Restriction map for
pFN21AB3993
(Circular) (Six-base) MAPSORT of: pFN21AB3993.seq Check: 1872 from: 1 to: 5959
pFN21AB3993 5959 bp
With 182 enzymes: SgfI *
November 16, 2017 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2109 3120 2109
Size: 1011 4948
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2419 4022 5406 5674 1632
Size: 787 1603 1384 268 1917
Fragments arranged by size:
1917 1603 1384 787 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2320 2836 2857 3187 3702 5274 2320
Size: 516 21 330 515 1572 3005
Fragments arranged by size:
3005 1572 516 515 330 21
ApaI G_GGCC'C
Cuts at: 2024 2032 2052 2126 2024
Size: 8 20 74 5857
Fragments arranged by size:
5857 74 20 8
ApaLI G'TGCA_C
Cuts at: 3155 4016 5172 3155
Size: 861 1156 3942
Fragments arranged by size:
3942 1156 861
ApoI r'AATT_y
Cuts at: 1493 2039 2926 3268 3494 3646 5755 1493
Size: 546 887 342 226 152 2109 1697
Fragments arranged by size:
2109 1697 887 546 342 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2159 3168 3284 1870
Size: 73 82 134 1009 116 4545
Fragments arranged by size:
4545 1009 134 116 82 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2533 2566 2533
Size: 33 5926
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2485 2803 3280 4735
Size: 325 217 627 270 428 318 477 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 627 477 428 325 318 270
217
BanII G_rGCy'C
Cuts at: 1391 2024 2032 2052 2126 2183 2541 3278 1391
Size: 633 8 20 74 57 358 737 4072
Fragments arranged by size:
4072 737 633 358 74 57 20 8
BbeI G_GCGC'C
Cuts at: 2061 2061
Size: 5959
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2601 4570 136
Size: 107 122 71 631 605 929 1969 1525
Fragments arranged by size:
1969 1525 929 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2032 2052 2126 2808 3159 4020 5176
Size: 234 8 20 74 682 351 861 1156
Cuts at: 5176 1790
Size: 2573
Fragments arranged by size:
2573 1156 861 682 351 234 74 20
8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 2824 3690
Size: 416 53 83 399 24 1048 359 866
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 1048 958 866 416 399 359 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 4619 1239
Size: 947 2433 2579
Fragments arranged by size:
2579 2433 947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3002 4084 4949 5247 3002
Size: 1082 865 298 3714
Fragments arranged by size:
3714 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2978 3057 4622 882
Size: 617 1479 79 1565 2219
Fragments arranged by size:
2219 1565 1479 617 79
BsaAI yAC'GTr
Cuts at: 493 1204 2704 3750 493
Size: 711 1500 1046 2702
Fragments arranged by size:
2702 1500 1046 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 3766 4148 4783
Size: 53 83 186 936 525 1708 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1708 1451 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 1178
Size: 30 165 30 756 30 4948
Fragments arranged by size:
4948 756 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 3122 1186
Size: 1555 381 4023
Fragments arranged by size:
4023 1555 381
BseYI C'CCAG_C
Cuts at: 2137 2347 2604 2975 5162 5658 2137
Size: 210 257 371 2187 496 2438
Fragments arranged by size:
2438 2187 496 371 257 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 4297
Fragments arranged by size:
4297 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3159 4020 4105 5176 2541
Size: 618 861 85 1071 3324
Fragments arranged by size:
3324 1071 861 618 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2032 2052 2126 2183 2541 2808
Size: 399 234 8 20 74 57 358 267
Cuts at: 2808 3159 3278 4020 4105 5176 1391
Size: 351 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 351 267 234
119 85 74 57 20 8
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2452 3120 3314 844
Size: 1265 343 668 194 3489
Fragments arranged by size:
3489 1265 668 343 194
BsrBI CCG'CTC
Cuts at: 1394 1823 3178 3846 1394
Size: 429 1355 668 3507
Fragments arranged by size:
3507 1355 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2242 3222 4603 4790 1700
Size: 110 432 980 1381 187 2869
Fragments arranged by size:
2869 1381 980 432 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2129 2246 1925
Size: 204 117 5638
Fragments arranged by size:
5638 204 117
BssSI C'ACGA_G
Cuts at: 2723 4019 5031 2723
Size: 1296 1012 3651
Fragments arranged by size:
3651 1296 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3187 794
Size: 2393 3566
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 2677
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954
Size: 1317 367 385 17 1452 86 12 346
Cuts at: 5954 1972
Size: 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2183 2745 2868 2917 513
Size: 552 939 57 122 562 123 49 3555
Fragments arranged by size:
3555 939 562 552 123 122 57 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3188 3475 4269 4289 3188
Size: 287 794 20 4858
Fragments arranged by size:
4858 794 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2667 3406
Size: 54 1425 50 275 18 426 411 739
Cuts at: 3406 4295 5924 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 739 426 411 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3022 3032 4546 4920 5066 387
Size: 1064 138 1433 10 1514 374 146 1280
Fragments arranged by size:
1514 1433 1280 1064 374 146 138 10
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2419 4022 4619 5406 5674 1239
Size: 393 554 233 1603 597 787 268 1524
Fragments arranged by size:
1603 1524 787 597 554 393 268 233
EcoO109I rG'GnC_Cy
Cuts at: 2020 2021 2029 2048 2049 2123 2317 2608 2020
Size: 1 8 19 1 74 194 291 5371
Fragments arranged by size:
5371 291 194 74 19 8 1 1
EcoRI G'AATT_C
Cuts at: 2039 2926 3268 3646 2039
Size: 887 342 378 4352
Fragments arranged by size:
4352 887 378 342
EcoRV GAT'ATC
Cuts at: 2752 2752
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2061 2192 2231 2381 5106 1678
Size: 383 131 39 150 2725 2531
Fragments arranged by size:
2725 2531 383 150 131 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 2580 2612 3054
Size: 32 135 32 951 32 25 32 442
Cuts at: 3054 3086 4600 4632 4674 4706 1373
Size: 32 1514 32 42 32 2626
Fragments arranged by size:
2626 1514 951 442 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2057 2057
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 10
Size: 54 1425 343 837 3300
Fragments arranged by size:
3300 1425 837 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3917 4276 4435 518
Size: 648 1578 1173 359 159 2042
Fragments arranged by size:
2042 1578 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2063 2360 3375 4052 5200 5445 1606
Size: 429 28 297 1015 677 1148 245 2120
Fragments arranged by size:
2120 1148 1015 677 429 297 245 28
NaeI GCC'GGC
Cuts at: 2244 3224 2244
Size: 980 4979
NarI GG'CG_CC
Cuts at: 2058 2058
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 2004 2745 2868 513
Size: 552 939 741 123 3604
Fragments arranged by size:
3604 939 741 552 123
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NgoMIV G'CCGG_C
Cuts at: 2242 3222 2242
Size: 980 4979
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2053 2857 1348
Size: 705 804 4450
Fragments arranged by size:
4450 804 705
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 4747
PfoI T'CCnGG_A
Cuts at: 2382 2382
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 2283 1613
Size: 670 5289
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2020 2028 2048 2122 2020
Size: 8 20 74 5857
Fragments arranged by size:
5857 74 20 8
PstI C_TGCA'G
Cuts at: 830 2223 2826 3192 3311 830
Size: 1393 603 366 119 3478
Fragments arranged by size:
3478 1393 603 366 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 5959
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 2223 2826 3311 2223
Size: 603 485 4871
Fragments arranged by size:
4871 603 485
ScaI AGT'ACT
Cuts at: 3218 4207 5919 3218
Size: 989 1712 3258
Fragments arranged by size:
3258 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 2219 2815 2822 2994 3188 3307 4442
Size: 219 1174 596 7 172 194 119 1135
Cuts at: 4442 5123 5314 826
Size: 681 191 1471
Fragments arranged by size:
1471 1174 1135 681 596 219 194 191
172 119 7
SfoI GGC'GCC
Cuts at: 2059 2059
Size: 5959
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2027 2161 3170 3286 1872
Size: 155 134 1009 116 4545
Fragments arranged by size:
4545 1009 155 134 116
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 4063 4964 5226 820
Size: 197 926 1038 1082 901 262 1553
Fragments arranged by size:
1553 1082 1038 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SrfI GCCC'GGGC
Cuts at: 2161 2161
Size: 5959
SspI AAT'ATT
Cuts at: 5 52 2573 3883 5
Size: 47 2521 1310 2081
Fragments arranged by size:
2521 2081 1310 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2745 2868 3398 513
Size: 552 939 396 345 123 530 3074
Fragments arranged by size:
3074 939 552 530 396 345 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 2791 3015 3986 4003 4156 4341
Size: 1255 1116 95 224 971 17 153 185
Cuts at: 4341 4786 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1116 971 445 224 185 153
95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3216 4205 5917
Size: 274 80 33 51 1443 1239 989 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1443 1239 989 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 5919 5951 2159
Size: 32 3728 32 2167
Fragments arranged by size:
3728 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2025 2159 3168 3284 1870
Size: 155 134 1009 116 4545
Fragments arranged by size:
4545 1009 155 134 116
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SwaI XcmI