Restriction map for
pFN21AB4035
(Circular) (Six-base) MAPSORT of: pFN21AB4035.seq Check: 9829 from: 1 to: 6124
pFN21AB4035 6124 bp
With 182 enzymes: SgfI *
October 20, 2009 10:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 3467 1937
Size: 889 641 4594
Fragments arranged by size:
4594 889 641
Acc65I G'GTAC_C
Cuts at: 2963 3445 2963
Size: 482 5642
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3429 4187 5571 5839 1632
Size: 1797 758 1384 268 1917
Fragments arranged by size:
1917 1797 1384 758 268
AfeI AGC'GCT
Cuts at: 3025 3025
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 5023 6000 1203
Size: 3820 977 1327
Fragments arranged by size:
3820 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 2617 4853 2617
Size: 2236 3888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 2816 3047 3185 3867 5439 2816
Size: 231 138 682 1572 3501
Fragments arranged by size:
3501 1572 682 231 138
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2078 2737 3329 3433 3659 3811 5920 1493
Size: 585 659 592 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 659 592 585 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 2963 3445 4900 618
Size: 325 217 627 843 333 482 1455 1842
Fragments arranged by size:
1842 1455 843 627 482 333 325 217
BanII G_rGCy'C
Cuts at: 1391 3443 1391
Size: 2052 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 2139 2385 1548
Size: 591 246 5287
Fragments arranged by size:
5287 591 246
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 4735 136
Size: 107 122 71 631 605 614 2449 1525
Fragments arranged by size:
2449 1525 631 614 605 122 107 71
BglII A'GATC_T
Cuts at: 2764 3257 6119 2764
Size: 493 2862 2769
Fragments arranged by size:
2862 2769 493
BlpI GC'TnA_GC
Cuts at: 2108 3541 2108
Size: 1433 4691
Bme1580I G_kGCm'C
Cuts at: 1790 4185 5341 1790
Size: 2395 1156 2573
Fragments arranged by size:
2573 2395 1156
BmgBI CAC'GTC
Cuts at: 1318 2619 1318
Size: 1301 4823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 2504 2854
Size: 416 53 83 399 24 1024 63 350
Cuts at: 2854 2879 3855 4813 442
Size: 25 976 958 1753
Fragments arranged by size:
1753 1024 976 958 416 399 350 83
63 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4784 1239
Size: 3545 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 3295 4249 5114 5412 2189
Size: 1106 954 865 298 2901
Fragments arranged by size:
2901 1106 954 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2604 2689 3195 4787 882
Size: 617 1105 85 506 1592 2219
Fragments arranged by size:
2219 1592 1105 617 506 85
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 2376 2938 6118 2376
Size: 562 3180 2382
Fragments arranged by size:
3180 2382 562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3321 4557 4955 5229 5376 5787 3321
Size: 1236 398 274 147 411 3658
Fragments arranged by size:
3658 1236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2751 2781 1178
Size: 30 165 30 1348 30 4521
Fragments arranged by size:
4521 1348 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2715 3183 1186
Size: 1529 468 4127
Fragments arranged by size:
4127 1529 468
BseYI C'CCAG_C
Cuts at: 5327 5823 5327
Size: 496 5628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 2646 2658 3006 1163
Size: 573 865 45 12 348 4281
Fragments arranged by size:
4281 865 573 348 45 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 3574 4335 5363 1540
Size: 275 186 1309 264 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 264 186
BsiHKAI G_wGCw'C
Cuts at: 4185 4270 5341 4185
Size: 85 1071 4968
Fragments arranged by size:
4968 1071 85
BsiWI C'GTAC_G
Cuts at: 3294 3294
Size: 6124
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3443 4185 4270 5341 1391
Size: 399 1653 742 85 1071 2174
Fragments arranged by size:
2174 1653 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2573 2838 3479 844
Size: 1729 265 641 3489
Fragments arranged by size:
3489 1729 641 265
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2472 96
Size: 2376 3748
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 3245 4184 5196 3245
Size: 939 1012 4173
Fragments arranged by size:
4173 1012 939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 2764 3257 3454 3821 4206 4223 5675 5761
Size: 792 493 197 367 385 17 1452 86
Cuts at: 5761 5773 6119 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 792 493 385 367 346 197
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4434 4454 3640
Size: 794 20 5310
Fragments arranged by size:
5310 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089
Size: 54 1425 50 275 18 1741 889 1629
Cuts at: 6089 8
Size: 43
Fragments arranged by size:
1741 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3033 4054 1353
Size: 1680 1021 3423
Fragments arranged by size:
3423 1680 1021
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3429 4187 4784 5571 5839 1239
Size: 393 1797 758 597 787 268 1524
Fragments arranged by size:
1797 1524 787 758 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 6124
EcoRI G'AATT_C
Cuts at: 3329 3433 3811 3329
Size: 104 378 5642
Fragments arranged by size:
5642 378 104
EcoRV GAT'ATC
Cuts at: 2936 2936
Size: 6124
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 3027 5271 1678
Size: 1349 2244 2531
Fragments arranged by size:
2531 2244 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 2981 3013 3124
Size: 32 135 32 1031 32 346 32 111
Cuts at: 3124 3156 4765 4797 4839 4871 1373
Size: 32 1609 32 42 32 2626
Fragments arranged by size:
2626 1609 1031 346 135 111 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3268 3468 3714 669
Size: 988 281 889 441 200 246 3079
Fragments arranged by size:
3079 988 889 441 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 2967 3449 2967
Size: 482 5642
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4302
Fragments arranged by size:
4302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2447 4082 4441 4600 518
Size: 648 1020 261 1635 359 159 2042
Fragments arranged by size:
2042 1635 1020 648 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 3282 3540 4217 5365 5610 1606
Size: 1676 258 677 1148 245 2120
Fragments arranged by size:
2120 1676 1148 677 258 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
NdeI CA'TA_TG
Cuts at: 387 2959 387
Size: 2572 3552
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 3079 3400 3482 5027 1132
Size: 97 1850 321 82 1545 2229
Fragments arranged by size:
2229 1850 1545 321 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2538 3476 830
Size: 1708 938 3478
Fragments arranged by size:
3478 1708 938
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 3282 3282
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 2825 3466 1936
Size: 889 641 4594
Fragments arranged by size:
4594 889 641
SapI GCTCTTCn'nnn_
Cuts at: 3033 3033
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 2972 4372 6084 2972
Size: 1400 1712 3012
Fragments arranged by size:
3012 1712 1400
SexAI A'CCwGG_T
Cuts at: 2671 2689 2671
Size: 18 6106
SfcI C'TryA_G
Cuts at: 826 1045 2534 3283 3472 4607 5288 5479 826
Size: 219 1489 749 189 1135 681 191 1471
Fragments arranged by size:
1489 1471 1135 749 681 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 3274 4228 5129 5391 820
Size: 197 926 261 1070 954 901 262 1553
Fragments arranged by size:
1553 1070 954 926 901 262 261 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3400 3482 1132
Size: 2268 82 3774
Fragments arranged by size:
3774 2268 82
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 2004 3034 3563 513
Size: 552 939 1030 529 3074
Fragments arranged by size:
3074 1030 939 552 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3324 4151 4168 4321 4506 4951
Size: 1255 1014 730 827 17 153 185 445
Cuts at: 4951 325
Size: 1498
Fragments arranged by size:
1498 1255 1014 827 730 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2217 2352
Size: 274 80 33 51 1443 231 9 135
Cuts at: 2352 2472 2970 4370 6082 96
Size: 120 498 1400 1712 138
Fragments arranged by size:
1712 1443 1400 498 274 231 138 135
120 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6084 6116 6084
Size: 32 6092
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 3341 2185
Size: 1156 4968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 4253 3329
Size: 924 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI