Restriction map for
pFN21AB4066
(Circular) (Six-base) MAPSORT of: pFN21AB4066.seq Check: 6253 from: 1 to: 5977
pFN21AB4066 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3125 3298 3125
Size: 173 5804
AclI AA'CG_TT
Cuts at: 1563 2049 4104 4477 1563
Size: 486 2055 373 3063
Fragments arranged by size:
3063 2055 486 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 4040 5424 5692 1632
Size: 602 1806 1384 268 1917
Fragments arranged by size:
1917 1806 1384 602 268
AflII C'TTAA_G
Cuts at: 820 1017 2953 820
Size: 197 1936 3844
Fragments arranged by size:
3844 1936 197
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2167 3720 5292 2167
Size: 1553 1572 2852
Fragments arranged by size:
2852 1572 1553
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2695 2738 2993 3078 3286 3512 3664 5773
Size: 1202 43 255 85 208 226 152 2109
Cuts at: 5773 1493
Size: 1697
Fragments arranged by size:
2109 1697 1202 255 226 208 152 85
43
AseI AT'TA_AT
Cuts at: 160 1032 3168 4531 160
Size: 872 2136 1363 1606
Fragments arranged by size:
2136 1606 1363 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
BamHI G'GATC_C
Cuts at: 2662 3307 3674 2662
Size: 645 367 4965
Fragments arranged by size:
4965 645 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3125 3298 4753 618
Size: 325 217 627 1338 173 1455 1842
Fragments arranged by size:
1842 1455 1338 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2142 2198 3296 1391
Size: 751 56 1098 4072
Fragments arranged by size:
4072 1098 751 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4168 4202 1903
Size: 34 50 34 2147 34 3678
Fragments arranged by size:
3678 2147 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2646 4588 136
Size: 107 122 71 631 605 974 1942 1525
Fragments arranged by size:
1942 1525 974 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2905 5972 2905
Size: 3067 2910
BlpI GC'TnA_GC
Cuts at: 2759 3394 2759
Size: 635 5342
Bme1580I G_kGCm'C
Cuts at: 1790 4038 5194 1790
Size: 2248 1156 2573
Fragments arranged by size:
2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 3708 4666
Size: 416 53 83 399 24 1844 447 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1844 1753 958 447 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2006 2147 2879 4637 1239
Size: 767 141 732 1758 2579
Fragments arranged by size:
2579 1758 767 732 141
Bpu10I CC'TnA_GC
Cuts at: 2259 2349 2259
Size: 90 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2460 4102 4967 5265 2460
Size: 1642 865 298 3172
Fragments arranged by size:
3172 1642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2122 2368 2846 4640 882
Size: 617 623 246 478 1794 2219
Fragments arranged by size:
2219 1794 623 617 478 246
BsaAI yAC'GTr
Cuts at: 493 1204 2067 3768 493
Size: 711 863 1701 2702
Fragments arranged by size:
2702 1701 863 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2305 4410 4808 5082 5229 5640 2305
Size: 2105 398 274 147 411 2642
Fragments arranged by size:
2642 2105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 1186
Size: 964 5013
BseYI C'CCAG_C
Cuts at: 2862 5180 5676 2862
Size: 2318 496 3163
Fragments arranged by size:
3163 2318 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 1163
Size: 573 693 4711
Fragments arranged by size:
4711 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2198 3296 4038 4123 5194 1391
Size: 399 352 56 1098 742 85 1071 2174
Fragments arranged by size:
2174 1098 1071 742 399 352 85 56
BspEI T'CCGG_A
Cuts at: 2305 5640 2305
Size: 3335 2642
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3189 4472 4646 65
Size: 1183 1941 1283 174 1396
Fragments arranged by size:
1941 1396 1283 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 2551 2824 96
Size: 2455 273 3249
Fragments arranged by size:
3249 2455 273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2755 3243 794
Size: 1961 488 3528
Fragments arranged by size:
3528 1961 488
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2662 2905 3307 3674 4059 4076 5528 5614
Size: 690 243 402 367 385 17 1452 86
Cuts at: 5614 5626 5972 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 690 402 385 367 346 243
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2201 2599 513
Size: 552 1136 398 3891
Fragments arranged by size:
3891 1136 552 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3254 3493 4287 4307 3254
Size: 239 794 20 4924
Fragments arranged by size:
4924 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942
Size: 54 1425 50 275 18 1594 889 1629
Cuts at: 5942 8
Size: 43
Fragments arranged by size:
1629 1594 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 3907 1353
Size: 857 1697 3423
Fragments arranged by size:
3423 1697 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2110 4564 4938 5084 387
Size: 1064 138 521 2454 374 146 1280
Fragments arranged by size:
2454 1280 1064 521 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2147 2234 2879 4040 4637 5424
Size: 393 374 141 87 645 1161 597 787
Cuts at: 5424 5692 1239
Size: 268 1524
Fragments arranged by size:
1524 1161 787 645 597 393 374 268
141 87
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 2841 4483 2841
Size: 1642 4335
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2403 4618 4650 4692
Size: 32 135 32 799 32 2215 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 2215 799 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3129 3302 3129
Size: 173 5804
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3040 5091 5275 1305
Size: 1735 2051 184 2007
Fragments arranged by size:
2051 2007 1735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2451 3935 4294 4453 518
Size: 648 1285 1484 359 159 2042
Fragments arranged by size:
2042 1484 1285 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2496 2766 2866 3393 4070 5218 5463 1606
Size: 890 270 100 527 677 1148 245 2120
Fragments arranged by size:
2120 1148 890 677 527 270 245 100
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5425
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2825 2846 3335 4880 1132
Size: 97 1596 21 489 1545 2229
Fragments arranged by size:
2229 1596 1545 489 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 3206 1108
Size: 1416 682 3879
Fragments arranged by size:
3879 1416 682
PfoI T'CCnGG_A
Cuts at: 2022 2022
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 2067 2067
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2751 3329 830
Size: 1921 578 3478
Fragments arranged by size:
3478 1921 578
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2766 2866 2766
Size: 100 5877
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2210 2210
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2747 2881 3161 3325 4460 5141 5332
Size: 219 1702 134 280 164 1135 681 191
Cuts at: 5332 826
Size: 1471
Fragments arranged by size:
1702 1471 1135 681 280 219 191 164
134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2475 2953 4081 4982 5244 820
Size: 197 926 532 478 1128 901 262 1553
Fragments arranged by size:
1553 1128 926 901 532 478 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2257 2649 1517
Size: 740 392 4845
Fragments arranged by size:
4845 740 392
StyI C'CwwG_G
Cuts at: 513 1065 3416 513
Size: 552 2351 3074
Fragments arranged by size:
3074 2351 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2709 2876 4004 4021 4174 4359 4804
Size: 1255 1129 167 1128 17 153 185 445
Cuts at: 4804 325
Size: 1498
Fragments arranged by size:
1498 1255 1129 1128 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 2824 4223
Size: 274 80 33 51 1443 574 273 1399
Cuts at: 4223 5935 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1399 574 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 2947 3313 2947
Size: 366 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2289 2289
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 2329 3150 4106 2329
Size: 821 956 4200
Fragments arranged by size:
4200 956 821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI