Restriction map for
pFN21AB4055
(Circular) (Six-base) MAPSORT of: pFN21AB4055.seq Check: 4124 from: 1 to: 5704
pFN21AB4055 5704 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2551 3514 278
Size: 53 83 186 936 1015 963 2468
Fragments arranged by size:
2468 1015 963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 2774 3767 5151 5419 1632
Size: 1053 89 993 1384 268 1917
Fragments arranged by size:
1917 1384 1053 993 268 89
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 2592 4433 2592
Size: 1841 3863
AleI CACnn'nnGTG
Cuts at: 1166 2738 1166
Size: 1572 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2284 1184
Size: 1100 4604
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 2110 2126 2330 2351 2378 3013 3239 3391
Size: 617 16 204 21 27 635 226 152
Cuts at: 3391 5500 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 635 617 226 204 152 27
21 16
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2940 3025 4480 618
Size: 325 217 627 1153 85 1455 1842
Fragments arranged by size:
1842 1455 1153 627 325 217 85
BanII G_rGCy'C
Cuts at: 1391 2862 2981 3023 1391
Size: 1471 119 42 4072
Fragments arranged by size:
4072 1471 119 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2893 928
Size: 971 994 3739
Fragments arranged by size:
3739 994 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BfrBI ATG'CAT
Cuts at: 2709 2709
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4315 136
Size: 107 122 71 631 605 2643 1525
Fragments arranged by size:
2643 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2810 3121 2810
Size: 311 5393
Bme1580I G_kGCm'C
Cuts at: 1790 2943 3765 4921 1790
Size: 1153 822 1156 2573
Fragments arranged by size:
2573 1156 1153 822
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3435 4393
Size: 416 53 83 399 24 1439 579 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1439 958 579 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4364 1239
Size: 3125 2579
Bpu10I CC'TnA_GC
Cuts at: 2882 2882
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 3511 3893 4528
Size: 53 83 186 936 1015 963 382 635
Cuts at: 4528 275
Size: 1451
Fragments arranged by size:
1451 1015 963 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2760 4137 4535 4809 4956 5367 2760
Size: 1377 398 274 147 411 3097
Fragments arranged by size:
3097 1377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2779 1186
Size: 1102 491 4111
Fragments arranged by size:
4111 1102 491
BseYI C'CCAG_C
Cuts at: 2040 4907 5403 2040
Size: 2867 496 2341
Fragments arranged by size:
2867 2341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2862 2943 2981 3023 3765 3850 4921
Size: 399 1072 81 38 42 742 85 1071
Cuts at: 4921 1391
Size: 2174
Fragments arranged by size:
2174 1072 1071 742 399 85 81 42
38
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 2487 3593 5323 2487
Size: 1106 1730 2868
Fragments arranged by size:
2868 1730 1106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 2949 96
Size: 2853 2851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2513 2696 794
Size: 1719 183 3802
Fragments arranged by size:
3802 1719 183
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2481 2536 3034 3401 3786 3803 5255 5341
Size: 509 55 498 367 385 17 1452 86
Cuts at: 5341 5353 5699 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 509 498 385 367 346 86
55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 2084 1554
Size: 530 5174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2947 2947
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2803 4711 809
Size: 507 1487 1908 1802
Fragments arranged by size:
1908 1802 1487 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2820 3151
Size: 54 1425 50 275 18 385 605 331
Cuts at: 3151 4040 5669 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 605 385 331 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2685 2774 3767 4364 5151 5419 1239
Size: 393 1053 89 993 597 787 268 1524
Fragments arranged by size:
1524 1053 993 787 597 393 268 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2139 2139
Size: 5704
EcoO109I rG'GnC_Cy
Cuts at: 2434 2434
Size: 5704
EcoRI G'AATT_C
Cuts at: 2110 2351 3013 3391 2110
Size: 241 662 378 4423
Fragments arranged by size:
4423 662 378 241
EcoRV GAT'ATC
Cuts at: 2326 2326
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2643 4851 1678
Size: 965 2208 2531
Fragments arranged by size:
2531 2208 965
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 2697 2729 4345
Size: 32 135 32 1006 32 87 32 1616
Cuts at: 4345 4377 4419 4451 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1616 1006 135 87 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2476 2564 4818 5002 1305
Size: 1171 88 2254 184 2007
Fragments arranged by size:
2254 2007 1171 184 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2217 2822 10
Size: 54 1425 343 385 605 2892
Fragments arranged by size:
2892 1425 605 385 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2738 3662 4021 4180 518
Size: 648 843 729 924 359 159 2042
Fragments arranged by size:
2042 924 843 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2838 2928 3120 3797 4945 5190 1606
Size: 1232 90 192 677 1148 245 2120
Fragments arranged by size:
2120 1232 1148 677 245 192 90
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4213
Fragments arranged by size:
4213 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NsiI A_TGCA'T
Cuts at: 2711 2711
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PfoI T'CCnGG_A
Cuts at: 2958 2958
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 2232 3274 2232
Size: 1042 4662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 5672
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2036 3952 5664 2036
Size: 1916 1712 2076
Fragments arranged by size:
2076 1916 1712
SfcI C'TryA_G
Cuts at: 826 1045 3052 4187 4868 5059 826
Size: 219 2007 1135 681 191 1471
Fragments arranged by size:
2007 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 2228 3628 5
Size: 47 2176 1400 2081
Fragments arranged by size:
2176 2081 1400 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2004 3143 513
Size: 552 939 1139 3074
Fragments arranged by size:
3074 1139 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2842 3731 3748 3901 4086 4531 325
Size: 1255 1262 889 17 153 185 445 1498
Fragments arranged by size:
1498 1262 1255 889 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 2949 3950
Size: 274 80 33 51 1443 57 915 1001
Cuts at: 3950 5662 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1001 915 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 5664 5696 2012
Size: 32 3620 32 2020
Fragments arranged by size:
3620 2020 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2897 1316
Size: 1581 4123
XbaI T'CTAG_A
Cuts at: 2374 3040 2374
Size: 666 5038
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2581 3833 2581
Size: 1252 4452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2549 3512 276
Size: 53 83 186 936 1015 963 2468
Fragments arranged by size:
2468 1015 963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI