Restriction map for
pFN21AB4033
(Circular) (Six-base) MAPSORT of: pFN21AB4033.seq Check: 7452 from: 1 to: 8491
pFN21AB4033 8491 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3948 4748 6301 278
Size: 53 83 186 936 2412 800 1553 2468
Fragments arranged by size:
2468 2412 1553 936 800 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2402 4649 5834 1937
Size: 465 2247 1185 4594
Fragments arranged by size:
4594 2247 1185 465
Acc65I G'GTAC_C
Cuts at: 5812 5812
Size: 8491
AclI AA'CG_TT
Cuts at: 1563 4002 6618 6991 1563
Size: 2439 2616 373 3063
Fragments arranged by size:
3063 2616 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2601 2915 3051 3588 4739 5076 5366
Size: 432 537 314 136 537 1151 337 290
Cuts at: 5366 6554 7938 8206 1632
Size: 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1188 1151 537 537 432 337
314 290 268 136
AflII C'TTAA_G
Cuts at: 820 1017 3950 820
Size: 197 2933 5361
Fragments arranged by size:
5361 2933 197
AflIII A'CryG_T
Cuts at: 1203 7390 8367 1203
Size: 6187 977 1327
Fragments arranged by size:
6187 1327 977
AgeI A'CCGG_T
Cuts at: 4083 7322 4083
Size: 3239 5252
AhdI GACnn_n'nnGTC
Cuts at: 7220 7220
Size: 8491
AleI CACnn'nnGTG
Cuts at: 1166 2226 5557 1166
Size: 1060 3331 4100
Fragments arranged by size:
4100 3331 1060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5603 1184
Size: 4419 4072
AlwNI CAG_nnn'CTG
Cuts at: 3390 5539 6234 7806 3390
Size: 2149 695 1572 4075
Fragments arranged by size:
4075 2149 1572 695
ApaLI G'TGCA_C
Cuts at: 6548 7704 6548
Size: 1156 7335
ApoI r'AATT_y
Cuts at: 1493 2032 2075 2123 4034 5800 6026 6178 8287
Size: 539 43 48 1911 1766 226 152 2109
Cuts at: 8287 1493
Size: 1697
Fragments arranged by size:
2109 1911 1766 1697 539 226 152 48
43
AseI AT'TA_AT
Cuts at: 160 1032 7045 160
Size: 872 6013 1606
Fragments arranged by size:
6013 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8491
AvaI C'yCGr_G
Cuts at: 1870 1943 4965 5816 1870
Size: 73 3022 851 4545
Fragments arranged by size:
4545 3022 851 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3115 3148 3115
Size: 33 8458
BamHI G'GATC_C
Cuts at: 3548 4089 5821 6188 3548
Size: 541 1732 367 5851
Fragments arranged by size:
5851 1732 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2846 3395 4162 5812 7267
Size: 325 217 627 1059 549 767 1650 1455
Cuts at: 7267 618
Size: 1842
Fragments arranged by size:
1842 1650 1455 1059 767 627 549 325
217
BanII G_rGCy'C
Cuts at: 1391 5810 1391
Size: 4419 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2482 4796 4839 5389 5624 5727 5741
Size: 971 583 2314 43 550 235 103 14
Cuts at: 5741 5756 928
Size: 15 3663
Fragments arranged by size:
3663 2314 971 583 550 235 103 43
15 14
BbvCI CC'TCA_GC
Cuts at: 2994 2994
Size: 8491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6682 6716 1903
Size: 34 50 34 4661 34 3678
Fragments arranged by size:
4661 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2469 4548 6383 7593 2469
Size: 2079 1835 1210 3367
Fragments arranged by size:
3367 2079 1835 1210
BclI T'GATC_A
Cuts at: 1548 4506 4858 5490 1548
Size: 2958 352 632 4549
Fragments arranged by size:
4549 2958 632 352
BfrBI ATG'CAT
Cuts at: 3444 3444
Size: 8491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4733 7102 136
Size: 107 122 71 631 605 3061 2369 1525
Fragments arranged by size:
3061 2369 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3486 3493 8486 3486
Size: 7 4993 3491
Fragments arranged by size:
4993 3491 7
BlpI GC'TnA_GC
Cuts at: 5908 5908
Size: 8491
Bme1580I G_kGCm'C
Cuts at: 1790 3243 3398 5582 6552 7708 1790
Size: 1453 155 2184 970 1156 2573
Fragments arranged by size:
2573 2184 1453 1156 970 155
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8491
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2312 3316 3400
Size: 416 53 83 399 24 895 1004 84
Cuts at: 3400 4803 6222 7180 442
Size: 1403 1419 958 1753
Fragments arranged by size:
1753 1419 1403 1004 958 895 416 399
84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5315 5315
Size: 8491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3226 3998 4796 7151 1239
Size: 1987 772 798 2355 2579
Fragments arranged by size:
2579 2355 1987 798 772
Bpu10I CC'TnA_GC
Cuts at: 2994 3612 4679 4905 2994
Size: 618 1067 226 6580
Fragments arranged by size:
6580 1067 618 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6616 7481 7779 6616
Size: 865 298 7328
Fragments arranged by size:
7328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4506 5690 7154 882
Size: 617 3007 1184 1464 2219
Fragments arranged by size:
3007 2219 1464 1184 617
BsaAI yAC'GTr
Cuts at: 493 1204 3362 6282 493
Size: 711 2158 2920 2702
Fragments arranged by size:
2920 2702 2158 711
BsaBI GATnn'nnATC
Cuts at: 8485 8485
Size: 8491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3945 4745 6298 6680
Size: 53 83 186 936 2412 800 1553 382
Cuts at: 6680 7315 275
Size: 635 1451
Fragments arranged by size:
2412 1553 1451 936 800 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4083 6924 7322 7596 7743 8154 4083
Size: 2841 398 274 147 411 4420
Fragments arranged by size:
4420 2841 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2665 2695 1178
Size: 30 165 30 1262 30 6974
Fragments arranged by size:
6974 1262 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3210 3446 4784 1186
Size: 2024 236 1338 4893
Fragments arranged by size:
4893 2024 1338 236
BseYI C'CCAG_C
Cuts at: 3242 7694 8190 3242
Size: 4452 496 3543
Fragments arranged by size:
4452 3543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4485 1163
Size: 573 2749 5169
Fragments arranged by size:
5169 2749 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5941 6702 7730 1540
Size: 275 186 3940 761 1028 2301
Fragments arranged by size:
3940 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6552 6637 7708 6552
Size: 85 1071 7335
Fragments arranged by size:
7335 1071 85
BsmI GAATG_Cn'
Cuts at: 2099 4404 4671 5548 6002 6095 2099
Size: 2305 267 877 454 93 4495
Fragments arranged by size:
4495 2305 877 454 267 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3243 3398 5582 5810 6552 6637 7708
Size: 399 1453 155 2184 228 742 85 1071
Cuts at: 7708 1391
Size: 2174
Fragments arranged by size:
2184 2174 1453 1071 742 399 228 155
85
BspEI T'CCGG_A
Cuts at: 8154 8154
Size: 8491
BspHI T'CATG_A
Cuts at: 6380 8110 6380
Size: 1730 6761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5846 844
Size: 5002 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6378 1394
Size: 429 4555 3507
Fragments arranged by size:
4555 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3672 6986 7160 65
Size: 1183 2424 3314 174 1396
Fragments arranged by size:
3314 2424 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4083 7135 7322 1700
Size: 110 2273 3052 187 2869
Fragments arranged by size:
3052 2869 2273 187 110
BsrGI T'GTAC_A
Cuts at: 96 4848 96
Size: 4752 3739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8491
BssSI C'ACGA_G
Cuts at: 6551 7563 6551
Size: 1012 7479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5004 794
Size: 4210 4281
BstBI TT'CG_AA
Cuts at: 4633 7295 4633
Size: 2662 5829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8491
BstXI CCAn_nnnn'nTGG
Cuts at: 3857 4793 4807 3857
Size: 936 14 7541
Fragments arranged by size:
7541 936 14
BstYI r'GATC_y
Cuts at: 1972 2611 2826 3486 3493 3548 3832 4089 4570
Size: 639 215 660 7 55 284 257 481
Cuts at: 4570 5189 5219 5821 6188 6573 6590 8042 8128
Size: 619 30 602 367 385 17 1452 86
Cuts at: 8128 8140 8486 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 660 639 619 602 481 385
367 346 284 257 215 86 55 30
17 12 7
Bsu36I CC'TnA_GG
Cuts at: 3284 3284
Size: 8491
BtgI C'CryG_G
Cuts at: 513 1065 2266 3729 3968 513
Size: 552 1201 1463 239 5036
Fragments arranged by size:
5036 1463 1201 552 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3435 487
Size: 683 56 2209 5543
Fragments arranged by size:
5543 2209 683 56
BtsI GCAGTG_nn'
Cuts at: 3243 4743 4771 5284 6007 6801 6821 3243
Size: 1500 28 513 723 794 20 4913
Fragments arranged by size:
4913 1500 794 723 513 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8456
DraI TTT'AAA
Cuts at: 3925 5340 5795 3925
Size: 1415 455 6621
Fragments arranged by size:
6621 1415 455
DraIII CAC_nnn'GTG
Cuts at: 3539 5462 3539
Size: 1923 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3005 7498 809
Size: 507 1689 4493 1802
Fragments arranged by size:
4493 1802 1689 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2419 3726
Size: 54 1425 50 275 18 498 91 1307
Cuts at: 3726 3796 5938 6827 8456 8
Size: 70 2142 889 1629 43
Fragments arranged by size:
2142 1629 1425 1307 889 498 275 91
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5938 1537
Size: 275 4126 4090
Fragments arranged by size:
4126 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 3924 4495 5342 5514 6421 1353
Size: 690 1881 571 847 172 907 3423
Fragments arranged by size:
3423 1881 907 847 690 571 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3473 4829 7078 7452 7598 387
Size: 1064 138 1884 1356 2249 374 146 1280
Fragments arranged by size:
2249 1884 1356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2601 2915 3051 3226 3588 3998
Size: 393 432 537 314 136 175 362 410
Cuts at: 3998 4739 4796 5076 5366 6554 7151 7938 8206
Size: 741 57 280 290 1188 597 787 268
Cuts at: 8206 1239
Size: 1524
Fragments arranged by size:
1524 1188 787 741 597 537 432 410
393 362 314 290 280 268 175 136
57
EcoO109I rG'GnC_Cy
Cuts at: 2874 3310 2874
Size: 436 8055
EcoRI G'AATT_C
Cuts at: 5800 6178 5800
Size: 378 8113
EcoRV GAT'ATC
Cuts at: 4591 4591
Size: 8491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3179 3568 4744 5140 3179
Size: 389 1176 396 6530
Fragments arranged by size:
6530 1176 396 389
FspI TGC'GCA
Cuts at: 4881 6997 4881
Size: 2116 6375
HaeII r_GCGC'y
Cuts at: 1678 2916 7638 1678
Size: 1238 4722 2531
Fragments arranged by size:
4722 2531 1238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2665 2697 2754
Size: 32 135 32 812 32 249 32 57
Cuts at: 2754 2786 3471 3478 3503 3510 4580 4612 7132
Size: 32 685 7 25 7 1070 32 2520
Cuts at: 7132 7164 7206 7238 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2520 1070 812 685 249 135 57
42 32 32 32 32 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 2932 3514 5835 6081 669
Size: 988 281 304 690 582 2321 246 3079
Fragments arranged by size:
3079 2321 988 690 582 304 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8491
HpaI GTT'AAC
Cuts at: 6081 6081
Size: 8491
KpnI G_GTAC'C
Cuts at: 5816 5816
Size: 8491
MfeI C'AATT_G
Cuts at: 2370 4198 6090 2370
Size: 1828 1892 4771
Fragments arranged by size:
4771 1892 1828
MluI A'CGCG_T
Cuts at: 8367 8367
Size: 8491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 5042 5310 7605 7789 1305
Size: 1293 2444 268 2295 184 2007
Fragments arranged by size:
2444 2295 2007 1293 268 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 3728 3798 10
Size: 54 1425 343 498 1398 70 4703
Fragments arranged by size:
4703 1425 1398 498 343 70 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2777 4805 4899 5557 5729 6449
Size: 648 1060 551 2028 94 658 172 720
Cuts at: 6449 6808 6967 518
Size: 359 159 2042
Fragments arranged by size:
2042 2028 1060 720 658 648 551 359
172 159 94
MspA1I CmG'CkG
Cuts at: 1606 3246 5907 6584 7732 7977 1606
Size: 1640 2661 677 1148 245 2120
Fragments arranged by size:
2661 2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2266 3968 513
Size: 552 1201 1702 5036
Fragments arranged by size:
5036 1702 1201 552
NdeI CA'TA_TG
Cuts at: 387 5248 387
Size: 4861 3630
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8491
NotI GC'GGCC_GC
Cuts at: 5938 5938
Size: 8491
NsiI A_TGCA'T
Cuts at: 3446 3446
Size: 8491
NspI r_CATG'y
Cuts at: 1132 1229 2559 3604 5849 7394 1132
Size: 97 1330 1045 2245 1545 2229
Fragments arranged by size:
2245 2229 1545 1330 1045 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8491
PciI A'CATG_T
Cuts at: 7390 7390
Size: 8491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2685 3702 4945 1108
Size: 1577 1017 1243 4654
Fragments arranged by size:
4654 1577 1243 1017
PfoI T'CCnGG_A
Cuts at: 5301 5301
Size: 8491
PmeI GTTT'AAAC
Cuts at: 5795 5795
Size: 8491
PmlI CAC'GTG
Cuts at: 3362 3362
Size: 8491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6560 6592 8073 8105 6560
Size: 32 1481 32 6946
Fragments arranged by size:
6946 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3310 3310
Size: 8491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8491
PsiI TTA'TAA
Cuts at: 2598 6061 2598
Size: 3463 5028
PstI C_TGCA'G
Cuts at: 830 3667 5473 5843 830
Size: 2837 1806 370 3478
Fragments arranged by size:
3478 2837 1806 370
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8491
PvuII CAG'CTG
Cuts at: 3246 3246
Size: 8491
SalI G'TCGA_C
Cuts at: 1936 5833 1936
Size: 3897 4594
SanDI GG'GwC_CC
Cuts at: 3310 3310
Size: 8491
SbfI CC_TGCA'GG
Cuts at: 5843 5843
Size: 8491
ScaI AGT'ACT
Cuts at: 5506 6739 8451 5506
Size: 1233 1712 5546
Fragments arranged by size:
5546 1712 1233
SfcI C'TryA_G
Cuts at: 826 1045 3663 4650 5469 5531 5839 6974 7655
Size: 219 2618 987 819 62 308 1135 681
Cuts at: 7655 7846 826
Size: 191 1471
Fragments arranged by size:
2618 1471 1135 987 819 681 308 219
191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8491
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8491
SmaI CCC'GGG
Cuts at: 1872 5818 1872
Size: 3946 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3950 6595 7496 7758 820
Size: 197 926 2007 2645 901 262 1553
Fragments arranged by size:
2645 2007 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6282 493
Size: 5789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8491
SphI G_CATG'C
Cuts at: 1132 3604 5849 1132
Size: 2472 2245 3774
Fragments arranged by size:
3774 2472 2245
SspI AAT'ATT
Cuts at: 5 52 6415 5
Size: 47 6363 2081
Fragments arranged by size:
6363 2081 47
StuI AGG'CCT
Cuts at: 1517 2514 1517
Size: 997 7494
StyI C'CwwG_G
Cuts at: 513 1065 2266 3968 5626 5649 5930 513
Size: 552 1201 1702 1658 23 281 3074
Fragments arranged by size:
3074 1702 1658 1201 552 281 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4530 5636 6518 6535 6688 6873 7318
Size: 1255 2950 1106 882 17 153 185 445
Cuts at: 7318 325
Size: 1498
Fragments arranged by size:
2950 1498 1255 1106 882 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2868 3958 4848
Size: 274 80 33 51 1443 891 1090 890
Cuts at: 4848 5037 5504 6737 8449 96
Size: 189 467 1233 1712 138
Fragments arranged by size:
1712 1443 1233 1090 891 890 467 274
189 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3096 3128 8451 8483 3096
Size: 32 5323 32 3104
Fragments arranged by size:
5323 3104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8491
XbaI T'CTAG_A
Cuts at: 5827 5827
Size: 8491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8491
XmaI C'CCGG_G
Cuts at: 1870 5816 1870
Size: 3946 4545
XmnI GAAnn'nnTTC
Cuts at: 2838 6620 2838
Size: 3782 4709
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3946 4746 6299 276
Size: 53 83 186 936 2412 800 1553 2468
Fragments arranged by size:
2468 2412 1553 936 800 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI