Restriction map for
pFN21AB4032
(Circular) (Six-base) MAPSORT of: pFN21AB4032.seq Check: 7313 from: 1 to: 8107
pFN21AB4032 8107 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3763 3763
Size: 8107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5917 278
Size: 53 83 186 936 4381 2468
Fragments arranged by size:
4381 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 3411 3860 5450 1937
Size: 1146 328 449 1590 4594
Fragments arranged by size:
4594 1590 1146 449 328
Acc65I G'GTAC_C
Cuts at: 5428 5428
Size: 8107
AclI AA'CG_TT
Cuts at: 1563 6234 6607 1563
Size: 4671 373 3063
Fragments arranged by size:
4671 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2399 3147 3570 3799 4053 4680 6170
Size: 549 218 748 423 229 254 627 1490
Cuts at: 6170 7554 7822 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1490 1384 748 627 549 423 268
254 229 218
AfeI AGC'GCT
Cuts at: 2486 2486
Size: 8107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7910
AflIII A'CryG_T
Cuts at: 1203 4399 4591 7006 7983 1203
Size: 3196 192 2415 977 1327
Fragments arranged by size:
3196 2415 1327 977 192
AgeI A'CCGG_T
Cuts at: 6938 6938
Size: 8107
AhdI GACnn_n'nnGTC
Cuts at: 2791 6836 2791
Size: 4045 4062
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2547 5124 5134 1184
Size: 1363 2577 10 4157
Fragments arranged by size:
4157 2577 1363 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 8075
AlwNI CAG_nnn'CTG
Cuts at: 2804 3190 4049 4061 4349 5850 7422 2804
Size: 386 859 12 288 1501 1572 3489
Fragments arranged by size:
3489 1572 1501 859 386 288 12
ApaI G_GGCC'C
Cuts at: 4668 4668
Size: 8107
ApaLI G'TGCA_C
Cuts at: 6164 7320 6164
Size: 1156 6951
ApoI r'AATT_y
Cuts at: 1493 4098 4790 4835 5416 5642 5794 7903 1493
Size: 2605 692 45 581 226 152 2109 1697
Fragments arranged by size:
2605 2109 1697 692 581 226 152 45
AscI GG'CGCG_CC
Cuts at: 5321 5321
Size: 8107
AseI AT'TA_AT
Cuts at: 160 1032 3549 6661 160
Size: 872 2517 3112 1606
Fragments arranged by size:
3112 2517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
AvaI C'yCGr_G
Cuts at: 1870 1943 5432 1870
Size: 73 3489 4545
Fragments arranged by size:
4545 3489 73
AvrII C'CTAG_G
Cuts at: 2387 2387
Size: 8107
BamHI G'GATC_C
Cuts at: 2314 5437 5804 2314
Size: 3123 367 4617
Fragments arranged by size:
4617 3123 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3874 5428 6883 618
Size: 325 217 627 2087 1554 1455 1842
Fragments arranged by size:
2087 1842 1554 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2706 4410 4668 5426 1391
Size: 1315 1704 258 758 4072
Fragments arranged by size:
4072 1704 1315 758 258
BbeI G_GCGC'C
Cuts at: 3878 3878
Size: 8107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4680 5298 928
Size: 971 2781 618 3737
Fragments arranged by size:
3737 2781 971 618
BbvCI CC'TCA_GC
Cuts at: 2945 3362 2945
Size: 417 7690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3664 3698 6298 6332 1903
Size: 34 50 34 1643 34 2600 34 3678
Fragments arranged by size:
3678 2600 1643 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5999 7209 5999
Size: 1210 6897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2280 3106 4663
Size: 107 122 71 631 605 608 826 1557
Cuts at: 4663 6718 136
Size: 2055 1525
Fragments arranged by size:
2055 1557 1525 826 631 608 605 122
107 71
BglII A'GATC_T
Cuts at: 2306 3203 3277 8102 2306
Size: 897 74 4825 2311
Fragments arranged by size:
4825 2311 897 74
BlpI GC'TnA_GC
Cuts at: 2535 2586 2805 5524 2535
Size: 51 219 2719 5118
Fragments arranged by size:
5118 2719 219 51
Bme1580I G_kGCm'C
Cuts at: 1790 4668 6168 7324 1790
Size: 2878 1500 1156 2573
Fragments arranged by size:
2878 2573 1500 1156
BmgBI CAC'GTC
Cuts at: 1318 2113 1318
Size: 795 7312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4367 5838
Size: 416 53 83 399 24 1913 1037 1471
Cuts at: 5838 6796 442
Size: 958 1753
Fragments arranged by size:
1913 1753 1471 1037 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4333 4441 4333
Size: 108 7999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 3714 4488 4538 4631 6767 1239
Size: 837 1638 774 50 93 2136 2579
Fragments arranged by size:
2579 2136 1638 837 774 93 50
Bpu10I CC'TnA_GC
Cuts at: 2945 3362 3486 2945
Size: 417 124 7566
Fragments arranged by size:
7566 417 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3088 6232 7097 7395 2741
Size: 347 3144 865 298 3453
Fragments arranged by size:
3453 3144 865 347 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 4429 4720 6770 882
Size: 617 2805 125 291 2050 2219
Fragments arranged by size:
2805 2219 2050 617 291 125
BsaAI yAC'GTr
Cuts at: 493 1204 2620 5354 5898 493
Size: 711 1416 2734 544 2702
Fragments arranged by size:
2734 2702 1416 711 544
BsaBI GATnn'nnATC
Cuts at: 3015 8101 3015
Size: 5086 3021
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3875 5914 6296 6931
Size: 53 83 186 936 2342 2039 382 635
Cuts at: 6931 275
Size: 1451
Fragments arranged by size:
2342 2039 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6540 6938 7212 7359 7770 6540
Size: 398 274 147 411 6877
Fragments arranged by size:
6877 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3712 3742 5058 5088 1178
Size: 30 165 30 2309 30 1316 30 4197
Fragments arranged by size:
4197 2309 1316 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4628 1186
Size: 3442 4665
BseYI C'CCAG_C
Cuts at: 2454 3024 3692 3783 5362 7310 7806 2454
Size: 570 668 91 1579 1948 496 2755
Fragments arranged by size:
2755 1948 1579 668 570 496 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3923 4947 1163
Size: 573 2187 1024 4323
Fragments arranged by size:
4323 2187 1024 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5557 6318 7346 1540
Size: 275 186 3556 761 1028 2301
Fragments arranged by size:
3556 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2608 2706 5153 6168 6253 7324 2608
Size: 98 2447 1015 85 1071 3391
Fragments arranged by size:
3391 2447 1071 1015 98 85
BsmI GAATG_Cn'
Cuts at: 2869 3331 4471 5618 5711 2869
Size: 462 1140 1147 93 5265
Fragments arranged by size:
5265 1147 1140 462 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2103
Size: 8107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2608 2706 4410 4668 5153 5426 6168
Size: 399 818 98 1704 258 485 273 742
Cuts at: 6168 6253 7324 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1704 1071 818 742 485 399 273
258 98 85
BspEI T'CCGG_A
Cuts at: 7770 7770
Size: 8107
BspHI T'CATG_A
Cuts at: 2266 5996 7726 2266
Size: 3730 1730 2647
Fragments arranged by size:
3730 2647 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3763 4702 5462 844
Size: 2919 939 760 3489
Fragments arranged by size:
3489 2919 939 760
BsrBI CCG'CTC
Cuts at: 1394 1823 5994 1394
Size: 429 4171 3507
Fragments arranged by size:
4171 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2647 6602 6776 65
Size: 1183 1399 3955 174 1396
Fragments arranged by size:
3955 1399 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2952 6751 6938 1700
Size: 110 1142 3799 187 2869
Fragments arranged by size:
3799 2869 1142 187 110
BsrGI T'GTAC_A
Cuts at: 96 4846 96
Size: 4750 3357
BssHII G'CGCG_C
Cuts at: 1925 5321 1925
Size: 3396 4711
BssSI C'ACGA_G
Cuts at: 6167 7179 6167
Size: 1012 7095
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2464 3901 794
Size: 1670 1437 5000
Fragments arranged by size:
5000 1670 1437
BstBI TT'CG_AA
Cuts at: 6911 6911
Size: 8107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8107
BstXI CCAn_nnnn'nTGG
Cuts at: 3248 3248
Size: 8107
BstYI r'GATC_y
Cuts at: 1972 2119 2306 2314 2395 3203 3277 5437 5804
Size: 147 187 8 81 808 74 2160 367
Cuts at: 5804 6189 6206 7658 7744 7756 8102 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2160 1977 1452 808 385 367 346 187
147 86 81 74 17 12 8
BstZ17I GTA'TAC
Cuts at: 3861 3861
Size: 8107
Bsu36I CC'TnA_GG
Cuts at: 4524 4524
Size: 8107
BtgI C'CryG_G
Cuts at: 513 1065 3639 3831 4305 513
Size: 552 2574 192 474 4315
Fragments arranged by size:
4315 2574 552 474 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7368
Fragments arranged by size:
7368 683 56
BtsI GCAGTG_nn'
Cuts at: 2805 3188 3469 5623 6417 6437 2805
Size: 383 281 2154 794 20 4475
Fragments arranged by size:
4475 2154 794 383 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3033 3068 5340 5375 529
Size: 35 2469 35 2272 35 3261
Fragments arranged by size:
3261 2469 2272 35 35 35
DraI TTT'AAA
Cuts at: 2325 5411 2325
Size: 3086 5021
DraIII CAC_nnn'GTG
Cuts at: 5148 5148
Size: 8107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7114 809
Size: 507 5798 1802
Fragments arranged by size:
5798 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3709 4106 4302
Size: 54 1425 50 275 18 1879 397 196
Cuts at: 4302 5403 5554 6443 8072 8
Size: 1101 151 889 1629 43
Fragments arranged by size:
1879 1629 1425 1101 889 397 275 196
151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5554 1537
Size: 275 3742 4090
Fragments arranged by size:
4090 3742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 4914 6037 1353
Size: 1271 2290 1123 3423
Fragments arranged by size:
3423 2290 1271 1123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6694 7068 7214 387
Size: 1064 138 5105 374 146 1280
Fragments arranged by size:
5105 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2704 2704
Size: 8107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2181 2399 3147 3570 3714 3799
Size: 393 444 105 218 748 423 144 85
Cuts at: 3799 4053 4488 4538 4631 4680 6170 6767 7554
Size: 254 435 50 93 49 1490 597 787
Cuts at: 7554 7822 1239
Size: 268 1524
Fragments arranged by size:
1524 1490 787 748 597 444 435 423
393 268 254 218 144 105 93 85
50 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 4503 2822
Size: 1681 6426
EcoO109I rG'GnC_Cy
Cuts at: 3065 4250 4664 3065
Size: 1185 414 6508
Fragments arranged by size:
6508 1185 414
EcoRI G'AATT_C
Cuts at: 4098 5416 5794 4098
Size: 1318 378 6411
Fragments arranged by size:
6411 1318 378
EcoRV GAT'ATC
Cuts at: 3472 3472
Size: 8107
FspI TGC'GCA
Cuts at: 6613 6613
Size: 8107
HaeII r_GCGC'y
Cuts at: 1678 2488 3878 7254 1678
Size: 810 1390 3376 2531
Fragments arranged by size:
3376 2531 1390 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 3712 3744 3907
Size: 32 135 32 1205 32 903 32 163
Cuts at: 3907 3939 6748 6780 6822 6854 1373
Size: 32 2809 32 42 32 2626
Fragments arranged by size:
2809 2626 1205 903 163 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3624 3915 5451 5697 669
Size: 988 281 1686 291 1536 246 3079
Fragments arranged by size:
3079 1686 1536 988 291 281 246
HindIII A'AGCT_T
Cuts at: 748 4274 748
Size: 3526 4581
HpaI GTT'AAC
Cuts at: 5697 5697
Size: 8107
KasI G'GCGC_C
Cuts at: 3874 3874
Size: 8107
KpnI G_GTAC'C
Cuts at: 5432 5432
Size: 8107
MfeI C'AATT_G
Cuts at: 2462 5706 2462
Size: 3244 4863
MluI A'CGCG_T
Cuts at: 7983 7983
Size: 8107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7221 7405 1305
Size: 5916 184 2007
Fragments arranged by size:
5916 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3711 10
Size: 54 1425 343 1879 4406
Fragments arranged by size:
4406 1879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6065 6424 6583 518
Size: 648 4899 359 159 2042
Fragments arranged by size:
4899 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3361 5072 5523 6200 7348 7593 1606
Size: 1755 1711 451 677 1148 245 2120
Fragments arranged by size:
2120 1755 1711 1148 677 451 245
NarI GG'CG_CC
Cuts at: 3875 3875
Size: 8107
NcoI C'CATG_G
Cuts at: 513 1065 3639 4305 513
Size: 552 2574 666 4315
Fragments arranged by size:
4315 2574 666 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8107
NotI GC'GGCC_GC
Cuts at: 5554 5554
Size: 8107
NspI r_CATG'y
Cuts at: 1132 1229 4403 4492 4595 4690 5101 5465 7010
Size: 97 3174 89 103 95 411 364 1545
Cuts at: 7010 1132
Size: 2229
Fragments arranged by size:
3174 2229 1545 411 364 103 97 95
89
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8107
PciI A'CATG_T
Cuts at: 4399 4591 7006 4399
Size: 192 2415 5500
Fragments arranged by size:
5500 2415 192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3832 1108
Size: 2724 5383
PmeI GTTT'AAAC
Cuts at: 5411 5411
Size: 8107
PmlI CAC'GTG
Cuts at: 2620 2620
Size: 8107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6176 6208 7689 7721 6176
Size: 32 1481 32 6562
Fragments arranged by size:
6562 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3065 4250 3065
Size: 1185 6922
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8107
PsiI TTA'TAA
Cuts at: 5677 5677
Size: 8107
PspOMI G'GGCC_C
Cuts at: 4664 4664
Size: 8107
PstI C_TGCA'G
Cuts at: 830 2800 2835 3061 5113 5459 830
Size: 1970 35 226 2052 346 3478
Fragments arranged by size:
3478 2052 1970 346 226 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8107
SacI G_AGCT'C
Cuts at: 2706 2706
Size: 8107
SalI G'TCGA_C
Cuts at: 1936 5449 1936
Size: 3513 4594
SapI GCTCTTCn'nnn_
Cuts at: 4914 4914
Size: 8107
SbfI CC_TGCA'GG
Cuts at: 5459 5459
Size: 8107
ScaI AGT'ACT
Cuts at: 6355 8067 6355
Size: 1712 6395
SfcI C'TryA_G
Cuts at: 826 1045 2796 2831 2978 3057 5089 5109 5455
Size: 219 1751 35 147 79 2032 20 346
Cuts at: 5455 6590 7271 7462 826
Size: 1135 681 191 1471
Fragments arranged by size:
2032 1751 1471 1135 681 346 219 191
147 79 35 20
SfoI GGC'GCC
Cuts at: 3876 3876
Size: 8107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8107
SmaI CCC'GGG
Cuts at: 1872 5434 1872
Size: 3562 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 3103 6211 7112 7374 820
Size: 197 926 813 347 3108 901 262 1553
Fragments arranged by size:
3108 1553 926 901 813 347 262 197
SnaBI TAC'GTA
Cuts at: 493 5898 493
Size: 5405 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8107
SphI G_CATG'C
Cuts at: 1132 4690 5465 1132
Size: 3558 775 3774
Fragments arranged by size:
3774 3558 775
SspI AAT'ATT
Cuts at: 5 52 4267 6031 5
Size: 47 4215 1764 2081
Fragments arranged by size:
4215 2081 1764 47
StuI AGG'CCT
Cuts at: 1517 3109 4213 4509 1517
Size: 1592 1104 296 5115
Fragments arranged by size:
5115 1592 1104 296
StyI C'CwwG_G
Cuts at: 513 1065 2387 3147 3292 3639 4208 4305 5546
Size: 552 1322 760 145 347 569 97 1241
Cuts at: 5546 513
Size: 3074
Fragments arranged by size:
3074 1322 1241 760 569 552 347 145
97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4014 6134 6151 6304 6489 6934 325
Size: 1255 2434 2120 17 153 185 445 1498
Fragments arranged by size:
2434 2120 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4472 4846 6353
Size: 274 80 33 51 1443 2495 374 1507
Cuts at: 6353 8065 96
Size: 1712 138
Fragments arranged by size:
2495 1712 1507 1443 374 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 2942 2974 3790 3822 4770 4802 5058
Size: 32 845 32 816 32 948 32 256
Cuts at: 5058 5090 8067 8099 2065
Size: 32 2977 32 2073
Fragments arranged by size:
2977 2073 948 845 816 256 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8107
XbaI T'CTAG_A
Cuts at: 5443 5443
Size: 8107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4031 4303 5162 4031
Size: 272 859 6976
Fragments arranged by size:
6976 859 272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8107
XmaI C'CCGG_G
Cuts at: 1870 5432 1870
Size: 3562 4545
XmnI GAAnn'nnTTC
Cuts at: 4098 6236 4098
Size: 2138 5969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5915 276
Size: 53 83 186 936 4381 2468
Fragments arranged by size:
4381 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
BaeI BfrBI BsiWI FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SrfI SwaI