Restriction map for
pFN21AB3985
(Circular) (Six-base) MAPSORT of: pFN21AB3985.seq Check: 811 from: 1 to: 5713
pFN21AB3985 5713 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2559 3056 1937
Size: 622 497 4594
Fragments arranged by size:
4594 622 497
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2784 3776 5160 5428 1632
Size: 989 163 992 1384 268 1917
Fragments arranged by size:
1917 1384 992 989 268 163
AfeI AGC'GCT
Cuts at: 2046 2046
Size: 5713
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 4612 5589 1203
Size: 3409 977 1327
Fragments arranged by size:
3409 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2549 1184
Size: 1365 4348
AlwNI CAG_nnn'CTG
Cuts at: 2289 2590 3456 5028 2289
Size: 301 866 1572 2974
Fragments arranged by size:
2974 1572 866 301
ApaI G_GGCC'C
Cuts at: 2522 2522
Size: 5713
ApaLI G'TGCA_C
Cuts at: 2059 3770 4926 2059
Size: 1711 1156 2846
Fragments arranged by size:
2846 1711 1156
ApoI r'AATT_y
Cuts at: 1493 3022 3248 3400 5509 1493
Size: 1529 226 152 2109 1697
Fragments arranged by size:
2109 1697 1529 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 2207 3038 1870
Size: 73 264 831 4545
Fragments arranged by size:
4545 831 264 73
BamHI G'GATC_C
Cuts at: 2457 2785 3043 3410 2457
Size: 328 258 367 4760
Fragments arranged by size:
4760 367 328 258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 2764 3034 4489 618
Size: 325 217 627 711 266 270 1455 1842
Fragments arranged by size:
1842 1455 711 627 325 270 266 217
BanII G_rGCy'C
Cuts at: 1391 2522 2649 2736 3032 1391
Size: 1131 127 87 296 4072
Fragments arranged by size:
4072 1131 296 127 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 4626
Fragments arranged by size:
4626 971 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3904 3938 1903
Size: 34 1967 34 3678
Fragments arranged by size:
3678 1967 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2234 3605 4815 2234
Size: 1371 1210 3132
Fragments arranged by size:
3132 1371 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 4324 136
Size: 107 122 71 631 605 422 2230 1525
Fragments arranged by size:
2230 1525 631 605 422 122 107 71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 2696 3130 2696
Size: 434 5279
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2296 2501 2522 2769 2929 3774 4930
Size: 273 233 205 21 247 160 845 1156
Cuts at: 4930 1790
Size: 2573
Fragments arranged by size:
2573 1156 845 273 247 233 205 160
21
BmgBI CAC'GTC
Cuts at: 1318 2407 1318
Size: 1089 4624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2932 3444 4402
Size: 416 53 83 399 24 1515 512 958
Cuts at: 4402 442
Size: 1753
Fragments arranged by size:
1753 1515 958 512 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2750 4373 1239
Size: 1511 1623 2579
Fragments arranged by size:
2579 1623 1511
Bpu10I CC'TnA_GC
Cuts at: 2308 2579 2308
Size: 271 5442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3838 4703 5001 3838
Size: 865 298 4550
Fragments arranged by size:
4550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 493
Size: 711 2300 2702
Fragments arranged by size:
2702 2300 711
BsaBI GATnn'nnATC
Cuts at: 2790 5707 2790
Size: 2917 2796
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 2379 3520 3902
Size: 53 83 186 936 620 226 1141 382
Cuts at: 3902 4537 275
Size: 635 1451
Fragments arranged by size:
1451 1141 936 635 620 382 226 186
83 53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5488
Fragments arranged by size:
5488 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5713
BseYI C'CCAG_C
Cuts at: 2196 2522 4916 5412 2196
Size: 326 2394 496 2497
Fragments arranged by size:
2497 2394 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 1163
Size: 573 570 4570
Fragments arranged by size:
4570 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3924 4952 1540
Size: 275 186 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2063 2649 2736 3774 3859 4930 2063
Size: 586 87 1038 85 1071 2846
Fragments arranged by size:
2846 1071 1038 586 87 85
BsmI GAATG_Cn'
Cuts at: 2440 3224 3317 2440
Size: 784 93 4836
Fragments arranged by size:
4836 784 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2296 2501 2522 2649 2736 2769
Size: 399 273 233 205 21 127 87 33
Cuts at: 2769 2929 3032 3774 3859 4930 1391
Size: 160 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 273 233 205 160
127 103 87 85 33 21
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 2610 3602 5332 2610
Size: 992 1730 2991
Fragments arranged by size:
2991 1730 992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2577 3068 844
Size: 1643 90 491 3489
Fragments arranged by size:
3489 1643 491 90
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 3600 1394
Size: 429 284 1493 3507
Fragments arranged by size:
3507 1493 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 4208 4382 65
Size: 1183 2960 174 1396
Fragments arranged by size:
2960 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 2176 4357 4544 1700
Size: 110 352 14 2181 187 2869
Fragments arranged by size:
2869 2181 352 187 110 14
BsrGI T'GTAC_A
Cuts at: 96 2079 2843 96
Size: 1983 764 2966
Fragments arranged by size:
2966 1983 764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 3773 4785 3773
Size: 1012 4701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 4187
BstBI TT'CG_AA
Cuts at: 4517 4517
Size: 5713
BstEII G'GTnAC_C
Cuts at: 1184 2299 1184
Size: 1115 4598
BstYI r'GATC_y
Cuts at: 1972 2457 2785 3043 3410 3795 3812 5264 5350
Size: 485 328 258 367 385 17 1452 86
Cuts at: 5350 5362 5708 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 328 258
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2426 2426
Size: 5713
BtgI C'CryG_G
Cuts at: 513 1065 2023 2217 2225 513
Size: 552 958 194 8 4001
Fragments arranged by size:
4001 958 552 194 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2419 487
Size: 683 56 1193 3781
Fragments arranged by size:
3781 1193 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 2278 3229 4023 4043 2040
Size: 238 951 794 20 3710
Fragments arranged by size:
3710 951 794 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5678
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DraIII CAC_nnn'GTG
Cuts at: 2058 2058
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2157 2228
Size: 54 1425 50 275 18 265 62 71
Cuts at: 2228 3160 4049 5678 8
Size: 932 889 1629 43
Fragments arranged by size:
1629 1425 932 889 275 265 71 62
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3160 1537
Size: 275 1348 4090
Fragments arranged by size:
4090 1348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2654 3643 1353
Size: 1301 989 3423
Fragments arranged by size:
3423 1301 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2198 2270 4300 4674 4820 387
Size: 1064 138 609 72 2030 374 146 1280
Fragments arranged by size:
2030 1280 1064 609 374 146 138 72
EcoICRI GAG'CTC
Cuts at: 2647 2734 2647
Size: 87 5626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 2750 2784 3776 4373 5160 5428
Size: 393 989 129 34 992 597 787 268
Cuts at: 5428 1239
Size: 1524
Fragments arranged by size:
1524 992 989 787 597 393 268 129
34
EcoNI CCTnn'n_nnAGG
Cuts at: 2582 2582
Size: 5713
EcoO109I rG'GnC_Cy
Cuts at: 2212 2518 2519 2212
Size: 306 1 5406
Fragments arranged by size:
5406 306 1
EcoRI G'AATT_C
Cuts at: 3022 3400 3022
Size: 378 5335
EcoRV GAT'ATC
Cuts at: 2682 2682
Size: 5713
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 2048 2206 4860 1678
Size: 370 158 2654 2531
Fragments arranged by size:
2654 2531 370 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4354 4386 4428 4460 1373
Size: 32 135 32 2782 32 42 32 2626
Fragments arranged by size:
2782 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3303 669
Size: 988 281 1119 246 3079
Fragments arranged by size:
3079 1119 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 2721 3312 2721
Size: 591 5122
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2709 4827 5011 1305
Size: 1404 2118 184 2007
Fragments arranged by size:
2118 2007 1404 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3891
Fragments arranged by size:
3891 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2369 2817 3671 4030 4189 518
Size: 648 888 315 448 854 359 159 2042
Fragments arranged by size:
2042 888 854 648 448 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 2219 2227 3129 3806 4954 5199 1606
Size: 613 8 902 677 1148 245 2120
Fragments arranged by size:
2120 1148 902 677 613 245 8
NaeI GCC'GGC
Cuts at: 2178 2178
Size: 5713
NcoI C'CATG_G
Cuts at: 513 1065 2023 513
Size: 552 958 4203
Fragments arranged by size:
4203 958 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NgoMIV G'CCGG_C
Cuts at: 2176 2176
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 2536 3071 4616 1132
Size: 97 1307 535 1545 2229
Fragments arranged by size:
2229 1545 1307 535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5713
PciI A'CATG_T
Cuts at: 4612 4612
Size: 5713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 2691 1108
Size: 921 662 4130
Fragments arranged by size:
4130 921 662
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2212
Size: 5713
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PspOMI G'GGCC_C
Cuts at: 2518 2518
Size: 5713
PstI C_TGCA'G
Cuts at: 830 2285 2637 2783 3065 830
Size: 1455 352 146 282 3478
Fragments arranged by size:
3478 1455 352 282 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
SacI G_AGCT'C
Cuts at: 2649 2736 2649
Size: 87 5626
SacII CC_GC'GG
Cuts at: 2220 2228 2220
Size: 8 5705
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SanDI GG'GwC_CC
Cuts at: 2212 2212
Size: 5713
SapI GCTCTTCn'nnn_
Cuts at: 2654 2654
Size: 5713
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2281 2633 2779 3061 4196 4877 5068
Size: 219 1236 352 146 282 1135 681 191
Cuts at: 5068 826
Size: 1471
Fragments arranged by size:
1471 1236 1135 681 352 282 219 191
146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 2209 3040 1872
Size: 337 831 4545
Fragments arranged by size:
4545 831 337
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4718 4980 820
Size: 197 926 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 2941 3637 5
Size: 47 2889 696 2081
Fragments arranged by size:
2889 2081 696 47
StuI AGG'CCT
Cuts at: 1517 2606 1517
Size: 1089 4624
StyI C'CwwG_G
Cuts at: 513 1065 2023 3152 513
Size: 552 958 1129 3074
Fragments arranged by size:
3074 1129 958 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2210 3740 3757 3910 4095 4540 325
Size: 1255 630 1530 17 153 185 445 1498
Fragments arranged by size:
1530 1498 1255 630 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2843 3959
Size: 274 80 33 51 1443 102 764 1116
Cuts at: 3959 5671 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1116 764 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 2207 3038 1870
Size: 337 831 4545
Fragments arranged by size:
4545 831 337
XmnI GAAnn'nnTTC
Cuts at: 2606 3842 2606
Size: 1236 4477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SexAI SfiI SfoI SrfI SwaI
XcmI