Restriction map for
pFN21AB4041
(Circular) (Six-base) MAPSORT of: pFN21AB4041.seq Check: 2329 from: 1 to: 6094
pFN21AB4041 6094 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4157 5541 5809 1632
Size: 2525 1384 268 1917
Fragments arranged by size:
2525 1917 1384 268
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 6094
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 4993 5970 1203
Size: 3790 977 1327
Fragments arranged by size:
3790 1327 977
AgeI A'CCGG_T
Cuts at: 2969 4925 2969
Size: 1956 4138
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 2886 2970 3390 1166
Size: 1720 84 420 3870
Fragments arranged by size:
3870 1720 420 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 3837 5409 3837
Size: 1572 4522
ApaLI G'TGCA_C
Cuts at: 3073 4151 5307 3073
Size: 1078 1156 3860
Fragments arranged by size:
3860 1156 1078
ApoI r'AATT_y
Cuts at: 1493 2215 2389 2541 2793 2877 3213 3403 3629
Size: 722 174 152 252 84 336 190 226
Cuts at: 3629 3781 5890 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 722 336 252 226 190 174
152 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2931 3419 1870
Size: 73 988 488 4545
Fragments arranged by size:
4545 988 488 73
BamHI G'GATC_C
Cuts at: 3381 3424 3791 3381
Size: 43 367 5684
Fragments arranged by size:
5684 367 43
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 3415 4870 618
Size: 325 217 627 564 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 564 325 217
BanII G_rGCy'C
Cuts at: 1391 2603 2694 3413 1391
Size: 1212 91 719 4072
Fragments arranged by size:
4072 1212 719 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2759 928
Size: 971 860 4263
Fragments arranged by size:
4263 971 860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3039 3986 5196 3039
Size: 947 1210 3937
Fragments arranged by size:
3937 1210 947
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4705 136
Size: 107 122 71 631 605 3033 1525
Fragments arranged by size:
3033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 3077 4155 5311 1790
Size: 1287 1078 1156 2573
Fragments arranged by size:
2573 1287 1156 1078
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 2559 2727
Size: 416 53 83 399 24 690 452 168
Cuts at: 2727 3825 4783 442
Size: 1098 958 1753
Fragments arranged by size:
1753 1098 958 690 452 416 399 168
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2272 4754 1239
Size: 893 140 2482 2579
Fragments arranged by size:
2579 2482 893 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 4219 5084 5382 2587
Size: 1632 865 298 3299
Fragments arranged by size:
3299 1632 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2137 4757 882
Size: 617 638 2620 2219
Fragments arranged by size:
2620 2219 638 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 2315 6088 2315
Size: 3773 2321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3901 4283 4918 275
Size: 53 83 186 936 2368 382 635 1451
Fragments arranged by size:
2368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2969 4527 4925 5199 5346 5757 2969
Size: 1558 398 274 147 411 3306
Fragments arranged by size:
3306 1558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5869
Fragments arranged by size:
5869 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5208
BseYI C'CCAG_C
Cuts at: 3015 5297 5793 3015
Size: 2282 496 3316
Fragments arranged by size:
3316 2282 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2694 3077 4155 4240 5311 2694
Size: 383 1078 85 1071 3477
Fragments arranged by size:
3477 1078 1071 383 85
BsmI GAATG_Cn'
Cuts at: 2741 3605 3698 2741
Size: 864 93 5137
Fragments arranged by size:
5137 864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2603 2694 3077 3413 4155 4240 5311
Size: 399 813 91 383 336 742 85 1071
Cuts at: 5311 1391
Size: 2174
Fragments arranged by size:
2174 1071 813 742 399 383 336 91
85
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 2191 3983 5713 2191
Size: 1792 1730 2572
Fragments arranged by size:
2572 1792 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2344 3449 844
Size: 1500 1105 3489
Fragments arranged by size:
3489 1500 1105
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2969 4738 4925 1700
Size: 110 1159 1769 187 2869
Fragments arranged by size:
2869 1769 1159 187 110
BsrGI T'GTAC_A
Cuts at: 96 3242 96
Size: 3146 2948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 3038 3122 3197 4154 5166 3038
Size: 84 75 957 1012 3966
Fragments arranged by size:
3966 1012 957 84 75
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 2961 3381 3424 3791 4176 4193 5645 5731
Size: 989 420 43 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 989 420 385 367 346 86
43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 3610 4404 4424 3610
Size: 794 20 5280
Fragments arranged by size:
5280 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2728 2763 529
Size: 35 2164 35 3860
Fragments arranged by size:
3860 2164 35 35
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 3271 3541
Size: 54 1425 50 275 18 412 1029 270
Cuts at: 3541 4430 6059 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 412 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3251 4024 1353
Size: 1898 773 3423
Fragments arranged by size:
3423 1898 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2692 2692
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2272 4157 4754 5541 5809 1239
Size: 393 500 140 1885 597 787 268 1524
Fragments arranged by size:
1885 1524 787 597 500 393 268 140
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 6094
EcoRI G'AATT_C
Cuts at: 2215 2389 2793 2877 3213 3403 3781 2215
Size: 174 404 84 336 190 378 4528
Fragments arranged by size:
4528 404 378 336 190 174 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2285 2521 2285
Size: 236 5858
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2287 5241 1678
Size: 609 2954 2531
Fragments arranged by size:
2954 2531 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 3180 3212 3250
Size: 32 135 32 560 32 1016 32 38
Cuts at: 3250 3282 4735 4767 4809 4841 1373
Size: 32 1453 32 42 32 2626
Fragments arranged by size:
2626 1453 1016 560 135 42 38 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2447 3438 3684 669
Size: 988 281 509 991 246 3079
Fragments arranged by size:
3079 991 988 509 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3097 3693 3097
Size: 596 5498
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4272
Fragments arranged by size:
4272 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 2634 2886 2970 3390 4052 4411
Size: 648 1300 168 252 84 420 662 359
Cuts at: 4411 4570 518
Size: 159 2042
Fragments arranged by size:
2042 1300 662 648 420 359 252 168
159 84
MspA1I CmG'CkG
Cuts at: 1606 3510 4187 5335 5580 1606
Size: 1904 677 1148 245 2120
Fragments arranged by size:
2120 1904 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4603
Fragments arranged by size:
4603 939 552
NdeI CA'TA_TG
Cuts at: 387 2155 387
Size: 1768 4326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 3348 3452 4997 1132
Size: 97 2119 104 1545 2229
Fragments arranged by size:
2229 2119 1545 104 97
PasI CC'CwG_GG
Cuts at: 1348 2011 1348
Size: 663 5431
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2017 1108
Size: 909 5185
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 6094
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspXI vC'TCGA_Gb
Cuts at: 2931 2931
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
SacI G_AGCT'C
Cuts at: 2694 2694
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SapI GCTCTTCn'nnn_
Cuts at: 3251 3251
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 2119 2175 3442 4577 5258 5449 826
Size: 219 1074 56 1267 1135 681 191 1471
Fragments arranged by size:
1471 1267 1135 1074 681 219 191 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 2969 1700
Size: 1269 4825
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2602 2931 4198 5099 5361 820
Size: 197 926 659 329 1267 901 262 1553
Fragments arranged by size:
1553 1267 926 901 659 329 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2420 2504 2672 2924 3008 3092 1517
Size: 903 84 168 252 84 84 4519
Fragments arranged by size:
4519 903 252 168 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2239 3533 513
Size: 552 939 235 1294 3074
Fragments arranged by size:
3074 1294 939 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3228 4121 4138 4291 4476 4921 325
Size: 1255 1648 893 17 153 185 445 1498
Fragments arranged by size:
1648 1498 1255 893 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3242 4340
Size: 274 80 33 51 1443 117 1148 1098
Cuts at: 4340 6052 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1148 1098 274 138 117 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 2931 1943
Size: 988 5106
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 2504 3092 3176 4223 2504
Size: 588 84 1047 4375
Fragments arranged by size:
4375 1047 588 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI