Restriction map for
pFN21AB4000
(Circular) (Six-base) MAPSORT of: pFN21AB4000.seq Check: 3305 from: 1 to: 5347
pFN21AB4000 5347 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3157 278
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2690 1937
Size: 753 4594
Acc65I G'GTAC_C
Cuts at: 2668 2668
Size: 5347
AclI AA'CG_TT
Cuts at: 1563 3474 3847 1563
Size: 1911 373 3063
Fragments arranged by size:
3063 1911 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3410 4794 5062 1632
Size: 1778 1384 268 1917
Fragments arranged by size:
1917 1778 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5150
AflIII A'CryG_T
Cuts at: 1203 4246 5223 1203
Size: 3043 977 1327
Fragments arranged by size:
3043 1327 977
AgeI A'CCGG_T
Cuts at: 4178 4178
Size: 5347
AhdI GACnn_n'nnGTC
Cuts at: 4076 4076
Size: 5347
AleI CACnn'nnGTG
Cuts at: 1166 2285 1166
Size: 1119 4228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5347
AlwNI CAG_nnn'CTG
Cuts at: 2034 2186 2634 3090 4662 2034
Size: 152 448 456 1572 2719
Fragments arranged by size:
2719 1572 456 448 152
ApaLI G'TGCA_C
Cuts at: 3404 4560 3404
Size: 1156 4191
ApoI r'AATT_y
Cuts at: 1493 2656 2882 3034 5143 1493
Size: 1163 226 152 2109 1697
Fragments arranged by size:
2109 1697 1163 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3901 160
Size: 872 2869 1606
Fragments arranged by size:
2869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
AvaI C'yCGr_G
Cuts at: 1870 1943 2354 2672 1870
Size: 73 411 318 4545
Fragments arranged by size:
4545 411 318 73
BamHI G'GATC_C
Cuts at: 2677 3044 2677
Size: 367 4980
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2668 4123 618
Size: 325 217 627 306 575 1455 1842
Fragments arranged by size:
1842 1455 627 575 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2361 2666 1391
Size: 970 305 4072
Fragments arranged by size:
4072 970 305
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4376
BbvCI CC'TCA_GC
Cuts at: 2258 2258
Size: 5347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3538 3572 1903
Size: 34 1601 34 3678
Fragments arranged by size:
3678 1601 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3239 4449 3239
Size: 1210 4137
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3958 136
Size: 107 122 71 631 605 2286 1525
Fragments arranged by size:
2286 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5342 5342
Size: 5347
BlpI GC'TnA_GC
Cuts at: 2764 2764
Size: 5347
Bme1580I G_kGCm'C
Cuts at: 1790 2160 3408 4564 1790
Size: 370 1248 1156 2573
Fragments arranged by size:
2573 1248 1156 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5347
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 4036 442
Size: 416 53 83 399 24 1661 958 1753
Fragments arranged by size:
1753 1661 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4007 1239
Size: 2768 2579
Bpu10I CC'TnA_GC
Cuts at: 2258 2258
Size: 5347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 3472 4337 4635 2345
Size: 1127 865 298 3057
Fragments arranged by size:
3057 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 4010 882
Size: 617 1056 1455 2219
Fragments arranged by size:
2219 1455 1056 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 2380 3138 493
Size: 711 951 225 758 2702
Fragments arranged by size:
2702 951 758 711 225
BsaBI GATnn'nnATC
Cuts at: 5341 5341
Size: 5347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 3154 3536 4171
Size: 53 83 186 936 603 1018 382 635
Cuts at: 4171 275
Size: 1451
Fragments arranged by size:
1451 1018 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3780 4178 4452 4599 5010 3780
Size: 398 274 147 411 4117
Fragments arranged by size:
4117 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5122
Fragments arranged by size:
5122 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5347
BseYI C'CCAG_C
Cuts at: 4550 5046 4550
Size: 496 4851
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4774
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2505 2797 3558 4586 1540
Size: 275 186 504 292 761 1028 2301
Fragments arranged by size:
2301 1028 761 504 292 275 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2512 3408 3493 4564 2361
Size: 151 896 85 1071 3144
Fragments arranged by size:
3144 1071 896 151 85
BsmI GAATG_Cn'
Cuts at: 2858 2951 2858
Size: 93 5254
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2361 2512 2666 3408 3493 4564
Size: 399 370 201 151 154 742 85 1071
Cuts at: 4564 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 370 201 154 151
85
BspEI T'CCGG_A
Cuts at: 5010 5010
Size: 5347
BspHI T'CATG_A
Cuts at: 3236 4966 3236
Size: 1730 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2702 844
Size: 1273 585 3489
Fragments arranged by size:
3489 1273 585
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 1394
Size: 429 1411 3507
Fragments arranged by size:
3507 1411 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3842 4016 65
Size: 1183 2594 174 1396
Fragments arranged by size:
2594 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3991 4178 1700
Size: 110 2181 187 2869
Fragments arranged by size:
2869 2181 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5347
BssSI C'ACGA_G
Cuts at: 3407 4419 3407
Size: 1012 4335
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2212 794
Size: 1418 3929
BstBI TT'CG_AA
Cuts at: 4151 4151
Size: 5347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5347
BstYI r'GATC_y
Cuts at: 1972 2677 3044 3429 3446 4898 4984 4996 5342
Size: 705 367 385 17 1452 86 12 346
Cuts at: 5342 1972
Size: 1977
Fragments arranged by size:
1977 1452 705 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2097 2232 513
Size: 552 1032 135 3628
Fragments arranged by size:
3628 1032 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4608
Fragments arranged by size:
4608 683 56
BtsI GCAGTG_nn'
Cuts at: 2863 3657 3677 2863
Size: 794 20 4533
Fragments arranged by size:
4533 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2149 2184 529
Size: 35 1585 35 3692
Fragments arranged by size:
3692 1585 35 35
DraI TTT'AAA
Cuts at: 2651 2651
Size: 5347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 809
Size: 507 3038 1802
Fragments arranged by size:
3038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2794 3683 5312
Size: 54 1425 50 275 18 964 889 1629
Cuts at: 5312 8
Size: 43
Fragments arranged by size:
1629 1425 964 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2794 1537
Size: 275 982 4090
Fragments arranged by size:
4090 982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3277 1353
Size: 1924 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3934 4308 4454 387
Size: 1064 138 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2359
Size: 5347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3410 4007 4794 5062 1239
Size: 393 1778 597 787 268 1524
Fragments arranged by size:
1778 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2350 2400 2350
Size: 50 5297
EcoRI G'AATT_C
Cuts at: 2656 3034 2656
Size: 378 4969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 5347
FspI TGC'GCA
Cuts at: 3853 3853
Size: 5347
HaeII r_GCGC'y
Cuts at: 1678 4494 1678
Size: 2816 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3988 4020 4062 4094 1373
Size: 32 135 32 2416 32 42 32 2626
Fragments arranged by size:
2626 2416 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2937 669
Size: 988 281 753 246 3079
Fragments arranged by size:
3079 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5347
HpaI GTT'AAC
Cuts at: 2937 2937
Size: 5347
KpnI G_GTAC'C
Cuts at: 2672 2672
Size: 5347
MfeI C'AATT_G
Cuts at: 2946 2946
Size: 5347
MluI A'CGCG_T
Cuts at: 5223 5223
Size: 5347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4461 4645 1305
Size: 3156 184 2007
Fragments arranged by size:
3156 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3525
Fragments arranged by size:
3525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3305 3664 3823 518
Size: 648 1119 1020 359 159 2042
Fragments arranged by size:
2042 1119 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2234 2257 2631 2763 3440 4588 4833
Size: 428 200 23 374 132 677 1148 245
Cuts at: 4833 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 428 374 245 200 132
23
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4795
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5347
NotI GC'GGCC_GC
Cuts at: 2794 2794
Size: 5347
NspI r_CATG'y
Cuts at: 1132 1229 2374 2705 4250 1132
Size: 97 1145 331 1545 2229
Fragments arranged by size:
2229 1545 1145 331 97
PasI CC'CwG_GG
Cuts at: 1348 2144 1348
Size: 796 4551
PciI A'CATG_T
Cuts at: 4246 4246
Size: 5347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5347
PfoI T'CCnGG_A
Cuts at: 2337 2337
Size: 5347
PmeI GTTT'AAAC
Cuts at: 2651 2651
Size: 5347
PmlI CAC'GTG
Cuts at: 2380 2380
Size: 5347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 4929 4961 3416
Size: 32 1481 32 3802
Fragments arranged by size:
3802 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2350 2400 2350
Size: 50 5297
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5347
PsiI TTA'TAA
Cuts at: 2917 2917
Size: 5347
PstI C_TGCA'G
Cuts at: 830 2045 2699 830
Size: 1215 654 3478
Fragments arranged by size:
3478 1215 654
PvuI CG_AT'CG
Cuts at: 2001 2505 2001
Size: 504 4843
PvuII CAG'CTG
Cuts at: 2034 2257 2631 2034
Size: 223 374 4750
Fragments arranged by size:
4750 374 223
SacI G_AGCT'C
Cuts at: 2361 2361
Size: 5347
SacII CC_GC'GG
Cuts at: 2235 2235
Size: 5347
SalI G'TCGA_C
Cuts at: 1936 2689 1936
Size: 753 4594
SanDI GG'GwC_CC
Cuts at: 2350 2400 2350
Size: 50 5297
SbfI CC_TGCA'GG
Cuts at: 2699 2699
Size: 5347
ScaI AGT'ACT
Cuts at: 3595 5307 3595
Size: 1712 3635
SfcI C'TryA_G
Cuts at: 826 1045 2041 2695 3830 4511 4702 826
Size: 219 996 654 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 681 654 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5347
SmaI CCC'GGG
Cuts at: 1872 2674 1872
Size: 802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 3451 4352 4614 820
Size: 197 926 417 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 3138 493
Size: 2645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5347
SphI G_CATG'C
Cuts at: 1132 2374 2705 1132
Size: 1242 331 3774
Fragments arranged by size:
3774 1242 331
SspI AAT'ATT
Cuts at: 5 52 3271 5
Size: 47 3219 2081
Fragments arranged by size:
3219 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5347
StyI C'CwwG_G
Cuts at: 513 1065 2433 2786 513
Size: 552 1368 353 3074
Fragments arranged by size:
3074 1368 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 3391 3544 3729 4174 325
Size: 1255 1794 17 153 185 445 1498
Fragments arranged by size:
1794 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3593 5305 96
Size: 274 80 33 51 1443 1616 1712 138
Fragments arranged by size:
1712 1616 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5307 5339 5307
Size: 32 5315
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5347
XbaI T'CTAG_A
Cuts at: 2683 2683
Size: 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5347
XmaI C'CCGG_G
Cuts at: 1870 2672 1870
Size: 802 4545
XmnI GAAnn'nnTTC
Cuts at: 3476 3476
Size: 5347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3155 276
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI XcmI