts at: 4044 4044
Size: 7387
SbfI CC_TGCA'GG
Cuts at: 4739 4739
Size: 7387
ScaI AGT'ACT
Cuts at: 5635 7347 5635
Size: 1712 5675
SfcI C'TryA_G
Cuts at: 826 1045 4735 5870 6551 6742 826
Size: 219 3690 1135 681 191 1471
Fragments arranged by size:
3690 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7387
SmaI CCC'GGG
Cuts at: 1872 4714 1872
Size: 2842 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2287 2722 5491 6392 6654 820
Size: 197 926 344 435 2769 901 262 1553
Fragments arranged by size:
2769 1553 926 901 435 344 262 197
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 4745 1132
Size: 3613 3774
SspI AAT'ATT
Cuts at: 5 52 3112 3819 5311 5
Size: 47 3060 707 1492 2081
Fragments arranged by size:
3060 2081 1492 707 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7387
StyI C'CwwG_G
Cuts at: 513 1065 2004 2710 3840 4188 4425 4826 513
Size: 552 939 706 1130 348 237 401 3074
Fragments arranged by size:
3074 1130 939 706 552 401 348 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2854 3833 4607 5414 5431 5584 5769
Size: 1255 1274 979 774 807 17 153 185
Cuts at: 5769 6214 325
Size: 445 1498
Fragments arranged by size:
1498 1274 1255 979 807 774 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3091 3315 4230
Size: 274 80 33 51 1443 1114 224 915
Cuts at: 4230 5633 7345 96
Size: 1403 1712 138
Fragments arranged by size:
1712 1443 1403 1114 915 274 224 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2995 3027 3430 3462 7347 7379 2225
Size: 32 738 32 403 32 3885 32 2233
Fragments arranged by size:
3885 2233 738 403 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7387
XbaI T'CTAG_A
Cuts at: 4723 4723
Size: 7387
XhoI C'TCGA_G
Cuts at: 1943 2287 1943
Size: 344 7043
XmaI C'CCGG_G
Cuts at: 1870 4712 1870
Size: 2842 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 5516 2207
Size: 3309 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2599 5195 276
Size: 53 83 186 936 1065 2596 2468
Fragments arranged by size:
2596 2468 1065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BplI BsiWI
BsmBI DraIII EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI