Restriction map for
pFN21AB4080
(Circular) (Six-base) MAPSORT of: pFN21AB4080.seq Check: 6130 from: 1 to: 5719
pFN21AB4080 5719 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2680 3062 1937
Size: 743 382 4594
Fragments arranged by size:
4594 743 382
Acc65I G'GTAC_C
Cuts at: 2618 3040 2618
Size: 422 5297
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 5166 5434 1632
Size: 2150 1384 268 1917
Fragments arranged by size:
2150 1917 1384 268
AfeI AGC'GCT
Cuts at: 2309 2309
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2088 2142 4618 5595 1203
Size: 885 54 2476 977 1327
Fragments arranged by size:
2476 1327 977 885 54
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 2246 2508 2517 2630 2781 2920 3028 3254
Size: 753 262 9 113 151 139 108 226
Cuts at: 3254 3406 5515 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 753 262 226 152 151 139
113 108 9
AseI AT'TA_AT
Cuts at: 160 1032 2595 4273 160
Size: 872 1563 1678 1606
Fragments arranged by size:
1678 1606 1563 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2844 3044 1870
Size: 73 901 200 4545
Fragments arranged by size:
4545 901 200 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2394 2427 2394
Size: 33 5686
BamHI G'GATC_C
Cuts at: 3049 3416 3049
Size: 367 5352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 3040 4495 618
Size: 325 217 627 831 422 1455 1842
Fragments arranged by size:
1842 1455 831 627 422 325 217
BanII G_rGCy'C
Cuts at: 1391 2326 3038 1391
Size: 935 712 4072
Fragments arranged by size:
4072 935 712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2486 928
Size: 971 587 4161
Fragments arranged by size:
4161 971 587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2149 3611 4821 2149
Size: 1462 1210 3047
Fragments arranged by size:
3047 1462 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2961 5714 2961
Size: 2753 2966
BlpI GC'TnA_GC
Cuts at: 2562 3136 2562
Size: 574 5145
Bme1580I G_kGCm'C
Cuts at: 1790 3780 4936 1790
Size: 1990 1156 2573
Fragments arranged by size:
2573 1990 1156
BmgBI CAC'GTC
Cuts at: 1318 2091 1318
Size: 773 4946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 2398 3450
Size: 416 53 83 399 24 678 303 1052
Cuts at: 3450 4408 442
Size: 958 1753
Fragments arranged by size:
1753 1052 958 678 416 399 303 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4379 1239
Size: 3140 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2391 2552 3844 4709 5007 2391
Size: 161 1292 865 298 3103
Fragments arranged by size:
3103 1292 865 298 161
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 1178
Size: 30 165 30 1458 30 4006
Fragments arranged by size:
4006 1458 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 4922 5418 4922
Size: 496 5223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 4465
Fragments arranged by size:
4465 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 3865 4936 3780
Size: 85 1071 4563
Fragments arranged by size:
4563 1071 85
BsmI GAATG_Cn'
Cuts at: 2649 3230 3323 2649
Size: 581 93 5045
Fragments arranged by size:
5045 581 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2326 3038 3780 3865 4936 1391
Size: 399 536 712 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 536 399 85
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2388 2786 2388
Size: 398 5321
BstYI r'GATC_y
Cuts at: 1972 2961 3049 3416 3801 3818 5270 5356 5368
Size: 989 88 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 989 385 367 346 88 86
17 12
BstZ17I GTA'TAC
Cuts at: 2681 2681
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2632 3235 4029 4049 2188
Size: 444 603 794 20 3858
Fragments arranged by size:
3858 794 603 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684
Size: 54 1425 50 275 18 1336 889 1629
Cuts at: 5684 8
Size: 43
Fragments arranged by size:
1629 1425 1336 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3649 1353
Size: 2296 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3782 4379 5166 5434 1239
Size: 393 2150 597 787 268 1524
Fragments arranged by size:
2150 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2508 2517 3028 3406 2508
Size: 9 511 378 4821
Fragments arranged by size:
4821 511 378 9
EcoRV GAT'ATC
Cuts at: 2191 2191
Size: 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2326 2281
Size: 45 5674
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2311 4866 1678
Size: 633 2555 2531
Fragments arranged by size:
2555 2531 633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2473 2505 4360 4392 4434
Size: 32 135 32 901 32 1855 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1855 901 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2200 748
Size: 1452 4267
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 2622 3044 2622
Size: 422 5297
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 3677 4036 4195 518
Size: 648 1209 1302 359 159 2042
Fragments arranged by size:
2042 1302 1209 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2806 2934 3135 3812 4960 5205 1606
Size: 1200 128 201 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 245 201 128
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2146 2890 3077 4622 1132
Size: 97 917 744 187 1545 2229
Fragments arranged by size:
2229 1545 917 744 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 2142 4618 2142
Size: 2476 3243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspXI vC'TCGA_Gb
Cuts at: 2844 2844
Size: 5719
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2934 2934
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3002 3967 5679 3002
Size: 965 1712 3042
Fragments arranged by size:
3042 1712 965
SfcI C'TryA_G
Cuts at: 826 1045 3067 4202 4883 5074 826
Size: 219 2022 1135 681 191 1471
Fragments arranged by size:
2022 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2406 2567 2844 3823 4724 4986
Size: 197 926 463 161 277 979 901 262
Cuts at: 4986 820
Size: 1553
Fragments arranged by size:
1553 979 926 901 463 277 262 197
161
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 3158 513
Size: 552 2093 3074
Fragments arranged by size:
3074 2093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3000 3965 5677
Size: 274 80 33 51 1443 1023 965 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1443 1023 965 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 2844 1943
Size: 901 4818
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 2774 3848 2774
Size: 1074 4645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI