Restriction map for
pFN21AB4057
(Circular) (Six-base) MAPSORT of: pFN21AB4057.seq Check: 3787 from: 1 to: 6766
pFN21AB4057 6766 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3477 4576 278
Size: 53 83 186 936 1941 1099 2468
Fragments arranged by size:
2468 1941 1099 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 2658 3210 4109 1937
Size: 229 492 552 899 4594
Fragments arranged by size:
4594 899 552 492 229
Acc65I G'GTAC_C
Cuts at: 4087 4087
Size: 6766
AclI AA'CG_TT
Cuts at: 1563 4893 5266 1563
Size: 3330 373 3063
Fragments arranged by size:
3330 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2636 3296 4043 4829 6213 6481 1632
Size: 532 472 660 747 786 1384 268 1917
Fragments arranged by size:
1917 1384 786 747 660 532 472 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6569
AflIII A'CryG_T
Cuts at: 1203 5665 6642 1203
Size: 4462 977 1327
Fragments arranged by size:
4462 1327 977
AgeI A'CCGG_T
Cuts at: 5597 5597
Size: 6766
AhdI GACnn_n'nnGTC
Cuts at: 3232 5495 3232
Size: 2263 4503
AleI CACnn'nnGTG
Cuts at: 1166 2279 1166
Size: 1113 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6766
AlwNI CAG_nnn'CTG
Cuts at: 3073 4509 6081 3073
Size: 1436 1572 3758
Fragments arranged by size:
3758 1572 1436
ApaI G_GGCC'C
Cuts at: 3570 3700 3570
Size: 130 6636
ApaLI G'TGCA_C
Cuts at: 3239 4823 5979 3239
Size: 1584 1156 4026
Fragments arranged by size:
4026 1584 1156
ApoI r'AATT_y
Cuts at: 1493 2545 4075 4301 4453 6562 1493
Size: 1052 1530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1530 1052 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5320 160
Size: 872 4288 1606
Fragments arranged by size:
4288 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
AvaI C'yCGr_G
Cuts at: 1870 1943 3569 4091 1870
Size: 73 1626 522 4545
Fragments arranged by size:
4545 1626 522 73
BamHI G'GATC_C
Cuts at: 2384 2568 3216 3432 3780 4096 4463 2384
Size: 184 648 216 348 316 367 4687
Fragments arranged by size:
4687 648 367 348 316 216 184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2430 2871 3578 4087 5542
Size: 325 217 627 643 441 707 509 1455
Cuts at: 5542 618
Size: 1842
Fragments arranged by size:
1842 1455 707 643 627 509 441 325
217
BanII G_rGCy'C
Cuts at: 1391 2516 3570 3700 3795 4085 1391
Size: 1125 1054 130 95 290 4072
Fragments arranged by size:
4072 1125 1054 290 130 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2833 3911 928
Size: 971 934 1078 3783
Fragments arranged by size:
3783 1078 971 934
BbvCI CC'TCA_GC
Cuts at: 2354 2867 3504 3819 2354
Size: 513 637 315 5301
Fragments arranged by size:
5301 637 513 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4957 4991 1903
Size: 34 3020 34 3678
Fragments arranged by size:
3678 3020 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4658 5868 4658
Size: 1210 5556
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6766
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 2852 5377
Size: 107 122 71 631 605 450 730 2525
Cuts at: 5377 136
Size: 1525
Fragments arranged by size:
2525 1525 730 631 605 450 122 107
71
BglII A'GATC_T
Cuts at: 6761 6761
Size: 6766
BlpI GC'TnA_GC
Cuts at: 4183 4183
Size: 6766
Bme1580I G_kGCm'C
Cuts at: 1790 2435 3243 3538 3570 3700 4827 5983 1790
Size: 645 808 295 32 130 1127 1156 2573
Fragments arranged by size:
2573 1156 1127 808 645 295 130 32
BmgBI CAC'GTC
Cuts at: 1318 3205 1318
Size: 1887 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3116 4497 5455
Size: 416 53 83 399 24 1699 1381 958
Cuts at: 5455 442
Size: 1753
Fragments arranged by size:
1753 1699 1381 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3367 3631 3648 3367
Size: 264 17 6485
Fragments arranged by size:
6485 264 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2066 2381 3626 3776 5426 1239
Size: 821 6 315 1245 150 1650 2579
Fragments arranged by size:
2579 1650 1245 821 315 150 6
Bpu10I CC'TnA_GC
Cuts at: 2354 2867 3155 3504 3819 2354
Size: 513 288 349 315 5301
Fragments arranged by size:
5301 513 349 315 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4891 5756 6054 4891
Size: 865 298 5603
Fragments arranged by size:
5603 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2055 5429 882
Size: 617 543 13 3374 2219
Fragments arranged by size:
3374 2219 617 543 13
BsaAI yAC'GTr
Cuts at: 493 1204 2662 4557 493
Size: 711 1458 1895 2702
Fragments arranged by size:
2702 1895 1458 711
BsaBI GATnn'nnATC
Cuts at: 3779 6760 3779
Size: 2981 3785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2748 3474 4573 4955
Size: 53 83 186 936 1215 726 1099 382
Cuts at: 4955 5590 275
Size: 635 1451
Fragments arranged by size:
1451 1215 1099 936 726 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3777 4041 5199 5597 5871 6018 6429 3777
Size: 264 1158 398 274 147 411 4114
Fragments arranged by size:
4114 1158 411 398 274 264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6541
Fragments arranged by size:
6541 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2270 2480 3371 3425 3521 3692 3911
Size: 841 243 210 891 54 96 171 219
Cuts at: 3911 1186
Size: 4041
Fragments arranged by size:
4041 891 841 243 219 210 171 96
54
BseYI C'CCAG_C
Cuts at: 2197 2398 2434 2490 2716 2724 2835 3012 5969
Size: 201 36 56 226 8 111 177 2957
Cuts at: 5969 6465 2197
Size: 496 2498
Fragments arranged by size:
2957 2498 496 226 201 177 111 56
36 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 2281 2842 3847 1163
Size: 573 381 164 561 1005 4082
Fragments arranged by size:
4082 1005 573 561 381 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 2629 4216 4977 6005 1540
Size: 275 186 75 553 1587 761 1028 2301
Fragments arranged by size:
2301 1587 1028 761 553 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 3161 3243 4827 4912 5983 3161
Size: 82 1584 85 1071 3944
Fragments arranged by size:
3944 1584 1071 85 82
BsmI GAATG_Cn'
Cuts at: 4277 4370 4277
Size: 93 6673
BsmBI CGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 6766
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2435 2516 3161 3243 3538 3570 3700
Size: 399 645 81 645 82 295 32 130
Cuts at: 3700 3795 4085 4827 4912 5983 1391
Size: 95 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 645 645 399 295 290
130 95 85 82 81 32
BspEI T'CCGG_A
Cuts at: 3777 4041 6429 3777
Size: 264 2388 4114
Fragments arranged by size:
4114 2388 264
BspHI T'CATG_A
Cuts at: 4655 6385 4655
Size: 1730 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3597 3738 3972 4121 844
Size: 2207 546 141 234 149 3489
Fragments arranged by size:
3489 2207 546 234 149 141
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3310 3841 3973 4653 1394
Size: 429 647 840 531 132 680 3507
Fragments arranged by size:
3507 840 680 647 531 429 132
BsrDI GCAATG_nn'
Cuts at: 65 1248 2962 5261 5435 65
Size: 1183 1714 2299 174 1396
Fragments arranged by size:
2299 1714 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2624 3884 5410 5597 1700
Size: 110 382 432 1260 1526 187 2869
Fragments arranged by size:
2869 1526 1260 432 382 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6766
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6766
BssSI C'ACGA_G
Cuts at: 3704 4826 5838 3704
Size: 1122 1012 4632
Fragments arranged by size:
4632 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6766
BstBI TT'CG_AA
Cuts at: 5570 5570
Size: 6766
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6766
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2281
Size: 6766
BstYI r'GATC_y
Cuts at: 1972 2384 2568 2970 3216 3432 3558 3774 3780
Size: 412 184 402 246 216 126 216 6
Cuts at: 3780 3915 4096 4463 4848 4865 6317 6403 6415
Size: 135 181 367 385 17 1452 86 12
Cuts at: 6415 6761 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 412 402 385 367 346 246
216 216 184 181 135 126 86 17
12 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 2803 2850 3927 513
Size: 552 939 273 526 47 1077 3352
Fragments arranged by size:
3352 1077 939 552 526 273 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6027
Fragments arranged by size:
6027 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3469 4282 5076 5096 3007
Size: 462 813 794 20 4677
Fragments arranged by size:
4677 813 794 462 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6731
DraI TTT'AAA
Cuts at: 4070 4070
Size: 6766
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3473 5773 809
Size: 507 2157 2300 1802
Fragments arranged by size:
2300 2157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2056 2073
Size: 54 1425 50 275 18 177 49 17
Cuts at: 2073 2325 2517 2626 2827 3399 3423 4213 5102
Size: 252 192 109 201 572 24 790 889
Cuts at: 5102 6731 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 790 572 275 252 201
192 177 109 54 50 49 43 24
18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2626 4213 1537
Size: 275 261 553 1587 4090
Fragments arranged by size:
4090 1587 553 275 261
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2457 3089 3617 4696 1353
Size: 1070 34 632 528 1079 3423
Fragments arranged by size:
3423 1079 1070 632 528 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5353 5727 5873 387
Size: 1064 138 3764 374 146 1280
Fragments arranged by size:
3764 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2066 2164 2381 2636 3296 3626
Size: 393 428 6 98 217 255 660 330
Cuts at: 3626 3776 4043 4829 5426 6213 6481 1239
Size: 150 267 786 597 787 268 1524
Fragments arranged by size:
1524 787 786 660 597 428 393 330
268 267 255 217 150 98 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3373 3686 3893 3373
Size: 313 207 6246
Fragments arranged by size:
6246 313 207
EcoO109I rG'GnC_Cy
Cuts at: 3070 3348 3566 3697 3745 3070
Size: 278 218 131 48 6091
Fragments arranged by size:
6091 278 218 131 48
EcoRI G'AATT_C
Cuts at: 4075 4453 4075
Size: 378 6388
EcoRV GAT'ATC
Cuts at: 3319 3319
Size: 6766
FseI GG_CCGG'CC
Cuts at: 3888 3888
Size: 6766
FspI TGC'GCA
Cuts at: 5272 5272
Size: 6766
HaeII r_GCGC'y
Cuts at: 1678 2794 5913 1678
Size: 1116 3119 2531
Fragments arranged by size:
3119 2531 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 3218 3250 5407
Size: 32 135 32 448 32 1166 32 2157
Cuts at: 5407 5439 5481 5513 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2157 1166 448 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2602 2689 4110 4356 669
Size: 988 281 664 87 1421 246 3079
Fragments arranged by size:
3079 1421 988 664 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6766
HpaI GTT'AAC
Cuts at: 4356 4356
Size: 6766
KpnI G_GTAC'C
Cuts at: 4091 4091
Size: 6766
MfeI C'AATT_G
Cuts at: 4365 4365
Size: 6766
MluI A'CGCG_T
Cuts at: 6642 6642
Size: 6766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3702 5880 6064 1305
Size: 2397 2178 184 2007
Fragments arranged by size:
2397 2178 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2829 3425 10
Size: 54 1425 343 997 596 3351
Fragments arranged by size:
3351 1425 997 596 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 3455 3689 4724 5083 5242 518
Size: 648 1113 1176 234 1035 359 159 2042
Fragments arranged by size:
2042 1176 1113 1035 648 359 234 159
MspA1I CmG'CkG
Cuts at: 1606 2438 3503 4182 4859 6007 6252 1606
Size: 832 1065 679 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 832 679 677 245
NaeI GCC'GGC
Cuts at: 2626 3886 2626
Size: 1260 5506
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5275
Fragments arranged by size:
5275 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6766
NgoMIV G'CCGG_C
Cuts at: 2624 3884 2624
Size: 1260 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6766
NotI GC'GGCC_GC
Cuts at: 4213 4213
Size: 6766
NspI r_CATG'y
Cuts at: 1132 1229 4124 5669 1132
Size: 97 2895 1545 2229
Fragments arranged by size:
2895 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6766
PciI A'CATG_T
Cuts at: 5665 5665
Size: 6766
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2763 3994 1108
Size: 1655 1231 3880
Fragments arranged by size:
3880 1655 1231
PfoI T'CCnGG_A
Cuts at: 2762 2762
Size: 6766
PmeI GTTT'AAAC
Cuts at: 4070 4070
Size: 6766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4835 4867 6348 6380 4835
Size: 32 1481 32 5221
Fragments arranged by size:
5221 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3348 3745 3348
Size: 397 6369
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6766
PsiI TTA'TAA
Cuts at: 4336 4336
Size: 6766
PspOMI G'GGCC_C
Cuts at: 3566 3696 3566
Size: 130 6636
PstI C_TGCA'G
Cuts at: 830 2313 3048 3834 4118 830
Size: 1483 735 786 284 3478
Fragments arranged by size:
3478 1483 786 735 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6766
SalI G'TCGA_C
Cuts at: 1936 4108 1936
Size: 2172 4594
SapI GCTCTTCn'nnn_
Cuts at: 2423 3089 3617 2423
Size: 666 528 5572
Fragments arranged by size:
5572 666 528
SbfI CC_TGCA'GG
Cuts at: 4118 4118
Size: 6766
ScaI AGT'ACT
Cuts at: 2948 5014 6726 2948
Size: 2066 1712 2988
Fragments arranged by size:
2988 2066 1712
SfcI C'TryA_G
Cuts at: 826 1045 2309 3044 3830 4114 5249 5930 6121
Size: 219 1264 735 786 284 1135 681 191
Cuts at: 6121 826
Size: 1471
Fragments arranged by size:
1471 1264 1135 786 735 681 284 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6766
SmaI CCC'GGG
Cuts at: 1872 4093 1872
Size: 2221 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4870 5771 6033 820
Size: 197 926 2927 901 262 1553
Fragments arranged by size:
2927 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4557 493
Size: 4064 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6766
SphI G_CATG'C
Cuts at: 1132 4124 1132
Size: 2992 3774
SspI AAT'ATT
Cuts at: 5 52 4690 5
Size: 47 4638 2081
Fragments arranged by size:
4638 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 3368 1517
Size: 849 1002 4915
Fragments arranged by size:
4915 1002 849
StyI C'CwwG_G
Cuts at: 513 1065 2004 2223 2540 3784 4205 513
Size: 552 939 219 317 1244 421 3074
Fragments arranged by size:
3074 1244 939 552 421 317 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3004 3338 4793 4810 4963 5148
Size: 1255 924 500 334 1455 17 153 185
Cuts at: 5148 5593 325
Size: 445 1498
Fragments arranged by size:
1498 1455 1255 924 500 445 334 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2946 5012 6724
Size: 274 80 33 51 1443 969 2066 1712
Cuts at: 6724 96
Size: 138
Fragments arranged by size:
2066 1712 1443 969 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6726 6758 6726
Size: 32 6734
Tth111I GACn'n_nGTC
Cuts at: 1316 2747 1316
Size: 1431 5335
XbaI T'CTAG_A
Cuts at: 2593 4102 2593
Size: 1509 5257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2395 3227 2395
Size: 832 5934
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6766
XmaI C'CCGG_G
Cuts at: 1870 4091 1870
Size: 2221 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 4895 4037
Size: 858 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3475 4574 276
Size: 53 83 186 936 1941 1099 2468
Fragments arranged by size:
2468 1941 1099 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI